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Studies on the Naringinase of Mold-[part 1] Screening test of Molds on the Production of Naringinase and some properties of Crude Enzyme of Selected strain- (사상균 Naringin 분해 효소에 관한 연구-[제1보] 우량 균주의 분리 선별과 선별균의 조효소 성질에 관하여-)

  • Ki, Woo-Kyung;Sung, Nack-Kie
    • Applied Biological Chemistry
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    • v.13 no.3
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    • pp.237-242
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    • 1970
  • Fifty strains of mold which isolated from the various sources were screened for the production of Naringinase which hydrolyse naringin, the 7-rhamnoe-glucoside of 4'.5.7. - trihydroxyflavanonin, the main bitter principle of citrus fruits and grape fruits. Of the 4 strains yielded naringinase with significant activity, S-1 strain was selected on the criterion of industrial application, and some properties of crude naringinase of this S-1 was investigated. The results obtained were as follows. 1. Naringenase obtained from S-1 strain has optimum pH range from 3.0 to 5.0 for its activity. 2. Production of naringinase was increased on the addition of naringin to the medium. 3. Hydrolysis of naringin with approporiate concentration of naringinase was carried out linerly up to 80% on the 0.1% substrate solution. 4. The optimum temperature for its activity was $50^{\circ}C$, and this enzyme was inactivated 80% of its total activity at $70^{\circ}C$ for 10 minutes, 40% at $60^{\circ}C$ for 30 minutes. But signifiant decrease of activity were not occurred by heat treatment at $50^{\circ}C$ for 2 hours. 5. Crude enzyme of the naringinase obtained from S-1 strain was competitively inhibited by addition of glucose on the substrate, and inhibitor constant of the glucose on the this enzyme was 1.5 Mol, and inhibition rate were linearly increased according to the increase of sucrose concentration and 56% of its total activity was inhibited at 1 Mol sucrose solution.

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The Effects of Scale Growth Inhibition on Water Pipe using Frequency Driver (Frequency Driver를 이용한 냉온수관의 스케일 방지억제효과)

  • Jang, Mi-Jeong;Sung, Il-Wha
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.4
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    • pp.258-266
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    • 2011
  • Scale induced by hardness materials in water must be controled because of it can be result in remarkable damages of pipeline as well as water quality deterioration. Especially hot water system is one of scale management required facility as scale formation can be accelerated by temperature. The scale control performance of frequency driver (FD) was tested instead of existing methods such as chemical, physical and electromagnetic methods which needs chemicals and electric power. Three kinds of pipe coupons were submerged in test water with 500 mg/L of hardness for 33 days and XRD and SEM were analysed for comparing scale formation characteristics of these coupons. Calcite ($CaCO_3$) which came from hardness of water was formed on only cast iron pipe coupon and this coupon showed higher corrosion rate than copper and stainless steel pipe coupon. Hot water circulating system connected cast iron pipe with and without FD was operated with 300 mg/L of hardness water at $50^{\circ}C$ for monitoring of scale formation and water quality with and without FD. XRD showed that FD leaded to magnetite ($Fe_3O_4$) scale which is good scale for preventing corrosion than calcite and SEM image also indicated the scale control effect of FD. Scales of 16% on pipe joint, 14% on pipe length, and 42% on heat exchanger decreased with FD comparing scales of those parts without FD. From the results of water quality, FD reduced crystallization of hardness material without chemical reaction in water and it can indicate that FD is safe and proenvironmental technology for scale reduction.

Study on the shaping process of turbocharger nozzle slide joint (터보차저 노즐 슬라이드 조인트의 정형공정에 관한 연구)

