• Asian-Australasian Journal of Animal Sciences
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• 제12권6호
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• pp.846-849
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• 1999

Angus postweaning daily gain (PWDG) were analyzed to investigate heterogeneous variance by sex. A set of data (16,239 records) was divided into six sub-data sets according to level of environment. REML estimation was conducted by a multitrait model, where PWDG in each sex was treated as a separate trait. Estimates showed diversity among environmental levels, where the heritability for heifers was high in good environment but low in poor environment. The bull's estimates varied among environmental levels. The largest heterogeneity of phenotypic variance between sexes was estimated in a data set of the poor environment level. The genetic correlations between the heifer's PWDG and the bull's PWDG were high in the good environment and low in the poor environment (-0.17). The results suggest existence of genotype by sex interaction in the poor environment.

• Asian-Australasian Journal of Animal Sciences
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• 제12권2호
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• pp.253-260
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• 1999

Occurrences of foodborne disease outbreaks are increasing in Korea. Among the outbreaks, Salmonella, Staphylococcus aureus, Vibrio parahaemolyticus are the most important organisms and meat and meat product the major sources of infection. Hazard Analysis Critical Control Point system is a process control system designed to identify and prevent microbial and other hazards in food production. It is considered to be the best process management system by the National Advisory Committee for Microbiological Criteria for Foods, the National Academy of Science, and the Codex Alimentarius Commission. The Korean Ministry of Health and Welfare established a legal basis for the implementation of the HACCP system in Article 32-2 (Hazard Analysis Critical Control Point) of the Food Sanitation Act in December 1995. The Ministry of Agriculture and Forestry has granted research funds for the development of this model system for application to traditional food products and processed fishery products as well as to raw meats. Implementation of the HACCP system is an important step and the Korean food industry and the Government are focused on ensuring food safety in Korea.

• Journal of Nutrition and Health
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• 제36권9호
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• pp.966-973
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• 2003

The purpose of this study was to investigate the utilization level of forecasting methods in contract foodservice management companies. Questionnaires were distributed and collected from 30 foodservice management companies contracted with universities and 49 university foodservices in Seoul and Kyungki area. Statistical data analysis was performed using SPSS/WIN 10.0 based on the production records of Yonsei University foodservices and the weather reports from a meteorological observatory. The results of this study were as follows: 1) The objectives of the fore-casting systems were identified as saving costs through eliminating the leftover, meeting the customer demands, and improving efficiency in food preparation.2) All of the university foodservices were already performing the forecasting methods but in foodservice management companies as a whole,89.7 percents were applying the method and only 55.2 percents had the separate forecasting department. 3) A large number of foodservice staffs in the head office (65.5％) answered that they often utilized intuitive estimates based on the past experiences and records for forecasting while 65.3% managing staffs in the university foodservices answered the same.4) Both in the head office and university foodservices, actual number of meals served were recorded. In the head office, mostly estimated numbers and actual numbers of meals were recorded while estimated, prepared, and actual numbers of meals served were recorded for most of the cases in university foodservices. 5) The primary factors considered for forecasting were the actual production records for the last month, the customer preference for the selected menu items, and the specific day of the week.

• International journal of advanced smart convergence
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• 제12권3호
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• pp.104-108
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• 2023

This paper deals with research on innovative systems using Python-based artificial intelligence technology in the field of plant growth monitoring. The importance of monitoring and analyzing the health status and growth environment of plants in real time contributes to improving the efficiency and quality of crop production. This paper proposes a method of processing and analyzing plant image data using computer vision and deep learning technologies. The system was implemented using Python language and the main deep learning framework, TensorFlow, PyTorch. A camera system that monitors plants in real time acquires image data and provides it as input to a deep neural network model. This model was used to determine the growth state of plants, the presence of pests, and nutritional status. The proposed system provides users with information on plant state changes in real time by providing monitoring results in the form of visual or notification. In addition, it is also used to predict future growth conditions or anomalies by building data analysis and prediction models based on the collected data. This paper is about the design and implementation of Python-based plant growth monitoring systems, data processing and analysis methods, and is expected to contribute to important research areas for improving plant production efficiency and reducing resource consumption.

