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http://dx.doi.org/10.5352/JLS.2012.22.8.1034

Quantitative Analyses of Cells using Photoshop after the H&E Staining of the Synovia of Osteoarthritis and Rheumatoid Arthritis Patients  

Park, Jin-Ah (Department of Biological Sciences, College of Natural Sciences, Kangwon National University)
Kim, Keun-Cheol (Department of Biological Sciences, College of Natural Sciences, Kangwon National University)
Publication Information
Journal of Life Science / v.22, no.8, 2012 , pp. 1034-1040 More about this Journal
Abstract
Synovium is the soft tissue that lines the non-cartilaginous surfaces within joints. It has been reported that synovial cells are activated during the pathogenesis of rheumatoid arthritis. In this study, we quantitate and compare the cellular composition of synovia derived from individuals with non-inflammatory osteoarthritis (OA) and those with inflammatory rheumatoid arthritis (RA). Synovia from OA (n=8) and RA (n=5) patients were used for hematoxylin and eosin (H&E) staining. A light microscopic examination has shown that RA synovia were morphologically thickened and hypertrophied as compared to OA synovia. We also performed an immunohistochemistry (IHC) analysis to classify cell types in the synovia using CD68, CD90, or PGP9.5 markers. As a result, we obtained quantitative data regarding the cell populations, which are macrophages in the lining layer and FLSs in the subintimal layer of the synovium. Further Photoshop analyses of the H&E images could allow the counting of the number and layer of the cells in the synovium. The number and layers of the macrophage cells were increased in the lining layer of the RA synovia as compared to the OA synovia. FLS cells also were increased in the subintimal layer of RA synovia. Therefore, quantification of the H&E stained images via Photoshop is a possible analysis protocol for synovium study. This quantitation also supports the idea that the increases in cell number and cell activation are important processes for RA pathogenesis.
Keywords
Synovium; arthritis; hematoxylin and eosin (H&E) staining; immunohistochemistry(IHC); photoshop;
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1 Abramson, S. B. and Attur, M. 2009. Developments in the scientific understanding of osteoarthritis. Arthritis Res. Ther. 11, 227.   DOI
2 D'Arcy, P., Ryan, B. A. and Brodin, B. 2009. Reactivation of p53 function in synovial sarcoma cells by inhibition of p53-HDM2 interaction. Cancer Lett. 275, 285-292.   DOI
3 Farahat, M. N., Yanni, G., Poston, R. and Panayi, G. S. 1993. Cytokine expression in synovial membranes of patients with rheumatoid arthritis and osteoarthritis. Ann. Rheum. Dis. 52, 870-875.   DOI
4 Fearon, U., Griosios, K., Fraser, A., Reece, R., Emery, P., Jones, P. F. and Veale, D. J. 2003. Angiopoietins, growth factors, and vascular morphology in early arthritis. J. Rheumatol. 30, 260-268.
5 Fox, D. A., Gizinski, A., Morgan, R. and Lundy, S. K. 2010. Cell-cell interactions in rheumatoid arthritis synovium. Rheum. Dis. Clin. North. Am. 36, 311-323.   DOI
6 Gruber, H. E., Ingram, J. A., Hoelscher, G. L., Zinchenko, N., Norton, H. J. and Hanley, E. N. Jr. 2009. Matrix metalloproteinase 28, a novel matrix metalloproteinase, is constitutively expressed in human intervertebral disc tissue and is present in matrix of more degenerated discs. Arthritis Res. Ther. 11, R184.   DOI
7 Kim, W. U., Kang, S. S., Yoo, S. A., Hong, K. H., Bae, D. G., Lee, M. S., Hong, S. W., Chae, C. B. and Cho, C. S. 2006. Interaction of Vascular Endothelial Growth Factor 165 with Neuropilin-1 Protects Rheumatoid Synoviocytes from Apoptotic Death by Regulating Bcl-2 Expression and Bax Translocation. J. Immunol. 177, 5727-5735.   DOI
8 Kunisch, E., Fuhrmann, R., Roth, A., Winter, R., Lungershausen, W. and Kinne, R. W. 2004. Macrophage specificity of three anti-CD68 monoclonal antibodies (KP1, EBM11, and PGM1) widely used for immunohistochemistry and flow cytometry. Ann. Rheum. Dis. 63, 774-784.   DOI
9 Lee, S. H. 2005. Role of synovial fibroblasts in rheumatoid arthritis. Hanyang Medical Reviews 25, 36-42.
10 Liu, H., Perlman, H., Hoffmann, A., Thimmapaya, B. and Richard, M. P. 2000. Regulation of IL-6 and IL-8 expression in rheumatoid arthritis synovial fibroblasts: the dominant role for NF-kB but not C/EBPb or c-Jun. J. Immunol. 165, 7199-7206.   DOI
11 Lundy, S. K., Sarkar, S., Tesmer, L. A. and Fox, D. A. 2007. Cells of the synovium in rheumatoid arthritis. T slynphocytes. Arthritis Res. Ther. 9, 202.   DOI
12 Singh, J., Arayssi, T., Duray, P. and Schumacher, H. 2004. Immunohistochemistry of normal human knee synovium: a quantitative study. Ann. Rheum. Dis. 63, 785-790.   DOI   ScienceOn
13 Myers, S. L., Flusser, D., Brandt, K. D. and Heck, D. A. 1992. Prevalence of cartilage shards in synovium and their association with synovitis in patients with early and endstage osteoarthritis. J. Rheumatol. 19, 1247-1251.
14 Nakano, K., Yamaoka, K., Hanami, K., Saito, K., Sasaguri, Y., Yanagihara, N., Tanaka, S., Katsuki, I., Matsushita, S. and Tanaka, Y. 2011. Dopamine induces IL-6-dependent IL-17 production via D1-like receptor on CD4 naive T cells and D1-like receptor antagonist SCH-23390 inhibits cartilage destruction in a human rheumatoid arthritis/SCID mouse chimera model. J. Immunol. 186, 3745-3752.   DOI
15 Pessler, F., Dai, L., Diaz-Torne, C., Gomez-Vaquero, C., Paessler, M. E., Zheng, D. H., Einhorn, E., Range, U., Scanzello, C. and Schumacher, H. R. 2008. The synovitis of ''on-inflammatory''orthopaedic arthropathies: a quantitative histological and immunohistochemical analysis. Ann. Rheum. Dis. 67, 1184-1187.   DOI
16 Smith, J. B. and Haynes, M. K. 2002. Rheumatoid arthritismolecular understanding. Ann. Intern. Med. 136, 908-912.   DOI   ScienceOn