• 대한물리의학회지
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• 제14권2호
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• pp.71-77
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• 2019

Purpose: The purpose of this study was to compare the level of muscle activation on the cervical erector spinae (CES), lumbar erector spinae (LES), and upper trapezius (UT) according to the sitting postures while using a smartphone in the bathroom. Method: Thirty-two healthy subjects were recruited for this study. The CES, LES, and UT were evaluated by surface electromyography according to two sitting postures while using a smartphone on the toilet seat. A paired t test was performed for the root mean square of reference voluntary contraction (%RVC) comparisons between two sitting postures, and one-way ANOVA was used to compare the three muscle activations within each posture. Results: A comparison between the two sitting postures revealed the muscle activation of both CESs in sitting posture 2 and both LESs in sitting posture 1 to be significantly higher than those of the others. In sitting posture 1, the muscle activation of both LESs was significantly higher than those of the CES and UT. In sitting posture 2, the muscle activation of both CESs was significantly higher than those of the LES and UT. Conclusion: High muscle activation of the CES and LES was observed according to the sitting postures when using smartphone in the restroom. Therefore, long time use of smartphones on a toilet seat should be avoided.

• Journal of Korean Biological Nursing Science
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• 제23권1호
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• pp.22-30
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• 2021

Purpose: This descriptive study aimed to investigate heart rate variability (HRV) according to sex and to elucidate the influence of negative emotion such as levels of stress, anxiety and depression on HRV among Korean college students based on a neurovisceral integration model. Methods: A descriptive study design was used. Eighty-six healthy college students participated in the study. Resting HRV and standing HRV on orthostatic stimulation were measured for 5 minutes during 4-6 p.m. in the afternoon. Levels of stress, anxiety and depression were assessed using the Global assessment of recent stress, State-Trait Anxiety Inventory and Beck Depression Index, respectively. Results: Out of the 86 students, 47 (54.7%) were men and 39 (45.3%) were women. Root mean square of the differences between adjacent RR intervals (RMSSD) and normalized high frequency (nHF) on standing HRV were significantly lower in men than in women (p= .005, p= .019, respectively). Male gender (β= 0.30, p= .013), higher level of stress (β= -0.36, p= .009) and lower level of depression (β = 0.30, p= .044) exerted a significant influence on decreased nHF in the multiple regression analysis. Conclusion: We suggest that men are more vulnerable to having reduced vagal activity on HRV than women. Since male gender, higher level of stress and lower level of depression level influenced decreased vagal activity, strategies are needed to improve stress and depression rather than anxiety especially for men, which contribute to promoting HRV to prevent cardiac health diseases.

• 한방재활의학과학회지
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• 제32권2호
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• pp.139-154
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• 2022

Objectives This study was conducted to confirm anatomical information near the treatment areas of visceral chuna manual therapy and to secure stability and effectiveness during the treatment. Methods For 50 healthy adult men and women, a total of 13 ultrasound images were taken of the 7 treatment areas which are the representative treatment areas of visceral Chuna manual therapy. Results The treatment area of the bottom of the liver can be palpated around the right side ST19 and CV14. The treatment area of the gallbladder and the common bile duct can be palpated around the right side SP16 and ST20. The treatment area of the cardia and the pylorus can be palpated around the left side KI21, right side ST20, ST21, KI19, KI20, CV12, and CV13. The origin point of the mesentery root can be moved to the left and lower left from CV12 and can be palpated. The treatment area of the ileocecal valve and the cecum can be palpated around the right side SP14. The treatment area for the colic flexure can be palpated around the both side LR13. The treatment support point for the kidneys can be palpated around both side BL51. Conclusions It is thought that if the ultrasound image data from the meridians around the treatment site is used as an auxiliary, it will be more effective in terms of safety and effectiveness during the treatment of visceral Chuna manual therapy.

• 여성건강간호학회지
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• 제28권3호
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• pp.210-221
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• 2022

Purpose: The purpose of this study was to construct a hypothetical model based on Meleis and colleagues' Transition Theory and a literature review to explain women's menopausal transition, constructing a modified model considering previous studies and model fit and testing the effects between variables. Methods: With a correlational survey design, middle-aged Korean women aged 40 to 64 years who had experienced menopausal symptoms were recruited and filled out a self-administered study questionnaire. Measures included menopausal symptoms, resilience, social support, menopause management, menopause adaptation, and quality of life. The data were analyzed using SPSS 24.0 and AMOS 24.0. Results: The model fit indices were considered acceptable: 𝛘²/degree of freedom=2.93, standardized root mean residual=.07, comparative fit index=.90, and parsimonious normed fit index=.73. All eight direct-effect paths-from menopausal symptoms to support and adaptation, from support to adaptation and resilience, from resilience to adaptation and management, from management to quality of life, and from adaptation to quality of life-were significant. The explanatory power of the menopause transition model was 63.6%. Conclusion: Women who experience menopausal symptoms may be able to maintain and improve their quality of life if menopause management and menopause adaptation are successful through resilience and social support. Future research is needed to confirm whether strengthening facilitation as a nursing intervention strategy may promote healthy response patterns.

