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http://dx.doi.org/10.5762/KAIS.2011.12.7.3310

The Mechanism of Hematoxylin on Glucose Metabolism Improvement in Sciatic Nerves from Streptozotocin-induced Diabetic Rats  

Chung, Myung-Kiu (Department of Environmental Engineering, Sunmoon University)
Kang, Soon-Kook (Department of Environmental Engineering, Sunmoon University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.12, no.7, 2011 , pp. 3310-3316 More about this Journal
Abstract
Hematoxylin is the main component of Hematoxylon campechianum which has been utilized in the southern provinces of Korea as a folk remedy for diabetic complications. In the present study, to investigate the hypoglycemic mechanism of hematoxylin, the 2-deoxyglucose uptake and phospholipid metabolism were examined in sciatic nerves from three groups of rats : normal control, diabetic control, diabetic hematoxylin-treated group. Hematoxylin significantly reduced blood glucose levels in diabetic control rats. On a wet weight basis, the nerves from diabetic rats showed a 20% decrease in total phospholipid from that of controls and a relative decrease in phosphatidylinositide. Hematoxylin treatment increased the incorporation rate of 2-[3H] myo-inositol into total phosphoinositids in diabetic rat. The effectiveness were more potent in higher dose hematoxylin-treated rats than lower dose hematoxylin-treated rats. These results suggest that hematoxylin increases glucose transport and lipid metabolism by partially normalizing concerned with myo-inositol metabolism in diabetic rat. Therefore we propose that hematoxylin can be a promising candidate for diabetes medication.
Keywords
Hematoxylin; Diabetes Mellitus; 2-Deoxyglucose Uptake; Sciatic Nerve; Phosphoinositide; Phospholipid Metabolism;
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  • Reference
1 Girolamo M, Howe M, Metabolic pattern and insulin responsiveness of enlarging fat cells, J Lip Res, pp. 332-338, 15, 1974.
2 Kobayashi M, Olefsky JM, Effects of streptozotocininduced diabetes on insulin binding, glucose transport and intracellular glucose metabolism in isolated rat sciatic nerves, Diabetes, pp. 87-95, 28. 1979.   DOI
3 Bell M.E., Metabolism of phospholipids in peripheral nerves from Rats with Chronic Streptozotocin-Induced Diabetes: Increased Turnover,J. Neurochem. pp. 192-199, 39, 1982.   DOI
4 Olefsky JM, Effects of fasting on insulin binding, glucose transport, and glucose oxidation in isolated rat sciatic nerves, J Clin Invest, pp. 1450-1460, 78(7), 1976.
5 Garbus J.,Cryer PE: The metabolic impact of autonomic neuropathy in insulin -dependent diabetes mellitus. J. biol. Chem. pp. 59-65, 238, 1973.
6 Green D. A. :Metabolic abnormalities in diabetics peripheral nerves. Metabolism. pp. 118-196, 32 1983.
7 Rundulls R.W. : Diabetic neuropathy : General review with report oh 125 cases. Medicine, pp. 111-117, 24, 1998.
8 Henry RR, Effects of troglitazone on insulin sensitivity, Diabetic Med, pp. 148-150, 13, 1996.
9 Bakillah A, Grigorova-Borsos, Guillot R, Effect of aldose reductase inhibitor on type IV collagen production by human endothelial cells cultured in high glucose, Diabetologia, pp. 641-648, 39, 1996.   DOI
10 Lea CH, Experiments on the use of antioxidants in edible fats, J Soc Chem Ind, pp. 107-113, 63(1), 1944.
11 Au W, Hsu TC, Studies on the clastogenic effects of biologic stains and dyes, Environ Mutagen, pp. 27-32, 1(1), 1979.   DOI
12 Hirono H, Taguchi T, Henry RR, Effects of troglitazone on insulin sensitivity, Diabetic Med, pp. 148-150, 13, 1996
13 Hidefumi K, Inactivation of viruses by dyes and visible light, Kurume Igkkai Zassi., pp. 1067-1072, 39(8), 1976.
14 Gabor M, Dux E, The effects of hematoxylin and related compounds on capillary resistance, Acta Phisiol Acad Sci Hung, pp. 409-413, 3(3), 1952.
15 Moon CK, Yun YP, Inhibition of lens-aldose reductase activity by hematoxylin and hematoxylin, Planta Med, pp. 66-67, 47(1), 1985.
16 Moon CK, Ha BJ, A study on the antilipidperoxdative effects of hematoxylin and hematoxylin, Kor J Food Hyg, pp.35-42, 2(3), 1987.