• 미생물학회지
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• 제30권2호
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• pp.88-95
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• 1992

유전공학 기법으로 번형시킨 Km' plasmid 들의 전이를 30.deg.C 의 Luris-Bertani broth 에서 실시하였으며, 그 전이 빈도에 대한 donor 와 recipient 의 균주와 함께 pH 의 영향을 연구하였다. GEM 균주들과 NI 균주의 Km'plasmid 의 전이빈도는 recipient 가 MTI 일 경우 pH 7 에서 약 $10^{-5}$ 로 비슷하였으나 CS 균주인 DKC601 에서는 Km'plasmid 의 전이빈도가 $2.2 * 10^{-7}$ 로 훨씬 떨어졌다. 그리고 recipient 가 lab. strain (E. coli HB101) 인 경우에도 Km'plasmid 의 전이경향은 GEM 균주들과 NI 균주사이에 큰차이가 없었다. 또 어느 균주의 경우에나 pH 7 일 때의 전이 빈도가 $10^{-5}$ 정도로 가장 높게 나타났으며, pH 5 와 pH 9 에서는 그보다 조금 떨어졌다. 그러나 E. coli C600을 host 로 하여 제조한 CS 균주(DKC601)에서는 Km'plasmid 의 전이 빈도가 다른 GEM 균주들에 비하여 $10^{2}$ $-10^{3}$ 배 낮았으며 pH 4와 9 에서 6 시간의 conjugation 후에도 전이가 전혀 일어나지 않았다. Conjugant 에서의 plasmid의 재배열은 lab, strain 을 recipient 로 했을 경우에는 별로 없었지만, NI 균주를 recipient 로 했을 경우에 많이 나타났으며, 특히 donor 가 NI 균주보다 GEM 균주인 경우에 더욱 다양하였다. 그러나 pH 에 따른 타이는 그렇게 크지 않았다.

• 대한세포병리학회지
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• 제15권2호
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• pp.92-100
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• 2004

Due to insufficient clinical information, most cervicovaginal smears from pregnant or postpartum women have been screened without regard to pregnancy-related cytological changes. Here, we have reviewed 116 abnormal cervicovaginal smears from 103 pregnant and postpartum women. Initial cytological diagnoses revealed the following: 9 cases of high-grade squamous intraepithelial lesions (HSIL), 8 cases of low-grade squamous intraepithelial lesions (LSIL), 85 cases of atypical squamous cells of undetermined significance (ASCUS), and 14 cases involving atypical glandular cells of undetermined significance (AGUS). 31 cases upon review, involved pregnancy-related cytological changes, comprising 25 cases of decidua cells, 4 cases of Arias-Stella reaction, and 2 cases of cedidual cells coupled with Arias-Stella reaction. Interpretation errors were detected in 14 cases: 13 cases of decidual cells interpreted as either ASCUS favor reactive or ASCUS ruled out HSIL, and one case of Arias-Stella reaction was interpreted as ASCUS ruled out HSIL. Decidual cells and degenerated glandular cells with Arias-Stella reaction can result in diagnostic mistakes. In order to avoid misdiagnosis and unnecessary surgeries, both clinicians and pathologists must be aware of the pregnancy-related cytological changes. The clinician should also always inform the pathologist on the pregnancy status of the patient.

• 대한세포병리학회지
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• 제14권2호
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• pp.60-65
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• 2003

Objective: The sensitivity of the AutoPap Primary Screening System with Location-Guided Screening (AutoPap LGS) for Identifying atypical cells in cervicovaginal smears was evaluated. Methods: Two hundred forty one slides with atypical cervical cytology randomly sampled were rescreened both manually and by the AutoPap LGS. The AutoPap LGS localized the atypical cells as 15 fields of view(FOVs), which were reexamined by manual review. The sensitivity was also evaluated in accordance with the cellularity of the smears. Results: The AutoPap LGS successfully processed 232 out of 241 slides. The sensitivity of the AutoPap LGS identifying the atypical cells in successfully processed slides was 97.4%(226/232). The false negative rate was 2.6%(6/232). There was no false negative case on high grade squamous intraepithelial lesion (HSIL) or squamous cell carcinoma(SCC) smears in the AutoPap LGS. The FOVs localized the diagnostic-atypical cells in 97.8%(221/226). The number of diagnostic-atypical FOVs was increased in higher-degree of atypical cytology. The AutoPap LGS localized the atypical cells in 100% of adequately cellular smears and in 92.5% even in low cellular smears. Conclusion: The AutoPap LGS showed relatively good sensitivity to detect atypical cells. It can be a valuable system to localize atypical cells, especially in HSIL or cancer slides, even in smears with low cellularity.

