• 한국축산식품학회지
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• 제30권4호
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• pp.590-596
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• 2010

Meat and meat products are a potential source of food-borne pathogens, including Staphylococcus aureus, Salmonella spp., Escherichia coli O157:H7, and Bacillus cereus. A sensitive and specific PCR assay for the detection of these pathogens in meat and meat products was developed in this study, as part of a broader effort to reduce the potential health hazards posed by these pathogens. Initially, PCR conditions were standardized with purified DNA. Under standard conditions, the detection level for PCR was as low as 10 pg of purified bacterial DNA. After overnight growth of bacteria in a broth medium, as few as $10^2$ CFU of bacteria were detected by PCR assay. The primers employed in the PCR assay were found to be highly specific for individual organisms, and evidenced no cross-reactivity with heterologous organisms. Additionally, the multiplex PCR assays also amplified some target genes from the four pathogens, and multiplex amplification was obtained from as little as 10 pg of DNA, thus illustrating the excellent specificity and high sensitivity of the assay. In conclusion, this PCR-based technique provides a sensitive and specific method for the detection of S. aureus, Salmonella spp., E. coli O157:H7, and B. cereus in meat and meat products.

• 한국게임학회 논문지
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• 제16권6호
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• pp.101-110
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• 2016

현재 미세먼지에 대한 화재가 점점 사람들의 시선을 끌고 있다. 미세먼지는 눈으로 볼 수 없는 직경이 $2.5{\mu}m$(마이크로미터)인 먼지이며 폐 속 깊숙이 침투 할 수 있어 건강에 많은 피해를 준다. 사람들은 예방법을 알면서도 실행에 옮기지 않는 것은 미세먼지 위험성의 인지도가 낮기 때문이다. 본 논문은 어린이 대상으로 미세먼지의 인위적 원인과 자연적 원인의 생성 요소와 미세먼지 예방요소에 대한 인지도를 향상시키기 위하여 <우마이 탈출>보드게임 개발하여 다수의 어린에게 테스트를 진행하여 <우마이 탈출>보드게임의 교육적 효과에 대한 연구를 진행하였다.

• Journal of Mechanical Science and Technology
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• 제18권12호
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• pp.2182-2189
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• 2004

In the recent years, environmentally conscious design and manufacturing technologies have attracted considerable attention. The coolants, lubricants, solvents, metallic chips and discarded tools from manufacturing operations will harm our environment and the earth's ecosystem. In the present work, the Tukey method of multiple comparisons is used to select the minimum level of coolant required in a turning process. The amount of coolant is varied in 270 designed experiments and the parameters cutting temperature, surface roughness, and specific cutting energy are carefully evaluated. The effects of coolant mix ratio as well as the amount of coolant on the turning process are studied in the present work. The cutting temperature and surface roughness for different quantity of coolant are investigated by analysis of variance (ANOVA) - test and a multiple comparison method. ANOVA-test results signify that the average tool temperature and surface roughness depend on the amount of coolant. Based on Tukey's Honestly Significant Difference (HSD) method, one of the multiple comparison methods, the minimum level of coolant is 1.0 L/min with 2% mix ratio in the aspect of controlling tool temperature. F-test concludes that the amount of coolant used does not have any significant effect on specific cutting energy. Finally, Tukey method ascertains that 0.5 L/min with 6% mix ratio is the minimum level of coolant required in turning process without any serious degradation of the surface finish. Considering all aspects of cutting, the minimum coolant required is 1.0 L/min with 6% mix ratio. It is merely half the coolant currently used i.e. 2.0 L/min with 10% mix ratio. Minimal use of coolant not only economically desirable for reducing manufacturing cost but also it imparts fewer hazards to human health. Also, sparing use of coolant will eventually transform the turning process into a more environment-conscious manufacturing process.

• 한국터널지하공간학회 논문집
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• 제17권2호
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• pp.107-115
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• 2015

전력구 방식의 지중 송전선로 시공시 TBM 공법이 널리 적용되고 있으며, 세그먼트의 구조적 특징에 따라 강섬유 보강 콘크리트를 이용한 세그먼트의 개발과 사용이 크게 증가하는 추세이다. 그러나 최근 지중 송전선로 구간에서의 전자파 유해성에 대한 문제가 대두되고 있으며, 세그먼트 제작시 투입되는 강섬유의 영향으로 콘크리트의 전기전도성 등이 변화되고, 이로 인하여 더 많은 전자파가 지상에서 계측될 수 있다는 우려가 야기되고 있다. 따라서 본 연구에서는 강섬유 보강 유무 및 사용량 변화조건에 따른 전자파의 차폐효과를 분석하기 위하여, 3가지 조건에 대한 실험체를 제작하여 관련 실험을 수행하였으며, 실험결과 강섬유 보강콘크리트와 전자파 발생 상관관계는 없는 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권6호
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• pp.865-871
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• 2006

