• Journal of Korean Society on Water Environment
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• v.24 no.6
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• pp.709-717
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• 2008

The field scale experiment which is constructed with four sets (0.88 ha for each set) of wetland (0.8 ha) and pond (0.08 ha) systems was performed to examine the effect of plant coverage on the constructed wetland performance and to recommend the optimum development and management of macrophyte communities. After six growing seasons of wetlands, plant coverage was about 100%. And the concentration of DO showed low value (1.0~5.4 mg/L). This is caused by a blighted plant consumed dissolved oxygen with decay in water column. As the result, water column went to be anaerobic conditions and T-N removal rate are 58~67%. Dead vegetation increased nitrogen removal during winter because it is a source of organic carbon which is an essential parameter in denitrification. However, wetland released phosphorus caused by a blighted plant and accumulation, the removal rate of phosphorus might be decreased. To rise of DO concentration, the three open-waters were constructed in cell 3 and 4. Cell 3 has two open-waters (width 10 m, depth 1.8 m) and cell 4 has one open-water (width 20 m, depth 1.8 m). As the result, DO concentration and treatment efficiency of nutrient and BOD were improved. In case that constructed wetland is operated for a long time, physical circulation structure such as open water help continuous circulation of aerobic and anaerobic conditions. Through the constructed open-water, treatment efficiency of phosphorus and nitrogen in wetland could be improved effectively.

• Food Science of Animal Resources
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• v.35 no.1
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• pp.58-70
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• 2015

This study was conducted to examine the effect of egg shell membrane hydrolysates (ESMH) on wrinkle, UV, and moisture protection for cosmetic use. ESMH were fragmented as whole ESMH (before fractioning), Fraction I (> 10 kDa), Fraction II (3-10 kDa), and Fraction III (< 3 kDa). In order to test whether fractionated ESMH can be used for functional cosmetic materials, we examined not only the level of hyaluronic acid and collagen production, but also the MMP-1 activity using a HaCaT and CCD-986Sk cell line. Our study treated each sample of fractionated ESMH with different concentrations (0.01, 0.1, 1 mg/mL). In our in vivo research, we used hairless mice that had been exposed to UV-B to induce wrinkles for 7 wk, then applied Fraction I to the treatment group for 5 wk and then tested skin thickness, minimum erythema dose and moisture content. In addition, Fraction I was high in collagen and HA biosynthesis and it was better than TGF-${\beta}$ in improving of the skin. When TNF-${\alpha}$ caused MMP-1 activity in the CCD-986Sk cells, the whole ESMH and Fraction I proved to be effective in hindering the induction of collagenase depending on the concentration, and also showed outstanding effects in the suppression of skin aging. We found that the treatment group mice's UV-B radiation-induced skin damage was largely mitigated compared to that of the non-treatment group mice. Thus, we have concluded that EMSH helps to mitigate UV-B radiation-induced wrinkles, collagen, HA, MMP-1 activity and can be used for functional cosmetic materials.

• The Journal of Pediatrics of Korean Medicine
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• v.20 no.3
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• pp.97-104
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• 2006

Objectives : There were few reports on Erysipelas, from Secondary Skin Infection after Herpes Zoster. We treated Erysipelas, from Secondary Skin Infection after Herpes Zoster with Oriental medical approach, and got a significant results. So We are reporting this case. Methods : Herb medication, acupuncture treatment were applied for treating patient`s chief symptoms (erythema, heat sensation, swelling, pain, itching and general weakness) Results : The patient was 5-year-old boy who had the symptoms caused by Erysipelas, which was caused by secondary skin infection after herpes zoster. He had erythema, heat sensation, swelling, pain, itching and general weakness. We judged him as Soyangin(少陽人), and prescribed Hyeongbangsaback-san. After he took Hyeongbangsaback-san, the symptom and general condition were improved. In this report, we want to explain the healing process and the result of the treatment of Erysipelas. Conclusions : More study about oriental treatment on Erysipelas, from Secondary Skin Infection after Herpes Zoster is needed.

• Biomolecules & Therapeutics
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• v.22 no.3
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• pp.260-266
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• 2014

