• Title/Summary/Keyword: Health research and development

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Estimating genetic diversity and population structure of 22 chicken breeds in Asia using microsatellite markers

  • Roh, Hee-Jong;Kim, Seung-Chang;Cho, Chang-Yeon;Lee, Jinwook;Jeon, Dayeon;Kim, Dong-kyo;Kim, Kwan-Woo;Afrin, Fahmida;Ko, Yeoung-Gyu;Lee, Jun-Heon;Batsaikhan, Solongo;Susanti, Triana;Hegay, Sergey;Kongvongxay, Siton;Gorkhali, Neena Amatya;Thi, Lan Anh Nguyen;Thao, Trinh Thi Thu;Manikku, Lakmalie
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.12
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    • pp.1896-1904
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    • 2020
  • Objective: Estimating the genetic diversity and structures, both within and among chicken breeds, is critical for the identification and conservation of valuable genetic resources. In chickens, microsatellite (MS) marker polymorphisms have previously been widely used to evaluate these distinctions. Our objective was to analyze the genetic diversity and relationships among 22 chicken breeds in Asia based on allelic frequencies. Methods: We used 469 genomic DNA samples from 22 chicken breeds from eight Asian countries (South Korea, KNG, KNB, KNR, KNW, KNY, KNO; Laos, LYO, LCH, LBB, LOU; Indonesia, INK, INS, ING; Vietnam, VTN, VNH; Mongolia, MGN; Kyrgyzstan, KGPS; Nepal, NPS; Sri Lanka, SBC) and three imported breeds (RIR, Rhode Island Red; WLG, White Leghorn; CON, Cornish). Their genetic diversity and phylogenetic relationships were analyzed using 20 MS markers. Results: In total, 193 alleles were observed across all 20 MS markers, and the number of alleles ranged from 3 (MCW0103) to 20 (LEI0192) with a mean of 9.7 overall. The NPS breed had the highest expected heterozygosity (Hexp, 0.718±0.027) and polymorphism information content (PIC, 0.663±0.030). Additionally, the observed heterozygosity (Hobs) was highest in LCH (0.690±0.039), whereas WLG showed the lowest Hexp (0.372±0.055), Hobs (0.384±0.019), and PIC (0.325±0.049). Nei's DA genetic distance was the closest between VTN and VNH (0.086), and farthest between KNG and MGN (0.503). Principal coordinate analysis showed similar results to the phylogenetic analysis, and three axes explained 56.2% of the variance (axis 1, 19.17%; 2, 18.92%; 3, 18.11%). STRUCTURE analysis revealed that the 22 chicken breeds should be divided into 20 clusters, based on the highest ΔK value (46.92). Conclusion: This study provides a basis for future genetic variation studies and the development of conservation strategies for 22 chicken breeds in Asia.

Development of a hybrid regionalization model for estimation of hydrological model parameters for ungauged watersheds (미계측유역의 수문모형 매개변수 추정을 위한 하이브리드 지역화모형의 개발)

  • Kim, Youngil;Seo, Seung Beom;Kim, Young-Oh
    • Journal of Korea Water Resources Association
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    • v.51 no.8
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    • pp.677-686
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    • 2018
  • There remain numerous ungauged watersheds in Korea owing to limited spatial and temporal streamflow data with which to estimate hydrological model parameters. To deal with this problem, various regionalization approaches have been proposed over the last several decades. However, the results of the regionalization models differ according to climatic conditions and regional physical characteristics, and the results of the regionalization models in previous studies are generally inconclusive. Thus, to improve the performance of the regionalization methods, this study attaches hydrological model parameters obtained using a spatial proximity model to the explanatory variables of a regional regression model and defines it as a hybrid regionalization model (hybrid model). The performance results of the hybrid model are compared with those of existing methods for 37 test watersheds in South Korea. The GR4J model parameters in the gauged watersheds are estimated using a shuffled complex evolution algorithm. The variation inflation factor is used to consider the multicollinearity of watershed characteristics, and then stepwise regression is performed to select the optimum explanatory variables for the regression model. Analysis of the results reveals that the highest modeling accuracy is achieved using the hybrid model on RMSE overall the test watersheds. Consequently, it can be concluded that the hybrid model can be used as an alternative approach for modeling ungauged watersheds.

