• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.74-74
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• 2003

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.10a
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• pp.323-324
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• 2001

전복류는 전세계적으로 대략 100여종이 북반구와 남반구에서 넓게 분포 서식하고 있으며, 이 중 대략 10여종이 현재 상업적으로 유용하다. 한국연안에는 한류계인 참전복, Haliotis discus hannai Ino과 난류계인 까막전복, 시볼트전복, 말전복, 오분자기 및 마대오분자기가 있으며, 이중 참전복은 제주도를 제외한 전 연안에 걸쳐서 분포하며 현재 대표되는 양식종이다. (중략)

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.317-322
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• 2011

This study was performed to obtain the basic data on physiological responses of low water temperature stress of the cultured Pacific abalone, Haliotis discus hannai. Abalones were exposed at low water temperatures of $7^{\circ}C$ and $4^{\circ}C$. We have investigated survival rate, superoxide dismutase (SOD) activity and total protein (TP) in the abalone by the exposure times (0, 3, 6, 12, 24, 72, 120, 168 and 216 hours). Survival rate of the abalone at $7^{\circ}C$ experiment was 90.8%, whereas at $4^{\circ}C$ experiment was 0% after exposure 10 days. SOD activity was significantly increased until 12 hours after exposure to $4^{\circ}C$, and then was recovered to starting level after 24 hours. However, there was no significant difference between control ($12^{\circ}C$) and $7^{\circ}C$ experiments. TP was significantly increased until 216 hours after 24 hours at $4^{\circ}C$ experiment, but $7^{\circ}C$ experiment showed no significant differences compared to control ($12^{\circ}C$) experiment. Therefore, H. discus hannai was acclimated in low water temperature stress at $7^{\circ}C$, but at $4^{\circ}C$, all abalone died possibly because they exceed the limits of defense ability to too low temperature.

• The Korean Journal of Malacology
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• v.30 no.4
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• pp.415-419
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• 2014

Inbreeding depression may be an avoidable phenomenon for abalone culture. However, only a few studies were carried out on inbreeding depression. In the present study, to demonstrate inbreeding depression in growth trait of Pacific abalone, H. discus hannai, inbreeding and outbreeding families were produced in 2010. Inbred and outbred families from each experiment were reared in same tank until 10 month for the same breed environment. The individual of inbred and outbred were distinguished by paternity test using microsatellite DNA. The shell length between inbred and outbred families was compared. At the results, significantly higher shell length was observed in the outbred families at 10 mon (P < 0.05). These results indicate that inbreeding depression is obviously observed in growth traits in the first generation of full-sib family of the H. discus hannai.

• The Korean Journal of Malacology
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• v.29 no.3
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• pp.197-205
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• 2013

Even though seedling production of Haliotis discus hannai has fully developed, the culture of benthic diatom as a live food for larvae is still a barrier to solve in commercial hatchery. The farmer depends on mixed microalgae which are non-selectively attached on the plate by flowing of natural seawater. The adequate diatom on the plate for the larvae in terms of quality and quantity is always significant bottleneck in the hatchery. In this study, two benthic diatom species, Caloneis schroederi and Rhaphoneis sp. were separately cultured in mass and inoculated to four tons' settlement tank of the larvae. And the larvae and the spats were cultured for nineteen and nine weeks, respectively. The result on seedling production of H. discus hannai with this method was compared to that of the farmer's traditional method as a control. With regard to variation of species composition of benthic diatom on the plate, C. schroederi and Rhaphoneis sp. were dominant for first three weeks after inoculation. But the diverse diatoms mainly, Navicula, Amphora, Cylindrotheca, Licmophora, Pleurosigma began to attache on the plate from the 4th week. The larvae attached 2.5 times more in C. schroederi tank than in the control tank. The final total biomass of the seeds in Rhaphoneis sp. tank was 3.2 times more than that of the control tank. The retared-spats in the seedling production also showed significantly higher growth and survival in the spat fed Rhaphoneis sp. or C. schroederi than those in the control group. We suggest that C. schroederi is proper for settlement of the larvae and Rhaphoneis sp. is appropriate for the growth of the larvae and spats in the commercial hatchery of H. discus hannai.

• The Korean Journal of Malacology
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• v.29 no.2
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• pp.91-96
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• 2013

Although the method of seedling production of Haliotis discus hannai is well known, the optimum benthic diatom species as a live food at early larval stage are not fully developed. In this study three Pennales diatom species, Caloneis schroederi, Rhaphoneis sp., and Cocconeis californica were examined on settlement, metamorphosis, survival, and growth of Haliotis discus hannai larvae. The larvae fed Raphoneis sp. or C. californica showed high settlement rate with 80-82% within 48 hrs, which was significantly higher than those fed C. schroederi or mixed diets with three diatom species. The larvae fed the former microalgal species also showed higher metamorphosis rate with 32-34% than the latter species with 10-12% within 4 days. With regard to survival and growth of the larvae, single diet with Rhaphoneis sp. or C. californica had better dietary value than the mixed diets for the early larvae of H. discus hannai.

• Journal of Life Science
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• v.24 no.12
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• pp.1291-1300
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• 2014

Peroxiredoxins (Prxs) are a ubiquitous family of antioxidant enzymes that participate in a variety of biological processes, including $H_2O_2$-mediated signal transduction, molecular chaperoning, and mitochondrial function. In this study, we isolated and characterized a Prx 2 cDNA from abalone (Haliotis discus hannai). The abalone Prx 2 cDNA encoded a 199-amino acid polypeptide that belongs to a class of typical 2-Cys Prxs that contain peroxidatic and resolving cysteines. The deduced abalone Prx 2 protein showed strong homology (64-99%) with Prx 2 proteins from other species, including mollusk, fish, amphibians, and mammals, and it was most closely related to disk abalone (H. discus discus) Prx 2. Abalone Prx 2 mRNA was ubiquitously detected in tested tissues, and its expression was comparatively high in the mantle, gills, liver, foot, and digestive duct. The expression level of abalone Prx 2 mRNA was 106.7-fold, 51.9-fold, and 437.8-fold higher, respectively, in the gills, digestive duct, and liver than in the muscles. The expression level of abalone Prx 2 mRNA in the liver peaked at 6 hr postinfection with Vibrio parahemolyticus and decreased at 12 hr postinfection. The expression level of abalone Prx 2 mRNA in hemocytes was drastically increased at 1 hr postinfection with V. parahemolyticus. These results suggest that abalone Prx 2 is conserved through evolution and that it may play a role similar to that of its mammalian counterpart.

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.281-282
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• 2006

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.397-398
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• 2006

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.395-396
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• 2006