  • Kim, Bong-Ju;Lee, Seon-Bong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.1
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    • pp.107-114
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    • 2017
  • A turbocharger is an engine supercharger that is driven by exhaust gas. It improves the output and fuel efficiency by increasing the charging efficiency of the mixture gas, which is achieved by changing the rotatory power of the turbine connected to the exhaust passage. It is important to control the supercharging for this purpose. A nozzle slide joint is one of the core parts. Austenitic stainless steel is currently used as the material for this part, and its excellent mechanical properties include high heat resistance and corrosion resistance. However, because of its poor machinability, there are many difficulties in producing products with complicated shapes. Machining is used in the production of nozzle slide joints for high dimensional accuracy after metal powder injection molding. As design variables in this study, we investigated the sintering temperature, product stress, deformation rate, radius of curvature of the punch, and angle of the chamfer punch, which are related to the strain and shapes. The goal is to suggest a forming process using Nitronic 60 that does not require machining to manufacture a nozzle slide joint for a turbocharger. Accordingly, we determined the best process environment using finite-element analysis, the signal-noise ratio, and the Taguchi method for experiment design. The relative density and hydrostatic pressure of the final product were in accordance with the results of the finite element analysis. Therefore, we conclude that the Taguchi method can be applied to the design process of metal powder injection molding.

Sludge Solubilization by Pre-treatment and its Effect on Methane Production and Sludge Reduction in Anaerobic Digestion (전처리 방법에 따른 슬러지 가용화가 혐기소화에서 메탄 생산과 슬러지 감량에 미치는 영향)

  • Kim, Dong-Jin;Kim, Hye-Young
    • Korean Chemical Engineering Research
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    • v.48 no.1
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    • pp.103-109
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    • 2010
  • Anaerobic digestion has been widely used for the treatment of sludge, which is generated from the municipal and industrial wastewater treatment, for its volume reduction and methane production. Many researches on sludge pre-treatment have been carried out in order to enhance the performance of anaerobic digestion by increasing the hydrolysis of sludge which is the rate limiting step of anaerobic digestion. In this study, the effect of pre-treatment on sludge hydrolysis(solubilization), methane production and sludge reduction by anaerobic digestion after thermal, ultrasonic, and thermal-alkali sludge treatment were compared. Thermal-alkali treatment showed 67 and 70% solubilization with municipal and industrial wastewater sludge, respectively, while ultrasonic treatment and thermal treatment gave similar solubilization efficiency of 40% or more. Methane content of the anaerobic digestion gas reached 45~70% and pretreated sludge gave higher methane content than the control sludge. Methane production of thermal, ultrasonic, and thermal-alkali pre-treatment gave 2.6, 2.7, 3.5 times of municipal control sludge and 3.5, 4.1, 4.2 times of industrial control sludge, respectively. Sludge reduction of pre-treated sludge after anaerobic digestion gave 5~19% point higher than that of control sludge, and thermal-alkali treatment showed higher reduction efficiency than thermal and ultrasonic treatment. The results proved that pre-treatment contributed significantly not only for the methane production but also for the cost reduction of sludge treatment and disposal, and thermal-alkali treatment gave the best performance for the sludge treatment.

Experimental Study on the Designed Ventilation System Performance at Rescue Station in Tunnel Fire (터널 내 화재발생시 구난역 내의 설계된 환기 시스템 성능에 대한 실험적 연구)

  • Kim, Dong-Woon;Lee, Seong-Hyeok;Ryou, Hong-Sun;Yoon, Sung-Wook
    • Journal of the Korean Society for Railway
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    • v.12 no.1
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    • pp.9-15
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    • 2009
  • In this study, the l/35 reduced-scale model experiment were conducted to investigate designed ventilation system performance at rescue station in tunnel fire. A model tunnel with 2 mm thick of steel, 10 m long, 0.19 m high and 0.26m was made by using Froude number scaling law. The cross-passages installing escape door at the center. were connected between accident tunnel and rescue tunnel. The n-heptane pool fire, $4cm\times4cm$, with heat release rate 698.97W were used as fire source. The fire source was located in the center and portal of accident tunnel as Worst case.. An operating ventilation system extracted smoke amount of 0.015 cms. The smoke temperature and carbon monoxide. concentration in cross-passage were measured to verify designed ventilation system. The results showed that, in center fire case without ventilation in accident tunnel, smoke did not propagated to rescue station. In portal tire case, smoke spreaded to rescue station without ventilation. But smoke did not propagated to rescue station with designed ventilation.

