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Linkage Map and Quantitative Trait Loci(QTL) on Pig Chromosome 6 (돼지 염색체 6번의 연관지도 및 양적형질 유전자좌위 탐색)

  • Lee, H.Y.;Choi, B.H.;Kim, T.H.;Park, E.W.;Yoon, D.H.;Lee, H.K.;Jeon, G.J.;Cheong, I.C.;Hong, K.C.
    • Journal of Animal Science and Technology
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    • v.45 no.6
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    • pp.939-948
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    • 2003
  • The objective of this study was to identify the quantitative traits loci(QTL) for economically important traits such as growth, carcass and meat quality on pig chromosome 6. A three generation resource population was constructed from cross between Korean native boars and Landrace sows. A total of 240 F$_2$ animals were produced using intercross between 10 boars and 31 sows of F$_1$ animals. Phenotypic data including body weight at 3 weeks, backfat thickness, muscle pH, shear force and crude protein level were collected from F$_2$ animals. Animals including grandparents(F$_0$), parents(F$_1$) and offspring(F$_2$) were genotyped for 29 microsatellite markers and PCR-RFLP marker on chromosome 6. The linkage analysis was performed using CRI-MAP software version 2.4(Green et al., 1990) with FIXED option to obtain the map distances. The total length of SSC6 linkage map estimated in this study was 169.3cM. The average distance between adjacent markers was 6.05cM. For mapping of QTL, we used F$_2$ QTL Analysis Servlet of QTL express, a web-based QTL mapping tool(http://qtl.cap.ed.ac.uk). Five QTLs were detected at 5% chromosome-wide level for body weight of 3 weeks of age, shear force, meat pH at 24 hours after slaughtering, backfat thickness and crude protein level on SSC6.

An Introduction of Korean Soil Information System (한국 토양정보시스템 소개)

  • Hong, S. Young;Zhang, Yong-Seon;Hyun, Byung-Keun;Sonn, Yeon-Kyu;Kim, Yi-Hyun;Jung, Sug-Jae;Park, Chan-Won;Song, Kwan-Cheol;Jang, Byoung-Choon;Choe, Eun-Young;Lee, Ye-Jin;Ha, Sang-Keun;Kim, Myung-Suk;Lee, Jong-Sik;Jung, Goo-Bok;Ko, Byong-Gu;Kim, Gun-Yeob
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.1
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    • pp.21-28
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    • 2009
  • Detailed information on soil characteristics is of great importance for the use and conservation of soil resources that are essential for human welfare and ecosystem sustainability. This paper introduces soil inventory of Korea focusing on national soil database establishment, information systems, use, and future direction for natural resources management. Different scales of soil maps surveyed and soil test data collected by RDA (Rural Development Administration) were computerized to construct digital soil maps and database. Soil chemical properties and heavy metal concentrations in agricultural soils including vulnerable agricultural soils were investigated regularly at fixed sampling points. Internet-based information systems for soil and agro-environmental resources were developed based on 'National Soil Survey Projects' for managing soil resources and for providing soil information to the public, and 'Agroenvironmental Change Monitoring Project' to monitor spatial and temporal changes of agricultural environment will be opened soon. Soils data has a great potential of further application in estimation of soil carbon storage, water capacity, and soil loss. Digital mapping of soil and environment using state-of-the-art and emerging technologies with a pedometrics concept will lead to future direction.

Effects of Perceived Similarity between Consumers and Product Reviewers on Consumer Behaviors (상품후기 작성자에 대해 상품후기 독자가 느끼는 유사성이 상품후기 독자에게 미치는 영향)

