• Journal of Microbiology and Biotechnology
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• v.29 no.1
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• pp.44-54
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• 2019

Geldanamycin and its derivatives, inhibitors of heat shock protein 90, are considered potent anticancer drugs, although their biosynthetic pathways have not yet been fully elucidated. The key step of conversion of 4,5-dihydrogeldanamycin to geldanamycin was expected to catalyze by a P450 monooxygenase, Gel16. The adequate bioconversions by cytochrome P450 mostly rely upon its interaction with redox partners. Several ferredoxin and ferredoxin reductases are available in the genome of certain organisms, but only a few suitable partners can operate in full efficiency. In this study, we have expressed cytochrome P450 gel16 in Escherichia coli and performed an in vitro assay using 4,5-dihydrogeldanamycin as a substrate. We demonstrated that the in silico method can be applicable for the efficient mining of convenient endogenous redox partners (9 ferredoxins and 6 ferredoxin reductases) against CYP Gel16 from Streptomyces hygroscopicus. The distances for ligand FDX4-FDR6 were found to be $9.384{\AA}$. Similarly, the binding energy between Gel16-FDX4 and FDX4-FDR6 were -611.88 kcal/mol and -834.48 kcal/mol, respectively, suggesting the lowest distance and binding energy rather than other redox partners. These findings suggest that the best redox partners of Gel16 could be NADPH ${\rightarrow}$ FDR6 ${\rightarrow}$ FDX4 ${\rightarrow}$ Gel16.

• Journal of Advanced Marine Engineering and Technology
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• v.40 no.1
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• pp.1-9
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• 2016

Oysters are rich in nutrients with good flavor, and disease prevention is required in both the East and the West for high-quality seafood. The best way to store and transport mass-produced oysters is using dry techniques. Using both hot and frozen drying technologies to obtain a perfectly dried oyster often destroys much of the flavor and nutrients found with the oyster meat. This study uses a low temperature vacuum drying technology to investigate the final weight ratio of wild and farmed dried oysters. Additionally, the heat transfer characteristics of steamed oysters are discussed in this paper.

• Nuclear Engineering and Technology
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• v.43 no.3
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• pp.301-308
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• 2011

The diametral creep of pressure tubes (PTs) in CANDU (CANada Deuterium Uranium) reactors is one of the principal aging mechanisms governing the heat transfer and hydraulic degradation of the heat transport system (HTS). PT diametral creep leads to diametral expansion, which affects the thermal hydraulic characteristics of the coolant channels and the critical heat flux (CHF). The CHF is a major parameter determining the critical channel power (CCP), which is used in the trip setpoint calculations of regional overpower protection (ROP) systems. Therefore, it is essential to predict PT diametral creep in CANDU reactors. PT diametral creep is caused mainly by fast neutron irradiation, temperature and applied stress. The objective of this study was to develop a bundle position-wise linear model (BPLM) to predict PT diametral creep employing previously measured PT diameters and HTS operating conditions. The linear model was optimized using a genetic algorithm and was devised based on a bundle position because it is expected that each bundle position in a PT channel has inherent characteristics. The proposed BPLM for predicting PT diametral creep was confirmed using the operating data of the Wolsung nuclear power plant in Korea. The linear model was able to predict PT diametral creep accurately.

• 한국신재생에너지학회:학술대회논문집
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• 2010.06a
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• pp.105.1-105.1
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• 2010

Korea Electric Power Research Institute (KEPRI) has developed a compact coal-gasification system to accumulate our experiment skills. The combustion furnace for residual oil was modified as a small size coal gasifier. Recently, coal feeding system was also upgraded to control coal feed rate more accurately. Our research group has conducted several experiments to find out the effect of $O_2$/coal ratio on the cold gas efficiency. Furthermore, the effect of $N_2$/coal ratio on the transport characteristics was also studied. According to the calculation of heat and mass balance, the cold gas efficiency was estimated to the maximum at $O_2$/coal ratio of around 0.73. But small size gasifier such as ours required higher value of $O_2$/coal ratio than that of the theoretical estimation. On the optimal $N_2$/coal ratio, we noticed that the coal feed rate was intimately related with the transporting gas pressure and the pipe diameter.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.05b
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• pp.435-440
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• 1996

Large Loss-of-Coolant Accidents analyzed in Final Safety Analysis Reports are usually covered by Reactor Inlet Header. Reactor Outlet Header and Primary Pump Suction breaks as representative cases. In this study we analyze the total (guillotine) break of an Emergency Core Cooling System (ECCS) pipe located at the ECCS injection point into the Primary Heat Transport System (PHTS). It was expected that thermal-hydraulic behaviors in the PHT and ECC systems are different from those of a Reactor Inlet Header break, having an equivalent break size. The main purpose of this study is to get insights on the differences occurred between the two cases and to assess these differences from the phenomenon behavior point of view. It was also investigated whether the ECCS line break analysis results could be covered by header break analysis results. The study reveals that as the intact loop has almost the same behavior in both analyzed cases. broken loop behavior is different mostly regarding sheath temperature in the critical core pass and pressure decrease in the broken Reactor Inlet Header. Differences are also met in the ECCS behavior and in event sequences timings.