• 한국환경복원기술학회지
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• 제13권1호
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• pp.57-68
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• 2010

The purpose of this study is to analyze the characteristics of environment-friendliness of waterproof materials based on comprehensive experiments on waterproofness in terms of coefficients of permeability, harmfulness of waterproof materials and fish toxicity of Oryzias latipes mortality to verify eco-toxicity of each method of construction and waterproof material, which are to be applied by taking eco-toxicity into account when building ecological flows in upper areas on natural and artificial grounds. As a result, the following conclusions have been reached in this study: 1. In regard of the harmfulness analyzed, each material showed a different result of analytical value in each lab tank. Compared to input water, pH, COD, SS, T-P, and T-N values increased a little, but DO value decreased. The value of turbidity analyzed independent of the water quality standard of aquatic ecosystem set forth by the Ministry of Environment increased a little compared to the value in input water. 2. In the experiment of fish toxicity, compacted quicklime, cement fluid waterproof material, cement mortar waterproof material and bentonite powder were found to have 100% of fish mortality, respectively, and membrane waterproof material showed 83.3% of mortality, indicating strong fish toxicity. Improved asphalt sheet (63.3%) and synthetic rubber sheet (53.3%) were analyzed to have medium fish toxicity, while bentonite sheet (6.7%), Hwang-toh (6.7%) and clay (3.3%) showed relatively lower mortality and fish toxicity. 3. Regarding the analysis on waterproofness in terms of the coefficient of permeability of each waterproof material, improved asphalt sheet, synthetic rubber sheet, membrane waterproof material, cement fluid and mortar waterproof material and bentonite sheet were found impervious in case no leakage takes place in construction. Bentonite powder was found practically impervious based on the analytical results from the experiment done in compliance with weight ratios. So were the clay and Hwang-toh from the experimental results. To sum up such results as found in the experiment mentioned so far, the values of harmfulness and waterproofness analyzed were different in each lab tank, but there was absolutely little correlation with the mortality gained from the experiment on fish toxicity. In the experiment of fish toxicity, environment-friendly waterproof materials were analyzed, and it was found that clay, Hwang-toh and bentonite sheet are highly environment-friendly. In contrast, synthetic rubber and improved asphalt sheets were found to have medium-level environment-friendliness. Also, membrane water-proof materials, compacted quicklime, cement fluid and mortar waterproof material and bentonite powder were analyzed to have low environment-friendliness.

• Asian-Australasian Journal of Animal Sciences
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• 제23권8호
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• pp.1028-1033
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• 2010

The effects of delta-aminolevulinic acid (DALA) or chito-oligosaccharide (COS) in layer diets on production performance, egg quality and hematological characteristics were investigated in this 8-week trial. Two hundred and seventy 27-week-old (Hy-Line brown) layers were randomly assigned to 5 treatments with 9 replications per treatment. Dietary treatments were i) Control (basal diet); ii) DALA1 (basal diet+DALA 2 mg/kg); iii) DALA2 (basal diet+DALA 4 mg/kg); iv) COS1 (basal diet+COS 100 mg/kg) and v) COS2 (basal diet+COS 200 mg/kg). In this study, no significant difference was observed in feed intake and egg shell quality (eggshell breaking strength and egg shell thickness) among treatments. The inclusion of DALA increased egg production during the first 4 weeks. Egg weight was increased in both DALA (quadratic, p<0.05) and COS (linear, p<0.05) treatments compared with the control treatment. Laying hens fed the DALA treatment had an increased yolk color unit (quadratic, p<0.05) and haugh unit (linear, p<0.05) compared with the control group. Similarly, these characteristics were also affected by COS treatments, with both values being linearly increased (p<0.05) in COS treatments compared with the control treatment. Additionally, birds fed DALA treatments significantly increased (quadratic, p<0.05) the number of RBC, WBC and lymphocytes compared with the control treatment. Dietary DALA supplementation linearly increased (p<0.05) the serum iron concentration at the end of the 8th week. The inclusion of COS increased (linear, p<0.05) the concentration of RBC, WBC and lymphocytes compared with the control treatment. In conclusion, dietary DALA at the lower dosage (2 mg/kg) could exert better effects in laying hens than higher dosage (4 mg/kg). Birds fed DALA supplemented diet had an increased iron availability, egg weight, eggshell quality and immunity. Moreover, the inclusion of COS (200 mg/kg) can increase egg weight, eggshell quality and immunity in laying hens. Therefore, both the utilization of COS and DALA could be considered as a new strategy for optimizing egg quality and health condition of laying hens.

