• Animal cells and systems
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• v.16 no.1
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• pp.11-19
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• 2012

A major concern regarding the collection and storage of biodiversity information is the inefficiency of conventional taxonomic approaches in dealing with a large number of species. This inefficiency has increased the demand for automated, rapid, and reliable molecular identification systems and large-scale biological databases. DNA-based taxonomic approaches are now arguably a necessity in biodiversity studies. In particular, DNA barcoding using short DNA sequences provides an effective molecular tool for species identification. We constructed a large-scale database system that holds a collection of 5531 barcode sequences from 2429 Korean species. The Korea Barcode of Life database (KBOL, http://koreabarcode.org) is a web-based database system that is used for compiling a high volume of DNA barcode data and identifying unknown biological specimens. With the KBOL system, users can not only link DNA barcodes and biological information but can also undertake conservation activities, including environmental management, monitoring, and detecting significant organisms.

• Molecules and Cells
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• v.24 no.2
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• pp.200-209
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• 2007

We generated gene expression data from the tissues of 50 gastric cancer patients, and applied meta-analysis and gene set analysis to this data and three other stomach cancer gene expression data sets to define the gene expression changes in gastric tumors. By meta-analysis we identified genes consistently changed in gastric carcinomas, while gene set analysis revealed consistently changed biological themes. Genes and gene sets involved in digestion, fatty acid metabolism, and ion transport were consistently down-regulated in gastric carcinomas, while those involved in cellular proliferation, cell cycle, and DNA replication were consistently up-regulated. We also found significant differences between the genes and gene sets expressed in diffuse and intestinal type gastric carcinoma. By gene set analysis of cytogenetic bands, we identified many chromosomal regions with possible gross chromosomal changes (amplifications or deletions). Similar analysis of transcription factor binding sites (TFBSs), revealed transcription factors that may have caused the observed gene expression changes in gastric carcinomas, and we confirmed the overexpression of one of these, E2F1, in many gastric carcinomas by tissue array and immunohistochemistry. We have incorporated the results of our meta- and gene set analyses into a web accessible database (http://human-genome.kribb.re.kr/stomach/).

• Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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• v.8
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• pp.247-272
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• 2003

Mit der Entwicklung der Informations- und Kommunikationstechnologien $er\"{o}ffnen$ $Internet-unterst\"{u}tzte$ Lehren und Lernen neue $M\"{o}glichkeiten{\;}f\"{u}r$ den Sprach- und Kulturunterricht. Das Internet bietet den $Sch\"{u}lern$ vor allem $gro{\ss}es$ Interesse und $gro{\ss}e$ Motivation $f\"{u}r$ die deutsche Sprache und Kultur. In der vorliegenden Arbeit geht es vor allem um die praktische Anwendung von den $M\"{a}rchentexten$ in der germanistischen Abteilung der koreanischen $Universit\"{a}ten$. $M\"{a}rchen$ sind ein Kulturgut, das sich in vielen $V\"{o}ilkern$, Kulturen und Gegenden der Erde findet. $M\"{a}rchen$ sprechen eine Sprache der Symbole und Bilder, eine Sprache, die den Menschen in seiner $Emotionalit\"{a}t$ besonders anspricht. Sie haben eine Anziehungskraft, die uns nicht $unber\"{u}hrt{\;}l\"{a}sst$. Sie zeigen in vie1en Kulturen Motive menschlichen Daseins, die $\"{u}bereinstimmende$ Merkmale zeigen. In dieser Studie wird insbesondere $\"{u}ber$ eine untenichtspraktische Anwendung des $M\"{a}rchens$ $f\"{u}r$ den Deutschunterricht diskutiert. In dieser Arbeit bezieht sich der wissenschaftliche Gebrauch des Kulturbegriffes meist auf kollektive Erscheinungen, die Gruppen von Individuen einigen. Mit Hilfe des Internets kann man verschiedene Kulturelemente in der bestimmten deutschen Stadt finden. Zum Beispiel hat die freie Hansestadt Bremen die wohlgeformte Homepage. In dieser Homepage(http://www.bremen.de/) gibt es viele Informationen $\"{u}ber$ die Stadt Bremen; Geschichte, Wrrtschaft, Wissenschaft, Schulsystem, Kultur, Sport, Freizeit usw. Schuler lernen erst elementare $W\"{o}rter$, Grammatik und dann wichtige kulturelle Elemente. $Erw\"{u}nscht$ waren wissenschaftlich fundierte Methoden $f\"{u}r$ den deutschen Kulturuntenicht und systematisch aufgebaute Internetseiten, die fur bestimmte Deutschkurse an den koreanischen $Universit\"{a}ten$ gedacht sind. Es ware ideal, audiovisuelle/multimediale Webseiten/Webpages mit Texten, Bildern, Webaufgaben und Audio-Nideofiles zu erstellen.