Effect of Cure System on the Life-time of Hydrogenated NBR O-ring using Intermittent Compression Stress Relaxation(CSR) (간헐 압축응력 완화를 이용한 가교 구조가 hydrogenated NBR 오링의 수명에 미치는 영향 연구)

  • Lee, Jin-Hyok;Bae, Jong-Woo;Kim, Jung-Su;Hwang, Tae-Jun;Choi, Yu-Seok;Baek, Kwang-Sae;Jo, Nam-Ju
    • Elastomers and Composites
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    • v.46 no.2
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    • pp.144-151
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    • 2011
  • Intermittent CSR testing was used to investigate the degradation of a hydrogenated NBR(HNBR) O-rings, and also the prediction of its life-time. The cure system of HNBR O-ring was controlled as sulfur cure and peroxide cure system. An intermittent CSR jig was designed taking into consideration the O-ring's environment under use. The testing allowed observation of the effects of friction, heat loss, and stress relaxation by the Mullins effect. Degradation of O-rings by thermal aging was observed between 100 and $120^{\circ}C$. In the temperature range of $100-120^{\circ}C$, O-rings showed linear degradation behavior and satisfied the Arrhenius relationship. The activation energy of HNBR-S was about 70.6 kJ/mol. From Arrhenius plots, predicted life-times of HNBR-S O-ring were 31.1 years and 33.7 years for 50% and 40% failure conditions, respectively. In case of HNBR-P, the activation energy was about 72.1kJ/mol, and predicted life-times were 34.0 years and 36.5 years for 50% and 40% failure conditions, respectively. The peroxide cure system showed slower degradation rate and higher activation energy than the sulfur cure system.

Characteristics of Flame Retardent and Mothproof Conservation of Microwave Heated wood (마이크로파 가열 목재의 방염·방충 복합 보존처리 특성)

  • Kim, Chong-Gun;Park, Cheul-Woo;Yoon, Tae-Ho;Lim, Nam-Gi
    • Journal of the Korean Wood Science and Technology
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    • v.41 no.3
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    • pp.234-246
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    • 2013
  • It was found that test piece heated rapidly by 3 kW microwave for 5 minutes satisfies the targeted temperature and the percentage of moisture content, and the highest rate of weight increase is obtained in case of 120 minute immersion in the mixture of phosphates and heterocyclic compounds, from the result of such analysis as: kiln drying schedule, flame retardent by flammability test, insect resistance by termites, and permeability of combined penetrant for the wood after assigning multifunctional finish by immersing conifer structural frame, which is used for the frame work of wooden house and indoor/outdoor finishing in flame retardant and insect repellent materials mixture with the remaining heat of microwave. In addition, after a test of flame retardent treated item, it was identified that every mixture of phosphates corresponds with the standards of flame retardent, and upon investigation of moritality of 7 days after putting termites, it was showed that test piece immersed in the mixture of phosphates and heterocyclic compounds has the best characteristics, showing over 96% of high moritality. From the analysis of inward permeability of combined penetrant for the wood, it was decided that excellent performance in the flame retardent and insect resistance of the wood revealed due to full penetration of combined penetrant as it was found that combined penetrant penetrated through the whole inner cells of the wood.

The Experimental Study of Various Salts on the Characteristics of Heat Transfer in Indirect Moxibustion with Salt (염(鹽)의 종류(種類)에 따른 격염구(隔鹽灸)의 열전달(熱傳達) 특성(特性)에 관한 실험적(實驗的) 연구(硏究))