Signs of dental discolouration, difficulty in mastication, bony exostosis and debility were observed in cattle from Qingtongxia Ningxia, China where fluoride concentration in drinking water, soil, fodder, serum, bone, teeth, haircoat and urine were significantly higher than the corresponding health site. The problem of fluorosis in beef cattle is attributable to water containing toxic levels of fluoride. The objective of this paper was therefore to evaluate the influence of fluoride on erythrocyte parameters in cattle under high fluoride and low selenium conditions, as well as the protective efficacy of selenium exposure in feedstuff for bovine endemic fluorosis. Sixteen 6 to 7 year-old high fluoride beef cattle were randomly allotted into two groups each with eight cows: high fluoride control group, and supplemented with 0.25 mg/kg selenium per day for 83 days respectively. In addition, eight 6 to 7 year-old normal control beef cattle were selected from a non-high fluoride site. Blood samples were collected on day 0, 30 and 83 for erythrocyte parameters analysis and scanning electronic microscopy. The results indicated that erythrocytes, hemoglobin, packed cell volume values and $Na^+-K^+$ ATPase activity from affected cattle on the high fluoride site were significantly reduced during the period as compared with the corresponding samples of normal control cattle, a great number of echinocytes were present in peripheral blood, and subsequent anaemia. However, affected cattle exposed to selenium revealed increasable erythrocyte parameters, the extent of elevation in these values being dependent on the duration of supplementation with selenium. These findings suggest that fluoride exposure can cause erythrocyte damage, whereas selenium supplementation can antagonize fluoride-induced generation of free radicals and cumulative effects of lipid peroxidation in erythrocytes. Selenium supplementation may help to alleviate the possible hazards associated with bovine endemic fluorosis.

• 한국분말재료학회지
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• 제22권4호
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• pp.234-239
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• 2015

Electronic products are a major part of evolving industry and human life style; however most of them are known to emit electromagnetic waves that have severe health hazards. Therefore, different materials and fabrication techniques are understudy to control or limit transfer of such waves to human body. In this study, nanocomposite powder is dispersed into epoxy resin and shielding effects such as absorption, reflection, penetration and multiple reflections are investigated. In addition, nano size powder (Ni, $Fe_2O_3$, Fe-85Ni, C-Ni) is fabricated by pulsed wire evaporation method and dispersed manually into epoxy. Characterization techniques such as X-ray diffraction, Scanning electron microscopy and Transmission electron microscopy are used to investigate the phase analysis, size and shape as well as dispersion trend of a nano powder on epoxy matrix. Shielding effect is measured by standard test method to investigate the electromagnetic shielding effectiveness of planar materials, ASTM D4935. At lower frequency, sample consisting nano-powder of Fe-85%Wt Ni shows better electromagnetic shielding effect compared to only epoxy, only Ni, $Fe_2O_3$ and C-Ni samples.

• 한국포장학회지
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• 제5권2호
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• pp.42-58
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• 1999

Packaging materials and articles that are used in food contact applications can transfer constituents in the foodstuffs. This kind of risk of possible health hazards to consumers has been generally recognized for a long time with the consequence of establishing corresponding food regulations in most developed countries. However, the language of these laws, their interpretation, and their level of enforcement vary from country to country. Accordingly, the actual migrating levels from packaging materials can be varied depending on the migration testing methods as prescribed in the national legislation in each countries. Therefore, there are needs of elimination of non-tariff trade barriers raised by sanitary and phytosanitary or technical measures under the Final Act of the UR Agreement. In this connection, the EU and USA are currently in an ongoing process of legislation harmonization to overcome potential barriers to free trade. In general, regulations governing component transfer in the USA are more complicated and comprehensive than similar regulations in Europe. In future, standard migration testing procedures for microwave heat susceptor materials and for the use of fatty food simulant should be established and also harmonized among countries. The objective of this investigation is to compare the current regulations for migration testing for plastic containers and packaging materials in USA, EU and Korea or Japan. For those regulations, Korean standards are required to be kept up with the international standards. By doing this, the related Korean regulation could be amended along with the worldwide progress for harmonization.

