• Molecules and Cells
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• 제24권2호
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• pp.210-214
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• 2007

The 90-kDa heat shock protein (HSP90) normally functions as a molecular chaperone participating in folding and stabilizing newly synthesized proteins, and refolding denatured proteins. The HSP90 inhibitor geldanamycin (GA) occupies the ATP/ADP binding pocket of HSP90 so inhibits its chaperone activity and causes subsequent degradation of HSP90 client proteins by proteasomes. Here we show that GA reduces the level of endogenous c-Jun in human embryonic kidney 293 (HEK293) cells in a time and dose dependent manner, and that this decrease can be reversed by transfection of HSP90 plasmids. Transfection of HSP90 plasmids in the absence of GA increases the level of endogenous c-Jun protein, but has no obvious affect on c-Jun mRNA levels. We also showed that HSP90 prolongs the half-life of c-Jun by stabilizing the protein; the proteasome inhibitor N-benzoyloxycarbonyl (Z)-Leu-Leu-leucinal (MG132) blocks the degradation of c-Jun promoted by GA. Transfection of HSP90 plasmids did not obviously alter phosphorylation of c-Jun, and a Jun-2 luciferase activity assay indicated that over-expression of HSP90 elevated the total protein activity of c-Jun in HEK293 cells. All our evidence indicates that HSP90 stabilizes c-Jun protein, and so increases the total activity of c-Jun in HEK293 cells.

• 한국어병학회지
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• 제34권2호
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• pp.255-259
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• 2021

This is the first report describing acute mass mortality occurred in juvenile olive flounder (Paralichthys olivaceus) caused by gas bubble disease (GBD). A total of 610 fish (average weight = 35 g), which were more than half of the fish acclimated at 17℃ in an aquarium, were killed within two days of acclimation. The dead and moribund fish showed excessively opened opercula and mouths, and occasionally, severe exophthalmia. Through microscopic observation, numerous gas emboli were found in the gills of the dead and live fish, while the fish were not infected with any microbial pathogens. The dissolved oxygen (DO) saturation level of the rearing water and seawater nearby the facility reached 145% and 286%, respectively, whereas other water quality parameters (such as salinity, pH, and chemical oxygen demand) were normal. The extreme saturation rate of seawater in the shore nearby seemed to be due to an enormous algal bloom that occurred there. Through molecular identification based on 18S rDNA sequences, the most dominant algal species was most closely related to Ulva californica (99.87% sequence identity) followed by U. prolifera, U. linza, and U. curvata (99.81%). Therefore, it can be concluded that supersaturated seawater due to mass algal bloom caused gas bubble disease in the olive flounder, leading to mass mortality. After technical adjustment, such as increased aeration, lowered water circulation rate, and inlet water filtration using micro-pore carbon filters, the DO level became normal, no further mortality occurred and the status of the fish was stabilized.

• Molecules and Cells
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• 제44권2호
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• pp.101-115
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• 2021

The INO80 chromatin remodeling complex has roles in many essential cellular processes, including DNA replication. However, the mechanisms that regulate INO80 in these processes remain largely unknown. We previously reported that the stability of Ino80, the catalytic ATPase subunit of INO80, is regulated by the ubiquitin proteasome system and that BRCA1-associated protein-1 (BAP1), a nuclear deubiquitinase with tumor suppressor activity, stabilizes Ino80 via deubiquitination and promotes replication fork progression. However, the E3 ubiquitin ligase that targets Ino80 for proteasomal degradation was unknown. Here, we identified the C-terminus of Hsp70-interacting protein (CHIP), the E3 ubiquitin ligase that functions in cooperation with Hsp70, as an Ino80-interacting protein. CHIP polyubiquitinates Ino80 in a manner dependent on Hsp70. Contrary to our expectation that CHIP degrades Ino80, CHIP instead stabilizes Ino80 by extending its half-life. The data suggest that CHIP stabilizes Ino80 by inhibiting degradative ubiquitination. We also show that CHIP works together with BAP1 to enhance the stabilization of Ino80, leading to its chromatin binding. Interestingly, both depletion and overexpression of CHIP compromise replication fork progression with little effect on fork stalling, as similarly observed for BAP1 and Ino80, indicating that an optimal cellular level of Ino80 is important for replication fork speed but not for replication stress suppression. This work therefore idenitifes CHIP as an E3 ubiquitin ligase that stabilizes Ino80 via nondegradative ubiquitination and suggests that CHIP and BAP1 act in concert to regulate Ino80 ubiquitination to fine-tune its stability for efficient DNA replication.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.225-225
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• 2022

Rice is a major food crop consumed by approximately half of the world's population. Heading date is one of the major agronomic traits and has a wide impact on the productivity and quality of rice. Recently, shortening the growth period of rice through modulating heading date has been emphasized as one of the most effective strategies for reducing methane emissions from paddy fields. OsPRR37, a major heading date gene in rice, represses flowering under both short-day and long-day conditions. Plants carrying the loss-of-function alleles of OsPRR37 have been reported to flower approximately seven days and 20 days earlier than those carrying the functional alleles in short day and long day conditions, respectively. In this study, we investigated the nucleotide sequence variation existing in the exonic regions of OsPRR37 and catalogued the allelic distribution in 208 Korean rice cultivars. We used four sets of primers for amplifying and sequencing the eight exons of OsPRR37. As a result, two types of loss-of-function alleles and four types of functional alleles were found in 208 Korean rice cultivars. Interestingly, only three cultivars (Jinbuolbyeo, Jinseolchal, and Mimyeon) carried loss-of-function alleles while 205 carried functional alleles, indicating that OsPRR37 loss-of-function alleles have been used very rarely in Korean rice breeding programs. To generate useful information for the development of early-maturing rice cultivars, our future work will focus on analyzing the effect of different OsPRR37 alleles on heading date and other major agronomic traits.

