• YAKHAK HOEJI
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• v.40 no.4
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• pp.411-417
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• 1996

Epidermal growth factor(EGF), a potential healing agent for wounds and burns, has been formulated to topical gels with the hydrophilic polymers, Carbopol 934P. Physicochemical c haracteristics of the aqueous gels were evaluated by rheological properties and pH changes on storage. The gels were relatively stable at $4^{\circ}C$ and room temperature showing no changes in pH for two weeks, and revealed the rheogram of shear thinning plastic flow with the yield values in the range of 40 to 70dyne/$cm^2$. In vivo healing effects of different gel formulations have been compared with water-soluble and oleaginous ointments in full-thickness wound mouse model. The gel systems resulted in better wound healing effects than the other ointments. Furthermore, liposomal Carbopol gel has been developed by the addition of EGF-containing liposomal suspension into the Carbopol gel. The enhanced wound healing effects have been observed in the liposomal gel system, compared to the other gels and conventional ointments.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2005.05a
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• pp.29-32
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• 2005

It is recognised that a permeability waterproof agent have been extensively used for concrete, in these days. In present paper shows effective practical scheme of a permeability waterproof agent in construction market that is to develope durability of concrete structure as apply to job site through the examination physical and chemical property of a permeability waterproof agent. It is indentified that can be maintain waterproofing performance, moreover it makes satisfactory result of permeation resistance as applying siliceous waterproof agent.

• Herbal Formula Science
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• v.25 no.2
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• pp.123-134
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• 2017

Wounds are commonly created during almost every kind of surgery, trauma and skin diseases. Delayed wound healing affects a plenty of patients and requires prolonged treatments that seriously reduce the quality of life for patients. Skin damage involving large areas or great severity can lead to disability or even death. Wound healing involves a complicated series of actions, of various tissues and cell lineages, concerning inflammation, migration, proliferation, reepithelialization, and remodeling. Sopung-san is reported to have anti-inflammatory effect and has been used for various skin diseases such as allergic dermatitis and atopic dermatitis. In this study, the hypothesis that oral treatment with Sopung-san could enhances healing potential on rat full thickness skin wounds was tested. Twenty young male Sprague-Dawley rats were used for the studies. A full-thickness skin wound was made on the dorsal skin of the rats. Either Sopung-san water extract (SPS) or saline (Control) was orally administrated every day. The wound area was measured and the percentages of wound contraction, wound healed and wound epithelization were calculated. Wound tissue samples were excised following injection for histopathological and immunohistological examination. Wound area in rats of SPS group significantly was decreased compared to Control. SPS group showed significant promotion of wound healing compared to Cotrol group in the percentages of wound contraction, wound healed and wound epithelization. Histopathological examination revealed that SPS induces neo-vascularization potential in wound healing process. SPS treatment in rats significantly accelerated cutaneous wound healing in the neo-vascularization process by increasing VEGF and $TGF-{\beta}1$ synthesis. The results suggest that Sopung-san affects key cellular processes responsible for wound repair and point to a unique potential for this molecule in the therapy of skin wounds, particularly as an angiogenic agent.

• Journal of Veterinary Science
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• v.22 no.2
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• pp.27.1-27.13
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• 2021

Background: Ozone is an antimicrobial agent that in experimental and case-control studies has been found to exert a positive effect on wound healing. Wild and pet chelonians frequently present insidious wounds exhibiting secondary infections and/or delayed healing. Objectives: Evaluate the effects of topical ozonated sunflower oil on second-intention healing of acute experimental skin wounds in red-eared sliders (Trachemys scripta elegans). Methods: Randomised within-subject controlled study; Group 1 (n = 24) was used to assess clinical healing features; Group 2 (n = 12) was used for histological evaluation in which two sets of wounds were biopsied at 2, 7, 14, 21, 28 and 42 days over the course of the cicatrisation process. A single 6 mm diameter wound was made on each rear limb and topical ozonated (950 peroxide value) and non-ozonated sunflower oil were applied daily for one week on treated and contralateral control wounds, respectively. Results: Mean wound size was significantly lower in the ozone-treated group at day 28 (p < 0.0001) with differences of clinical relevance (74.04% vs. 93.05% reduction of initial wound size). Histologically, the acute inflammatory reaction was enhanced in treated wounds, with significantly higher numbers of heterophils (p = 0.0016), lymphocytes (p < 0.001) and fibroblasts (p < 0.001). Conclusions: Daily topical application of ozonated sunflower oil over the course of one week improved the healing of acute, full-thickness skin wounds in chelonians. This clinical outcome was histologically correlated with an enhanced acute inflammatory reaction, as well as the production and remodelling of collagen fibres.

