• Biomolecules & Therapeutics
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• v.31 no.4
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• pp.402-410
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• 2023

Long-term administration of levodopa (L-DOPA) to patients with Parkinson's disease (PD) commonly results in involuntary dyskinetic movements, as is known for L-DOPA-induced dyskinesia (LID). 5-Hydroxytryptophan (5-HTP) has recently been shown to alleviate LID; however, no biochemical alterations to aberrant excitatory conditions have been revealed yet. In the present study, we aimed to confirm its anti-dyskinetic effect and to discover the unknown molecular mechanisms of action of 5-HTP in LID. We made an LID-induced mouse model through chronic L-DOPA treatment to 6-hydroxydopamine-induced hemi-parkinsonian mice and then administered 5-HTP 60 mg/kg for 15 days orally to LID-induced mice. In addition, we performed behavioral tests and analyzed the histological alterations in the lesioned part of the striatum (ST). Our results showed that 5-HTP significantly suppressed all types of dyskinetic movements (axial, limb, orolingual and locomotive) and its effects were similar to those of amantadine, the only approved drug by Food and Drug Administration. Moreover, 5-HTP did not affect the efficacy of L-DOPA on PD motor manifestations. From a molecular perspective, 5-HTP treatment significantly decreased phosphorylated CREB and ΔFosB expression, commonly known as downstream factors, increased in LID conditions. Furthermore, we found that the effects of 5-HTP were not mediated by dopamine1 receptor (D1)/DARPP32/ERK signaling, but regulated by AKT/mTOR/S6K signaling, which showed different mechanisms with amantadine in the denervated ST. Taken together, 5-HTP alleviates LID by regulating the hyperactivated striatal AKT/mTOR/S6K and CREB/ΔFosB signaling.

• Nutrition Research and Practice
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• v.12 no.3
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• pp.208-214
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• 2018

BACKGROUND/OBJECTIVES: The aim of this study was to evaluate the biological and sleep-promoting effects of combined ${\gamma}$-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) using caffeine-induced sleepless fruit flies, ICR mice, and Sprague-Dawley rats. MATERIALS/METHODS: Video-tracking analysis was applied to investigate behavioral changes of Drosophila melanogaster. Pentobarbital-induced sleep test and electroencephalogram (EEG) patterns were used for analysis of sleep latency, duration, and quantity and quality of sleep in vertebrate models. RESULTS: Administration of combined GABA/5-HTP could significantly reverse the caffeine induced total distance of flies (P < 0.001). Also, individually administered and combined GABA/5-HTP significantly increased the total sleeping time in the caffeine-induced sleepless ICR mice (P < 0.001). In the caffeine-induced sleepless SD-rats, combined GABA/5-HTP showed significant differences in sleep quality between individual amino acid administrations (P < 0.05). CONCLUSIONS: Taken together, we identified inhibitory effects of combined GABA/5-HTP in locomotor activity, sleep quantity and quality in caffeine-induced sleepless models, indicating that combined GABA/5-HTP may be effective in patients with insomnia by providing sufficient sleep.

• Food Science of Animal Resources
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• v.37 no.5
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• pp.646-653
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• 2017

Alcalase hydrolysis of liquid egg white was used to produce 5-hydroxytryptophan (HTP) under various conditions and investigate the sleep-potentiating activity of liquid egg white hydrolysate (LEH) on pentobarbital-induced sleep. Alcalase hydrolysis yielded the highest content of 5-HTP ($13.50{\mu}g/mL$), while neutrase hydrolysis showed the lowest 5-HTP content ($5.23{\mu}g/mL$). The liquid egg white to water ratio (1:1) was optimal for the production of 5-HTP with high amino-nitrogen (A-N) content and degree of hydrolysis. The 5-HTP, amino-nitrogen, and degree of hydrolysis increased until 24 h of hydrolysis and slightly increased thereafter during hydrolysis with 2% and 5% enzyme addition. 5-HTP administration at doses of 6 and 9 mg/kg significantly increased sleep duration and decreased sleep latency time compared to that in the control (p<0.05). LEH (150 mg/mouse), which was equivalent to 5-HTP at 6 mg/kg, significantly decreased sleep latency time and increased sleep duration time compared to that in the control (p<0.05). Oral administration of LEH showed sleep-potentiating effects because of 5-HTP. The sleep-potentiating activity of LEH may have occurred through 5-HTP in our pentobarbital-induced sleep model. LEH may be a valuable alternative to sleep enhancement and may be used as a sleep-potentiating agent.