Effects of various concentrations of skullcap extract in the diets on disease resistance of olive flounder, Paralichthys olivaceus (생약재 황금 뿌리 열수추출물의 넙치 투여시 질병저항성에 미치는 영향)

  • Jee, Bo-Young;Seo, Jung-Soo;Jeon, Eun-Ji;Lee, Eun-Hye;Choi, Hee-Jung;Kim, Jin-Do;Jung, Sung-Hee;Park, Myoung-Ae
    • Journal of fish pathology
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    • v.25 no.1
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    • pp.21-30
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    • 2012
  • Effects of various concentration of skullcap Scutellaria baicalensis in the diets on a nonspecific immunity and a disease resistance of olive flounder were investigated. After feeding trial, weight gain of fish fed 0.05% skullcap immersed group was higher than that of fish fed 0, 0.1 and 1% skullcap diet but no significant differences were observed among the experimental groups. Furthermore, no significant differences in hematological indices of olive flounder were found among the experimental groups. Lysozyme activity in the serum and kidney of the administrated group(0.05% skullcap immersed group) was significantly higher than the control group. In addition, the chemiluminescent(CL) responses of head kidney leucocytes from the 0.05% skullcap immersed group was significantly higher(P<0.05) than the control group. In the histological results, the 1% skullcap immersed group appeared to have the detrimental effects for fish health. In a challenge experiment with Edwardsiella tarda(GY-01) and Streptococcus iniae(FT5228), relative percent survival (RPS) in the 0.05% skullcap immersed group was higher than the control group injected with E. tarda(GY-01) at $4^{th}$ and $8^{th}$ weeks. The results suggest that the skullcap extract (0.05%) would be effective to enhance the nonspecific immunity and protective ability of olive flounder against fish pathogen such as E. tarda.

Protective Effects of Ukgan-san in $CoCl_2$-induced Cell Death of C6 Glial Cells ($CoCl_2$로 유도된 C6 신경교세포의 사멸에 대한 억간산(抑肝散)의 보호 효과)

  • Cho, Mun-Young;Shin, Yong-Jeen;Ha, Ye-Jin;Woo, Chan;Kim, Ta-Jung;You, Ju-Yeon;Choi, Yong-Seok;Choi, Jung-Hoon;Shin, Sun-Ho
    • The Journal of Internal Korean Medicine
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    • v.34 no.2
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    • pp.178-191
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    • 2013
  • Objectives : In this study, we made an effort to investigate the protective mechanism of Ukgan-san (UGS) extracts on hypoxia-induced C6 glial cell death. Methods : The cell viability was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MMT) assay and cell morphological changes were analysed with microscope after staining with crystal violet (CV). Reactive oxygen species (ROS) formation was assessed by flow cytometer after staining with 2'7'-dichlorofluorescein diacetate (DCF-DA). We also analyzed expression of hypoxia-inducible factor-1 alpha (HIF-$1{\alpha}$) and p53, processing of procaspase-3 and procyclic acidic repetitive protein (PARP) by western blot method. Results : We estimated the elevated cell viability by UGS extract on $CoCl_2$-induced C6 glial cells. UGS attenuated $CoCl_2$-induced ROS formation in C6 glial cells and also showed a protective activity compared to antioxidants and exhibited abrogation of LDH-released by $CoCl_2$. UGS suppressed the typical apoptotic cell death markers, caspase-3 and PARP activation. UGS inhibited $CoCl_2$-induced HIF-1${\alpha}$ expression which is known as a major regulator for hypoxia-induced cell death, and suppressed p53 expression. Conclusions : These results suggest that UGS extract contains protective constituents for hypoxia-induced C6 glial cell death.