• Proceedings of the Korean Society of Crop Science Conference
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• 2004.04a
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• pp.12-27
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• 2004

Thanks to spectacular advances in the techniques for identifying proteins separated by two-dimensional electrophoresis and in methods for large-scale analysis of proteome variations, proteomics is becoming an essential methodology in various fields of plant sciences. Plant proteomics would be most useful when combined with other functional genomics tools and approaches. A combination of microarray and proteomics analysis will indicate whether gene regulation is controlled at the level of transcription or translation and protein accumulation. In this review, we described the catalogues of the rice proteome which were constructed in our program, and functional characterization of some of these proteins was discussed. Mass-spectrometry is a most prevalent technique to identify rapidly a large of proteins in proteome analysis. However, the conventional Western blotting/sequencing technique us still used in many laboratories. As a first step to efficiently construct protein data-file in proteome analysis of major cereals, we have analyzed the N-terminal sequences of 100 rice embryo proteins and 70 wheat spike proteins separated by two-dimensional electrophoresis. Edman degradation revealed the N-terminal peptide sequences of only 31 rice proteins and 47 wheat proteins, suggesting that the rest of separated protein spots are N-terminally blocked. To efficiently determine the internal sequence of blocked proteins, we have developed a modified Cleveland peptide mapping method. Using this above method, the internal sequences of all blocked rice proteins (i. e., 69 proteins) were determined. Among these 100 rice proteins, thirty were proteins for which homologous sequence in the rice genome database could be identified. However, the rest of the proteins lacked homologous proteins. This appears to be consistent with the fact that about 30% of total rice cDNA have been deposited in the database. Also, the major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that fumed out to be calreticulin, gibberellin-binding protein, which is ribulose-1,5-bisphosphate carboxylase/oxygenase activate in rice, and leginsulin-binding protein in soybean have functions in the signal transduction pathway. Proteomics is well suited not only to determine interaction between pairs of proteins, but also to identify multisubunit complexes. Currently, a protein-protein interaction database for plant proteins (http://genome .c .kanazawa-u.ac.jp/Y2H)could be a very useful tool for the plant research community. Recently, we are separated proteins from grain filling and seed maturation in rice to perform ESI-Q-TOF/MS and MALDI-TOF/MS. This experiment shows a possibility to easily and rapidly identify a number of 2-DE separated proteins of rice by ESI-Q-TOF/MS and MALDI-TOF/MS. Therefore, the Information thus obtained from the plant proteome would be helpful in predicting the function of the unknown proteins and would be useful in the plant molecular breeding. Also, information from our study could provide a venue to plant breeder and molecular biologist to design their research strategies precisely.

• International Area Studies Review
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• v.20 no.2
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• pp.141-172
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• 2016

The aim of this study was to view Korean Hallyu stars, the advertisement models of Korean cosmetics brands, as human brands in the Chinese cosmetics market. Furthermore, this study attempted to verify whether the attributes of human brands, such as reliability, professionalism, attraction, intimacy, similarity and favorable impression, affect Chinese consumers' affection toward Korean celebrities, and whether such Chinese consumers recognize the authenticity of the products, sellers, and companies of Korean cosmetics brands advertised by Korean stars. The results of empirical analysis indicated that among the attributes of Korean stars, the advertisement models of Korean cosmetics companies, Chinese consumers' affection toward Korean stars was positively affected by reliability, attraction and similarity, but not by professionalism, intimacy and favorable impression. Furthermore, results showed that the consumers' affection toward Korean stars positively affected the authenticity of the brands advertised by the celebrities.

• 한국노년학
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• v.38 no.1
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• pp.27-45
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• 2018

The purpose of this study was to identify between the successful aging-related predictive factors and the successful aging in the middle aged office. The predictive factors of successful aging such as subjective health condition, cognitive functions, self-esteem, and social support were confirmed by the existing literature. The population of the study was the middle aged office employees, who are generally defined an age group from 40 to 60. The sample of this study was targeted to employees working at companies registered to securities, KSDAQ, and KONEX in Korea. SPSS WIN 23.0 and AMOS 21.0, and M-plus 6.12 were used for the data analysis. The findings of this study were presented below. First, the result of the structural relationship between the predictive factors of successful aging (subjective health condition, cognitive function, self-esteem, and social support) and successful aging was appropriate as RMSEA=.059, TLI=.932, CFI=.944. Second, subjective health condition and social support were significant variables for successful aging but cognitive function and self-esteem were not significant. Third, social support, which is a social factor, was the most influential among other variables. Based on the results, theoretical and practical implications were presented.