This study evaluated the pharmacokinetic profile and therapeutic efficacy of piroxicam (PX), a long acting non-steroidal anti-inflammatory drug for the treatment of arthritis, following intra-articular (IA) injection in comparison to the pharmacokinetic profile and therapeutic efficacy of PX after intramuscular (IM) injection. In the pharmacokinetic study in rats, systemic exposure and pharmacokinetic parameters of PX after a single IA dose were compared with systemic exposure and pharmacokinetic parameters of PX after administration of the same dose IM (0.6 mg/kg). The anti-inflammatory and analgesic effects of IA PX were evaluated simultaneously in a monoiodoacetate-induced osteoarthritis rat model. The plasma PX concentration rapidly rose following IA injection, and it was comparable to the plasma PX concentration following IM injection, suggesting the rapid efflux of the drug molecule from the joint cavity. However, in the efficacy study, the IA PX administration significantly reduced the knee swelling by reducing the level of prostaglandin $E_2$ in the joint, compared to that following administration of IA vehicle and after administration of the IM PX dose. In addition, we found that the anti-inflammatory and anti-nociceptive efficacies of IA PX were synergistically increased upon co-treatment with hyaluronic acid (HA), a potent agent for the treatment of osteoarthritis, at the weight ratio of 1:1 or 1:2, and these effects were more pronounced than those following administration of HA or PX alone. In conclusion, this study demonstrated the efficacy of the IA use of PX alone and/or in combination with HA in osteoarthritis.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.2
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• pp.101-107
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• 2017

Corn is basal forage for livestock species in Republic of Korea but it lacks protein and needs nitrogenous fertilizer. This study was designed with main objective to achieve optimum growth, yield & nutritive value of forage for livestock through implementing corn-soybean intercropping strategy at 17 different places under Korean condition. Two treatments; corn as monocrop (control treatment) and corn-soybean intercrop were compared under Randomized Block Design from $28^{th}$ May to $8^{th}$ October, 2015. Each treatment had three replicates in each block, whereas seventeen different places were considered as blocks. Data were analyzed through SAS-9.1.3 software. Difference between two treatment means was tested through T-test. Findings depicted that intercropping pattern could not influence (P>0.05) corn plant & ear height, corn lodged stalk No. and corn stalks number. However, corn-soybean intercropping enhanced (P<0.05) forage productivity in terms of total fresh yield ($16.4{\pm}0.7^b$ vs. $19.9{\pm}0.7^atons\;ha^{-1}$), total dry matter yield ($5.38{\pm}0.25^b$ vs. $6.41{\pm}0.31^atons\;ha^{-1}$) and total digestible nutrients yield ($3.94{\pm}0.17^b$ vs. $4.59{\pm}0.21^atons\;ha^{-1}$). Dry matter percentage in corn stalks and corn ears was not different (P>0.05) between two treatments. It was concluded that corn-soybean intercropping strategy was promising technique in enhancing forage productivity though positive symbiotic relation between two crops.

• Journal of Bio-Environment Control
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• v.10 no.3
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• pp.155-158
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• 2001

The cultivation area of white spined cucumbers for export has continuously increased up to 121 ha in 2000. Since white spined cucumbers set fruits on lateral shoots, fruit yield is dependent upon the development of lateral shoots. Unfortunately, the major cultivation period is during winter season, October to February, when temperature and light are limited for the development lateral shoots. This experiment was conducted to investigate the effect of soil heating on the development of lateral shoots in white spined cucumbers. Number of lateral shoots were 13.7 and 11.7 in the soil heated treatment and in the control, respectively. Number of marketable fruits per plant was 45 in the soil heated treatment as compared to 38 in the control. Fruit yield was 81 ton per ha, an 18% increase, in the soil heated treatment. Soil heating not only increased number of long lateral shoots but also decreased curved fruits.

• Biomolecules & Therapeutics
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• v.27 no.6
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• pp.570-576
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• 2019

Particulate matter (PM), which refers to the mixture of particles present in the air, can have harmful effects. Damage to cells by PM, including disruption of organelles and proteins, can trigger autophagy, and the relationship between autophagy and PM has been well studied. However, the cellular regulators of PM-induced autophagy have not been well characterized, especially in keratinocytes. The Aryl Hydrocarbon Receptor (AhR) is expressed in the epidermis and is activated by PM. In this study, we investigated the role of the AhR in PM-induced autophagy in HaCaT cells. Our results showed that PM led to AhR activation in keratinocytes. Activation of the AhR-target gene CYP1A1 by PM was reduced by co-treatment with ${\alpha}$-naphthoflavone (${\alpha}-NF$), an AhR inhibitor. We also evaluated activation of the autophagy pathway in PM-treated keratinocytes. In HaCaT cells, treatment with PM treatment led to the induction of microtubules-associated proteins light chain 3 (LC3) and p62/SQSTM1, which are essential components of the autophagy pathway. To study the role of the AhR in mediating PM-induced autophagy, we treated cells with ${\alpha}-NF$ or used an siRNA against AhR. Expression of LC3-II induced by PM was decreased in a dose dependent manner by ${\alpha}-NF$. Furthermore, knockdown of AhR with siAhR diminished PM-induced expression of LC3-II and p62. Together, these results suggest that inhibition of the AhR decreases PM-induced autophagy. We confirmed these results using the autophagy-inhibitors BAF and 3-MA. Taken together, our results indicate that exposure to PM induces autophagy via the AhR in HaCaT keratinocytes.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.1
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• pp.57-61
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• 1994

