• The Korean Journal of Zoology
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• v.38 no.3
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• pp.330-339
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• 1995

In Helice tridens tridens, hylaine cells, small granulocytes, and large granulocytes were identified. Features of hyaline cells include a large nucleus in proportion to the cytoiplasm, and weak electron-dense granules of oval shape and vesicles, and endoplasmic reticulum (ER) in the cytoplasm. Small granulocytes have smaller nucleus than that of the hyaline cells, well-developed ER, Golgi complex, and small, round and electron-dense granules in the cytoplasm. Large granulocytes contain large and electron dense granules (ahout 1 $\mu$m) that fused small granules. Hemocytes of Helice tridens tridens differentiated from hyaline cell to large granulocyte granules of hyaline cells have lysosome and make small vesicles from nuclear envelopes. While these vesicles pass through the Golgi complex, they are filled with electron dense matetials, and then fused with the small granules. They eventually matured into large granules. All of hemocytes have the glycogen particles. In the large granulocytes heterogeneouse granules were supposed to occur by disappearance of granules.

• The Korea Journal of Herbology
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• v.39 no.2
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• pp.1-9
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• 2024

Objectives : Paeng-hwal is described as an insect herbal medicine used for digestive diseases in the Dong-ui-bo-gam. The origin of this herbal medicine is limited to several small crabs, such as Helice tridens. These crab species cohabitat in the same environment and share similar morphological characteristics, making it very difficult to distinguish and collect the individual species for use in dietary supplements or herbal medicines. This study was conducted to develop a genetic identification tool for discriminating among these closely related small crab species. Methods : CO1 DNA barcode regions of 15 samples from 6 species of small crabs were analyzed to obtain the individual sequences. To identify the correct species, comparative analyses were carried out using the database of the NCBI GenBank and the NIBR. SCAR primers were designed to develop simple and rapid assay methods using inter-species specific sequences. Optimal SCAR assay conditions were established through gradient PCR, and the limit of detection (LOD) was determined. Results : Six species of small crabs (Helicana tridens, Macrophthalmus abbreviatus, Helicana tientsinensis, Helicana wuana, Chiromantes dehaani, and Hemigrapsus penicillatus), which are distributed as Paeng-hwal, were identified through CO1 sequences analysis. We also developed SCAR markers to distinguish between six small crabs at the species level. Furthermore, we established the optimal PCR assay methods and the LOD of each individual species. Conclusions : The rapid and simple SCAR-PCR assay methods were developed to identify the species and control the quality of herbal medicines for Paeng-hwal based on the genetic analyses of CO1 DNA barcodes.

• Journal of Fisheries and Marine Sciences Education
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• v.18 no.3
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• pp.209-217
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• 2006

In this study, chemical compositions of Helice tridens tientsinensis(Htt, sea crab) and changes of chemical compositions in fermented sea crab tested according to different storage days(10, 16, 20, 25, 29, 55, 61, 67). The average amount of chemical compositions in raw Htt, it contains 77.12% of moisture, 1.96% of ash, 18.93% of crude protein, 0.26% of crude lipid. During storage time the amount of moisture and crude protein decreased, but crude lipid and ash increased. The amount of volatile basic nitrogen in Htt showed 6.56 mg/100g. The fermented sea crab in brine stored at the temperature of $5^{\circ}C$ showed 23.72 mg/100g of freshness even after 55 days. It showed first stage of decomposition(31.69 mg/100g) after 61 days. it is most efficient to store fermented sea crab at a low temperature. The amount of free amino acid composition in fresh Htt, was measured as follow ;arginine(1140.88 mg/100g), alanine(311.26 mg/100g), prorine(214.63 mg/100g), serine(113.56 mg/100g), taurine(90.80 mg/100g). The amount of amino acid in fermented sea crab increased as the storage days increased. Fatty acid contents in fresh Htt showed the largest amount of erucic acid (27.39 area%) and pentadecenoic acid (19.44 area%), oleic acid (17.68 area%), palmitic acid (11.00 area%), stearic acid (6.89 area%), and elaidic acid (6.15 area%) in order. In fermented sea crab, a small change was noticed in quantity, but the obvious increased composions were palmitoleic and heneicosanoic acid etc.

