• Childhood Kidney Diseases
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• 제2권2호
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• pp.118-124
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• 1998

목 적 : HS 신염은 확정된 특별한 치료지침이 없으며, 신염의 정도가 심한 경우 단일 스테로이드 요법으로 치료 효과가 좋지 않으므로 장기간 스테로이드 투여 및 azathioprine의 병합에 의한 치료 효과를 관찰하기 위해 본 연구를 시행하였다. 대상 및 방법 : Henoch-$Sch{\ddot{o}}nlein$ purpura(HSP)로 진단 받은 후 심한 단백뇨(40 mg/hr/$m^2$ 이상)를 보인 HS 신염 환아 5례(남자 2례, 여자 3례)를 대상으로 신장 생검을 실시하여 ISKDC grade III 이상을 보인 환아를 대상으로 하였고 치료방법은 1)스테로이드 pulse치료를 격일로 6회 정주한 다음, 2) 경구로 스테로이드를 2mg/kg/day(최대 80mg/day) 1개월 투여한 후 1 mg/kg/day으로 1 개월 투여하고 2년간 격일로 1mg/kg/day경구 투여하였다. 3)스테로이드 pulse치료 시행 뒤 azathioprine(2 mg/kg/day)을 2년간 병합하여 경구 투여하였다. 단백뇨 및 미세 혈뇨를 추적 검사하여 임상적 관해 여부를 조사하였고 추적 신장조직 검사를 시행하였다. 결 과 :발병 평균 연령은 $10.5{\pm}3.4$년이었으며 HSP에서 신염으로의 발병 기간은 2주에서 5개월로 평균 $7.4{\pm}7.4$주이었다. 24시간 뇨단백은 평균 $4857.8{\pm}2046.1$ mg/day이었다. 임상적 관해는 5례 중 4례($80\%$)로 단백뇨 소실의 평균기간은 $5{\pm}2.4$개월, 미세혈뇨의 소실 기간은 평균 $13.3{\pm}2.9$개월이었다. 병리학적 소견은 ISKDC 분류로 Crade IIIb가 3례, Grade VI가 2례로 추적 신장 조직 검사를 시행한 4례 중 3례에서 조직학적으로 호전된 양상을 보였다. 결 론 : 아직까지 HS 신염에 대한 확정된 치료 지침이 없는 상황에서 본 연구의 장기간 스테로이드 경구 투여 및 azathioprine의 병합 요법이 임상적, 조직학적으로 효과가 있는 것으로 생각되며 앞으로 더 많은 환아에서 추적 관찰이 필요하다.

• 대한의생명과학회지
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• 제9권3호
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• pp.159-165
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• 2003

Ankyrins are a ubiquitously expressed family of intracellular adaptor proteins involved in targeting diverse proteins to specialized membrane domains in both the plasma membrane and the endoplasmic reticulum. Recently, the studies with C-terminus of ankyrins have identified that ankyrin-B is capable of interacting with Hsp40 and sAnkl is capable of interacting with obscurin and titin, but the function of C-terminal domain of ankyrin-G remains unknown. To identify proteins interacting C-terminus of ankyrin-G, we used the C-terminus of ankyrin-G as a bait for a yeast two-hybrid screen of brain cDNA library. Approximately 1.33$\times$l0$^6$ transformants were screened, of which 13 positive clones were obtained as determined by activation of HIS3, ADE2 and MELl reporter genes. Sequence analyses of these 13 plasmids revealed that cDNA inserts of 13 colonies showed highly homologous to 11 genes, including 5 known (i.e., Na$^+$/K$^+$ ATPase $\beta$1, SERBPl, UTF2, cytochrome C oxidase and collagen IV $\alpha$2) and 6 unknown genes. The evaluation of the proteins that emerge from these experiments provides a rational approach to investigate the those proteins significant in interaction with ankyrin-G.