  • Kim, Ji-Young;Suh, Eung-Kyo;Suh, Kil-Soo
    • Asia pacific journal of information systems
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    • v.18 no.3
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    • pp.67-90
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    • 2008
  • Prior to making choices among online products and services, consumers often search online product reviews written by other consumers. Online product reviews have great influences on consumer behavior because they are believed to be more reliable than information provided by sellers. However, ever-increasing lists of product reviews make it difficult for consumers to find the right information efficiently. A customized search mechanism is a method to provide personalized information which fits the user's requirements. This study examines effects of a customized search mechanism and perceived similarity between consumers and product reviewers on consumer behaviors. More specifically, we address the following research questions: (1) Can a customized search mechanism increase perceived similarity between product review authors and readers? (2) Are product reviews perceived as more credible when product reviews were written by the authors perceived similar to them? (3) Does credibility of product reviews have a positive impact on acceptance of product reviews? (4) Does acceptance of product reviews have an influence on purchase intention of the readers? To examine these research questions, a lab experiment with a between-subject factor (whether a customized search mechanism is provided or not) design was employed. In order to enhance mundane realism and increase generalizability of the findings, the experiment sites were built based on a real online store, cherrya.com (http://www.cherrya.com/). Sixty participants were drawn from a pool that consisted of undergraduate and graduate students in a large university. Participation was voluntary; all the participants received 5,000 won to encourage their motivation and involvement in the experiment tasks. In addition, 15 participants, who selected by a random draw, received 30,000 won to actually purchase the product that he or she decided to buy during the experiment. Of the 60 participants, 25 were male and 35 were female. In examining the homogeneity between the two groups, the results of t-tests revealed no significant difference in gender, age, academic years, online shopping experience, and Internet usage. To test our research model, we completed tests of the measurement models and the structural models using PLS Graph version 3.00. The analysis confirmed individual item reliability, internal consistency, and discriminant validity of measurements. The results show that participants feel more credible when product reviews were written by the authors perceived similar to them, credibility of product reviews have a positive impact on acceptance of product reviews, and acceptance of product reviews have an influence on purchase intention of the readers. However, a customized search mechanism did not increase perceived similarity between product review authors and readers. The results imply that there is an urgent need to develop a better customized search tool in order to increase perceived similarity between product review authors and readers.

Present Status and Clinical Study Trend of Moxibustion in Korea Medicine (국내 뜸 연구의 현황과 경향)

  • Park, Sun-Young;Park, Sun-Ju;Park, Jung-Su;Ko, Seong-Gyou;Kong, Kyung-Whan;Shin, Mi-Ran;Jun, Chan-Yong;Jung, Hee;Lee, Myung-Su;Kim, Ho-Hyun;Go, Ho-Yeon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.6
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    • pp.1061-1068
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    • 2011
  • Moxibustion is a treatment method for cold and pain in Korean medicine. But the systematic study of moxibustion were very few. This study was aimed to survey and evaluate moxibustion study in Korean medicine. We search the moxibustion study in journals related Korean medicine and http://oasis.kiom.re.kr. The period of the study from first issue to August, 2010. The search word were moxibustion, moxa in keyword and moxibustion treatment in title. We totally search 334 articles. But 175 articles were excluded because this study were not exactly moxibustion study, so we included and analyzed 159 articles. The study of moxibustion were 159 articles. Literature review were 35 articles, systematic review 8, heat experiment 10, in vivo or vitro 28 and survey investigation 3. Clinical articles of moxibustion related to Korean medical journals were 76. Before and after study were 31 articles, case report 20, cohort study 7, cross-sectional study 2, nonrandomized study 6, quasi randomized study 1, randomized clinical trials 9. This results showed that moxibustion study were smaller than acupuncture or herb and concentrated to pain and cold syndrome. We need further larger and diverse study of moxibustion.

The establishment of orthodontic web server multimedia database system for continuing education (임상교정 교육용 멀티미디어 데이터베이스 웹서버 구축에 관한 연구)

  • Park, Jae-Woo;Lee, Jong-Ki;Chang, Young-Il;Nahm, Dong-Suk;Kim, Myung-Ki;Yang, Won-Sik;Kim, Tae-Woo;Baek, Seung-Hak
    • The korean journal of orthodontics
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    • v.30 no.2 s.79
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    • pp.245-260
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    • 2000
  • The Objective of this research was to construct the multimedia database system that was necessary for the education of the practitioners and the students. To establish this system, there were technical problems as follows 1) The processing of the images, 2) The rapid processing of the information with the computer network, 3) The development of diagnosis tools, 4) The technique to establish the database system, 5) The link between the database system and the internet, and 6) The method to educate through many cases. The analysis for diagnosis and treatment planning were provided as two parts : model analysis and cephalometric analysis. As a model analysis, arch length discrepancy and Bolton tooth ratio were provided for the part of patient information. Cephalometric analyses were provided in the part of initial diagnosis. The Cephalometric analysis ver 2.0 and the PowerCeph pro 3.3.5 were used to show Tweed, Steiner and Jarabak analysis. In the main part, Kim's analysis and some measurements were added. In the post-treatment or retention part, we show the superimposition of the cephalometrics with which you can find the effectiveness of the various orthodontic treatment The address of this home page is "http://damis.snu.ac.kr/orthodontics"