• Korean Journal of Agricultural and Forest Meteorology
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• v.24 no.4
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• pp.346-352
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• 2022

Mountain meteorology in South Korea that is covered mountains with complex terrain is important for understanding and managing the forest disaster and forest ecosystems. In particular, recent changes in dryness and/or rainfall intensity due to climate change may cause an increase in the possibility of forest disasters. Therefore, accurate monitoring of mountain meteorology is needed for efficient forest management. Korea Forest Service (KFS) is establishing the Automatic Mountain Meteorology Observation Stations (AMOS) in the mountain regions since 2012. 464 AMOSs are observing various meteorological variables such as air temperature, relative humidity, wind speed and direction, precipitation, soil temperature, and air pressure for every minute, which is conducted the quality control (QC) to retain data reliability. QC process includes the physical limit test, step test, internal consistency test, persistence test, climate range test, and median filter test. All of AMOS observations are open to use, which can be found from the Korean Mountain Meteorology Information System (KoMIS, http://mtweather.nifos.go.kr/) of the National Institute of Forest Science and the Public Data Portal (https://public.go.kr/). AMOS observations with guaranteed quality can be used in various forest fields including the public safety, forest recreation, forest leisure activities, etc., and can contribute to the advancement of forest science and technology. In this paper, a series of processes are introduced to collect and use the AMOS dataset in the mountain region in South Korea.

• Korean Journal of Ecology and Environment
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• v.51 no.1
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• pp.105-123
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• 2018

Currently, satellite images act as essential and important data in water resources, environment, and ecology as well as information of geographic information system. In this paper, we will investigate basic characteristics of satellite images, especially application examples in water resources. In recent years, researches on spatial and temporal characteristics of large-scale regions utilizing the advantages of satellite imagery have been actively conducted for fundamental hydrological components such as evapotranspiration, soil moisture and natural disasters such as drought, flood, and heavy snow. Furthermore, it is possible to analyze temporal and spatial characteristics such as vegetation characteristics, plant production, net primary production, turbidity of water bodies, chlorophyll concentration, and water quality by using various image information utilizing various sensor information of satellites. Korea is planning to launch a satellite for water resources and environment in the near future, so various researches are expected to be activated on this field.

• Korean Journal of Remote Sensing
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• v.26 no.2
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• pp.65-73
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• 2010

To observe and analyze the characteristics of cloud and precipitation properties, the Cloud physics Observation System (CPOS) has been operated from December 2003 at Daegwallyeong ($37.4^{\circ}N$, $128.4^{\circ}E$, 842 m) in the Taebaek Mountains. The major instruments of CPOS are follows: Forward Scattering Spectrometer Probe (FSSP), Optical Particle Counter (OPC), Visibility Sensor (VS), PARSIVEL disdrometer, Microwave Radiometer (MWR), and Micro Rain Radar (MRR). The former four instruments (FSSP, OPC, visibility sensor, and PARSIVEL) are for the observation and analysis of characteristics of the ground cloud (fog) and precipitation, and the others are for the vertical cloud characteristics (http://weamod.metri.re.kr) in real time. For verification of CPOS products, the comparison between the instrumental products has been conducted: the qualitative size distributions of FSSP and OPC during the hygroscopic seeding experiments, the precipitable water vapors of MWR and radiosonde, and the rainfall rates of the PARSIVEL(or MRR) and rain gauge. Most of comparisons show a good agreement with the correlation coefficient more than 0.7. These reliable CPOS products will be useful for the cloud-related studies such as the cloud-aerosol indirect effect or cloud seeding. The visibility value is derived from the droplet size distribution of FSSP. The derived FSSP visibility shows the constant overestimation by 1.7 to 1.9 times compared with the values of two visibility sensors (SVS (Sentry Visibility Sensor) and PWD22 (Present Weather Detect 22)). We believe this bias is come from the limitation of the droplet size range ($2{\sim}47\;{\mu}m$) measured by FSSP. Further studies are needed after introducing new instruments with other ranges.

• Korean Journal of Agricultural and Forest Meteorology
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• v.19 no.3
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• pp.153-163
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• 2017

The objective of this study is to enhance the model's speed of estimating weather variables (e.g., minimum/maximum temperature, sunshine hour, PRISM (Parameter-elevation Regression on Independent Slopes Model) based precipitation), which are applied to the Agrometeorological Early Warning System (http://www.agmet.kr). The current process of weather estimation is operated on high-performance multi-core CPUs that have 8 physical cores and 16 logical threads. Nonetheless, the server is not even dedicated to the handling of a single county, indicating that very high overhead is involved in calculating the 10 counties of the Seomjin River Basin. In order to reduce such overhead, several cache and parallelization techniques were used to measure the performance and to check the applicability. Results are as follows: (1) for simple calculations such as Growing Degree Days accumulation, the time required for Input and Output (I/O) is significantly greater than that for calculation, suggesting the need of a technique which reduces disk I/O bottlenecks; (2) when there are many I/O, it is advantageous to distribute them on several servers. However, each server must have a cache for input data so that it does not compete for the same resource; and (3) GPU-based parallel processing method is most suitable for models such as PRISM with large computation loads.