• 한국입자에어로졸학회지
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• 제10권3호
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• pp.93-97
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• 2014

Condensation particle counter (CPC) has been one of the most important basic instrument for measuring number concentration of submicron aerosols. The principle of the CPC is to expose aerosols to a supersaturated vapor and cool down which causes adiabatic expansion. The particles grow by heterogenous nucleation to a sufficient size for easy detection by optical method. However, for growth by condensation, CPC essentially needs both saturater and condensor causing a heavy system. Therefore, it is hard to install commercial CPC to tethered balloon package system. In this study, we developed customized CPC for tethered balloon package system called Hy-CPC which is lighter and smaller in structure than commercial CPCs, and evaluated activation efficiency and detection efficiency by Hy-CPC using electrostatic method (electrometer and Faraday cup).

• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.978-984
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• 2004

In the course of screening for a novel inhibitor of CDC2, HY558-1 was isolated from a culture broth of Penicillium minioluteum F558. Moreover, it was found that HY558-1 had an effect on both the cell cycle regulation and apoptosis of human cervical adenocarcinoma HeLa cells. A flow cytometric analysis of HeLa cells revealed appreciable cell cycle arrest at the G1 and G2/M phases following treatment with HY558-1. Furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with HY558-1. To obtain further information on the cell cycle arrest and apoptotic induction induced by HY558-1, the expression of certain cell cycle and apoptosis-associated proteins was examined using a Western blot analysis. The results revealed that HY558-1 inhibited the phosphorylation of pRb and decreased the expression levels of CDK2, CDC2, and cyclin A in the cell cycle progression. It was also shown that the level of $p21^{WAF1/CIP1}$ was increased in HeLa cells treated with 0.52 mM of HY558-1. Accordingly, HY558-1 was found to inhibit the proliferation of HeLa cells through the induction of G1 phase arrest by inhibiting pRb phosphorylation via an upregulation of $p21^{WAF1/CIP1}$, and G2/M phase arrest by directly inhibiting CDC2 and cyclin A. Moreover, HeLa cells treated with 0.52 mM of HY558-1 exhibited apoptotic induction associated with the cleavage of Bid and release of cytochrome c from mitochondria into the cytosol. Subsequent investigation of the activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP) suggested that the mitochondrial pathway was primarily involved in the HY558-1-induced apoptosis in HeLa cells.

• 한국자원식물학회지
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• 제36권3호
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• pp.198-206
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• 2023

The effectiveness of the extracts of Alnus japonica and Portulaca oleracea, which are effective in improving alcohol-induced liver damage, was confirmed using acute and chronic alcoholic liver injury animal models. In the acute alcoholic liver injury model, dieting Alnus japonica and Portulaca oleracea complex (ALPOC) at a dose of 50 mg/kg showed no significant change in liver or body weight, while measured plasma ALT activity to be deficient (28.12 U/ml) compared to the alcohol intake group (42.5 U/ml), and confirmed that restored it to an average level. It showed an improvement of 34.9% compared to the alcohol intake group. AST activity confirmed that it showed a very effective liver protection activity by showing a gain of 12.6%. The chronic alcoholic liver damage animal model demonstrated that ALT showed an improvement effect of 25%, and AST showed an effect similar to that of the positive control group, Hovenia extract. In addition, through H&E staining analysis, observed that the ALPOC improved the necrosis and bleeding of the liver. And the ALPOC group showed intense antioxidant activity of 127% or more compared to the alcohol intake group, and this was confirmed to have a very high activity, which is more than 20% higher than that of the hovenia fruit extract.

• Journal of Microbiology and Biotechnology
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• 제12권3호
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• pp.470-475
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• 2002

In the course of screening for a novel cell cycle inhibitor, a potent Cdk 1 inhibitor, HY558, was found from the culture broth of Penicillium minioluteum F558 isolated from a soil sample. The molecular ion of HY558 was identified at m/z 329 (MH+) with a molecular formula of $C_20H_44ON_2$. HY558 exhibited selective antiproliferative effects on various human cancer cell lines. Its $IC_50$ values were estimated to be 0.29 mM on HepG2, 0.30 mM on HeLa, 0.30 mM on HL6O, 0.33 mM on HT-29, and 0.25 mM on AGS cells. Interestingly, Hy558 demonstrated no antiproliferative effect with normal lymphocytes used as the control, and a low level of inhibition on the proliferation of A549 cancer cells. A flow cytometric analysis of HepG2 cells revealed an appreciable arrest of cells at the G1 and G2/M phases of the cell cycle following treatment with Hy558. furthermore, DNA fragmentation due to apoptosis was observed in HeLa cells treated with 0.46 mM of HY558.