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Manual of River Corridor Survey and Monitoring for Nature-Friendly River Management (자연 친화적 하천관리를 위한 수변조사 및 모니터링 매뉴얼)

  • Ock Giyoung;Woo Hyoseop;Kim Kyuho;Cho Kanghyun
    • Proceedings of the Korea Water Resources Association Conference
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    • 2005.05b
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    • pp.1269-1273
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    • 2005
  • 자연 친화적 하천관리는 현 치수위주의 하천정비 관행으로 인한 하천의 환경 기능의 훼손을 막고, 제도적으로 자연 친화적으로 하천 사업을 추진하기 위해서 수변조사, 계획, 설계, 시공, 모니터링, 그리고 유지관리 등 일련의 체계적인 절차에 따라 하천 사업을 시행하는 것이다. 특히 이러한 표준 절차 가운데 수변조사는 하천의 특성을 결정짓는 여러 가지 자연적, 인공적 형성과정들과 그 과정들을 지배하는 생태학적 원리들을 파악하여 하천사업의 정비주제와 방향을 설정하는데 도움을 준다. 모니터링은 계획과 설계, 시공을 통해 실시된 하천사업의 효과를 평가하고, 공법적용에 따른 하천의 변화과정을 파악하여 유지관리 및 적응관리를 위한 근거를 제시하는 과정이다. 수변조사 및 모니터링 매뉴얼은 건설교통부가 수행한 '자연친화적 하천정비기법 개발' 의 연구성과로서, 경기도의 탄천, 충청북도의 달천 그리고 경기도 오산천을 대상으로 각 관련분야의 전문가가 참여하여 직접 적용$\cdot$검증한 결과를 바탕으로 제작한 것이다. 이는 하천의 관리 및 하천관련 사업을 자연 친화적으로 수행하기 위하여 필요한 '수변조사'와 '모니터링'에 대한 일반적인 절차와 방법을 체계화 한 것이다. 특히 수변조사 매뉴얼의 경우는 $\ulcorner$하천설계기준$\lrcorner$의 '제12장 하천환경조사'를 보완하는 관계에 있다. 하천설계기준에서 제시한 하천환경조사는 본 수변조사 매뉴얼상과 동일한 절차와 양식을 따르고 있다. 하천설계기준에서 자세하게 기술하지 못한 조사방법, 정리, 분석, 평가에 대한 내용을 구체적으로 적용하여 이를 보완하고 있다.은 안양천 웹페이지(http://anyang.river.or.kr)에서 구현되고 있으며, 앞서 설명한 바와 같이 1단계 프론티어 사업으로 설치된 4개의 하천수위, 2개의 지하수위 관측시설과 함께, 2단계에 (주)웹솔루스에서 자체적으로 설치 운영하고 있는 2개소의 하수관거 모니터링 관측시설, 그리고 안양시에서 운영하고 있는 5개소의 강우관측소와 7개소의 수위관측소를 모두 통합하여 실시간 자료를 제공하고 있다. 수위자료는 10분단위의 텍스트정보와 그래프형태로 지원되며, 검색기간 설정을 통해 원하는 기간내의 자료를 선별, 검색할 수 있다.. 또한 이와 같은 기초적인 정보를 바탕으로 하류하천의 탁수 피해를 최소화할 수 있는 선택취수탑의 운영방안을 수립할 수 있다 본 연구에서는 이를 위해 선택취수탑 주위의 성층흐름을 기존의 실험자료와 수치해석을 통하여 분석하였고, 온도성층구조나 취수구의 위치변화에 따른 방류수 수질특성을 조사하였다.쇄파대(artifical reef)와 같은 완충지대를 갖는 호안을 축조함으로써 월파량을 감소시키는 대안으로 제시하고자 한다. 본 연구 수행을 통해 태풍 내습시 발생 가능한 자연재해에 대한 사전 방지를 목적으로 태풍피해의 원인을 제시하고 이를 해결하여 현재의 방재대책이 항구적인 방재대책으로 전환될 수 있는 방안 마련의 기초 자료로 활용되기를 기대한다., L-arabinose, 및 D-galactose; 제3차(第三次) 가수분해물(加水分解物)(C)에서 L-rhamnose, D-xylose, L-arabinose 및 D-galactose, 비가수분해물(非加水分解物)(C')에서 D-xylose와 D-galactose를 검출(檢出)하였다. (4) 구성당(構