Application of professor·learning model customized for flipped learning for enhancing basic ability of work - Focused on freshman students in radiology department of specialized colleges (직업기초능력함양을 위한 맞춤식 플립드 러닝 교수·학습모형 적용-전문대학 방사선과 1학년 재학생을 중심으로)

  • Park, Jeongkyu
    • Journal of the Korean Society of Radiology
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    • v.12 no.2
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    • pp.225-231
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    • 2018
  • Recently, new teaching methods for communicating with teachers and students have been emerged according to the trends of decreasing the school-age population and the development of the mass media. We have applied teaching-learning model based on the flip learning to the college students in this work. As a result of the test for the customized flipped learning teaching-learning model in pre-class, the attendance rate of the major subject was 92.3% whereas that in liberal arts courses other than majors revealed 87.6%. This result for attendance rate shows that first year students in the radiology department have been actively participated in pre-class of the major subject than that of the liberal arts curriculum. From comparing the differences between the study group that was applied flipped learning in class and the non-applied group, the research group showed higher scores in knowledge, skills, and attitudes than the comparative group. In addition, more than 90% of the learners improved their responsibility, problem solving ability, creative thinking, cooperative ability, and communication ability through this learning program. From the test for the difference in the role of radiologists in the post class, the mean score was 4.40 for the group applied the teaching-learning model while that for non-applied group was 2.10. Hence, from such results, we see that this teaching-learning model is appropriate and needs to be extended to cultivate basic skills in radiology and relevant vocational education.

The Effects of Mentoring Program by Professer on Adaptation to Campus Life and Satisfaction for Students (방사선과 교수의 멘토링 프로그램이 학생들의 대학생활적응과 만족도에 미치는 영향)

  • Jeong, Che-Rin;Moon, Il-Bong;Kim, Young-Keun;Choi, Seong-Woo
    • The Journal of the Korea Contents Association
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    • v.14 no.9
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    • pp.613-621
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    • 2014
  • This study conducted a questionnaire survey with 362 students at department of radiology of college of public health located at G city, who had participated in mentoring program from Mar. 5 to Jun. 21, 2013. Out of 362 questionnaire copies, 315 copies were collected. The students' satisfaction with campus life was significantly affected by whether or not they joined a club, the number of participation into mentoring program per semester, reliability of mentors and mentoring functions. According to the study result, More specifically in terms of the mentoring functions(p<0.001), career development function(p=0.002) and tailored human-resource raising function(p<0.001) gave statistically significant effects on the satisfaction. It is considered that the study results will be used as a fundamental material to research mentoring for college and university students, and will be implemental in using and developing mentoring program as a direct way of preventing the student's dropout. Also it is expected that mentoring program helps college and university students in the transitional period to adulthood experience mentoring before they work at society, therefore playing a positive role for their successful work life as they get employed.

A Meta-analysis of The Factors Related to Bullying Damage Experience of Adolescent (청소년의 집단따돌림 피해경험과 관련된 요인에 대한 메타분석)

  • Moon, Dong-Kyu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.1
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    • pp.685-697
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    • 2020
  • This study conducts meta-analysis of the effect size of related factors using research papers published within the past 20 years in relation to adolescent bullying experience in Korea. The verification results are as follows. First, in inducing factors, the overall effect size, personal psychology factor, and school life factor group showed a medium effect size. In the causative factors, the aggression, anxiety, stress, depression, suicidal thoughts, and school stress of school psychological factors showed a moderate effect size. Second, in terms of deterrent, the overall effect size, family environment factor, personal psychology factor, and school life factor group showed a medium effect size. In the sub-factors of determinants, the parenting attitude, parental support, and self-esteem and self-resilience of the family environmental factor group also showed a medium effect size. In conclusion, this study is meaningful in that it tried to quantitatively integrate the previous studies to examine objective results. It is meaningful that it provided basic data in program development and policy making to prevent and reduce the bullying of youth.