• Journal of Welding and Joining
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• 제14권3호
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• pp.48-57
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• 1996

A study was made to examine the characteristics of base metal and stress relief cracking(SRC) of heat affected zone(HAZ) for HY-100 and Cu-bearing HSLA-100 steels. The Gleeble thermal/mechanical simulator was used to simulate the SRC/HAZ. The details of mechanical properties of base plate and SRC tested specimens were studied by impact test, optical microscopy and scanning electron microscopy. The specimens were aged at $650^{\circ}C$ for HSLA-100 steel and at $660^{\circ}C$ for HY-100 steel and thermal cycled from $1350^{\circ}C$ to $25^{\circ}C$ with a cooling time of $\Delta$t_${800^{circ}C/500^{circ}C}$=21sec. corresponds to the heat input of 30kJ/cm. The thermal cycled specimens were stressed to a predetermined level of 248~600MPa and then reheated to the stress relief temperatures of $570~620^{\circ}C$. The time to failure$(t_f)$ at a given stress level was used as a measure of SRC susceptibility. The strength, elongation and impact toughness of base plate were greater in HSLA-100 steel than in HY-100 steel. The time to failure was decreased with increasing temperature and/or stress. HSLA-100 steel was more susceptible to stress relief cracking than HY-100 steel under same conditions. It is thought to be resulted from the precipitation of $\varepsilon$-Cu phase by dynamic self diffusion of solute atoms. By the precipitation of $\varepsilon$-Cu phase, the differential strengthening of grain interior relative to grain boundary may be greater in the Cu-bearing HSLA-100 steel than in HY-100 steel. Therefore, greater strain concentration at grain boundary of HSLA-100 steel results in the increased SRC susceptibility. The activation energies for SRC of HSLA-100 steel are 103.9kcal/mal for 387MPa and 87.6kcal/mol for 437MPa and that of HY-100 steel is 129.2kcal/mol for 437MPa.

• 한국미생물·생명공학회지
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• 제29권3호
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• pp.155-161
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• 2001

Lactrococcus sp. HY449균줄르 M17-glucose broth에 배양하여 배양 상등액으로부터 propanol-actone 침전 ion-exchange chromatography gel-filtration chromatography 및 reverse-phase chroamtography 등을 통하여 비활성 25,600,000 BU/mg 인 순수한 bacteriocin 을 정제하였다. 정제 과정 주에서 ion-exchange chromatography 단계에 서는 35.3%의회수율이 7.3%로 감소하였다. Reverse-Phase chromatography에선 3.3%의 회수율을 보였고 활성도는 413.5배로 증가하였다. Tricine-SDS 전기영도 결과 bacteriocin 은 단일 밴드로 나타났으며, N-말단 아미노산 서열 분석을 수행한 결과 $NH_2$-IIe-Leu-Pro-GIn로 확인되었다. 아미노산조정 분석결과를 바탕으로 분자량을 예측한 결과 본 bacteriocin은 32개의 아미노산으로 이루어져 있으며 분자량은 3.6kDa인 것으로 추정되었다.

• 사상체질의학회지
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• 제25권4호
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• pp.343-358
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• 2013

Objectives This study aimed to observe the effect of Hyangsayangwi-tang on the cisplatin-induced gastrointestinal dysfunctions in rats. Methods Four groups(each of 8 rats per group) were used in this study. Saline and distilled water treated control rats are Intact vehicle control group. Delayed gatrointestinal mortility was induced by intraperitoneal treatment of cisplatin 2mg/kg, once a week for 5 weeks(Cisplatin control group). Hyangsayangwi-tang aqueous extracts(HY) were orally administered in a volume of 5ml/kg, once a day for 14 days from 4th ciplatin treatmernt(HY group). Ondansetron 1mg/kg was subcutaneously treated, in a volume of 1ml/kg, as same as HY(ondansetron group). We measured the body weights, intestinal charcoal transit ratio, fecal parameters, fundus MDA, GSH contents and SOD, CAT activities, TPH and MAO activities, pyloric gastrin and serotonin contents with their immunoraective cells, colonic serotonin-immunoreactive cells, the histopathology of pylorus, fundus mucosa and colon. Results and Conclusions (1) The body weight gains, the small intestinal charcoal transfer rates, the fecal parameters(numbers, weights and water contents) were increased in HY, ondansetron group. (2) The inhibit of fundus antioxidant defense systems by cisplatin were decreased in HY, ondansetron group. (3) The pyloric TPH activities were increased and the pyloric MAO activities were decreased in HY group. (4) The pyloric gastric contents and the gastrin-immunoreactive cells were increased in HY group. And the pyloric serotonin contents and the pyloric and colonic serotonin-immunoreactive cells were decreased in HY group. (5) The pyloru atrophic changes and the gastric surface erosive damage regions by cisplatin were favorably inhibited by treatment of HY. HY, a representative Soeumin prescription improve GI dysfunctions and constipation retarded by cisplatin through modulations of GI enterochromaffin cells, serotonin and gastrin-producing cells and antioxidative systems. Especially HY showed the highest favorable effects more than those of ondansetron.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1591-1595
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• 2012

We previously isolated a novel compound, HY251, with the molecular structure of 3-propyl-2-vinyl-1,2,3,3a,3b,6,7,7a,8,8a-decahydrocyclopenta[a]indene-3,3a,7a,8a-tetraol from the roots of Aralia continentalis. The current study was designed to evaluate the detailed molecular mechanisms underlying the apoptotic induction by HY251 in human ovarian cancer PA-1 cells. TUNEL assay and Western blot analyses revealed an appreciable apoptotic induction in PA-1 cells treated with $60{\mu}M$ of HY251 for 24 h. This apoptotic induction was associated with caspase-8-dependent Bid cleavage, which in turn resulted in the formation of pro-apoptotic truncated Bid (tBid), and activation of caspase-9 and -3, as well as the cleavage of poly(ADP-ribose) polymerase (PARP). Moreover, we found that this death event was also associated with the significant up-regulation and activation of the p53 tumor-suppressor protein through phosphorylation at Ser15. Therefore, we suggest that HY251 may be a potent cancer chemotherapeutic candidate for the treatment of ovarian cancer.