• Journal of Contemporary Eastern Asia
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• 제12권2호
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• pp.49-59
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• 2013

Social media such as Twitter and Facebook have become popular. In the age of social media, many people have become more active online. For example, about half of all global active Internet users are on Facebook (Perry 2012). Personal branding is a very important strategy to build on an individual's strengths, and this kind of branding is expected to contribute to self-actualization. Therefore, the presence of mentors who advise users to discover their own strong points for self-actualization is necessary. In this paper, we propose a personal branding support service, Mentors, which connects mentors and mentees. The core concept is: "Everyone has the face of both a mentor and mentee." The key function is sharing stages of self-analysis in human life design: Determining value, creating a mission, and forming a strategy. From this function, a good match between a mentor and mentee can be found. The program aims to improve human life by understanding the client's strengths and using social media effectively. Future work includes launching Mentors and evaluating its service.

• International Journal of Advanced Culture Technology
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• 제8권1호
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• pp.218-225
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• 2020

Purpose Wearable devices are widely used in our daily life. The purpose of this study is to investigate the relationship between persuasive designs of fitness trackers and users' physical activity behavior. Methods To test the research model, data was collected from a web-based survey in China, resulting in an effective sample of 166 usable questionnaires. The survey was restricted only to respondents who wear a fitness tracker. Results The sample surveyed in this study indicated that half of the respondents had been wearing a smart fitness tracker shorter than one year, and only 27% were long-time users (longer than two years). Dialogue support and social support strategies were both proved to be effective in increasing users' workout behavior intention. Social support strategies had a greater effect on behavior change than dialogue support strategies. Conclusion The findings from this study make several contributions to the practice. Wearable devices developers can employ the result from this study to help them design devices, which can persuade people to do more exercises and preserve a healthier life.

• 펄프종이기술
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• 제42권3호
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• pp.14-21
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• 2010

Three different species of cattails (Typha orientalis, Typha angustata, Typha latifolia) cultivated in South Korea were examined as raw materials for the production of pulp and paper. Soda pulping with anthraquinone was used for the stems and the leaves of cattail, separately. Addition of anthraquinone in soda pulping improved the yield greatly. Chemical components between three cattail species and within each species (leaves and stem) gave little differences. Average fiber length of the bleached cattail fibers was a little shorter than that of the HwBKP of Eucalyptus. Average fiber width of the cattail fibers was a half of that of the HwBKP. Compared to the HwBKP, cattail pulp gave higher breaking length, higher opacity, and better smoothness at equivalent freeness.

• 가정과삶의질연구
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• 제11권1호
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• pp.35-45
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• 1993

The purpose of this study is to investigate the changes of housing characteristics and the degree of attainment of housing norms. The major findings are as follows: 1) There are significant differences in changes of housing characteristics. A size of house and the number of room have been improved according to hosing experience and housing expects. Type of tenure has been improved according to housing experience and housing expects. Type of tenure has been improved gradually according to moving. The family with higher socio-economic status prefers apartments. Although about a half of household hopes to live in a high-rising apartments. more than 40 persentage of the household still hopes to live in single detached houses. As a result apartment are though not to satisfy their housing well-being 2) As socio-economic status is higher and family life cycle is longer the degree of their attainment of housing norms appear higher. The household attaining higher housing norms has the higher housing satisfaction but needs to move is lower The family attains housing norms as the following orders: type of tenure\longrightarrowtype of house\longrightarrowthe nmber of rooms\longrightarrowa size of house.

• 한국기계가공학회지
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• 제14권3호
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• pp.92-98
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• 2015

This study investigates the durability of a two-passenger bicycle frame under non-uniform fatigue load. The bicycle frame of Model 1 installed with reinforcement support has a 20% lower maximum equivalent stress than the existing Model 2. Model 1 has a maximum total deformation that is less than half that of Model 2. Model 1 has a higher maximum fatigue life than Model 2. In addition, Model 1 has lower fatigue damage than Model 2. Thus, the bicycle frame of Model 1 installed with reinforcement support can be described as safer, as it offers more strength than Model 2. Applying this result to the design of a real two-passenger bicycle frame under non-uniform fatigue load can effectively prevent fatigue damage and improve durability.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2015년도 제46회 하계학술대회
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• pp.968-969
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• 2015

This paper proposes a novel analysis method for calculating inverter DC bus capacitance and line reactor parameters. In the realization process, DC bus capacitance parameter, and ripple current, life of DC bus capacitor, interaction between DC bus capacitance can be calculated by using Newton-Raphson procedure. The design scheme of DC bus capacitor and line reactor, specific parameters such as capacitance, loss, ripple current, central average temperature, life, ripple current, loss, size, central temperature of the reactor were given. Simulation results show that this scheme can accurately calculate the DC bus capacitance and line reactor parameters. Compared with calculation result of references, cost and volume are half. The indicators meet the demand of practical engineering. It had affirmed precision of the analytical method and verified correctness and feasibility of this method.