• 농약과학회지
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• 제7권1호
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• pp.58-65
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• 2003

농업용 약물로서의 활용성 진단과 예측을 위한 기초 자료로서 상용화 된 제초제와 같은 분자량을 가지는 의약 각 245 종에 대한 10 가지의 물리 -화학 파라미터들을 계산하고 제초제와 광합성 (PS-II) 저해제 및 acetolactase synthase (ALS) 저해제들의 특정한 물리-화학 파라미터들에 대한 수치 범위를 비교 검토하였다. 제초제들의 특정 물리-화학 파라미터에 대한 85% 의존적 수치 범위는 소수성 상수 (Obs. logP): $-0.90\sim4.50$, 쌍극자 능율 (DM): $1.80\sim12.22$ Debye, van der Waals 분자부피 (Vol.); $558\sim995Cm^3$ 및 표면적 (S.Area): $194\sim356\;{\AA}^2$, molar refractivity (MR): $53\sim104Cm^3/mol$., 분극율 (Pol): $19\sim37\;{\AA}^3$, 분자량 : $202\sim430(amu)$, 및 수화 에너지 (Hy.E): $-10.16\sim114.7$ Kcal/mol 등 이었다. 그리고 작용 기작에 따라 물리-화학파라미터의 범위값을 특징적으로 나타내고 있음을 알았으며 MR 상수와 분극율은 의약과 제초제 (ALS 저해제)를 구분하는 판별 가능한 요소가 될 것으로 예상되었다.

• 농업과학연구
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• 제37권1호
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• pp.53-60
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• 2010

Two experiments were conducted to investigate the effect of probiotics on the malodor removal. In experiment 1, dietary effects (several malodorous gas concentration of excreta, dry matter metabolizability, and blood profiles) were determined using laying hens. A total of 30 Hy-line brown layers, 68-wk of age, were randomly allocated into 5 groups with 3 replicates of 2 birds each. The treatments were probiotics free, 0.2% and 0.4 % addition of mixed probiotics (Bacillus subtilis PNG-4 +Lactobacillus acidophilus LAS), and 0.2 and 0.4 % addition of single probiotics (Bacillus subtilis PNG-4). In experiment 2, the effects of mixing of probiotics into the excreta on the malodorous gas removal was investigated. There were three treatments (probiotics free, Bacillus subtilis PNG-4 +Lactobacillus acidophilus LAS, and Bacillus subtilis PNG-4) with three replicates. The malodorous gas concentrations were detected at 0, 3, 7 and 14 day of incubation. In experiment 1, ammonia concentration was significantly decreased by feeding mixed probiotics at 14th day of incubation. However, amines, hydrogen sulfide, ethylmercapthan, and methylmercapthan were not significantly affected by mixed probiotics. Dry matter metabolizability was significantly increased by feeding probiotics, but no significant differences between single and mixed probiotics. There was no significant differences in blood profiles. In experiment 2, mixing of probiotics into the excreta did not affect the concentration of ammonia, amines, hydrogen sulfide, ethylmercapthan, and methylmercapthan. Therefore, these experiments suggested that Bacillus subtilis PNG-4 + Lactobacillus acidophilus LAS supplementations could improve ammonia gas removal, and dry matter metabolizability in layers. Also, decrease of ammonia concentration was higher in mixed probiotics group compare to the single probiotics group. On the other hand, mixing of probiotics into the excreta appeared not to be a useful method.

