• Title/Summary/Keyword: Ha29

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Characterization of Ha29, a Specific Gene for Helicoverpa armigera Single-nucleocapsid Nucleopolyhedrovirus

  • Guo, Zhong-Jian;An, Shi-Heng;Wang, Dun;Liu, Yan-He;Kumar, V. Shyam;Zhang, Chuan-Xi
    • BMB Reports
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    • v.38 no.3
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    • pp.354-359
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    • 2005
  • Open reading frame 29 (ha29) is a gene specific for Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearSNPV). Sequence analyses showed that the transcription factor Tfb2 motif, bromodomain and Half-A-TPR (HAT) repeat were present at aa 66-82, 4-76, 55-90 of the Ha29 protein respectively. The product of Ha29 was detected in HearSNPV-infected HzAM1 cells at 3 h post-infection. Western blot analysis using a polyclonal antibody produced by immunizing a rabbit with purified GST-Ha29 fusion protein indicates that Ha29 is an early gene. The size of Ha29 product in infected HzAM1 cells was about 25 kDa, which was larger than the presumed size of 20.4 kDa. Tunicamycin treatment of HearSNPV-infected HzAM1 cells suggested that the Ha29 protein is N-glycosylated. Fluorescent confocal laser scanning microscope examination, and Western blot analysis of purified budded virus (BVs), occlusion-derived virus (ODVs), cell nuclear and cytoplasmic fraction, showed that the Ha29 protein was localized in the nucleus. Our results suggested that ha29 of HearSNPV encodes a non-structurally functional protein that may be associated with virus gene transcription in Helicoverpa hosts.

Cone Production Increase by Pruning Treatment in Pinus koraiensis Seed Orchard (잣나무 수형조절에 의한 구과생산 증진)

  • 유세걸;김종한
    • Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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    • 2001.06a
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    • pp.81-84
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    • 2001
  • 잣나무는 지난 1990-1999년(10년간)까지 83,422ha(년간 8,342ha)의 면적에 조림을 실시하여 같은 기간(10년간)의 전국 총조림면적 283,071ha(년간 28,307ha)의 약 29%를 차지하였다. 임업연구원에서는 1968년부터 1983년까지 91ha(동부육종장 69ha, 중부육종장22ha)의 채종원을 조성하여 1976년부터 2000년까지 62,810kg(조림가능면적 : 6,281ha)의 우량종자를 생산보급하였으며 수령의 증가에 따라 매년 종자 생산량은 계속 증가하고 있다.(중략)

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Annual Runoff Loading of Nitrogen and Phosphorus from a Paddy Field

  • Han, Kang-Wan;Cho, Jae-Young;Choi, Jin-Kyu
    • Journal of Applied Biological Chemistry
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    • v.42 no.1
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    • pp.29-33
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    • 1999
  • The present study examined annual runoff loading of nitrogen and phosphorus in the paddy field from 1 May, 1997 to 30 April, 1998. In the investigated area, the amount of rainfall was 1,095.6 mm and 414.6 mm during cropping season and non-cropping season. The annual rainfall was 1,510.2 mm. The total amount of runoff water was 1,043.2 mm and 281.0mm during cropping season and non-cropping season, and the added total amount of runoff water during two seasons was 1,324.2 mm. The runoff loading of nutrients caused by runoff water was measured as follows. The total-N was 149.23 and $8.67kg\;ha^{-1}$ (total amount=$157.90kg^{-1}ha^{-1}yr^{-1}$), the ammonia-N 102.98 and $4.44kg\;ha^{-1}$ ($107.42kg^{-1}ha^{-1}yr^{-1}$), the nitrate-N 28.45 and $1.23kg\;ha^{-1}$ ($29.68kg^{-1}ha^{-1}yr^{-1}$), the total-P 4.16 and $0.38kg\;ha^{-1}$ ($4.54kg^{-1}ha^{-1}yr^{-1}$) during cropping and non-cropping season respectively. When the loss ratio was calculated based on amounts of chemical fertilizer, about 68.6% of nitrogen and 16.7% of phosphorus was lost by runoff from applied fertilizer amount.

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Home-range of the Common Kestrel (Falco tinnunculus) in the Suburban Area of Busan

  • Kang, Seung-Gu;Hur, Wee-Haeng;Lee, In-Sup
    • Korean Journal of Environment and Ecology
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    • v.29 no.2
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    • pp.162-173
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    • 2015
  • The current study analyzed the home range size of the Common Kestrel (Falco tinnunculus) by observation using the natural markings in the suburban area of Busan from December 2009 to May 2010. In all, 26 individual kestrels were observed in the study area, of which 14 individual kestrels were frequently observed (more than five times) and 12 were observed less frequently (less than five times). Mean home range sizes of the 14 frequently observed kestrels were estimated using two different methods (minimum convex polygon (MCP) method and fixed kernel (FK) method): 34.49 ha(2.74-163.96 ha) by 100% MCP, 41.35 ha(3.54-99.02 ha) by the 95% FK, 14.33 ha(0.20-31.57 ha) by the 75% FK and 5.75 ha(0.52-14.74 ha) by the 50% FK. The mean home range size was larger for males than for females estimated at 51.50 ha(15.04-163.96 ha) and 11.80ha(2.74-24.48 ha), respectively, by the 100 % MCP method and 8.02 ha(2.38-14.74 ha) and 2.72 ha (0.52-4.28 ha), respectively, by the 50 % Fixed Kernel method. The home range sizes by sexes in breeding pairs differed during the wintering and breeding seasons; the sizes decreased among females but increased among males during the breeding season. Such differences of home range sizes between sexes could be attributed to parental role division during the breeding season. Females stay in the nest or next to it during laying, incubating, and feeding activities, and males go far away from their nest sites to forage areas for their fledglings for most of the breeding period.