The study was conducted at low lying areas in Pabna Sirajgong districts of Bangladesh. To observe the potentiality of bio-mass production two trials were conducted. In first trial maize intercropped with Khesari taking 15 experimental plots of each size $5m{\times}5m$ were arranged in 5 blocks having homogenous soil characteristics. The study showed that the bio-mass yield of sole maize and sole Khesari were 35.25 t/ha. and 56.80 t/ha. respectively and there was a significant difference (p < 0.01) among them. The results also showed that bio-mass yield of maize and Kherasi was higher ($70.04{\pm}6.25t/ha$, $98.88{\pm}10.77t/ha$ and $80.56{\pm}9.5t/ha$) compared to sole maize and sole Khesari and land equivalent ratio was also lower. For second trial, one hectare of land was divided into 16 experimental plots with 4 replications in each plot. Four levels of urea (0 kg/ha, 30 kg/ha, 45 kg/ha, and 60 kg/ha.) were applied to experimental plot. The seed rates were 98.8 kg/ha (farmer's practice), 86.45 kg/ha, 74.1 kg/ha and 61.75 kg/ha. average bio-mass yield of matikalai at different seed rates along with urea fertilizer ranged from 38.49 t/ha, to 65.35 t/ha. the highest seed rate along with highest fertilizer also correspond to the peak production (65.35 t/ha) and the lowest seed rate (61.75 kg/ha) along with the lowest fertilizer rate (30 kg/ha.) showed lowest production (38.49 t/ha.). Here, it was found that the bio-mass yield of matikalai increased with the incremental amount of seed, indicating significant effect (p < 0.05) of seed rates on the bio-mass yield of matikalai. On the other hand, fertilizer doses in different treatment combinations had significant effect (p < 0.05) on bio-mass yield. Two levels of seed rates at zero level of fertilizer were recommended : 86.45 kg/ha for the resource rich farmers and 61.75 kg/ha for the resource poor farmers.

• Korean Journal of Soil Science and Fertilizer
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• v.38 no.6
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• pp.321-327
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• 2005

Ammonia ($NH_3$) volatilization was measured from latex coated urea (LCU) and normal urea treated rice paddy under transplanting rice culture in Milyang in 2002 and 2003. The $NH_3$ volatilization from incubation experiment was significantly related with ammonium-N ($NH_4-N$) concentration and pH in the surface water. The correlation coefficients of $NH_3$ volatilization compared to the $NH_4-N$ and pH in surface water were significantly higher in urea than LCU. The $NH_3$ volatilization from both urea and LCU treatments was not increased in surface water of pH less than 8.0, while $NH_3$ volatilization increased significantly in the surface water of pH over 8.0. The results in the field experiment indicated that $NH_3$ volatilization after top-dressing of urea increased rapidly with increasing $NH_4-N$ concentration in soil and floodwater, and highest from 7 to 10 days after top-dressing. The amount of $NH_3$ volatilized from urea treatment was in the range of $4.9-8.4kg\;N\;ha^{-1}$. The variations of $NH_3$ volatilization in 2002 and 2003 were caused by changed N dynamics due to the different weather conditions such as rainfall and temperature. The amount of $NH_3$ volatilized from LCU treatment was significantly reduced compared to that of urea. The reason for the reduced $NH_3$ volatilization in LCU treatment would be due to the lower concentration of $NH_4-N$ in floodwater. The amount of $NH_3$ volatilized from LCU treated rice paddy was in the range of $1.2-1.8kg\;N\;ha^{-1}$, and the loss of N by ammonia volatilization was 2.0-2.3%. Loss of N by $NH_3$ volatilization with LCU treatment was reduced by 75-79% comparing to urea treatment.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.11
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• pp.5778-5784
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• 2013

In order to develop anti-wrinkle agent, we measured the anti-oxidative activity of gallic acid (GA) from Paeonia suffruticosa Andrews and investigated its cytotoxicity in HaCaT cells and then investigated its effect on tumor necrosis factor alpha (TNF-${\alpha}$)-induced matrix metalloproteinase-1 (MMP-1) mRNA, protein expressions and secretion in same cells. GA showed anti-oxidative activity with $IC_{50}$ of 30 ${\mu}g/mL$ and its activity was higher than that of butylated hydroxyanisol (BHA). GA showed weak cytotoxicity with high concentration (200 ${\mu}g/mL$) in HaCaT cells. MMP-1 mRNA, protein expression and secretion induced by tumor necrosis factor alpha (TNF-${\alpha}$) in HaCaT cells were significantly decreased by treatment of GA with dose-dependent manner(p<0.05). Therefore, our findings suggest that GA can be useful as an active ingredient for cosmeceuticals of anti-wrinkle effects.