• 제목/요약/키워드: Hand controls

검색결과 246건 처리시간 0.023초

Utilization of the bar gene to develop an efficient method for detection of the pollen-mediated gene flow in Chinese cabbage (Brassica rapa spp. pekinensis)

  • Lim, Chaewan;Kim, Sunggil;Choi, Yeonok;Park, Young-doo;Kim, Sung Uk;Sung, Soon-Kee
    • Plant Biotechnology Reports
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    • 제1권1호
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    • pp.19-25
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    • 2007
  • To develop an efficient screening method for detection of the transgene in Chinese cabbage (Brassica rapa spp. pekinensis) utilizing Basta spray, optimal conditions for Basta application were examined in this study. Two transgenic Chinese cabbage lines were obtained through Agrobacterium-mediated transformation and used as transgenic positive controls in the Basta screening experiment. Differential concentrations of glufosinate-ammonium were sprayed into three different growth stages of 12 commercial Chinese cabbage cultivars. The results showed that no plants could survive higher than 0.05% glufosinate-ammonium, and plants at the 2-3 leaf stage were most vulnerable to glufosinate-ammonium. On the other hand, no damage was observed in the transgenic control plants. Reliability of the Basta spray method was proven by showing perfect co-segregation of the tolerance to glufosinate-ammonium and the presence of the bar gene in T1 segregating populations of the transgenic lines, as revealed by both PCR and Southern blot analyses. Using the developed Basta screening method, we tried to investigate the transgene flow through pollen dispersal, but failed to detect any transgene-containing non-transgenic Chinese cabbages whose parents had been planted adjacent to transgenic Chinese cabbages in field conditions. However, the transgene was successfully detected using Basta spray from the non-transgenic plants bearing the transgene introduced by hand-pollination. Since the Basta spray method developed in this study is easy to apply and economical, it will be a valuable tool for understanding the mechanism of gene flow through pollen transfer and for establishing a biosafety test protocol for genetically modified (GM) Chinese cabbage cultivars.

Effects of Ozone on $CO_2$ Assimilation and PSII Function in Two Tobacco Cultivars with Different Sensitivities

  • Yun, Myoung-Hui
    • Journal of Korean Society for Atmospheric Environment
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    • 제22권E2호
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    • pp.89-98
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    • 2006
  • Two tobacco cultivars (Nicotiana tabacum L.), Bel-B and Bel-W3, tolerant and sensitive to ozone, respectively, were grown in a greenhouse supplied with charcoal filtered air and exposed to 200 ppb ozone for 4 hr. Effects on chlorophyll fluorescence, net photosynthesis, and stomatal conductance are described. Quantum yield was calculated from chlorophyll fluorescence and the initial slope of the assimilation-light curve measured by the gas exchange method. Only the sensitive cultivar, Bel-W3, developed visual injury symptoms on up to 50% of the $5^{th}$ leaf. The maximum net photosynthetic rate of ozone-treated plants was reduced 40% compared to control plants immediately after ozone fumigation in the tolerant cultivar; however, photosynthesis recovered by 24 hr post fumigation and remained at the same level as control plants. On the other hand, ozone exposure reduced maximum net photosynthesis up to 50%, with no recovery, in the sensitive cultivar apparently causing permanent damage to the photosystem. Reductions in apparent quantum efficiency, calculated from the assimilation-light curve, differed between cultivars. Bel-B showed an immediate depression of 14% compared to controls, whereas, Bel-W3 showed a 27% decline. Electron transport rate (ETR), at saturating light intensity, decreased 58% and 80% immediately after ozone treatment in Bel-B and Bel-W3, respectively. Quantum yield decreased 28% and 36% in Bel-B and Bel-W3, respectively. It can be concluded that ozone caused a greater relative decrease in linear electron transport than maximum net photosynthesis, suggesting greater damage to PSII than the carbon reduction cycle.

EFFECT OF EXPERIMENTAL INFECll0N WITH STOMACH WORM (Haemonchus contortus) ON THE PRODUCTIVE PERFORMANCE OF DOES

  • Howlader, M.M.R.;Capitan, S.S.;Eduardo, S.L.;Sevilla, C.C.;Roxas, N.P.
    • Asian-Australasian Journal of Animal Sciences
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    • 제9권4호
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    • pp.421-426
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    • 1996
  • Comparison of body weight changes of does infected with Haemonchus contortus (treatment groups 2 &3) and uninfected does (control group) was made using weekly body weight measurements over 35 weeks. The animals in treatment group 2 weighted significantly (p < 0.05) less than animals in the control group from the 5th week to the end of the experiment. Does in treatment group 3 had significantly (p < 0.05) lighter body weight than control does from the 13th week to the end of the study. The animals in control group gained an average of 0.75 kg. On the other hand, animals in infected groups 2 and 3 lost an average of 3.90 kg and 4.13 kg body weight, respectively. The animals in groups 2 and 3 also had significantly (p < 0.05) lower preslaughter and hot carcass weights than the controls.