A Study on the Preference Program of Forest Healing by Region According to Moving Line (이동동선에 따른 지역별 산림치유 선호프로그램에 관한 연구 - 전주시 건지산을 중심으로 -)

  • So, Eun-joo;Park, Yool-jin
    • The Journal of the Korean Institute of Forest Recreation
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    • v.22 no.4
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    • pp.35-48
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    • 2018
  • This research was carried out to provide a good quality forest healing program as a basic source of development by researching the general characteristics of users of Geiji Mountain in North Jeolla Province, the recognition of forest healing and regional preference programs. As a result of the survey, Geigisan Mountain is used as a natural place for recreation and leisure of residents of Jeonju. The most important factors in the selection of forest healing programs for visitors were the activities, organization and chronology of the program, and the main purpose was to improve health. Week-by-week programs utilizing weekends were preferred for management of forest healing programs. It was revealed that sports programs prefer to enjoy natural scenery, forest experience programs, healing programs, forest therapy programs, meditation programs, and listening to forest music. The outcome of this study is expected to be used as a basic source for developing and researching a program for forest healing in the area of Mt. Meanwhile, the limit on the composition of suitable forest healing programs according to preference is required for continuous and continuous integrated study of the forest healing program for the future of the forest healing program.

The Relationship Between Gratitude Disposition and Psychological Well-BeingofUniversity Students: TheMediation Effect of Self-esteem (대학생의 감사성향과 심리적 안녕감의 관계 :자아존중감의 매개효과)

  • Choi, Jung-Myung;Kim, Sun-Mee
    • Journal of the Health Care and Life Science
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    • v.8 no.2
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    • pp.173-179
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    • 2020
  • The purpose of this study is to verify whether the relationship between gratitude disposition and psychological well-being of college students shows a mediating effect of self-esteem The final 211 copies of the data were used in the analysis for the study, which was collected as students attending the university located in Group C. The data were statistically processed using SPSS 21.0 and the hierarchical regression analysis was performed to analyze the collected data. The results of the study are summarized as follows: Looking at the impact of the first-phase gratitude disposition on self-esteem, it was found that gratitude disposition was significant to self-esteem. Looking at the impact of the second-phase gratitude disposition on psychological well-being, gratitude disposition was significant to psychological well-being. Since the three-stage gratitude disposition was significant to psychological well-being, self-esteem can be seen to have partial medias. The results of this study confirmed that gratitude disposition was necessary as psychological resources to promote psychological well-being of university students, and self-esteem had a mediating effect. It is hoped that this research result will be used as a basis for the development of programs to improve gratitude disposition and self-esteem in order to promote the psychological well-being of college students.

A Study on the Analysis of Virus Barrier Materials in a Chest X-ray Laboratory to Respiratory and Droplet Infections Only Patients (호흡기 및 비말감염 환자 전용 흉부 X-선 검사실의 바이러스 차단제 분석에 관한 연구)

  • Kim, Hyeon-Ju;Lee, Jun-Ho;Choi, Kwan-Yong
    • Journal of the Korean Society of Radiology
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    • v.16 no.2
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    • pp.169-175
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    • 2022
  • In this study, envisioned a laboratory equipped with virus blocking equipment for chest X-ray examinations of respiratory or droplet-transmitted virus-infected patients, and the material with the least deterioration in X-ray output and image quality among the proven blocking materials that block viruses in the design process. and experimented to find the thickness. As a result, when 1 cm of acrylic was applied, the X-ray output was reduced by only about 3.27 % compared to the absence of the barrier material, the SNR was 40.7 and CNR was 30.9, which was the best. The SSIM index result was analyzed as 0.891, which was analyzed to be implemented as the most similar image compared to the original image. The barrier material applied in the research method was objective in that it used a product approved by the Ministry of Food and Drug Safety. the results of this study are expected to provide useful information when installing X-ray examination facilities for the diagnosis and treatment of respiratory-related virus-infected patients in the future.