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Molecular Cloning and Bioinformatic Analysis of SPATA4 Gene

  • Liu, Shang-Feng;Ai, Chao;Ge, Zhong-Qi;Liu, Hai-Luo;Liu, Bo-Wen;He, Shan;Wang, Zhao
    • BMB Reports
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    • v.38 no.6
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    • pp.739-747
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    • 2005
  • Full-length cDNA sequences of four novel SPATA4 genes in chimpanzee, cow, chicken and ascidian were identified by bioinformatic analysis using mouse or human SPATA4 cDNA fragment as electronic probe. All these genes have 6 exons and have similar protein molecular weight and do not localize in sex chromosome. The mouse SPATA4 sequence is identified as significantly changed in cryptorchidism, which shares no significant homology with any known protein in swissprot databases except for the homologous genes in various vertebrates. Our searching results showed that all SPATA4 proteins have a putative conserved domain DUF1042. The percentages of putative SPATA4 protein sequence identity ranging from 30% to 99%. The high similarity was also found in 1 kb promoter regions of human, mouse and rat SPATA4 gene. The similarities of the sequences upstream of SPATA4 promoter also have a high proportion. The results of searching SymAtlas (http://symatlas.gnf.org/SymAtlas/) showed that human SPATA4 has a high expression in testis, especially in testis interstitial, leydig cell, seminiferous tubule and germ cell. Mouse SPATA4 was observed exclusively in adult mouse testis and almost no signal was detected in other tissues. The pI values of the protein are negative, ranging from 9.44 to 10.15. The subcellular location of the protein is usually in the nucleus. And the signal peptide possibilities for SPATA4 are always zero. Using the SNPs data in NCBI, we found 33 SNPs in human SPATA4 gene genomic DNA region, with the distribution of 29 SNPs in the introns. CpG island searching gives the data about CpG island, which shows that the regions of the CpG island have a high similarity with each other, though the length of the CpG island is different from each other.This research is a fundamental work in the fields of the bioinformational analysis, and also put forward a new way for the bioinformatic analysis of other genes.

Antioxidant capacity in seedling of colored-grain wheat under water deficit condition

  • Kim, Dae Yeon;Hong, Min Jeong;Jung, Woo Joo;Seo, Yong Weon
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.140-140
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    • 2017
  • Nutritious and functional foods from crop have received great attention in recent years. Colored-grain wheat contains high phenolic compound and a large number of flavonoid. The anthocyanin and polyphenolic synthesis and accumulation is generally stimulated in response to biotic or abiotic stresses. Here, we analyzed genome wide transcripts in seedling of colored-grain wheat response to ABA and PEG treatment. About 900 and 1500 transcripts (p-value < 0.05) from ABA and PEG treatment were aligned to IWGSC1+popseq DB which is composed of over 110,000 transcripts including 100,934 coding genes. NR protein sequences of Poaceae from NCBI and protein sequence of transcription factors originated from 83 species in plant transcription factor database v3.0 were used for annotation of putative transcripts. Gene ontology analysis were conducted and KEGG mapping was performed to show expression pattern of biosynthesis genes related in flavonoid, isoflavonoid, flavons and anthocyanin biopathway. DroughtDB (http://pgsb.helmholtz-muenchen.de/droughtdb/) was used for detection of DEGs to explain that physiological and molecular drought avoidance by drought tolerance mechanisms. Drought response pathway, such as ABA signaling, water and ion channels, detoxification signaling, enzymes of osmolyte biosynthesis, phospholipid metabolism, signal transduction, and transcription factors related DEGs were selected to explain response mechanism under water deficit condition. Anthocyanin, phenol compound, and DPPH radical scavenging activity were measured and antioxidant activity enzyme assays were conducted to show biochemical adaptation under water deficit condition. Several MYB and bHLH transcription factors were up-regulated in both ABA and PEG treated condition, which means highly expressed MYB and bHLH transcription factors enhanced the expression of genes related in the biosynthesis pathways of flavonoids, such as anthocyanin and dihydroflavonols in colored wheat seedlings. Subsequently, the accumulation of total anthocyanin and phenol contents were observed in colored wheat seedlings, and antioxidant capacity was promoted by upregulation of genes involved in maintaining redox state and activation of antioxidant scavengers, such as CAT, APX, POD, and SOD in colored wheat seedlings under water deficit condition. This work may provide valuable and basic information for further investigation of the molecular responses of colored-grain wheat to water deficit stress and for further gene-based studies.