• Molecules and Cells
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• 제41권6호
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• pp.562-574
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• 2018

The tazarotene-induced gene 1 (TIG1) protein is a retinoidinducible growth regulator and is considered a tumor suppressor. Here, we show that DnaJ heat shock protein family member C8 (DNAJC8) is a TIG1 target that regulates glycolysis. Ectopic DNAJC8 expression induced the translocation of pyruvate kinase M2 (PKM2) into the nucleus, subsequently inducing glucose transporter 1 (GLUT1) expression to promote glucose uptake. Silencing either DNAJC8 or PKM2 alleviated the upregulation of GLUT1 expression and glucose uptake induced by ectopic DNAJC8 expression. TIG1 interacted with DNAJC8 in the cytosol, and this interaction completely blocked DNAJC8-mediated PKM2 translocation and inhibited glucose uptake. Furthermore, increased glycose uptake was observed in cells in which TIG1 was silenced. In conclusion, TIG1 acts as a pivotal repressor of DNAJC8 to enhance glucose uptake by partially regulating PKM2 translocation.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.235-239
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• 2004

Drosophila melanogaster heat shock protein 70 gene promoter (Dhsp70p) is widely used in transgenic insect to drive exogenous gene, and the homologous region 3 from Bombyx mori nucleopolyhedrovirus (BmNPVhr3) functions as an enhancer for several promoters. To test whether BmNPVhr3 can enhance the Dhsp70ps transcriptional activity, the reporter plasmids, which contain the Dhsp70p, the reporter $\beta$-galactosidase gene with SV40 terminator and BmNPVhr3 fragment, are constructed and transfected into the insect cell lines (Bm-N cells and Sf-21 cells) by lipofectin-mediated method. The results from the transient expression assay show that BmNPVhr3 significantly increases transcriptional activity of Dhsp70p both under the normal condition and under the heat-shock treatment, although the effects are significantly different between in Bm-N cells and in sf-21 cells. The enhancing behavior of BmNPVhr3 on the Dhsp70p is in an orientation-independent manner. Meanwhile, the effects of heat-shock treatment on Dhsp70p alone or Dhsp70p/BmNPVhr3 combination present no significant difference, indicating that BmNPVhr3 only enhances the transcriptional activity of Dhsp70p, but cant alter its characteristic of the response to the heat-shock stress. The above results suggest that the Dhsp70p/BmNPVhr3 combination is more effective one to drive exogenous gene for transgene or stable cell expression system in insects.

• 대한한의학방제학회지
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• 제31권4호
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• pp.295-313
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• 2023

Objectives : While treatments for cancer are advancing, the development of effective treatments for cancer metastasis, the main cause of cancer patient death, remains insufficient. Recent studies on Dichroae Radix have revealed that its active ingredients have the potential to inhibit cancer metastasis. This study aimed to investigate the cancer metastasis inhibitory effect of Dichroae Radix using network pharmacological analysis. Methods : The active compounds of Dichroae Radix have been identified using Traditional Chinese Medicine System Pharmacology Database and Analysis Platform. The UniProt database was used to collect each of information of all target proteins associated with the active compounds. To find the bio-metabolic processes associated with each target, the DAVID6.8 Gene Functional classifier tool was used. Compound-Target and Target-Pathway networks were analyzed via Cytoscape 3.40. Results : In total, 25 active compounds and their 62 non-redundant targets were selected through the TCMSP database and analysis platform. The target genes underwent gene ontology and pathway enrichment analysis. The gene list applied to the gene ontology analysis revealed associations with various biological processes, including signal transduction, chemical synaptic transmission, G-protein-coupled receptor signaling pathways, response to xenobiotic stimulus, and response to drugs, among others. A total of eleven genes, including HSP90AB1, CALM1, F2, AR, PAKACA, PTGS2, NOS2, RXRA, ESR1, ESR2, and NCOA1, were found to be associated with biological pathways related to cancer metastasis. Furthermore, nineteen of the active compounds from Dichroae Radix were confirmed to interact with these genes. Conclusions : The results provide valuable insights into the mechanism of action and molecular targets of Dichroae Radix. Notably, Berberine, the main active ingredient of Dichroae Radix, plays a significant role in degrading AR proteins in advanced prostate cancer. Further studies and validations can provide crucial data to advance cancer metastasis prevention and treatment strategies.

