• Journal of Microbiology
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• 제43권2호
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• pp.179-182
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• 2005

The inefficiency of in vivo gene transfer using currently available vectors reflects a major hurdle in cancer gene therapy. Both viral and non-viral approaches that improve gene transfer efficiency have been described, but suffer from a number of limitations. Herein, a fiber-modified adenovirus, carrying the small peptide ligand on the capsid, was tested for the delivery of a transgene to cancer cells. The fiber-modified adenovirus was able to mediate the entry and expression of a $\beta$-galactosidase into cancer cells with increased efficiency compared to the unmodified adenovirus. Particularly, the gene transfer efficiency was improved up to 5 times in OVCAR3 cells, an ovarian cancer cell line. Such transduction systems hold promise for delivering genes to transferrin receptor overexpressing cancer cells, and could be used for future cancer gene therapy.

• Journal of Construction Engineering and Project Management
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• 제3권2호
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• pp.35-48
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• 2013

Weak competencies in urban local bodies (ULBs) for implementing public private partnership (PPP) projects has been recognized as a major hurdle in uptake of PPP model in India. In this context, a questionnaire survey was conducted to evaluate perception of urban PPP experts and ULB representatives on three aspects related to competency development, including, critical competencies for implementing urban PPP projects, approaches for competency development in ULBs and constraints faced by ULBs in development of competencies. The five critical competencies identified are project identification, project conceptualization, transaction design, PPP process management and contract management. The approach of training programs for municipal staff and elected representatives, and capacity building oriented technical assistance for implementing urban PPP projects have been preferred by survey respondents for competency development. The frequent transfer of municipal decision makers and low motivation among municipal staff have been identified as the most significant constraints for development of competencies.

• Biomolecules & Therapeutics
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• 제23권5호
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• pp.391-399
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• 2015

As a major component of the epidermal tissue, a primary keratinocyte has served as an essential tool not only for the study of pathogenesis of skin-related diseases but also for the assessment of potential toxicities of various chemicals used in cosmetics. However, its short lifespan in ex vivo setting has been a great hurdle for many practical applications. Therefore, a number of immortalization attempts have been made with success to overcome this limitation. In order to understand the immortalization process of a primary keratinocyte, several key biological phenomena governing its lifespan will be reviewed first. Then, various immortalization methods for the establishment of stable keratinocyte cell lines will be explained. Finally, its application to a three-dimensional skin culture system will be described.

• 월간양계
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• 제33권1호통권375호
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• pp.120-123
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• 2001

닭고기는 도계공정과 취급과정에서 미생물이 오염되고 지방산화가 촉진되어 쉽게 변질되기 때문에 타 식육에 비해 저장성이 문제되는 육류식품이다. 저장성에 가장 영향을 미치는 것은 도계 공정중에 일어나는 상호오염 문제이다. 왜냐하면 닭 표피에 오염된 미생물은 나중에 수세나 염소수(chlorine)로 처리하여도 쉽게 제거되지 않기 때문이다. 따라서 위생적인 도계생산이 저장을 위한 필수 선결요소이다. 그리고 도계후 처리과정에서 작업자의 위생적인 인식이 요구되며, 가능한 단시간내에 포장을 하여 냉장 및 냉동 유통을 실시하여야 한다. 도계 및 수세공정중에 기존 염소수외에 초음파나 오존을 사용하거나 이산화염소나 인산염, pH 조정제 등을 첨가하면 미생물 억제효과가 크다고 보고되고 있다. 포장한 닭고기에 조사를 실시하면 2배 이상 저장기간을 연장시킬 수 있어 우리나라에서도 법적 허용 가능성을 검토 할 필요가 있다. 이와 같이 닭고기에 단일방법보다 hurdle technology를 이용한 복합적인 방법을 활용해야 저장기간을 연장할 수 있다.