재활심리분석시스템의 다중 우회기반 접근통제 모델 및 응용 (Indirection based Multilevel Security Model and Application of Rehabilitation Psychology Analysis System)

  • 김영수;조선구
    • 한국정보통신학회논문지
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    • 제17권10호
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    • pp.2301-2308
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    • 2013
  • 재활심리분석시스템으로서 웹이 보편적으로 사용되면서 스팸메시지가 급격히 증가하고 있다. 이의 억제를 위한 메시지통제시스템은 기술적이고 경제적 대안으로 필터시스템과 과금 시스템을 사용하고 있으나 차단오류와 과금저항으로 인한 메시지의 수신과 가용성을 제한한다. 이의 해결을 위해서 불법적인 메시지는 억제하지만 송 수신자에게 유익하고 합법적인 메시지가 폐기되고 상실되는 것을 방지할 수 있는 메시지통제시스템의 우회 모델을 제안하고 이를 사용하여 메시지의 유용성과 가용성을 높여줄 수 있는 웹기반의 메시지통제시스템을 구현하여 모델의 실용성을 검증하였다.

비예정 재입원의 위험요인에 대한 환자-대조군 연구 (A Case-control Study of unexpected Readmission in a University Hospital)

  • 유승흠;오현주
    • Journal of Preventive Medicine and Public Health
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    • 제32권3호
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    • pp.289-296
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    • 1999
  • Objectives: This study describes the risk factors affecting the unexpected readmission of 261 patients who were discharged from a university hospital in Seoul. Methods: This case-control study reviewed medical records of inpatients who had been discharged from a hospital between 1 August 1995 and 31 October 1995 after the treatment for general diseases. The cases were 68 patients who were readmitted unexpectedly within 28 days of discharge from an index stay, and the controls were 193 Patients who were discharged without readmission during the study period. Results: Logistic regression analysis results were as follows; Patients who had no operation during their hospital stay were more likely to be readmitted unexpectedly than patients who had operation. Patients who had 1 or 2 parts of their body being involved in treatment were more likely to be readmitted unexpectedly than patients who hand more than 3 parts of their body being involved in treatment. Patients who had complications after surgery were more likely to be readmitted unexpectedly than patients who had no complications. Insufficient discharge planning caused unexpected readmissions. Conclusions: Discharge planning education should be extended to health care providers. And the assessment of discharge planning should be evaluated. Readmission is often necessary for the treatment of related problems of originating from initial hospitalization, which causes cost problems. Unexpected readmission is preventable and the models for readmission can serve as a valuable clinical tool for high risk patients.

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승용차량 구동축의 작용력에 따른 진동특성 연구 (A Study on the Characteristics of Vibration Due to the Forces of Drive Shaft)

  • 사종성;강태원
    • 한국생산제조학회지
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    • 제22권4호
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    • pp.708-716
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    • 2013
  • This study aims to understand the applied forces and related vibrational characteristics of a tripod joint (TJ), which is mostly used in front-drive-type middle-sized sedans in South Korea. The plunging force (PF) and generated axial force (GAF) are the most influential quantities related to the vibrational characteristics of a driveshaft. To obtain meaningful data, specially designed tests were performed using MTS test sets. The results of direct measurements reveal that higher PF and GAF values appear to worsen the vibrational characteristics of the vehicle. On the other hand, the measured apparent mass is useful for calculating the applied forces for a short driveshaft that has no dynamic vibration absorber. Among diversely controlled samples, it shows that the viscosity and tight fit are very sensitive to shudder vibrations of the vehicle. Therefore, these are good design factors for quality controls in the production line of constant-velocity joints.

영상처리를 이용한 지화인식 기반의 차세대 인터페이스 시스템 개발 (A Development of the Next-generation Interface System Based on the Finger Gesture Recognizing in Use of Image Process Techniques)

  • 김남호
    • 한국정보통신학회논문지
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    • 제15권4호
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    • pp.935-942
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    • 2011
  • 본 연구는 카메라를 통하여 취득한 지화를 자동으로 인식하고, 컴퓨터를 제어하는 지화인식 시스템을 설계하고 구현하는데 목적이 있다. 먼저 영상을 취득하기 위하여 일반 카메라를 개조하여 적외선 CCD 카메라를 제작하였고, 입력영상의 전처리와 손의 특징들을 찾아 분석하여 손 모양에 따라 해당되는 지화를 판독하고, 이벤트를 발생시켜 마우스 제어와 프리젠테이션을 수행할 수 있는 방법을 제시하였다. 본 연구에서 제안하는 지화인식 시스템은 미래형 정보기기의 마우스와 키보드를 대체할 수 있는 차세대 인터페이스로서의 사용가능성을 검증하였다.