• 대한한의학방제학회지
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• 제32권3호
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• pp.247-261
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• 2024

Objectives : Zuojin Pill, recognized as an effective herbal remedy, has undergone investigation for its potential in alleviating symptoms like indigestion, vomiting, and abdominal distension. The purpose of this study was to investigate the mechanism of digestive function activation through network pharmacology, particularly focused on improving functional dyspepsia. Methods : The two components, Coptidis Rhizoma and Evodiae Fructus, constituting Zuojin Pill were analyzed based on broad information on chemical and pharmacological properties, confirming 40 active compounds and 115 digestive-related molecular targets. Concentration analysis revealed impacts on various pathways related to digestive functions. Results : According to network pharmacological analysis of Zuojin Pill, quercetin and beta-sitosterol were exhibited relatively numerous targets, suggesting their potential significance in the therapeutic activity of Zuojin Pill and by a Protein-Protein Interaction (PPI) network, JUN, RELA, MAPK1, HSP90AA1, TP53, TNF, AKT1, IL6, MAPK14, ESR1, FOS, MYC were identified. Also, berberine exhibited the highest contribution index (92.58%), indicating that this compound may be a major contributor to the digestive activity of Zuojin Pill. Additionally, functional interaction analysis by GeneMANIA indicated that targets of Zuojin Pill could functionally interact through various mechanisms, implying similarities in pharmacological roles. Conclusions : These findings contribute valuable insights into the digestive function activation mechanism and highlight the therapeutic potential of Zuojin Pill in improving functional dyspepsia.

• 한국식품과학회지
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• 제35권6호
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• pp.1098-1103
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• 2003