• Physical Therapy Rehabilitation Science
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• 제11권3호
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• pp.311-320
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• 2022

Objective: To investigate the reliability and validity of static balance measurements using an acceleration sensor and a gyroscope sensor in smart phone inertial sensors. Design: Equivalent control group pretest-posttest. Methods: Subjects were forty five healthy adults aged twenty to fifty-years-old who had no disease that could affect the experiment. After pre-test, all participants wore a waist band with smart phone, and conducted six static balance measurements on the force plate twice for 35 seconds each. To investigate the test-retest reliability of both smart phone inertial sensors, we compared the intra-correlation coefficient (ICC 3, 1) between primary and secondary measurements with the calculated root mean scale-total data. To determine the validity of the two sensors, it was measured simultaneously with force plate, and the comparision was done by Pearson's correlation. Results: The test-retest reliability showed excellent correlation for acceleration sensor, and it also showed excellent to good correlation for gyroscope sensor(p<0.05). The concurrent validity of smartphone inertial sensors showed a mostly poor to fair correlation for tandem-stance and one-leg-stance (p<0.05) and unacceptable correlation for the other postures (p>0.05). The gyroscope sensor showed a fair correlation for most of the RMS-Total data, and the other data also showed poor to fair correlation (p<0.05). Conclusions: The result indicates that both acceleration sensor and gyroscope sensor has good reliability, and that compared to force plate, acceleration sensor has unacceptable or poor correlation, and gyroscope sensor has mostly fair correlation.

• Journal of Veterinary Science
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• 제23권3호
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• pp.50.1-50.12
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• 2022

Background: There is an urgent need to find reliable and rapid bovine tuberculosis (bTB) diagnostics in response to the rising prevalence of bTB worldwide. Toll-like receptor 2 (TLR2) recognizes components of bTB and initiates antigen-presenting cells to mediate humoral immunity. Evaluating the affinity of antigens with TLR2 can form the basis of a new method for the diagnosis of bTB based on humoral immunity. Objectives: To develop a reliable and rapid strategy to improve diagnostic tools for bTB. Methods: In this study, we expressed and purified the sixteen bTB-specific recombinant proteins in Escherichia coli. The two antigenic proteins, MPT70 and MPT83, which were most valuable for serological diagnosis of bTB were screened. Molecular docking technology was used to analyze the affinity of MPT70, MPT83, dominant epitope peptide of MPT70 (M1), and dominant epitope peptide MPT83 (M2) with TLR2, combined with the detection results of enzyme-linked immunosorbent assay to evaluate the molecular docking effect. Results: The results showed that interaction surface Cα-atom root mean square deviation of proteins (M1, M2, MPT70, MPT83)-TLR2 protein are less than 2.5 A, showing a high affinity. It is verified by clinical serum samples that MPT70, MPT83, MPT70-MPT83 showed good diagnostic potential for the detection of anti-bTB IgG and M1, M2 can replace the whole protein as the detection antigen. Conclusions: Molecular docking to evaluate the affinity of bTB protein and TLR2 combined with ELISA provides new insights for the diagnosis of bTB.

• 한국환경과학회지
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• 제32권1호
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• pp.77-88
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• 2023

Kadsura coccinea (Lem.) A.C. Smith is used as a medicinal plant and cosmetic material in China and Southeast Asia. To mass-produce Kadsura coccinea seedlings, the effects of gibberellic acid (GA₃) and cold stratification treatments on seed germination were investigated. Seed germination rate with GA₃ treatment was most effective at concentrations of 250 or 500 mg/L. With respect to mean germination time (MGT), mean daily germination, and T₅₀ (days to reach 50% seed germination), the germination-promoting effect was improved as the concentration of GA₃ increased. Stem growth of seedlings was the highest following GA₃ treatments of 250 and 500 mg/L, and the growth promoting effect gradually decreased as the concentration of GA₃ decreased. Root growth was stimulated at GA₃ concentrations of 250-1,000 mg/L. Examination of the effect of stratification treatment for 15, 30 and 60 days at temperatures of 0, 5 and 10℃ on the germination rate revealed that the most stratification treatment temperature was 10℃, and the results improved with longer treatment periods. Altogether, GA₃ and stratification treatments improved the seed germination rate, shortened the MGT, improved germination uniformity, and produced healthy seedlings.