• Journal of Preventive Medicine and Public Health
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• 제53권5호
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• pp.342-352
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• 2020

Objectives: The aim of this retrospective cohort study was to investigate whether non-alcoholic fatty liver disease (NAFLD) was associated with incident bone mineral density (BMD) decrease. Methods: This study included 4536 subjects with normal BMD at baseline. NAFLD was defined as the presence of fatty liver on abdominal ultrasonography without significant alcohol consumption or other causes. Decreased BMD was defined as a diagnosis of osteopenia, osteoporosis, or BMD below the expected range for the patient's age based on dual-energy X-ray absorptiometry. Cox proportional hazards models were used to estimate the hazard ratio of incident BMD decrease in subjects with or without NAFLD. Subgroup analyses were conducted according to the relevant factors. Results: Across 13 354 person-years of total follow-up, decreased BMD was observed in 606 subjects, corresponding to an incidence of 45.4 cases per 1000 person-years (median follow-up duration, 2.1 years). In the model adjusted for age and sex, the hazard ratio was 0.65 (95% confidence interval, 0.51 to 0.82), and statistical significance disappeared after adjustment for body mass index (BMI) and cardiometabolic factors. In the subgroup analyses, NAFLD was associated with a lower risk of incident BMD decrease in females even after adjustment for confounders. The direction of the effect of NAFLD on the risk of BMD decrease changed depending on BMI category and body fat percentage, although the impact was statistically insignificant. Conclusions: NAFLD had a significant protective effect on BMD in females. However, the effects may vary depending on BMI category or body fat percentage.

• 한국동물위생학회지
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• 제28권3호
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• pp.225-234
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• 2005

Egg has been considered as one of the most important food sources in both nutritionally and economically. However, because the hazards of egg flow chains' complexity from producers to consumers and the insufficiency of cold chain systems in Korea are still remained, preventive measures for egg safety should be established. This experiment was carried out to investigate the changes of egg qualify during storage in order to find out the proper storage condition and eggshell treatment. Eggs were stored at cold condition $(5^{\circ}C,\;relative\;humidity;\;RH:\;65\pm3\%)$ and room Condition $(1st: Sep\; 6\~Nov\; 15,\;2001,\; 10\~30^{\circ}C,\;RH\;41\~86\%,\;2nd;\; Dec\;13,\;2001\~Feb\;21,\;2002,\;4\~23^{\circ}C,\;RH\;36\~89\%)$ after eggshell treatment (unwashed, washed and coated egg) during'10 weeks and examined weight loss and interior quality periodically. Weight loss was in decreasing order room washed > room unwashed > cold washed > cold unwashed > room coated > cold coated egg. And, Haugh unit was decreasing order room washed > room unwashed > room coated > cold washed > cold unwashed > cold coated egg. Our findings show that both coated eggs and refrigeration reduces the rate of decline in Haugh units and egg weight. And, washed eggs had lower average Haugh units and a higher weight loss than any of the eggshell treatment in both storage conditions. Conclusively, for washing eggshell, 150 ppm of sodium hypochlorite, regular exchange and temperature control of water should be consistent and also eggs should be dried immediately after washing. Also, national guidelines for temperature and moisture of storage places, transportation and expiration data should be established to keep quality and weight loss of eggs. Storage temperature that is a critical factor in the quality and safety of eggs should be kept under 12'c and labelling of expiration date should be obligatory.

• 한국산업보건학회지
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• 제28권2호
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• pp.200-210
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• 2018

Objectives: The purpose of this study is to assess the exposure to ozone and total volatile organic compounds(TVOCs) generated during welding work at a shipyard and recommend respiratory protective equipment(RPE) adequate against these hazards. Method: Ozone was collected for about 30 minutes at two-minutes intervals using a direct reading instrument, specifically an ozone analyzer(Serinus 10, Ecotech, Australia). TVOCs were collected for about 30 minutes at three-minute intervals using a portable GC (Alpha 115, Synspec BV, the Netherlands), and were determined simultaneously by area sampling at the welding plume closest to the welder's breathing zone. The total measurements were 162 for ozone($CO_2$ welding 47, TIG 60, stick 55), and 136 for TVOCs($CO_2$ 65, TIG 50, stick 21). Based on these measurements, a literature survey was conducted to assess the adequacy of RPE. Results: Relative to Korean OEL, measurements above STEL 0.2 ppm were 23.4% for $CO_2$, 63.3% for TIG and 14.5% for stick welding. There were significant differences(p=<0.0001) among welding types. Compared with ACGIH peak exposure of 0.4 ppm for ozone, which is not applied in Korea, $CO_2$ welding exceeded it by 10.6%, TIG by 40.0% and stick by 7.3%. Although it was not feasible to compare them directly since there are no Korean OEL, TVOCs had very high levels similar to the concentrations before moving into a new apartment and about 10-20 times the indoor air quality recommendations for some individual measurements. Conclusions: As ozone removal RPE has been recommended in welding environments for a long time(Lunau, 1967), this fact was demonstrated based on the results of the on-site work environment measurements(ozone and TVOCs). In conclusion, for all welding at a shipyard, gas/vapor and particulate combination RPE are recommended. If this is not possible, it should at least be present for TIG welding.