Browse > Article
http://dx.doi.org/10.5635/ASED.2020.36.3.036

DNA Barcoding of the Marine Proteced Species Pseudohelice subquadrata (Decapoda, Varunidae, Pseudohelice) from the Korean Waters  

Kim, Ji Min (National Marine Biodiversity Institute of Korea)
Kim, Jong-Gwan (National Marine Biodiversity Institute of Korea)
Kim, So Yeon (National Marine Biodiversity Institute of Korea)
Choi, Woo Yong (National Marine Biodiversity Institute of Korea)
Kim, Hyung Seop (School of Marine Biotechnology, College of Marine Science, Kunsan National University)
Kim, Min-Seop (National Marine Biodiversity Institute of Korea)
Publication Information
Animal Systematics, Evolution and Diversity / v.36, no.3, 2020 , pp. 228-231 More about this Journal
Abstract
Pseudohelice subquadrata (Dana, 1851) is endangered due to its restricted habitat; hence, it has been designated as a marine protected species and endangered species by law in Korea. It has been recorded only Jeju-do and Geomun-do, Republic of Korea. The present study, is the first report on a cytochrome c oxidase subunit I DNA barcode for P. subquadrata. The maximum intra-specific genetic distance among all P. subquadrata individuals was found to be 0.5%, whereas inter-genetic distance within the same genus was 17.2-21.5% compared with Helice tientsinensis (Rathbun, 1931), H. tridens (De Haan, 1835), H. epicure (Ng et al., 2018), and Helicana wuana (Rathbun, 1931). Our barcoding data can thus be used as reference for restoration and conservation studies on P. subquadrata, which are designated as marine protected species.
Keywords
DNA barcode; cytochrome c oxidase subunit I; marine protected species; endangered species;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Bouchard JM, Poupin J, Cleva R, Dumas J, Dinhut V, 2013. Land, mangrove and freshwater decapod crustaceans of mayotte region (Crustacea, Decapoda). Atoll Research Bulletin, 592:1-69. https://doi.org/10.5479/si.00775630.592
2 Elsasser SC, Floyd R, Hebert PDN, Schulte-Hostedde AI, 2009. Species identificaion of North American guinea worms (Nematoda: Dracunculus) with DNA barcoding. Molecular Ecology Resources, 9:707-712. https://doi.org/10.1111/j.1755-0998.2008.02393.x   DOI
3 Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3:294-299.
4 Kim HS, 1973. Illustrated encyclopedia of fauna and flora of Korea. Vol. 14. Anomura: Brachyura. Samwha Publishing Co., Seoul, pp. 1-649.
5 Kim HS, Kim KY, Lee SH, Hong SS, Cho IY, Yi CH, Kim IH, Yoon M, Kim MS, 2018. The complete mitochondrial genome of Pseudohelice subquadrata (Dana, 1851) (Crustacea: Decapoda: Varunidae). Mitochondrial DNA Part B Resources, 4:103-104. https://doi.org/10.1080/23802359.2018.1536491
6 Kimura M, 1980. A simple method of estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16:111-120. https://doi.org/10.1007/BF01731581   DOI
7 Kumar S, Stecher G, Li M, Knyaz C, Tamura K, 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 35:1547-1549. https://doi.org/10.1093/molbev/msy096   DOI
8 Naderloo R, 2017. Family Varunidae H. Milne Edwards, 1853. Atlas of crabs of the Persian Gulf. Springer, Cham, pp. 357-363.
9 National Institute of Biological Resources (NIBR), 2017. Endangered wildlife at a glance. NIBR, Incheon, pp. 326-327.
10 Sakai K, Turkay M, Yang SL, 2006. Revision of the Helice/ Chasmagnathus complex (Crustacea: Decapoda: Brachyura). Abhandlungen der Senckenbergischen Naturforschenden Gesellschaft, 565:1-76.
11 Sun H, Jin Y, Zhang D, Yang S, Li Q, Song D, Zhou K, 2009. Mitochondrial sequence data reveals the phylogeny of the Asian Helice group of crabs (Decapoda: Brachyura: Varunidae). Journal of Zoological Systematics and Evolutionary Research, 47:322-327. https://doi.org/10.1111/j.1439-0469.2008.00509.x   DOI
12 Ng NK, Naruse T, Shin HT. 2018. Helice epicure, a new species of varunid mud crab (Brachyura, Decapoda, Grapsoidea) from the Ryukyus, Japan. Zoological Studies, 57:15. https://doi.org/10.6620/ZS.2018.57-15
13 Ahyoung ST, Lowry JK, Alonso M, Bamber RN, Boxshall GA, Castro P, Gerken S, Karaman GS, Goy JW, Jones DS, Meland K, Rogers DC, Svavarsson J. 2011. Subphylum Crustacea Brunnich, 1772. In: Animal biodiversity: an outline of higher-level classification and survey of taxonomic richness (Ed., Zhang ZQ). Zootaxa, 3148:165-191. https://doi.org/10.11646/zootaxa.3148.1.33   DOI
14 Hajibabaei M, Janzen DH, Burns JM, Hallwachs W, Hebert PDN, 2006. DNA barcodes distinguish species of tropical Lepidoptera. Prceedings of the National Academy of Sciences of the United States of America, 103:968-971. https://doi.org/10.1073/pnas.0510466103
15 Shih HT, Suzuki H, 2008. Taxonomy, phylogeny, and biogeography of the endemic mudflat crab Helice/Chasmagnathus complex (Crustacea: Brachyura: Varunidae) from East Asia. Zoological Studies, 47:114-125.
16 Song JH, Min GS, 2019. First genetic data of Nebalia koreana (Malacostraca, Leptostraca) with DNA barcode divergence among Nebalia species. Animal Systematics, Evolution and Diversity, 1:37-39. https://doi.org/10.5635/ASED.2019.35.1.003
17 Zemlak TS, Ward RD, Connell AD, Holmes BH, Hebert PDN, 2009. DNA barcoding reveals overlooked marine fishes. Molecular Ecology Resources, 9:237-242. https://doi.org/10.1111/j.1755-0998.2009.02649.x   DOI