Development of Allometric Equations for V Age-class Pinus koraiensis in Mt. Taehwa Plantation, Gyeonggi-do (경기도 태화산 V 영급 잣나무(Pinus koraiensis) 조림지의 지상부 바이오매스 상대생장식 개발)

  • Ryu, Daun;Moon, Minkyu;Park, Juhan;Cho, Sungsik;Kim, Taekyu;Kim, Hyun Seok
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.16 no.1
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    • pp.29-38
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    • 2014
  • Allometric equations for leaf, branch, stem and total above ground biomass of Pinus koraeinsis trees were developed with diameter at breast height(DBH) of trees, which were growing in a pine plantation with the stand density of 410 tree $ha^{-1}$ and the average DBH of $29.1{\pm}5.2$ cm in Mt. Taewha, Gyeonggi. Damage by Acantholyda parki reduced leaf biomass compared to other studies, however, its contribution to total biomass was minimal among parts. Comprehensive analysis revealed that constant in allometric equation for total above ground biomass (logY=a + blogX) was affected by average DBH and stand density, however, constant b was not. At the stand level, biomass for leaf, brach, stem, total above ground biomass were 6.68 Mg $ha^{-1}$, 18.82 Mg $ha^{-1}$, 101.02 Mg $ha^{-1}$, 126.53 Mg $ha^{-1}$, respectively. We developed a Korean pine stand biomass regression, which explained about 98% of variation with DBH and stand density based on comprehensive analysis.

Comparison of Growth, Yield and Yield Components among Rice Cultivars for Organic Farming in No-tillage Paddy

  • Son, Daniel;Lee, Young-Han
    • Korean Journal of Environmental Agriculture
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    • v.29 no.1
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    • pp.1-6
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    • 2010
  • Organic farming system in rice paddy is rapidly expanding in Korea. This study was to find out optimum japonica rice cultivars for organic farming. A field research was conducted to evaluate the characteristics of japonica rice cultivars under no-tillage paddy at Doo-ryangmyeon, Sacheon, Gyeongsangnam-do, Korea. The experimental soil was Juggog series (silty clay loam: 56.0% silt, 31.2% clay and 12.8% sand). In experiment, ten lines of Japanese rice cultivars were tested under no-tillage amended with rye (NTR) and no-tillage without cover crop treatment (NTNT). In addition, two Korean japonica rice cultivars as check cultivars were used in this study. The grain yield in NTR was significantly higher in 6.13 Mg $ha^{-1}$ for Kinuhikari, 5.30 Mg $ha^{-1}$ for Komekogane, 5.25 Mg $ha^{-1}$ for Kosihikari, 5.22 Mg $ha^{-1}$ for Mazizbare and 5.12 Mg $ha^{-1}$ for Akitakomachi compared to two Korean rice cultivars (4.57 Mg $ha^{-1}$ for Hwayoungbyeo and 4.00 Mg $ha^{-1}$ for Ilmibyeo) in that order. While, grain yield in NTNT was significantly higher in 4.90 Mg $ha^{-1}$ for Akitakomachi 3.81 Mg $ha^{-1}$ for Hinohikari, 3.74 Mg $ha^{-1}$ for Umezkusi, 3.67 Mg $ha^{-1}$ for Kosihikari and 3.54 Mg $ha^{-1}$ for Dondokuri compared to 3.02 Mg $ha^{-1}$ for Ilmibyeo and 2.36 Mg $ha^{-1}$ for Hwayoungbyeo, respectively. The number of panicle per $m^2$ and grain number per panicle were indispensible for increasing the yield of rice. These results were able to find out optimum japonica rice cultivar Akitakomachi for organic farming in no-tillage paddy.

Ecological Role of Earthworms in Red Pine Forest in the Limestone Areas (석회암지역 소나무림에서 지렁이의 생태학적 역할)

  • Mun, Hyeong-Tae;Jeong-Ok In
    • The Korean Journal of Ecology
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    • v.16 no.2
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    • pp.229-238
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    • 1993
  • Ecological role of earthworms in red pine forest was investigated with emphasis on the population size, cast production and changes of physicoKDICical properties of soil by earthworm's activities. The density of earthworms was 62.8/m2 in August, 1992. Average biomass of earthworms was 16.3 g/m2. N and P concentrations in the tissue of earthworms were 28.5 mgN/g and 0.5 mgP/g, respectively. Cast production continued from April to November, with a peak in August. Annual production of earthworm casts was 5,379 g/m2. Bulk density of surface soil decreased due to the earthworm's cast-forming activity. Clay fraction in surface soil increased by addition of casts. Soil KDICical properties were significantly different between the surface soil and the earthworm casts. The amounts of organic matter, N, P, K, Ca and Mg added to the soil via earthworm casts during one year were 9.3t/ha, 11.29 kg/ha, 0.78 kg/ha, 12.36 kg/ha, 140.29 kg/ha and 20.96 kg/ha, respectively. Earthworms can feed on waxy and resinous litter when it became palatable by decomposing processes.

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