• The Korean Journal of Zoology
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• v.8 no.1
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• pp.8-10
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• 1965

1. We have observed the frequency , geographi variation and microgeographic variation of the elytral pattern types and the elytral edge of the population of Lady-beetle, Harmonia axyridis PALLACE, collected in Jinju and other 6 localitites in South Korea. 2. the number of specimen collected were 6,625 in which Succinea was 90.32% : Axyridis ; 0.74% : Spectabilis , 3.82% ; and Conspicua 5.07%. 3. We have found that 92.26% of all specimens have elytral edge. 4. There were some differences in the compositional of the elytral pattern types and elytral edge to the population among the Western seaside , inland and southern seaside of South Korea , whereas as great difference was in the compositional proportion tot he population of Spectabilis between the western and southern seaside . There was also a gret difference of the elytral edge between the inland and southern seaside. 5. A micro-geographic variation was observed in Jinju , located on southern area of South Korea. In the samples collected from the pine trees at Jinju area the Succinea occupied 73.25%, and 69.61 % were found to be provided with elytral edge, while in the general population 92.46% of specimen were Succinea and 98.35% were found to have elytral edge.

• Journal of Bio-Environment Control
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• v.13 no.1
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• pp.56-60
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• 2004

The aphidophagous lady beetle, Harmonia axyridis, is induced to undergo reproductive diapause by low temperature treatment. The diapause of the adult beetle can be maintained without supplying the diet up to 5 months at $4^{\circ}C$. The diapause can be induced any time including during the summer, allowing the beetle to be supplied to the field for the control of aphids through the year. Fecundity and longevity after the reproductive diapause is though to be satisfactory enough for the control of aphids in the field, Methoprene, an analog of reproductive hormone of JH, increases the early fecundity of the beetle.

• Korean journal of applied entomology
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• v.48 no.4
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• pp.459-466
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• 2009

There was different between two differential geographical and environmental condition areas on elytra color expression patterns of the multicolored Asian lady beetles (Harmonia axyridis). Especially, it was investigated that expression rates of melanic patterns (conspicua, spectabilis and axyridis) relatively increased in overwintering populations collected in highly mean temperature and longer cumulative daylength area. In addition, in the same collection site, the seasonal difference had influenced on color patterns of H. axyridis. Although these effects didn't were not observed in the laboratory, environmental conditions such as temperature or cumulative daylength might be factors that gave an effect on color pattern formation.

• Journal of Microbiology and Biotechnology
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• v.22 no.11
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• pp.1588-1590
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• 2012

We compared the mRNA expression profile of the Harmonia axyridis larvae that were either untreated or treated with LPS. The extracted mRNAs were subjected to ACP RT-PCR analysis using a combination of arbitrary primers and oligo (dT) primer. Among the 47 DEGs differentially expressed, we identified a cDNA showing homology with defensin-like antibacterial peptide. The cDNA showed a putative 32-residue signal sequence and a 50-residue mature peptide named harmoniasin. We also investigated the antibacterial activity of the harmoniasin analog, which exhibited potent antibacterial activities against Gramnegative and -positive bacteria strains and it also evidenced no hemolytic activity.

• Animal cells and systems
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• v.16 no.6
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• pp.479-487
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• 2012

Biological attachment device is optimized in insect legs for attachment onto the variety of natural substrate. We have studied the microstructural characteristics of the tarsal appendages in the ladybug Harmonia axyridis using scanning electron microscopy to reveal the attachment system of their legs. The attachment devices are composed of claws and adhesive pads. The claws are connected with pretarsal segment, and their apical diverged hooks are developed to hold rough substrates. In contrast, the adhesive pads have an adhesive function onto smooth surface. The pads are interspersed at the ventral part of each tarsomere, and are composed of two kinds of hairy setae. The discoid tip seta (DtS) has a spoon-shaped endplate usually with a rounded concave structure, whereas the pointed tip seta (PtS) has a pointed tip, usually with a hooked endplate. While the PtS is broadly localized concentrically on the marginal area of both the proximal and distal pads, the DtS can be seen at the central areas of each adhesive pad except for the hind legs. Our findings demonstrate the presence of the direction-dependence pattern of the fibrillar system as well as a functional modification of the tenent setae to achieve proper contact with almost any kind of substrates.

• Journal of Life Science
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• v.23 no.4
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• pp.495-500
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• 2013

Harmonia axyridis is known to display diverse biological activities, such as growth promotion. However, few studies have investigated the effect of H. axyridis on inflammation of the skin. In this study, we explored the anti-inflammatory effect of the Harmoniasin gene fragment (HaGF) peptide from H. axyridis on macrophage cells. During the entire experimental period, 5, 25, 50, and 100 ${\mu}g/ml$ of HaGF showed no cytotoxicity. However, at these concentrations, HaGF inhibited the activity of iNOS and COX-2 by 51% and 49%, respectively. In addition, the HaGF extract reduced the release of inflammatory cytokines, including TNF-a and IL-6. Therefore, HaGF has been LPS-induced macrophage Raw 264.7 cells could be expected from the inhibitory effects of the inflammatory.