  • Lee, Geon-mok;Lee, Gun-hyee;Seo, Eun-mi
    • Journal of Acupuncture Research
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    • v.21 no.1
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    • pp.119-135
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    • 2004
  • Objective: The purpose of this study is to investigate the mechanism and effect of moxibustion with salt objectively, to be used as the quantitative data through the measurement of temperature, and to grasp the thermodynamic characteristics of moxibustion with salt. Methods: We have selected of the moxibustion with salt of indirect moxibustion. We make a comparative study of the thermodynamic characteristics of moxibustion with salt as a kind of the 4 salt. We examined combustion times, temperatures, temperature gradients in each period during a combustion of moxa. Results: 1. We make out that it is not significantly change the time of thermal conduction of moxibustion with salt as a kind of 4 salt. 2. The heating period of Refined Salt was long and that of Sun-dried Salt was a short time respectively. The heating period of maximum tamperature is high Sun-dried Salt, Mechanical Salt and Refined Salt orderly. Maximun heating speed in the heating period was $0.151{\sim}0.184^{\circ}C/sec$ and at the same tendency of the maximum temperature in the heating period. 3. The retaining period was shorter than the heating period respectively, that is 15~24% of the combustion time of in the heating period. We make out that it is not significantly different the time of the retaining period as a kind of 4 salt. The mean temperature of retaining period was $43.2{\sim}48.1^{\circ}C$, that was extraordinarily high temperature. 4. We make out that it is not significantly different the time of the cooling period as a kind of 4 salt. The cooling period was measured 223~233sec. Beacuse the same density and size of moxa combustion was made an experiment. 5. The effective combustion time of Refined Salt is longer(259sec) than that of Sun-dried Salt(173sec). It is significantly different the time of the combustion time as a kind of 4 salt. 6. It is significantly different the Sample deviation of the combustion time as a kind of 4 salt because of the water content of the 4 salt individually. Conclusions: As the base on this study, we obtained the conclusion as the follows. The salt of moxibustion with salt was fitted for Sun-dried Salt due to making to Mechanical Salt recently. The Refined Salt is composition rate to another and small size comparatively. So It was fitted for the salt of moxibustion with salt. It is necessary to study continuously about the more suitable moxibustion with salt and quantitative analysis about the moxibustion with salt.

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1-D Analysis for Water Spray Cooling of Exhaust Gas in Combustor Test Facility (물 분무를 이용한 연소가스 냉각 1차원 해석)

  • Im, Ju Hyun;Kim, Myung Ho;Kim, Yeong Ryeon
    • Journal of the Korean Society of Propulsion Engineers
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    • v.19 no.1
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    • pp.61-67
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    • 2015
  • The cooling of hot exhaust gas is an important issue for the construction of combustor test facility. Water spray is an effective method for exhaust gas cooling due to its large latent heat in process of evaporation. In this study, 1-D analysis has been performed based on continuity, energy conservation, and saturated vapor property to understand water spray cooling of combustion gas. In the exhaust duct of combustor test facility, the injected water decreases combustion gas temperature, and evaporates in the combustion gas. However, some of the injected water is collected in the sump due to condensation. The evaporation of water helps combustion gas cooling, but causes pressure increase inside the exhaust duct due to increase of vapor pressure. These phenomena has been analyzed by 1-D modeling in this study. From 1-D analysis, the adequate mass flow rate of water spray to cool combustion gas and to avoid excessive pressure rise inside the exhaust duct has been decided.

Evaluation of Dietary Carbohydrate Sources for Juvenile Abalone (Haliotis discus hannai) (참전복 사료의 탄수화물원 평가)

  • 이상민;윤성종;유성규
    • Journal of Aquaculture
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    • v.11 no.2
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    • pp.133-140
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    • 1998
  • A 20-week growth trial was conducted in flow-through aquarum system to investigate the practical dietary carbohydrate sources for juvenile abalone (Haliotis discus hannai). Four replicate grops of the abalone averaging 0.125g were fed one of eight diets containing 24.2% wheat flour (WF), 20% dextrin (DEX), 20% sucorse (SUC), 10% $^{\alpha}$-potato starch+10% $^{\beta}$-potato starch (ab-S), 15% $^{\alpha}$-potato starch (a-S15), 20% $^{\alpha}$-potato starch (a-S20), 25% $^{\alpha}$-potato starch (a-S25), or mixture (MIX) with practical ingredients such as soybean meal, corn gluten meal, cotton seed meal and heat flour. In addition, these formulated diets were compare with macroalgae such as dried sea mustard Undaria (D-SM) or dried sea tangle Laminaria(D-ST). Survival rate, weight gain, shell growth and soft body weight of abalone were not significantly affected by the different dietary carbohydrate sources (P>0.05), whereas those fed a-S15 diet were slightly low. These values of abalone fed D-ST were lowest (P<0.05), followed by those fed D-SM. Lipid contents of soft body from abalones fed a-S25, D-ST or D-SM were significantly lower than those of abalone fed other diets (P<0.05). These data indicate that abalone can equally utilize any carbohydrate sources used in this study.

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