Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Effect of topical ozonated sunflower oil on second intention wound healing in turtles: a randomised experimental study

  • Ginel, Pedro J. (Department of Animal Medicine & Surgery, Faculty of Veterinary Medicine, University of Cordoba) ;
  • Negrini, Joao (Department of Anatomy & Comparative Pathology, Faculty of Veterinary Medicine, University of Cordoba) ;
  • Guerra, Rafael (Zoological Garden of Cordoba) ;
  • Lucena, Rosario (Department of Animal Medicine & Surgery, Faculty of Veterinary Medicine, University of Cordoba) ;
  • Ruiz-Campillo, Maria T. (Department of Anatomy & Comparative Pathology, Faculty of Veterinary Medicine, University of Cordoba) ;
  • Mozos, Elena (Department of Anatomy & Comparative Pathology, Faculty of Veterinary Medicine, University of Cordoba)
  • Received : 2020.10.14
  • Accepted : 2021.02.04
  • Published : 2021.03.31
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Abstract

Background: Ozone is an antimicrobial agent that in experimental and case-control studies has been found to exert a positive effect on wound healing. Wild and pet chelonians frequently present insidious wounds exhibiting secondary infections and/or delayed healing. Objectives: Evaluate the effects of topical ozonated sunflower oil on second-intention healing of acute experimental skin wounds in red-eared sliders (Trachemys scripta elegans). Methods: Randomised within-subject controlled study; Group 1 (n = 24) was used to assess clinical healing features; Group 2 (n = 12) was used for histological evaluation in which two sets of wounds were biopsied at 2, 7, 14, 21, 28 and 42 days over the course of the cicatrisation process. A single 6 mm diameter wound was made on each rear limb and topical ozonated (950 peroxide value) and non-ozonated sunflower oil were applied daily for one week on treated and contralateral control wounds, respectively. Results: Mean wound size was significantly lower in the ozone-treated group at day 28 (p < 0.0001) with differences of clinical relevance (74.04% vs. 93.05% reduction of initial wound size). Histologically, the acute inflammatory reaction was enhanced in treated wounds, with significantly higher numbers of heterophils (p = 0.0016), lymphocytes (p < 0.001) and fibroblasts (p < 0.001). Conclusions: Daily topical application of ozonated sunflower oil over the course of one week improved the healing of acute, full-thickness skin wounds in chelonians. This clinical outcome was histologically correlated with an enhanced acute inflammatory reaction, as well as the production and remodelling of collagen fibres.

Keywords

Acknowledgement

The authors thank Miguel Angel Hormigo (DVM), from Ozonovet S.L (Cadiz, Spain) for providing ozonated sunflower oil samples and for his expert advice on the manuscript.

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