Case-Control Study of Diet and Prostate Cancer in a Rural Population of Faisalabad, Pakistan

  • Bashir, Muhammad Naeem;Malik, Muhammad Akram
    • Asian Pacific Journal of Cancer Prevention
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    • 제16권6호
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    • pp.2375-2378
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    • 2015
  • Background: The effects of diet on epidemiology of prostate cancer are inconclusive. Therefore a hospitalbased, case-control study was conducted in a rural population of Faisalabad, Pakistan, to examine the impact of dietary factors on risk of cancer development. Materials and Methods: This study was based on 102 confirmed cases of prostate cancer and 204 normal controls. Logistic regression was used to estimate odds ratios and 95% confidence intervals for odds ratios to evaluate the relationship between prostate cancer and diet. Results: Consumption of red meat and fat items significantly increased the prostate cancer risk having odds ratios and 95% confidence intervals of 3.41; 1.46-7.96 and 2.45; 1.17-5.15, respectively. On the other hand, more consumption of vegetables, fluid intake and fruit significantly decreased the prostate cancer risk (odd ratios and corresponding 95% confidence intervals of 0.21; 0.10-0.44, 0.10; 0.05- 0.19 and 0.09; 0.03- 0.23, respectively. Conclusions: The present study supports the hypothesis that frequent consumption of red meat and fat items may increase prostate cancer risk while more intake of fruit, vegetables and fluid intake may protect against prostate cancer in the relatively low risk group in rural Pakistan.

Risk Factors of Prostate Cancer: a Case-control Study in Faisalabad, Pakistan

  • Bashir, Muhammad Naeem;Ahmad, Muhammad Riaz;Malik, Akram
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권23호
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    • pp.10237-10240
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    • 2015
  • Background: Prostate cancer is the third most commonly diagnosed cancer among males in Pakistan but very little is known about risk factors among the Pakistani population. Therefore a hospital-based, case-control study was carried out in Faisalabad to identify potential risk factors. Materials and Methods: This study was based on 140 prostate cancer cases and 280 normal controls. Logistic regression was used to estimate odds ratios and 95% confidence intervals for odds ratios to assess the relationship between prostate cancer and different risk factors. Results: Family history of prostate cancer, age, smoking, obesity, consumption of red meat and frequent use of fat items significantly increased the prostate cancer risk (odds ratios and 95% confidence intervals of: 7.32; 1.79-29.8; 16.9, 5.60-50.8; 2.47, 1.17-5.18; 5.79, 2.66-12.6; 2.71, 1.07-6.91; and 3.39, 1.47-7.83, respectively. On the other hand, more consumption of fruit, fluid intake and better lifestyle (physical activity) significantly reduced the risk of developing prostate cancer with odd ratios and corresponding 95% confidence intervals of: 0.27, 0.11-0.61; 0.05, 0.02-0.12; and 0.28, 0.13-0.58. Conclusions: The results of the present study suggested that age, family history of prostate cancer, smoking, obesity, fluid intake, frequent use of fat items, consumption of fruits and better lifestyle might be associated with prostate cancer among Pakistani males.

사람에서 n-3계 불포화지방산이 Serum Lipoprotein과 지질조성에 미치는 영향 (Effect n-3 Polyunsaturated Fatty Acids on Serum Lipoprotein and Lipid Compositions in Human Subjects)

  • 박현서
    • Journal of Nutrition and Health
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    • 제21권1호
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    • pp.61-74
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    • 1988
  • Ten college women were divided into 5 groups and treated in randomized block design for 5 weeks with 1 interval between treatments and subjects serving as their own controls. The experimental diets were corn oil diet as a source of n-6 linoleic acid, perilla oil diet as a source of n-3 $\alpha$-linolenic acid, and fish oil diet as a source of n-3 EPA and DHA. Dietary fat was supplied at 30% Cal and modified to give the total amount of saturated fatty acids and monoenoic acids at constant level. There was no significant effect on serum cholesterol level by different PUFA. However, on a gram-for-gram basis, there was a trend that the decrease in serum cholesterol was proportionate to the degree of fat unsaturation. On the other hand, only fish oil diet significantly decreased TG level but no significant effect on the relative proportion of TG in VLDL. The degree of hypotriglyceridemia did not corrleate with the degree of unsaturation. The relative proportion of CE in LDL was reduced by all PUFA diets but significant only by perilla oil diet. The relative amount of apoprotein in LDL was significantly reduced by n-3 PUFA. HDL-Chol content was significantly increased only in fish oil diet but no change in the relative proportion of its chemical components of HDL.

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