• 한국식품위생안전성학회지
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• 제18권4호
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• pp.202-210
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• 2003

두부는 양질의 단백질을 주성분으로 한 대중 식품으로 소비량이 계속 증가하고 있다. 두부는 변질 발생 가능성이 높은 고위험 식품에 해당되지만, 두부제조업체는 82.4%가 종업원 5인 이하 소규모로 체계적인 식품안전관리를 기대하기 어려운 실정이다. HACC(Hazard Analysis Critical Control Point : 위해요소 중점관리제도)는 식품안전성과 비용절감을 ㅎ율적으로 확보할 수 있는 체계적, 지속적인 공정관리 방법이다. 본 연구는 두부제조업체의 실정에 맞는 HACCP시스템을 개발하여 적용하고 그 성과를 확인함으로써 두부 HACCP 시스템의 일반적 모델을 확정하고 다른 중소업체 및 대기업으로 이를 확산시키려고 시행하였다. 규모별 4개 두부제조업체를 대상으로 판 두부와 포장 판 두부의 HACCP 계획을 Codex의 HACCP 적용 지침에 따라 현장 실부자와 협력하여 수립하였다. HACCP 팀 편성, HACCP 범위 및 목적의 설정, 제품 기술 및 사용자 의도의 결정, 공정도 작성 및 현장 검증, 위해 분석 및 예방책 식별, CCP(중요관리점)의 식별, CL(관리할계기준) 설정, CCP 모니터링 시스템 수립, 개선조치 설정, 검증절차 수립, 기록 및 문서화의 순서로 HACCP 계획을 작성하였다. 공정 중 CCP는 선별 공정, 냉각 공정, 열처리 공정(판 두부는 제외)으로 결정하였고, 각각의 HACCP 계획에 따라 관리하였다. 제정된 HACCP시스템을 3개월간 시행한 후 그 성과를 HACCP 적용 이전과 이후의 미생물 검사를 통하여 확인하였다. 검사결과 HACCP 적용 이전의 판두부 플라스틱 상자, 포장재, 완제품의 일반 세균수가 HACCP 적용 이후에 크게 감소되었다.(P<0.1) 두부제조업체의 실정에 맞는 HACCP 시스템을 적용함으로써 위생관리 및 식품안정성 확보에 큰 성과가 있음이 확인되었다.

• International Journal of Stem Cells
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• 제9권2호
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• pp.186-191
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• 2016

Background and Objectives: With the global demand for dairy protein for consumption growing annually, there has been increasing activity in the research field of dairy protein synthesis and production. From a manipulation perspective, it is more difficult to use live cattle for laboratory studies on the production of milk as well as of dairy protein such as casein, as compared with using laboratory animals like rodents. Therefore, we aimed to develop a mouse model of bovine mammary alveolar ducts for laboratory-scale studies. We studied the formation of the bovine mammary gland ductal structure by transplanting the MAC-T bovine alveolar cell line into mice. Methods and Results: MAC-T cells ($1{\times}10^7$) were suspended in Matrigel and injected into the dorsal tissue of 8-week-old male BALB/C nude mice. Histological analysis of tissue dissected from the MAC-T cell-transplanted mice after 6 weeks showed the typical morphology of the tubuloalveolar female gland, as well as glands made up of branching ducts that were surrounded by smooth muscle with small alveoli budding off the ducts. In addition, the epithelial markers CK14 and CK18 were expressed within the duct-like structure. Prolactin was detected in the duct interior in these CK14+ and CK18+ cells but not in the non-transplanted MAC-T cells. Conclusions: These results showed that duct-like tissue had been successfully formed after 6 weeks of transplantation of the CK14+ and CK18+ MAC-T cells into mice dorsal tissue. This mouse model will be a useful tool for further research on the bovine mammary gland.

• Asian-Australasian Journal of Animal Sciences
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• 제32권11호
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• pp.1789-1800
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• 2019