열처리에 의한 대두 단백질의 용출 변화를 조사하기 위하여 만리, 태광, 은하, 풍산나물, 검정콩 1호와 일품검정의 6가지 장려품종 대두를 온도와 시간에 따라 열처리를 달리하여 침치액의 pH와 가용성 고형물함량과 단백질 특성을 살펴보았다. $60^{\circ}C$에서 90분 동안 열처리 후 침지액의 pH는 열처리 전보다 높았으며 $60^{\circ}C$에서 열처리 시간이 증가함에 따라 pH는 감소하였다. 침지액의 가용성고형물 함량은 열처리 온도에 따라서 $50^{\circ}C$ 이후 급격한 증가를 보였으며, 열처리 시간에 따라서 용출량의 증가를 보였다. 다른 대두에 비해 풍산나물과 은하의 경우 시간에 따라 비례적인 증가경향을 보였으며 검정콩 1호와 일품검정은 낮은 당도를 나타내었다. 단백질함량은 열처리 온도와 시간이 증가함에 따라 증가하였는데 검정콩1호와 일품검정은 다른 대두에 비해 각각 2배와 4배 정도 높은 단백질 농도를 나타내었다. 검정콩1호, 만리 및 은하 품종의 SDS-PAGE 특성은 모든 대두에서 비슷한 분리대를 보였다. 열처리 온도가 증가함에 따라 전체적으로 단백질 용출량이 증가하였고, $50^{\circ}C$ 및 $60^{\circ}C$에서 대략 31kDa과 16kDa의 새로운 단백질이 뚜렷하게 증가하였다. 열처리 시간이 증가함에 따라서도 단백질 용출량은 증가하였고 새로운 단백질 분획은 다른 단백질 보다 용출량이 더 증가하였다. 선행연구와의 비교로 열처리에 의해 나타난 31kDa와 16kDa의 단백질은 장려품종 대두에서 합성된 열충격 단백질(heat shock protein, HSP)이 용출된 것으로 판단하였다.養素) 이용율중(利用率中) 지방이용율은 대체로 일반식이 내의 륵지방(肋脂肪)보다는 지방질(脂肪質) 첨가식이내(添加食餌內)의 륵지방(肋脂肪)이 흡수율(吸收率)이나 이용률(利用率)이 더 우수함을 보여주었고, 근소축적률(筋素蓄積率)은 쥐에 있어서는 어유(魚油)군이 73.5%, 병아리에 있어서는 채종유군이 52.1%로 가장 높았으며 또한 쥐에서는 참기름군이 66.0%, 병아리에서는 대조구가 33.4로 가장 낮았으나 각 구별 통계적(統計的)인 차이는 없었다. 4. 쥐 실험(實驗)에서만 실시된 간지방(肝脂防) 함량측정(含量測定)은 채종유군의 그것이 다른 군보다 높았으며 옥수수기름, 콩기름, 참기름, 들기름, 동물유, 어유의 순서였으나 통계적(統計的)인 유의성(留意性)은 인정(認定)되지 않았고 일반적(一般的)으로 식물성 유지급여군의 간지방함량(肝脂肪含量)이 다른 군보다 높았다. 5. 혈청(血淸)콜레스테롤 함량(含量)은 쥐에 있어서는 채종유군이 가장 높았으며 참기름군이 비교적(比較的) 높은 수치(數値)를 나타냈고, 병아리실험에서는 동물유군이 어유, 콩기름, 참기름, 들기름, 옥수수기름, 채종유, 대조군보다 높았으나 통계적(統計的)인 유의차(留意差)는 없었다.6. 지방산조성(脂肪酸造成)은 동물성유지는 대체로 palmitic acid, myristic acid함량이 많았으며 식물성유는 Linoleic acid 와 oleic acid가 많았고 옥수수기름이 필수지방산인 Linoleic acid 함량이 54.7%로 가장많았으며 특히 들기름이 2중 결합 3개인 Linolenic acid가 58.4%로 다른 식물성기름보다 월등히 높았다. 한편 식물성 기름에는 Arachidonic acid 가 소량있으나 동물유(動物油)나 어유(魚油)에서는 분석(分析)되지 못했다.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2005년도 2005 Annual Meeting & International Symposium
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• pp.154-164
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• 2005

Saccharomyces cerevisiae KNU5377 is a thermotolerant strain, which can ferment ethanol from wasted papers and starch at 40$^{\circ}C$ with the almost same rate as at 30$^{\circ}C$. This strain showed alcohol fermentation ability to convert wasted papers 200 g (w/v) to ethanol 8.4% (v/v) at 40$^{\circ}C$, meaning that 8.4% ethanol is acceptable enough to ferment in the industrial economy. As well, all kinds of starch that are using in the industry were converted into ethanol at 40$^{\circ}C$ with the almost same rate as at 30$^{\circ}C$. Hyperthermic cell killing kinetics and differential scanning calorimetry (DSC) revealed that exponentially growing cells of this yeast strain KNU5377 were more thermotolerant than those of S. cerevisiae ATCC24858 used as a control. This intrinsic thermotolernace did not result from the stability of entire cellular components but possibly from that of a particular target. Heat shock induced similar results in whole cell DSC profiles of both strains and the accumulation of trehalose in the cells of both strains, but the trehalose contents in the strain KNU5377 were 2.6 fold higher than that in the control strain. On the contrary to the trehalose level, the neutral trehalase activity in the KNU5377 cells was not changed after the heat shock. This result made a conclusion that though the trehalose may stabilize cellular components, the surplus of trehalose in KNU5377 strain was not essential for stabilization of whole cellular components. A constitutively thermotolerant yeast, S. cerevisiae KNU5377, was compared with a relatively thermosensitive control, S. cerevisiae ATCC24858, by assaying the fluidity and proton ATPase on the plasma membrane. Anisotropic values (r) of both strains were slightly increased by elevating the incubation temperatures from 25$^{\circ}C$ to 37$^{\circ}C$ when they were aerobically cultured for 12 hours in the YPD media, implying the membrane fluidity was decreased. While the temperature was elevated up to 40$^{\circ}C$, the fluidity was not changed in the KNU5377 cell, but rather increased in the control. This result implies that the plasma membrane of the KNU5377 cell can be characterized into the more stabilized state than control. Besides, heat shock decreased the fluidity in the control strain, but not in the KNU5377 strain. This means also there's a stabilization of the plasma membrane in the KNU5377 cell. Furthermore, the proton ATPase assay indicated the KNU5377 cell kept a relatively more stabilized glucose metabolism at high temperature than the control cell. Therefore, the results were concluded that the stabilization of plasma membrane and growth at high temperature for the KNU5377 cell. Genome wide transcription analysis showed that the heat shock responses were very complex and combinatory in the KNU5377 cell. Induced by the heat shock, a number of genes were related with the ubiquitin mediated proteolysis, metallothionein (prevent ROS production from copper), hsp27 (88-fold induced remarkably, preventing the protein aggregation and denaturation), oxidative stress response (to remove the hydrogen peroxide), and etc.