• 농업과학연구
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• 제9권2호
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• pp.540-545
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• 1982

한국(韓國)에서 사육(飼育)되고 있는 닭의 육용종(肉用種) Hubbard bro.와 Cobb 및 난용종(卵用種) Hy line 그리고 우리나라에서 육종(育種)된 한협(韓協)325호(號)의 병아리를 공시동물(供試動物)로 하고 개방식중량측정형(開放式重量測定型) 호흡시험장치(呼吸試驗裝置)를 사용(使用)하여 에너지대사량(代謝量)을 측정(測定)한 결과(結果)는 다음과 같다. 1. 병아리를 9 주령(週齡)까지 cage에서 사육(飼育)한 1수당(首當) 평균(平均) 체중(體重)은 Hubbard bro.가 2570.7g이고 Hy line은 776.9g였으며 한협(韓協)325호(號)는 748g 이었다. 2. 주별(週別) 1 수당(首當) 사료섭취양모(飼料攝取量)은 3주령(週齡)에서 Hubbard bro. 가 54.6g으로 Hy line의 26.7g에 약(約) 2배(倍)였으며, 8령(週齡)에서는 151.1g과 57.2g 으로 약(約) 3배(倍) 정도(程度)였다. 음수량(飮水量)은 5주령(週齡)에서 Hubbard bro.가 1일(日) 평균(平均) $226m{\ell}$, Hy line 이 $58m{\ell}$, 8주령(週齡)에서는 $282m{\ell}$와 $70m{\ell}$였다. 3. 분(糞)의 배설량(排泄量)은 4주(週)와 8주령(週齡)에서 완전건조상태(完全乾燥狀態)로 1일(日) 1수당(首當) Hubbard bro.가 18.7g과 41.5g이었고 Hy line은 6.1g과 10.0g이었다. 4. 에너지 섭취량(攝取量)과 배설량(排泄量)은 3, 5, 8주령(週齡)에서 사료섭취(飼料攝取)에너지와 분(糞)의 배설(排泄)에너지를 측정(測定)하여 대사(代謝)에너지를 조사(調査)한 결과(結果) Hubbard bro. 는 75.4~77.1%였고 Hy line은 75.0~83.5%로 난용종(卵用種)의 사료이용율(飼料利用率)이 높았다. 5. 병아리의 호흡상(呼吸商)은 육용종(肉用種)이나 난용종간(卵用種間)에 차이(差異)를 인정(認定)할 수 없었으며 0.78에서 0.97사이였는데 사료섭취량(飼料攝取量)이 적으며 0.78로 떨어졌으며 많아짐에 따라 0.97로 증가(增加)하였다. 6. 대사체중(代謝體重)에 대한 에너지의 발생량(發生量)은 83.1~123.1Kcal로서 24시간(時間)동안 사료(飼料)의 섭취(攝取)를 거의 하지 않은 상태(狀態)에서는 83.4Kcal로 기초대사량(基礎代謝量)에 근사(近似)하였고 동물상내(動物箱內)의 온도(溫度)가 $27{\sim}34^{\circ}C$로 높을 때에는 123.1Kcal로 증가(增加)하는 현상(現像)을 나타냈다.

• 한국항공우주학회지
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• 제43권3호
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• pp.243-256
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• 2015

극초음속 비행체 개발 연구의 일환으로 소형 발사체나 과학로켓을 이용한 여러 비행 시험 프로그램이 진행되었다. 국제 협력으로 진행된 호주의 HyShot 프로그램은 과학로켓을 이용한 대표적인 스크램제트 엔진 비행시험 프로그램이며, 이후 다양한 탑재체에 대한 유사한 극초음속 비행 시험 프로그램들이 진행되었다. 미국에서는 DARPA와 AFRL 및 ONR이 각각 Falcon HTV-2, X-51A 및 HyFly의 프로그램을 수행하였으며, 호주와 국제협력으로 HyCAUSE, HIFiRE 비행시험 프로그램을 수행하였다. 그 밖에도 프랑스는 X-51A과 유사한 LEA 프로그램을 진행하고 있으며, 러시아 및 중국, 인도는 극초음속 방위 체계 개발을 목표로 비행시험을 진행하고 있는 것으로 보인다. 본 논문에서는 향후 국내에서 유사 프로그램을 수행할 시 참고할 수 있도록, 이들 프로그램의 목표 및 기술 요소와 경과 및 프로그램 사이의 관계 등을 정리하였다.