• Korean Journal of Acupuncture
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• 제39권4호
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• pp.132-141
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• 2022

Objectives : Some acupoints are commonly utilized to treat a variety of diseases. The acupoints appear to have a wide range of effects caused by several mechanisms. The purpose of this study is to investigate into the potential role of microRNAs (miRNAs) in the multipotent effects of individual acupoint stimulation. Methods : We examined the miRNA expressions in the dorsal root ganglia (DRG) of neuropathic or inflammatory pain rats following ST36 and GB34 electroacupuncture (EA) stimulation. Neuropathic pain was induced by L5 spinal nerve ligation. Inflammatory pain was induced by knee joint injection of Complete Freund's adjuvant (CFA). EA was given under gaseous anesthesia with the same parameters (1mA, 2Hz, 30 min) in 5 consecutive days. Pain behaviors and miRNA expressions were analyzed. Results : In rats with neuropathic and inflammatory pain, EA treatments significantly enhanced the paw withdrawal threshold and weight-bearing force. After nerve injury, 36 miRNAs were upregulated in the DRG of neuropathic rats, while EA downregulated 10 of them. Furthermore, 14 miRNAs were downregulated following nerve damage, while one was increased by EA. 15 miRNAs were increased in the DRG of inflammatory rats following CFA injection, while 5 were downregulated by EA. Furthermore, 17 miRNAs were downregulated following CFA injection, while 7 were increased by EA. The miRNAs rno-miR-335, rno-miR-381-5p, rno-miR-1306-3p, and rno-miR-1839-3p were regulated by EA in both models. Conclusions : In two pain models, EA applied to ST36 and GB34 regulated miRNA expression differently. There appeared to be both acupoint-specific and non-specific miRNAs, and miRNA regulation of differential protein expression may modulate a variety of EA mechanisms.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.19-19
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• 2020

The Cassava(Manihot esculenta Crantz) is a tropical root crop, originally from Amazonia, that provides the staple food of an estimated 800 million people worldwide. It belongs to the family Euphorbiaceae which also includes rubber (Hevea brasiliensis) and castor bean (Ricinus communis). Among tropical crops, rice, sugarcane, maize and cassava are the most important sources of calories for human consumption. Problems in the propagation of cassava are virus diseases and low rates of seed germination. So we tried to optimize protocols for mass production of somatic embryo amenable to large-scale vegetative propagation of Cassava. After in vitro eight-week culture of leaves of Cassava, the medium which contained the 2,4-D, BAP and IBA showed the highest callus induction rate, embryogenesis callus formation rate and somatic embryo formation in Cassava culture. In the medium with GA₃ and myo-inositol, shoots were most vigorously regenerated from somatic embryos of Cassava. Our experiments confirmed that in vitro growth and multiplication of plantlets could depend on its reaction to the different medium composition, and this micropropagation techniques could be a useful system for healthy and vigorous plant production.

• 한방재활의학과학회지
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• 제33권4호
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• pp.167-184
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• 2023

Objectives This study was designed to assess the effectiveness of ultrasound therapy at the ST11 for regulation of sympathetic hyperactivity. Methods Forty healthy adult subjects were assigned to experimental group and control group. After taking mental stress, ultrasound therapy was applied at the ST11 in experimental group and sham-ultrasound therapy was applied in control group. The evaluation of sympathetic activity was measured by blood pressure, pulse rate, and heart rate variability at 3 times (Time 1: before the stress stimulation, Time 2: after the stress stimulation, Time 3: after the intervention). The primary end point was consisted of normalized (norm) low frequency (LF)/high frequency (HF) ratio, LF (norm), HF (norm). The secondary end point was consisted of systolic blood pressure, diastolic blood pressure, pulse rate, mean heart rate, standard deviation of NN intervals, root mean square of the successive differences, total power (log). Results After the stress stimulation, all subjects showed sympathetic hyperactivity. After the intervention, the experimental group showed lower sympathetic activity than the control group. Comparing the Time 3 and Time 1, the experimental group showed no significantly differences in sympathetic activity while the control group showed higher sympathetic activity in Time 3 than Time 1. Comparing the Time 3 and Time 2, the experimental group showed lower sympathetic activity in Time 3 than Time 2 while the control group showed higher sympathetic activity in Time 3 than Time 2. Conclusions We suggest that the ultrasound therapy at ST11 can decrease sympathetic activity in sympathetic hyperactivity condition.