• 한국동물학회지
• /
• 제38권3호
• /
• pp.330-339
• /
• 1995

방게의 혈구는 투명혈구, 소과립혈구, 그리고 대과립혈구로 동정되었다. 투명혈구의 모양은 세포질에 비해 큰 핵을 가지며, 세포질에는 난형의 전자밀도가 약한 과립과 소포 및 소포체들이 관찰된다. 소과립혈구는 투명혈구보다 더 작은 핵을 가지며, 세포질에는 잘 발달된 소포체, 골지체, 그리고 작은 원형의 전자밀도가 강한 과립을 갖는다. 대과립혈구는 소과립들이 서로 융합해서 된 전자밀도가 강한 대과립들을 갖는다. 방게의 혈구는 투명혈구로 부터 대과립혈구로 발달한다. 즉 리소솜효소를 갖는 투명혈구의 과립들은 핵막으로 부터 작은 소포들을 형성하며, 이 소포들이 골지체를 통과하면서 전자밀도가 강한 물질로 채워지고, 이 작은 소과립들을 융합하면서 대과립이 된다. 모든 혈구에는 글리코겐 입자들이 산재한다. 대과립혈구들 중 과립의 소멸현상으로 생각되는 이형과립들이 존재한다.

• 대한본초학회지
• /
• 제39권2호
• /
• pp.1-9
• /
• 2024

Objectives : Paeng-hwal is described as an insect herbal medicine used for digestive diseases in the Dong-ui-bo-gam. The origin of this herbal medicine is limited to several small crabs, such as Helice tridens. These crab species cohabitat in the same environment and share similar morphological characteristics, making it very difficult to distinguish and collect the individual species for use in dietary supplements or herbal medicines. This study was conducted to develop a genetic identification tool for discriminating among these closely related small crab species. Methods : CO1 DNA barcode regions of 15 samples from 6 species of small crabs were analyzed to obtain the individual sequences. To identify the correct species, comparative analyses were carried out using the database of the NCBI GenBank and the NIBR. SCAR primers were designed to develop simple and rapid assay methods using inter-species specific sequences. Optimal SCAR assay conditions were established through gradient PCR, and the limit of detection (LOD) was determined. Results : Six species of small crabs (Helicana tridens, Macrophthalmus abbreviatus, Helicana tientsinensis, Helicana wuana, Chiromantes dehaani, and Hemigrapsus penicillatus), which are distributed as Paeng-hwal, were identified through CO1 sequences analysis. We also developed SCAR markers to distinguish between six small crabs at the species level. Furthermore, we established the optimal PCR assay methods and the LOD of each individual species. Conclusions : The rapid and simple SCAR-PCR assay methods were developed to identify the species and control the quality of herbal medicines for Paeng-hwal based on the genetic analyses of CO1 DNA barcodes.

• 수산해양교육연구
• /
• 제18권3호
• /
• pp.209-217
• /
• 2006

갈게(Helice tridens tientsinensis)를 이용하여 만든 게장은 우리의 식생활에 밀접하게 접근되어 있으나 이에 관련된 연구는 미비한 실정이며 지역마다 게장을 만드는데 필요한 장에 여러 가지 양념류가 첨가되어 있어 게장 본래의 화학적 성분 조성을 밝히기 쉽지 않을 것으로 사료된다. 본 연구에서는 갈게장의 식품학적 가치를 밝히고자 갈게 생육질의 화학적 성분 조성과 염지한 갈게장을 $5^{\circ}C$에 저장하여 저장일수(10, 16, 20, 25, 29, 55, 61, 67)에 따른 화학성분의 변화량을 측정한 결과를 요약하면 다음과 같다. 1. 갈게 생육질의 일반성분 함량을 보면 수분(77.12%), 회분(1.96%), 조단백질(18.93%), 조지방(0.26%)이었으나 저장기간 중 수분과 조단백질 함량은 감소하는 경향을 보인 반면 회분과 조지방의 함량은 수분 함량의 감소에 따라 상대적으로 그 함량이 증가하는 경향을 나타내었다. 2. 갈게 생육질의 휘발성 염기질소량은 갈게는 6.56mg/100g로 나타났으며, $5^{\circ}C$에서 저장한 갈게장은 55일 경과 후에도 23.72mg/100g로 신선도를 유지하였으나 저장 61일 후에는 VBN이 31.6mg/100g로 초기부패 상태였다. 따라서 갈게장의 저장은 $5^{\circ}C$에서 55일까지만 가능함을 알 수 있다. 3. 갈게 생육질의 유리아미노산 함량을 보면 arginine(1140.88mg/100g), alanine (311.26mg/100g), proline(214.63mg/100g), serine (113.56mg/100g), taurine(90.80mg/100g)의 순서로 정량되었으며, 갈게장에서는 이들 아미노산의 함량이 저장기간 (10, 16, 20, 25, 29, 55, 61, 67일)중에 대체로 그 함량이 증가하였다. 특히 갈게장을 $5^{\circ}C$에서 55일 저장하였을 때 aspartic acid, glutamic acid, glycine, arginine, threonine, tyrosine, valine, methionine, lysine, isoleucine, leucine 등의 증가율이 높았다. 4. 갈게 생육질의 지방산은 erucic acid (27.37 area%) 함량이 가장 많았으며 그 다음으로 pentadecenoic acid(19.44 area%), oleic acid(17.68 area%), palmitic acid (11.00 area%), stearic acid(6.89 area%), elaidic acid(6.15 area％) 순이었으나 갈게장은 지방산의 종류에 따라 양적변화에 적은 차이는 있었지만 대체로 갈게 생육질 중의 지방산의 함량과 큰 차이는 없었다. 그러나 갈게 생육질보다 palmitoleic acid, heneicosanoic acid 등 불포화지방산은 갈게장이 비교적 많았다.