• Proceedings of the Korea Information Processing Society Conference
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• 2021.11a
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• pp.558-561
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• 2021

심리학에서 사람의 심리 상태를 알아보기 위해 사용되는 검사 방법 중, 집-나무-사람 검사(HTP Test)는 피실험자가 그린 집, 나무, 사람을 포함하는 그림을 사용하여 피실험자의 심리를 분석하는 투영 검사법이다. 본 논문에서는 딥러닝 모델을 이용해 HTP Test 에 사용되는 그림을 분석하는 시스템을 제안하며, 성능 평가를 통해 심리학에서의 딥러닝 모델 적용 가능성을 확인한다. 또한 그림 데이터 분석에 적합한 사전 훈련 모델을 개발하기 위해, ImageNet 과 스케치 데이터셋으로 사전 훈련하여 성능을 비교한다. 본 논문에서 제안하는 시스템은 크게 감정 분석을 위한 이미지 객체 추출부, 추출된 객체로 피실험자의 감정을 분류하는 감정 분류부로 구성되어 있다. 객체 추출과 이미지 분류 모두 CNN(Convolution Neural Network) 기반의 딥러닝 모델을 사용하며, 이미지 분류 모델은 서로 다른 데이터셋으로 모델을 사전 훈련한 후, 훈련 데이터셋으로 전이 학습하여 모델의 성능을 비교한다. 그림 심리 분석을 위한 HTP test 스케치 데이터셋은, HTP Test 와 동일하게 피실험자가 3 개 클래스의 집, 나무, 사람의 그림을 그려 자체 수집하였다.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.11B
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• pp.999-1006
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• 2003

In the paper, we investigate the impact of handoff on the packet delivery in the Mobile IPv6 networks, where the smooth handoff is adopted. That is measured by an 'unstable time period (UTP)', a 'silence time period(STP)', and a 'handoff time period (HTP)' in the mobile node's perspective. Then, we propose a queuing model to get the exact value of the handoff transient time. The numerical results show that the queuing delay for the handoff packets and the involved link (or route) capacities affect the estimated UTP, STP, and HTP. On the other hand, the damage of application caused by handoff will increases when the handoff transient time becomes longer. We show that the priority scheduling method can achieve shorter STP and UP than the FIFO scheduling method that is generally used in best-effort IP network.

• Educational Technology International
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• v.10 no.1
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• pp.107-135
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• 2009

The purpose of this paper is to review a variety of challenges facing the Human Performance Technology (HPT) in supporting knowledge workers' performance, and to explore possible HPT models for knowledge workers. The first section of this paper investigates the core attributes and major models of HPT as a foundation. While HPT has a lot of strengths in terms of systemic, systematic, methodologically eclectic, evidence based, and results oriented approaches, some pitfalls - which could be detected if these principles were mindlessly applied to problem areas - are explored. The second section presents some considerations such as analysis, intervention design, and leadership that HP technologists need to take in order to make HPT a better field of practice for knowledge workers. The author also suggests a tentative diagnostic model and a process model for knowledge workers, core principles of which are based on systems thinking, in particular Senge's the fifth discipline and Checkland's soft systems methodology. The importance of formative evaluations to improve these models is noted as a conclusion.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.15 no.1
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• pp.61-74
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• 2004

Objectives：This study was performed to introduce a psychoeducational family therapy model for the families of schizophrenic patient and to investigate the effect of this model on the changes in coping style and depressive symptoms of the family members, and in perception of emotional support by families and depressive symptoms of patients. Methods：Nine preschool children, 3-5 years old, experiencing physical injuries caused by attack from a psychotic patient at kindergarten, were evaluated for psychological assessments；Intelligence test, MSSB(MacArthur Story-Stem Battery), H-T-P test(House-Tree-Person test). And their parents completed rating scale, KPI-C(Korean Personality Inventory for Children about children’s psychological conditions). Results：With respects to the contents and emotional reactions of MSSB, 9 preschool children showed generally high levels of anxiety, depression, avoidance, aggression, probably related to the traumatic experiences. Even though children couldn't verbally report directly about their traumatic experiences, in both MSSB, structured play narrative assessment tool, and HPT, free drawing and association test, they demonstrated psychiatric problems through reenactment plays, regardless of clinical diagnoses. Conclusion：Present study allowed us the chance to see beyond the outer pathological behaviors of PTSD in preschool children, through deeper evaluations of their mental representation. These preliminary data suggest deep understanding of internal representation would be of help for thorough evaluations and treatment plan for preschool children, experiencing severe trauma.

• The KIPS Transactions:PartC
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• v.9C no.4
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• pp.605-614
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• 2002

Amount of the web traffic web server handles are explosively increasing, which requires that the performance of the web server should be improved for the various web services. Although the analysis for the HTTP traffic with the proper tuning for the web server is essential, the research relevant to the subject are insignificant. In particular, although most of applications are implemented over HTTP 1.1 protocol, the researches mostly deal with the performance evaluation of the HTTP 1.0 protocol. Consequently, the modeling approach and the performance evaluation over HTTP 1.1 protocol have not been well formed. Therefore, basing on the HTTP 1.1 protocol supporting persistent connection, we present an analytical end-to-end tandem queueing model for web server to consider the specific hardware configuration inside web server beginning at accepting the user request until completing the service. we compare various performances between HTTP 1.0 and HTTP 1.1 under the overloading condition, and then analyze the characteristics of the HTTP traffic that include file size requested to web server, the OFF time between file transfers, the frequency of requests, and the temporal locality of requests. Presented model is verified through the comparing the server throughput according to varying requests rate with the real web server. Thereafter, we analyze the performance evaluation of the web server, according to the interrelation between TCP Listen queue size, the number of HTTP threads and the size of the network buffers.