• Animal Systematics, Evolution and Diversity
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• v.23 no.1
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• pp.69-74
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• 2007

First zoea of cyclograpsinine crab Chasmagnathus convexus (De Haan, 1835) belonging to the family Varunidae, is described and illustrated in detail based on laboratory-hatched material from an ovigerous female collected in Seumjingang river mouth, southern Korea. Morphological comparison is made with previous description of C. convexus from Japan, The first zoea of C. convexus can be readily distinguished from those of six species of Cyclograpsus intermedius Ortmann, 1894, Helicana japonica (K. Sakai and Yatsuzuka, 1980), Helicana wuana (Rathbun, 1931), Helice tientsinensis Rathbun, 1931, Helice tridens (De Man, 1835), and Pseudohelice quadrata (Dana, 1851), the other known cyclograpsinine species in Korea by having the lateral carapace spine, a pair of dorsolateral processes on the fourth abdominal somite, the exopod of antenna with three setae, and the exopod of antenna as being 24.7% length to the protopod.

• The Korean Journal of Zoology
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• v.5 no.2
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• pp.13-17
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• 1962

The author colelcted crabs (Tribe Brachyura ) on the coasts of fourteen western islands of South Korea (Paenyong Do, Taechong Do, Sochong Do, Taeyonpyong Do, Kanghwa Do, Yongjong Do, Chagyak Do, Tokchok To. Moktok To. Pukkyoknyolbi Do. Ong Do, ANmyon Do, Pigum Do, and Taehuksan Do) mainly in summers during a period ranging from 1955 to 1962. Of these Islands, Paeknyong , Taehong, Taeyonpyong, Ong, Anmyon, Pigum and Taehuksan Do are the localities where the crabs were investigated firstly by the present author. The collections made by the author consist of 1779 specimens of crabs involving 9 families. 25 genera, 33 species and 5 subspecies (Table2). 23 species of crabs had been reported hitherto from Tokchok To and 7 from Kanghwa Do. In present paper the author adds 1 species to the fauna of crabs of Tokchok To and 7 to that of Kanghwa Do. In present paper the author adds 1 species to the fauna of crabs of Tokchok To and 7 to that of Kanghwa D. Among 38 species or subspecies of crabs collected, Oregonia gracilis, Cancer amphioetus and Chaesmagnathus convexus are new to the fauna of the west coast of Korea. 57 species of crabs are therefore identified in this area. The author collected Pinnotheres cardii on Taechong Do and Scopimera bitympana on Pigum Do. It became, therefore, clear that these two kinds of crabs occur much wider areas than known hitherto in Korea. The nothern limit of the distribution of Ocypoda stinpsoni and Sesarma haematocheir is Kyungki Bay as known already, that of Helice tridens is ,however, more northern part than Kyungki Bay, since they were collected on Paeknyong Do.

• Animal Systematics, Evolution and Diversity
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• v.36 no.3
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• pp.228-231
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• 2020

Pseudohelice subquadrata (Dana, 1851) is endangered due to its restricted habitat; hence, it has been designated as a marine protected species and endangered species by law in Korea. It has been recorded only Jeju-do and Geomun-do, Republic of Korea. The present study, is the first report on a cytochrome c oxidase subunit I DNA barcode for P. subquadrata. The maximum intra-specific genetic distance among all P. subquadrata individuals was found to be 0.5%, whereas inter-genetic distance within the same genus was 17.2-21.5% compared with Helice tientsinensis (Rathbun, 1931), H. tridens (De Haan, 1835), H. epicure (Ng et al., 2018), and Helicana wuana (Rathbun, 1931). Our barcoding data can thus be used as reference for restoration and conservation studies on P. subquadrata, which are designated as marine protected species.

• Journal of fish pathology
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• v.22 no.1
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• pp.15-21
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• 2009

White spot syndrome virus (WSSV) is a strong causative agent for high mortality in cultured and wild shrimps. From this study, the WSSV prevalence in marine organisms around shrimp farm as well as live feed-mediated transmission of WSSV to farmed shrimps were investigated. Based on nested-PCR method, WSSV was detected in wide array of marine organisms including Perinereis aibuhitensis (81.3% of prevalence rate, 13/16), Enedrias fangi (100%, 16/16), Ruditapes philippinarum (20%, 2/10), crab larvae (100%, 10/10), copepoda (30%, 3/10), Periophthalmus modestus (50%, 5/10), Pachygrapsus crassipes (10%, 1/10), Helice tridens (20%, 2/10) and Neomysis sp. (70%, 7/10). On the other hand, WSSV was not detected in Bullacta exarata, Uca arcuata, and Reishia clavigera. The percent prevalence of WSSV in wild shrimps, Fenneropenaeus chinensis was only 6%, but markedly increased up to 56% after a feeding trial using polychaete worms for one month, indicating that the live feed is one of significant carriers of WSSV to shrimps under practical farming conditions.