• 가족자원경영과 정책
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• 제6권1호
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• pp.73-85
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• 2002

This study examined which factors influence the parental borrowing for children's college education in the U.S. A double-hurdle model was applied to analyze both the parents’decision to borrow and the amount of borrowing. Parents’income and total costs of college attendance had significant positive effects on both the decision to borrow and the amount borrowed. Students’income, parents’cash and savings had significant negative impacts on the amount borrowed, while home equity and the students’expected future income were significant positive factors. Greater amounts of grants significantly increased the probability of borrowing, but reduced the loan amount. Parents with smaller household size, and those who were college graduates were more likely to borrow. White parents borrowed greater amounts than did their non-White counterparts. The age of the student was a significant negative factor in the probability, as well as the level, of borrowing.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 추계학술대회 논문집 전기물성,응용부문
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• pp.158-159
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• 2006

The resistance-capacitance (RC) delay of signals through interconnection materials becomes a big hurdle for high speed operation of semiconductors which contain multilayer interconnection layers. In order to reduce the RC delay, low-k materials will be used for inter-metal dielectric (IMD) materials. We have developed self-consistent simulation tool that includes neutral-species transport model, based on the relaxation continuum (RCT) model. We present the parametric study of the modeling results of a two-frequency capacitively coupled plasma (2f-CCP) with $N_2/H_2$ gas mixture that is known as promising one for organic low-k materials etching. We include the neutral transport model as well as plasma one in the calculation. The plasma and neutrals are calculated self-consistently by iterating the simulation of both species till a spatiotemporal steady state profile could be obtained.

• Architectural research
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• 제22권4호
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• pp.145-154
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• 2020

Zoning has long been criticized for its negative effects and has been taken for granted that zoning works as a hurdle to urban diversity. Responses in the US have emphasized more fine-grained zoning approaches that plan mixed use. This paper introduces and evaluates Seoul's zoning system as a possible alternative. While US zoning regulations have relied on distinguishing ever larger numbers of land use zoning categories, Seoul has opted to integrate new land uses into existing classifications, allowing for greater flexibility of use in each zone. Using municipal building records to evaluate land use in three mid-density residential districts, this paper demonstrates that Seoul's flexible zoning is capable of producing diverse mixed use neighborhoods. It then highlights the potential downside of this approach, showing that flexibility allows for the commercialization and sectoral gentrification of residential districts. It concludes by suggesting that a combination of flexible zones and more fine-grained plans would capture the advantages of both US and Korean planning.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.133.2-134
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• 2003

The major hurdle of conventional chemotherapeutics is the toxicity to normal tissue. The possible therapeutic advantage(s) of nano-particle encapsulated chemotherapeutics (nano-molecules) may be the enhanced permeability and retention (EPR) effect. Nano-molecules with increase volume may incorporated into the tumor tissue selectively, which is composed of rather sparse structure. EPR effect may cause of increased effectiveness with lower tixicity to normal tissue of nano-chemotherapeutics. (omitted)

• BMB Reports
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• 제54권5호
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• pp.246-252
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• 2021

The Golgi complex plays a central role in protein secretion by regulating cargo sorting and trafficking. As these processes are of functional importance to cell polarity, motility, growth, and division, there is considerable interest in achieving a comprehensive understanding of Golgi complex biology. However, the unique stack structure of this organelle has been a major hurdle to our understanding of how proteins are secreted through the Golgi apparatus. Herein, we summarize available relevant research to gain an understanding of protein secretion via the Golgi complex. This includes the molecular mechanisms of intra-Golgi trafficking and cargo export in the trans-Golgi network. Moreover, we review recent insights on signaling pathways regulated by the Golgi complex and their physiological significance.

• International Journal of Computer Science & Network Security
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• 제21권1호
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• pp.34-39
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• 2021

Collaborative ontology construction is the latest trend in developing ontologies. In this technique domain specialists and ontologists need to work together. Because of the complexity associated with ontology construction, it's done in an iterative and incremental fashion. After each iteration, an ontology increment will be produced. Current ontology increment is always an enhanced version of the previous increment. Each ontology increment has to be verified for its accuracy. Domain specialists' contribution is very significant in accomplishing this necessity. Unfortunately, non-computing domain specialists (i.e. medical doctors, bankers, lawyers) are illiterate on semantic concepts. Therefore, validating the accuracy of the ontology increment is a complex hurdle for them. This research proposes verbalization approach to address this complexity.