• Animal Systematics, Evolution and Diversity
• /
• 제23권1호
• /
• pp.69-74
• /
• 2007

First zoea of cyclograpsinine crab Chasmagnathus convexus (De Haan, 1835) belonging to the family Varunidae, is described and illustrated in detail based on laboratory-hatched material from an ovigerous female collected in Seumjingang river mouth, southern Korea. Morphological comparison is made with previous description of C. convexus from Japan, The first zoea of C. convexus can be readily distinguished from those of six species of Cyclograpsus intermedius Ortmann, 1894, Helicana japonica (K. Sakai and Yatsuzuka, 1980), Helicana wuana (Rathbun, 1931), Helice tientsinensis Rathbun, 1931, Helice tridens (De Man, 1835), and Pseudohelice quadrata (Dana, 1851), the other known cyclograpsinine species in Korea by having the lateral carapace spine, a pair of dorsolateral processes on the fourth abdominal somite, the exopod of antenna with three setae, and the exopod of antenna as being 24.7% length to the protopod.

• 한국동물학회지
• /
• 제5권2호
• /
• pp.13-17
• /
• 1962

The author colelcted crabs (Tribe Brachyura ) on the coasts of fourteen western islands of South Korea (Paenyong Do, Taechong Do, Sochong Do, Taeyonpyong Do, Kanghwa Do, Yongjong Do, Chagyak Do, Tokchok To. Moktok To. Pukkyoknyolbi Do. Ong Do, ANmyon Do, Pigum Do, and Taehuksan Do) mainly in summers during a period ranging from 1955 to 1962. Of these Islands, Paeknyong , Taehong, Taeyonpyong, Ong, Anmyon, Pigum and Taehuksan Do are the localities where the crabs were investigated firstly by the present author. The collections made by the author consist of 1779 specimens of crabs involving 9 families. 25 genera, 33 species and 5 subspecies (Table2). 23 species of crabs had been reported hitherto from Tokchok To and 7 from Kanghwa Do. In present paper the author adds 1 species to the fauna of crabs of Tokchok To and 7 to that of Kanghwa Do. In present paper the author adds 1 species to the fauna of crabs of Tokchok To and 7 to that of Kanghwa D. Among 38 species or subspecies of crabs collected, Oregonia gracilis, Cancer amphioetus and Chaesmagnathus convexus are new to the fauna of the west coast of Korea. 57 species of crabs are therefore identified in this area. The author collected Pinnotheres cardii on Taechong Do and Scopimera bitympana on Pigum Do. It became, therefore, clear that these two kinds of crabs occur much wider areas than known hitherto in Korea. The nothern limit of the distribution of Ocypoda stinpsoni and Sesarma haematocheir is Kyungki Bay as known already, that of Helice tridens is ,however, more northern part than Kyungki Bay, since they were collected on Paeknyong Do.

• Animal Systematics, Evolution and Diversity
• /
• 제36권3호
• /
• pp.228-231
• /
• 2020

Pseudohelice subquadrata (Dana, 1851) is endangered due to its restricted habitat; hence, it has been designated as a marine protected species and endangered species by law in Korea. It has been recorded only Jeju-do and Geomun-do, Republic of Korea. The present study, is the first report on a cytochrome c oxidase subunit I DNA barcode for P. subquadrata. The maximum intra-specific genetic distance among all P. subquadrata individuals was found to be 0.5%, whereas inter-genetic distance within the same genus was 17.2-21.5% compared with Helice tientsinensis (Rathbun, 1931), H. tridens (De Haan, 1835), H. epicure (Ng et al., 2018), and Helicana wuana (Rathbun, 1931). Our barcoding data can thus be used as reference for restoration and conservation studies on P. subquadrata, which are designated as marine protected species.

• 한국어병학회지
• /
• 제22권1호
• /
• pp.15-21
• /
• 2009

White spot syndrome virus (WSSV) is a strong causative agent for high mortality in cultured and wild shrimps. From this study, the WSSV prevalence in marine organisms around shrimp farm as well as live feed-mediated transmission of WSSV to farmed shrimps were investigated. Based on nested-PCR method, WSSV was detected in wide array of marine organisms including Perinereis aibuhitensis (81.3% of prevalence rate, 13/16), Enedrias fangi (100%, 16/16), Ruditapes philippinarum (20%, 2/10), crab larvae (100%, 10/10), copepoda (30%, 3/10), Periophthalmus modestus (50%, 5/10), Pachygrapsus crassipes (10%, 1/10), Helice tridens (20%, 2/10) and Neomysis sp. (70%, 7/10). On the other hand, WSSV was not detected in Bullacta exarata, Uca arcuata, and Reishia clavigera. The percent prevalence of WSSV in wild shrimps, Fenneropenaeus chinensis was only 6%, but markedly increased up to 56% after a feeding trial using polychaete worms for one month, indicating that the live feed is one of significant carriers of WSSV to shrimps under practical farming conditions.