• Korean journal of applied entomology
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• v.50 no.2
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• pp.157-165
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• 2011

Enterobacteria were isolated in the gut of the predacious multicolored Asian ladybird beetle, Harmonia axyridis, and their effects to the development of H. axyridis were examined. Populations of H. axyridis in this experiment were collected from Kimjae at Cheonbuk province (JK population), Geumsan at Chungnam province (CK population) and laboratory population at Laboratory of Insect Physiology in Chungnam National University, Daejeon. Thirty-four enterobacteria isolates were purified and isolated from the digestive tract of H. axyridis, and a total of 4 strains were classified into group by analysis of 16S rRNA gene sequences. About 70% of total isolates were phylogenetic groups of Bacillus genus and Staphylococcus genus, and they were commonly separated from the digestive tract of H. axyridis. After investigating their susceptibility against antibiotics with 18 representative enterobacteria isolates, ofloxacin and penicillin were selected for examination in this study of their ability to inhibit the growth of all of isolates. In order to remove the enterobacteria from the aphids, ofloxacin and penicillin were given to the green peach aphid, Myzus persicae, and the turnip aphid, Lipaphis erysimi. These aphids were provided to H. axyridis as prey. The weight of pupa, developmental periods of each larval instar, the number of eggs and their hatching ratio of H. axyridis with treatment aphids were lower compared with non-treatment aphids. Staphylococcus saprophyticus is a representative enterobacteria and commonly isolated from the digestive tract of H. axyridis. In the absence of S. saprophyticus, the developmental periods of each larval instar increased; however, the weights of pupa, the number of eggs, and their hatching ratio decreased.

• Food Science and Preservation
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• v.13 no.4
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• pp.513-518
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• 2006

Insect resources have been widely recognized that seven millions of insects exhibit it own biological activity by whole body or its metabolic intermediates. In order to investigate antioxidant activity and compare the cyclooxygenase-2 promoter activity from insect extract, we tested in vitro antioxidant assays and cyclooxygenase-2 promoter assay in Coccinella septempunctata Linne and Harmonia axyridis extracts have the anti-oxidant and cyclooxygenase-2 inhibition activities, we examined the anti-oxidant assays including DPPH, FRAP and linoleic acid, ana inhibition of cyclooxygenase-2 expression using a cyclooxygenase-2 promoter-inserted stable cell line. We found that Harmonia axyridis Pallas extract had potentials to anti-oxidant activity and inhibited about 25% of cyclooxygenase-2 transcription activity. These findings indicate that Coccinella septempunctata Linne and Harmonia axyridis Pallas extracts could be an useful insect resource for agrobiotechnological purposes.

• Korean Journal of Agricultural Science
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• v.28 no.1
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• pp.18-26
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• 2001

Application of the Asian ladybird (Harmonia axyridis) to control several species of aphids in the plastic green houses in mind, control effects of aphid populations regulated by the Asian ladybird were observed. The green peach aphid, the turnip aphid, and the cotton aphid were present on mustard plants, Angelia utlis, ornamental kales, and egg plants at greenhouses in spring, summer, and winter. Adults and larvae of the Asian ladybird used in experiments were collected from aggregated sites at Taejon in the autumn and reared on the cotton aphid in the laboratory. In winter, more number of adults and larvae of ladybirds than in other seasons were needed to control aphid population in successively double plastic greenhouses with supplied subterranean water for keeping warmth. In spring and summer, it was possible to keep the aphid populations low when necessary by manipulating ladybird populations according to the density of aphids. On the other hand, the innate increasing rate of aphid, the aphid population density at the time of applying ladybird, the predacious ability of ladybird at specific developmental stages, and needed periods should be taken into account to control aphids. In addition, the environmental factors, for example, optimum temperature and humidity should be considered to be biologically effective when ladybirds are released to greenhouses.

• Korean journal of applied entomology
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• v.45 no.1 s.142
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• pp.31-36
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• 2006

Inflammation in the brain has known to be associated with the development of a various neurologiacal diseases. The hallmark of neuro-inflammation is the activation of microglia, brain macrophage. Pro-inflammatory compounds including nitric oxide(NO) are the main cause of neuro-degenerative disease such as Alzheimer's disease. In the study, we examined whether Harmonia axyridis extracts inhibit the NO production by a direct method using Griess reagent, western blotting and by RT-PCR(Reverse Transcription-Polymerase Chain Reactionin) the gene expression of inducible nitric oxide synthase(iNOS). Distilled water$(H_2O)$ and methanol(MeOH) extracts of H. axyridis inhibited the protein expression of TNF-a(Tumor Necrosis Factor) and IL-6(Interleukin) in LPS (Lipopolysaccharide) stimulated BV-2 cells at the concentration of 100 ng/ml. Incubation of BV-2 cells with the extracts of $H_2O$ of MeOH inhibited the LPS induced NO and iNOS protein. And this inhibition of iNOS protein is concordant with the inhibition of iNOS mRNA expression. These data suggested that H. axyridis extracts may play a crucial role in inhibiting the NO production.