Objective: Although alveolar macrophages play a key role in the respiratory immunity of livestock, studies on the mechanism of differentiation and survival of alveolar macrophages are lacking. Therefore, we undertook to investigate changes in the lipid metabolism and survival rate, using 3D4/31 macrophages and Dudleya brittonii which has been used as a traditional asthma treatment. Methods: 3D4/31 macrophages were used as the in vitro porcine alveolar macrophages model. The cells were activated by exposure to phorbol 12-myristate 13-acetate (PMA). Dudleya brittonii extraction was performed with distilled water. For evaluating the cell survival rate, we performed the water-soluble tetrazolium salt cell viability assay and growth curve analysis. To confirm cell death, cell cycle and intracellular reactive oxygen species (ROS) levels were measured using flow cytometric analysis by applying fluorescence dye dichlorofluorescein diacetate and propidium iodide. Furthermore, we also evaluated cellular lipid accumulation with oil red O staining, and fatty acid synthesis related genes expression levels using quantitative polymerase chain reaction (qPCR) with SYBR green dye. Glycolysis, fatty acid oxidation, and tricarboxylic acid (TCA) cycle related gene expression levels were measured using qPCR after exposure to Dudleya brittonii extract (DB) for 12 h. Results: The ROS production and cell death were induced by PMA treatment, and exposure to DB reduced the PMA induced downregulation of cell survival. The PMA and DB treatments upregulated the lipid accumulation, with corresponding increase in the acetyl-CoA carboxylase alpha, fatty acid synthase mRNA expressions. DB-PMA co-treatment reduced the glycolysis genes expression, but increased the expressions of fatty acid oxidation and TCA cycle genes. Conclusion: This study provides new insights and directions for further research relating to the immunity of porcine respiratory system, by employing a model based on alveolar macrophages and natural materials.

• 성인간호학회지
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• 제22권6호
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• pp.582-593
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• 2010

Purpose: Health care professionals represent an immediately available resource to promote smoking cessation. However, the content in smoking cessation intervention courses in most institutions is insufficiently covered due to the limited number of class hours. The purpose of this study was to develop a comprehensive tobacco cessation-web-based, multimedia, educational program for a range of medical professionals. Methods: Based on Jung's Teaching and Learning Structure Plan Model, a development process was proposed comprising four stages: (1) analysis, (2) planning, (3) production, and (4) operation/evaluation. The effectiveness of the program was tested using quasi-experimental design, and the participants in experimental group were required to complete the program. Changes in the level of knowledge and attitude were measured. Results: The educational program developed includes nine topics and 26 sub-topics. A total of 180 storyboards, 15 videos, and 27 pictures were made. After the education, the level of knowledge was significantly increased in the experimental group. Conclusion: This web-based program can be recommended as a potential medium for health care professionals to use in counseling smoking cessation. The study findings also indicated that the program may be either offered as a teaching aid or utilized concurrently with lectures for students studying health care-related topics.

• 대한간호학회지
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• 제34권6호
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• pp.994-1003
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• 2004

Purpose: This study was to develop Web-based multimedia content for Physical Examination and Health Assesment. Method: The multimedia content was developed based on Jung's teaching and learning structure plan model, using the following 5 processes: 1) Analysis Stage, 2) Planning Stage, 3) Storyboard Framing and Production Stage, 4) Program Operation Stage, and 5) Final Evaluation Stage. Results: The web based multimedia content consisted of an intro movie, main page and sub pages. On the main page, there were 6 menu bars that consisted of Announcement center, Information of professors, Lecture guide, Cyber lecture, Q&A, and Data centers, and a site map which introduced 15 week lectures. In the operation of web based multimedia content, HTML, JavaScript, Flash, and multimedia technology(Audio and Video) were utilized and the content consisted of text content, interactive content, animation, and audio & video. Consultation with the experts in context, computer engineering, and educational technology was utilized in the development of these processes. Conclusions: Web-based multimedia content is expected to offer individualized and tailored learning opportunities to maximize and facilitate the effectiveness of the teaching and learning process. Therefore, multimedia content should be utilized concurrently with the lecture in the Physical Examination and Health Assesment classes as a vital teaching aid to make up for the weakness of the face-to- face teaching-learning method.

• 식품보건융합연구
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• 제10권2호
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• pp.7-11
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• 2024

Glutathione (GSH) is a vital compound composed of glutamic acid, cysteine, and glycine, crucial for cellular functions including oxidative stress defense and detoxification. It has widespread applications in pharmaceuticals, cosmetics, and food industries due to its antioxidant properties and immune system support. Two primary methods for GSH synthesis are enzymatic and microbial fermentation. Enzymatic synthesis is efficient but costly, while microbial fermentation, particularly using yeast strains like Candida albicans, offers a cost-effective alternative. This study focuses on genetically modifying C. albicans mutants, specifically targeting glutathione reductase (GLR1) and gamma-glutamylcysteine synthetase (GCS1) genes, integral to GSH synthesis. By optimizing these mutants, the research aims to develop a model for efficient GSH production, potentially expanding its applications in the food industry.