• Reproductive and Developmental Biology
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• 제37권3호
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• pp.149-154
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• 2013

The aim of this study was to evaluate the changes of protein patterns in granulosa cells and corpus luteum in ovaries during the estrus cycle in cows. The estrus cycle was devided into five steps of follicular, ovulatory, early-luteal, mid-luteal and late-luteal phases. In results, 61 spots of total 85 spots were repeated on follicular phase and 51 spots of total 114 spots were repeated on ovulatory phase. The 40 spots of total 129 spots were repeated on early-luteal phase and 49 spots of total 104 spots were repeated on mid-luteal phase. Also 41 spots of total 60 spots were repeated on late-luteal phase. On the other hands, the 16 spots were indicated difference in follicular phase and ovulation phase had a difference 10 spots. It was showed difference No. 103 spot in ovulation phase, No. 135 spot in early-luteal phase and No. 175 and 176 spots in mid-luteal phase. Also, the 11 spots were expressed specifically in mid-luteal phase and No. 178 and 179 spots were difference of expression in late-luteal phase. We confirmed that there were 7 spots for ovulation, 4 spots for luteinization and 2 spots for luteolysis. Spot No. 89~93 in ovulation phase were transferrin, and spot No.94~98 were HSP60. Spot No. 103 was Dusty PK, spot No. 135 was OGDC-E2, and spot No. 175 and 176 were Rab GDI beta from luteinization. Spot No. 178 and 179 in luteolysis were vimentin. This results suggest that will be help to basic data about infertility.

• Biomolecules & Therapeutics
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• 제16권3호
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• pp.197-202
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• 2008

In our previous publication we compared the gene expression profiles on hepatotoxicants exposure to assess the comparability between in vivo and in vitro test systems. We investigated global gene expression from both mouse liver and mouse hepatic cell line treated with thioacetamide (TAA) and identified several common genes. In this study, we selected genes to validate them as potential biomarkers for hepatotoxicity on the relevance of in vitro and in vivo system. Three up-regulated, aquaporin 8 (Aqp8), glutathione peroxidase 1 (Gpx1), succinate-CoA ligase, GDP-forming, alpha subunit (Suclg1) and two down-regulated, DnaJ (Hsp40) homolog subfamily C member 5 (Dnajc5) and tumor protein D52 (Tpd52) genes were tested for their effects in vitro. For characterization of gene function, short interfering RNA (siRNA) for each gene was synthesized and transfected in mouse hepatic cell line, BNL CL.2. Cell viability, mRNA expression level and morphological alterations were investigated. We confirmed siRNA transfection against selected five genes induced down-regulation of respective mRNA expression. siRNA transfection in general decreased cell viability in different degrees and induced morphological changes such as membrane thickening and alterations of intracellular structures. This suggests that these genes could be associated with TAA-induced toxicity. Furthermore, these genes may be used in the investigation of hepatotoxicity for better understanding of its mechanism.