• 한국가시화정보학회:학술대회논문집
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• 2002.04a
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• pp.79-84
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• 2002

Most microfluidic devices such as heat sinks for cooling micro-chips, DNA chip, Lab-On-Chip, and micro pumps etc. have microchannels of various size. Therefore, the design of practical microfluidics demands detail information on flow structure inside the microchannels. However, detail velocity field measurements are rare and difficult to carry out. In addition, as the microfluidics expands, accurate understanding of microscale transport phenomena becomes very important. In this research, micro-PIV system was employed to measure the velocity fields of flow inside a micro-channel. We carried out PIV measurements for several microchannels with varying channels width, inlet and outlet shape, filters, CCD camera and ICCD camera, etc. For effective composition of micro-PIV system, first of all, it is essential to understand optics related with micro-imaging of particles and the particle dynamics encountered in micro-scale channel flows. In addition, it is necessary to find the optimal condition for given experimental environment and? micro-scale flow to be investigated. The problems encountered in measuring velocity field of micro-channel flows are discussed in this paper.

• Journal of Power System Engineering
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• v.13 no.5
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• pp.25-33
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• 2009

In the present study, the numerical investigation has been carried out to see the effects of water mist sprays on the fire suppression mechanism. The special-purposed program named as FDS was used to simulate the interaction of fire plume and water mists. This program solves the fire-driven flows using LES turbulence model, the mixture fraction combustion model, the finite volume method of radiation transport for a non-scattering gray gas, and conjugate heat transfer between wall and gas flow. The computational domain was composed of a rectangular space dimensioned as $L{\times}W{\times}H=4.0{\times}4.0{\times}2.5\;m^3$ with a mist-injecting nozzle installed 1.0 m high from the fire pool. In this paper, two types of nozzles were chosen to compare the performance of the fire suppression. Numerical results showed that the nozzle, type A, with more orifices having smaller diameters had poorer performance than the other one, type B because the flow injected through side holes deteriorated the primary flow. The fire-extinguishing time of type A was 2.6 times bigger than that of type B.

• Journal of Magnetics
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• v.10 no.2
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• pp.66-70
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• 2005

The junction properties between the ferromagnet (FM) and two-dimensional electron gas (2DEG) system are crucial to develop spin electronic devices. Two types of 2DEG layer, InAs and GaAs channel heterostructures, are fabricated to compare the junction properties of the two systems. InAs-based 2DEG layer with low trans-mission barrier contacts FM and shows ohmic behavior. GaAs-based 2DEG layer with $Al_2O_3$ tunneling layer is also prepared. During heat treatment at the furnace, arsenic gas was evaporated and top AlAs layer was converted to aluminum oxide layer. This new method of forming spin injection barrier on 2DEG system is very efficient to obtain tunneling behavior. In the potentiometric measurement, spin-orbit coupling of 2DEG layer is observed in the interface between FM and InAs channel 2DEG layers, which proves the efficient junction property of spin injection barrier.

• Nuclear Engineering and Technology
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• v.45 no.5
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• pp.581-588
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• 2013

This paper deals with the Safety Analysis for $CANDU^{(R)}$ 6 nuclear reactors as affected by main Heat Transport System (HTS) aging. Operational and aging related changes of the HTS throughout its lifetime may lead to restrictions in certain safety system settings and hence some restriction in performance under certain conditions. A step in confirming safe reactor operation is the tracking of relevant data and their corresponding interpretation by the use of appropriate thermal-hydraulic analytic models. Safety analyses ranging from the assessment of safety limits associated with the prevention of intermittent fuel sheath dryout for a slow Loss of Regulation (LOR) analysis and fission gas release after a fuel failure are summarized. Specifically for fission gas release, the thermal-hydraulic analysis for a fresh core and an 11 Effective Full Power Years (EFPY) aged core was summarized, leading to the most severe stagnation break sizes for the inlet feeder break and the channel failure time. Associated coolant conditions provide the input data for fuel analyses. Based on the thermal-hydraulic data, the fission product inventory under normal operating conditions may be calculated for both fresh and aged cores, and the fission gas release may be evaluated during the transient. This analysis plays a major role in determining possible radiation doses to the public after postulated accidents have occurred.

• International Journal of Air-Conditioning and Refrigeration
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• v.6
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• pp.56-66
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• 1998

The absorption process of water vapor in a liquid film is an important process in LiBr-Water absorption system. The composition of the gas phase, in which a non-absorbable gas is combined with the absorbate, influences the transport characteristics. In the present work, the absorption processes of water vapor into aqueous solutions of lithium bromide in the presence of non-absorbable gas are investigated. The continuity, momentum, energy and diffusion equations for the solution film and gas are formulated in integral forms and solved numerically. It is found that the mass transfer resistance in gas phase increases with the concentration of non-absorbable gas. However the primary resistance to mass transfer is in the liquid phase. As the concentration of non-absorbable gas in the absorbate increases, the interfacial temperature and concentration of absorbate in solution decrease, which results in the reduction of absorption rate. The reduction of mass transfer rate is found to be significant for the addition of a small amount of non-absorbable gas to the pure vapor, especially at the outlet of tube where the non-absorbable gas accumulates. At higher non-absorbable gas concentration, the decrease of absorption rate seems to be linear to the concentration of non-absorbable gas.