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Rice Proteomics: A Functional Analysis of the Rice Genome and Applications (프로테옴 해석에 의한 벼 게놈 기능해석과 응용)

  • Woo, Sun-Hee;Kim, Hong-Sig;Song, Berm-Heun;Lee, Chul-Won;Park, Young-Mok;Jong, Seung-Keun;Cho, Yong-Gu
    • Journal of Plant Biotechnology
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    • v.30 no.3
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    • pp.281-291
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    • 2003
  • In this review, we described the catalogues of the rice proteome which were constructed in our program, and functional characterization of some of these proteins was discussed. Mass-spectrometry is the most prevalent technique to rapidly identify a large number of proteome analysis. However, the conventional Western blotting/sequencing technique has been used in many laboratories. As a first step to efficiently construct protein cata-file in proteome analysis of major cereals, we have analyzed the N-terminal sequences of 100 rice embryo proteins and 70 wheat spike proteins separated by two-dimensional electrophoresis. Edman degradation revealed the N-terminal peptide sequences of only 31 rice proteins and 47 wheat proteins, suggesting that the rest of separated protein sports are N-terminally blocked. To efficiently determine the internal sequence of blocked proteins, we have developed a modified Cleveland peptide mapping method. Using this above method, the internal sequences of all blocked rice proteins(i, e., 69 proteins) were determined. Among these 100 rice proteins, thirty were proteins for which homologous sequence in the rice genome database could be identified. However, the rest of the proteins lacked homologous proteins. This appears to be consistent with the fact that about 45% of total rice cDNA have been deposited in the EMBL database. Also, the major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that tuned out to be calreticulin, gibberellin-binding protein, which is ribulose-1.5-bisphosphate carboxylase/oxygense active in rice, and leginsulin-binding protein in soybean have functions in the signal transduction pathway. Proteomics is well suited not only to determine interaction between pairs of proteins, but also to identify multisubunit complexes. Currently, a protein-protein interaction database for plant proteins(http://genome.c.kanazawa-u.ac.jp/Y2H)could be a very useful tool for the plant research community. Also, the information thus obtained from the plant proteome would be helpful in predicting the function of the unknown proteins and would be useful be in the plant molecular breeding.

An Analysis of the Effectiveness of Stroke between East-West Integrative Medicine Hospital and Western Medicine Hospital by the Data of Brain Rehabilitation Registry (뇌질환 재활 통합 등록체계 자료(Brain Rehabilitation Registry)를 통한 한양방 협진병원과 양방병원의 뇌졸중 환자 치료효과 분석)

  • Heo, Kwang-Ho;Hwang, Eui-Hyoung;Cho, Hyun-Woo;Lee, In;Hong, Jin-Woo;Shin, Yong-Il;Kim, Soo Yeon;Shin, Byung-Cheul
    • Journal of Korean Medicine Rehabilitation
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    • v.23 no.3
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    • pp.117-124
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    • 2013
  • Objectives The aims of this study was to observe the effectiveness of East-West Integrative Medicine (EWIM) by analysing between EWIM estimated group and Non-EWIM eastimated group with Brain Rehabilitation Registry. Methods We divided hospitals into two groups, one is EWIM estimated group hospital (Kyeonghee university, Wonkwang university, Dongguk university) and the other is non-EWIM estimated group (Jeonnam university, Gyeongbuk university, Catholic university). Then we analysed the effectiveness of treatments retrospectively using the database of Brain Rehabilitation Registry (http://www.kbrr.or.kr/, version 1.0). Totally 293 patients were included, EWIM estimated group was 175 and non-EWIM estimated group was 118. The main outcome measurements were National Institutes of Health Stroke Scale (NIHSS), Modified Barthel Index (MBI) and Mini Mental State Examination-Korea (MMSE-K). Results Changes of NIHSS was not significant in both total patient and patient who treated over 3 weeks. MMSE-K showed positively significant difference (p=0.044) in EWIM estimated group patients who treated over 3 weeks. In case of MBI, EWIM estimated group showed more effective result and also statistically significant in both total patients and patients who treated over 3 weeks. Conclusions We patially argued that EWIM estimated group was more effective than non-EWIM estimated group in stroke patients' functional recovery. We suggested base data of EWIM in stroke patiens through this study and this could be applied future researches of developing modified EWIM system.