• Archives of Craniofacial Surgery
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• v.20 no.5
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• pp.289-296
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• 2019

Background: Topical hemostatic agents are used when ligation, electrocauterization, or other conventional hemostatic methods are impractical. Because a hemostatic agent is a foreign body, it can cause foreign body reactions, inflammation, and infections that can interfere with the wound healing process. Therefore, we should select hemostatic agents after considering their effects on wound healing. Here, we compared the effects of hemostatic agents on wound healing in a rectus abdominis muscle defect in rats. Methods: Twelve Sprague Dawley rats were subjected to creation of a $6{\times}6mm$ defect in the rectus abdominis muscle and divided into four groups: control group; group A, Tachosil fibrin sealant patch; group B, Surgicel Fibrillar oxidized regenerated cellulose; and group C, Surgicel Snow oxidized regenerated cellulose. For the histologic analysis, biopsies were performed on the 3rd, 7th, and 27th days. Results: The foreign body reaction was the weakest in group A and most significant in group C. The inflammatory cell infiltration was the weakest in group A and similar in groups B and C. Muscle regeneration differed among periods. The rats in group A were the most active initially, while those in group C showed prolonged activity. Conclusion: Tachosil and Surgicel administration increased inflammation via foreign body reactions, but the overall wound healing process was not significantly affected. The increased inflammation in the Surgicel groups was due to a low pH. We recommend using Tachosil, because it results in less intense foreign body reactions than Surgicel and faster wound healing due to the fibrin action.

• Journal of the Korean Recycled Construction Resources Institute
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• v.8 no.1
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• pp.134-142
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• 2020

In this study, in order to evaluate the self-healing performance of cement composites the self-healing test method and the analysis method were suggested by applying constant water head permeability test, chloride migration test and repeated bending test. The method of making a cracked specimen and controlling crack width are also proposed. Constant head water permeability test can evaluate the healing performance by using the decreasing rate of water flow passing through the crack zone of a specimen. Furthermore, the equivalent crack width can be used to intuitively investigate the healing effect with healing period. The chloride migration test can evaluate the healing rate by the decreasing rate of the diffusion coefficient obtained by ASTM C 1202. Mechanical healing performance can be evaluated using ISR and IDR estimated from load vs. CMOD relationship graph obtained through the repeated bending test. Finally, the applicability of proposed self-healing evaluation methods was examined by testing mortar specimens with or without self-healing agents.

• Journal of Oral Medicine and Pain
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• v.24 no.2
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• pp.137-143
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• 1999

Aucubin, the natural product, which is isolated from Aucuba japonica, has a variety of pharmacological effects such as liver-protective function, inhibition of liver RNA and protein biosynthesis, hypotensive activity and antimicrobial effect, etc. This study was performed to investigate the effects of iridoid compounds on wound healing. The author prepared 0.1% aucubin solution and 0.1% aucubin ointment as an active form, aucubigenin to which aucubin was converted by ${\beta}$-glucosidase. Artificial surgical wound was made on either 1cm lateral side of the dorsal midline along the axis of spine of Sprague-Dawley rats under sterile technique. Application of 0.1% aucubin solution or 0.1% aucubin ointment to surgical wound was done daily. Light microscopic examination was performed on the postsurgical 3 days, 5 days, and 9 days. The 0.1% aucubin solution group epithelialized earlier than the control group and the fibrosis of granulation tissue of both aucubin groups were more prominent than the control group. Collectively, this study suggests the possibility of aucubin as a topical agent. Further research should be performed on the mechanism of aucubin on wound healing and proper formulation for effective topical agents.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.34 no.3
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• pp.53-59
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• 2018

When manufacturing self-healing concrete using bacteria, nutrients are added to increase the activity of the bacteria. Although many researches have focused on the effects of nutrients containing bacterial healing agent on concrete, few have studied the effects of sole nutrient on self-healing of cement composites. Bacterial nutrients, like commercial chemical admixtures, affect hydration characteristics such as flow, setting, hydration heat, mechanical strength of cement composites and also affect the self healing of cement composites by hydration of unhydrated particles. In this study, effect of the four nutrient commonly used in the existing literature on the hydration characteristics of cement composites by its addition was investigated. Flow, setting time, hydration heat, compressive strength have studied for each nutrients added by 1.5% and 3% of cement weight. Experimental results shows that urea and calcium-nitrate can be used up to 3% without significant detrimental effect on cement composites. Addition of calcium-lactate up to 1.5% show better compressive strength than control, but addition of 3% show almost non-hydration. Yeast extract shows detrimental effects on the composites regardless of the amount added.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2005.04a
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• pp.150-153
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• 2005

• Ocean and Polar Research
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• v.30 no.4
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• pp.467-472
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• 2008

Antarctic krill has a strong proteolytic enzyme system, which comes from a combination of several proteases. This powerful activity can be easily detected by krill's superior post mortem autolysis. Mammalian skin consists of epidermis and dermal connective tissue, and functions as a barrier against threatening environments. A clot in a wound site of the skin should be removed for successful skin regeneration. Epithelial cells secrete proteases to dissolve the clot. In previous studies Antarctic krill proteases were purified and characterized. The proteolytic enzymes from Antarctic krill showed higher activity than mammalian enzymes. It has been suggested that these krill clean up the necrotic skin wound to induce a natural healing ability. The enzymes exhibited additional possibilities for several other biomedical applications, including dental plaque controlling agent and healing agent for corneal alkali burn. Considering that these versatile activities come from a mixture of several enzymes, discovering other proteolytic enzymes could be another feasible way to enhance the activity if they can be used together with krill enzymes. Molecular cloning of the krill proteases should be carried out to study and develop the applications. This review introduces possible roles of the unique Antarctic krill proteases, with basic information and suggestion for the development of an application to skin regeneration.