• 한국가금학회지
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• 제33권2호
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• pp.127-131
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• 2006

본 시험은 expanding 가공 기술이 산란계의 생산성, 계란 품질과 영양소 소화율에 대하여 알아보고자 실시하였다. 49주령 Hy-line Brown 270수를 공시하였고, 6주간 시험을 실시하였다. 1) MS(mash), 2) EC(expanded crumble) 및 3) EP( expanded pellet) 으로 3처리구로 구성하였으며, 처리 당 15반복, 반복당 6수씩 완전임의 배치하였다. 산란율은 EC와 EP 처리구가 MS 처리구와 비교하여 유의적으로 높게 나타났다(p<0.05). 그러나 난중은 처리구간에 유의적인 차이는 없었다(p>0.05). 난각 강도는 EC와 EP 처리구가 MS 처리구와 비교하여 유의적으로 높게 나타내었지만(p<0.05), 난각 두께는 처리 구간에 유의적인 차이를 나타내지 않았다(p>0.05). 난황색은 EC 처리구가 MS 처리구와 비교하여 유의적으로 높게 나타났다(p<0.05). 난황 계수는 EC와 EP 처리구가 MS 처리구와 비교하여 유의적으로 높게 나타났다(p<0.05). Haugh uint에 있어서 처리구간의 유의적인 차이는 없었으나(p>0.05), 변화량에 있어서 EP 처리구가 가장 높게 나타났다(p<0.05). 영양소 소화율에 있어서 건물, 칼슘, 인 소화율에 있어서 EP 처리구가 소화율을 개선시켰다(p<0.05). 본 연구 결과는 2차적인 사료 가공 기술이 산란계에 서 계란 품질과 영양소 소화율을 개선하였다.

• 한국가금학회지
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• 제32권2호
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• pp.143-147
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• 2005

팽이버섯 배지 부산물(F. velutipes media by-product)의 사료 자원화를 위하여 산란계 사료에 0, 5 및 $10\%$를 12주간 하이라인 갈색산란계 360수에게 급여하였다. 전 시험 기간 동안의 산란율, 난중, 산란량, 사료요구율 및 생존율은 대조구와 차이를 보이지 않았다. 사료섭취량은 대조구에서 120.5g 으로 T2의 123.9g보다 유의적으로 적었다(p<0.05). 난각강도, 난각두께 및 호우유니트도 시험 전 기간 동안 처리구간에 차이를 보이지 않았다. 그러나 난황색은 대조구, T1, T2에서 각각 7.92, 7.29 및 6.83으로 대조구에 비하여 T2에서 유의적으로 낮았다(p<0.05). 결론적으로 팽이버섯배지부산물을 산란계 사료원료로서 $5\%$를 급여하여 대조구와 비교시 생산성 및 계란의 품질에 미치는 영향이 없었으므로 사료자원으로 이용이 가능하지만, $10\%$를 급여시에 난황색이 현저하게 저하되었으므로 착색제의 첨가가 필요하다고 사료된다.

• 한국축산시설환경학회지
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• 제14권1호
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• pp.47-52
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• 2008

1. 조사농장의 산란율은 하이라인, 이사브라운 모두 $85{\sim}93%$ 수준 이였으며, 농가자체 파란율은 하이라인 $1.5{\sim}2.2%$, 이사브라운 $1{\sim}1.4%$이었다. 2. 계란 이송단계별 파란발생비율은 집란벨트 4.7%> 엘리베이터 2.5%> 선란기 1.7%> 컨베이어 0.9% 순 이었다. 3. 집란벨트에서 엘리베이터로 이송 중 계란이송가이드를 설치할 경우 약 1%의 파란경감 효과가 있는 것으로 나타났다. 4. 하이라인 브라운은 주령이 높아지면서 파란율은 높아지고, 파각력은 낮아지는 것으로 나타났으며, 회귀계수 분석 유의성은 $R^2=0.7$로 파각력의 변화가 파란율에 영향을 미치는 것으로 분석되었다. 5. 이사브라운에서는 환우 전에 비하여 환우 후 파각력이 높아진 것으로 나타났다. 6. 난각 두께와 파각력, 그리고 접촉 후 파각변위와 파각력과의 관계에서 이들 함수의 변수간에 회귀관계는 유의성이 나타나지 않았다.