• Natural Product Sciences
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• 제13권3호
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• pp.214-219
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• 2007

The roots of Pseudarthria viscida (L.) Wight and Arnott. (Leguminosae) are used against various disorders in Indian systems of medicine, namely as anthelmintic, anti-inflammatory, cardiotonic, aphrodisiac, rejuvenating, tonic and as a remedy for cough, asthma, tuberculosis, diarrhoea and alternate fever. The present communication deals with the detailed pharmacognostical evaluation of the root sample using light and confocal microscopy, WHO recommended physico-chemical determinations and authentic phytochemical procedures. he physico-chemical, morphological and histological parameters presented in this paper may be proposed as parameters to establish the authenticity of P. viscida root and may possibly help to differentiate the drug from its other species.

• 한국염색가공학회지
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• 제10권6호
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• pp.27-32
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• 1998

도시 Traditionally, dyes extracted from onion shells have been used as natural pigments but dyeing methods with reproducibility have not been developed. In this study, the dyes were analyzed by high performance thin layer chromatography(HPTLC) to obtain the ratio of pigments in water and ethyl acetate extract. Furthermore, the present study was also carried out to obtain the information characteristic of wavelength which proportionally decrease for continuous dyeing reaction. Scanning of water extract showed 4 peaks by the mobile phase of benzene/ethyl acetate/acetic acid(40/10/5) and the peak 1 exhibited yellow color with the maximal absorption spectra of 306nm and 309nm while ethyl acetate extract showed 9 peaks in the same mobile phase. The water extract after ethyl acetate extraction was adjusted to 0.5 of O.D.(optical density) at 550nm by adding of water and compared wavelength of the pigments from 200 to 600nm to find the proportional decrease of wavelength. As the result, it showed that wavelength of 306nm could be the standard of dyeing monitoring points.

• BMB Reports
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• 제28권5호
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• pp.427-432
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• 1995

Ganglioside GM3 and sialidase activities in human fetal liver have been investigated. Gangliosides were extracted from fetal livers by the Folch-Suzuki method and analyzed by high-performance thin layer chromatography (HPTLC). GM3 increased, but lactosylceramide (LacCer) decreased predominantly over the developmental stages. Sialidase in human fetal liver was mainly localized in the lysosomal fraction and its activity was high in the earlier stages of development. The optimum pH for this enzyme was 4.3~4.4. Sialidase was more active with the ganglioside mixture than with GM3, sialyllactose or fetuin. Fetal liver sialidase was still active (20% activity) in the presence of 25% methanol. These results suggested that the changes of the ganglioside GM3 and sialidase activity may be involved in the regulation of cell growth in human fetal liver during development.

• 한국미생물·생명공학회지
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• 제21권6호
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• pp.556-563
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• 1993

AF-011A is a novel lipopeptide with potent antifungal activity isolated from Pseudomonas cepacia AF6008 deposited as KFCC 10759. The compound was isolated from the fermentation broth by extraction with 50% isopropyl alcohol. Purification was effected by chromatography on Diaion HP-20, Alumina and C18 followed by HPTLC on silica gel. These techniques affored two closelt related compounds. AF-011A1 ans AF-011A2. The molecular weights of AF-011A1/A2 were determined by fast atom bombardment mass spectrometry(A1 m/z 1,215 : A2 m/z 1,199).

• Advances in Traditional Medicine
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• 제10권2호
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• pp.123-133
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• 2010

The entire plant of Leptadenia reticulata (Asclepidaceae) is extensively used as lactogen, traditionally, in veterinary practice. The plants of Dregea volubilis and Pentatropis microphylla (Asclepidaceae) are now used as its substitute and sometimes replace the original drug as traditional lactogen. The lactogenic potential of these drugs was studied in rats using, pup weight, weight of mother, parenchyma percentage, secretary rating, estimation of total protein content and glycogen content of mammary glands tissues as assessment parameters. HPTLC profiles of bioactive extracts were also generated to serve the authentification needs. The results of present studies show that P.microphylla forms a better substitute over D. volubilis.

• Journal of Ginseng Research
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• 제22권2호
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• pp.91-95
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• 1998

The distribution of phenolic components of Korean ginseng (Panax ginseng KG) and American ginseng (Panax quinquefolium, AG) were compared by thin-layer chromatography (TLC). Silica gel TLC gave 3~4 spots, while $NH_2$ HPTLC 5~6 spots, which were colored by both $FeCl_3$/$K_3Fe(CN)_6$ and Folin-Ciocalteu. The distribution of phenolic components was quite different between KG and AG. Especially, a polyphenol (m.w. 578), which had been isolated from KG by the author, was not found in AG. This result suggests that the polyphenol could be used as an index compound for the differentiation of KG from AG.

• Advances in Traditional Medicine
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• 제10권3호
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• pp.184-190
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• 2010

Berberis lycium (family Berberidaceae) has a close affinity with B. aristata, used in India Traditional Systems of Medicine as a drug 'Daruharidra' for skin disease, jaundice, affection of eyes, and rheumatism. Various species of Berberis are being sold in India herbal drug market. During the market surveillance of different herbal drug markets of India, it was observed that almost all the markets either comprise of Berberis lycium or Berberis asiatica. Keeping this in view, in the present study attempts have been made to identify marker characters of this potent species.

• Natural Product Sciences
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• 제14권4호
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• pp.221-226
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• 2008

The bark of Careya arborea Roxb. (Barringtoniacae) is used against various disorders in Indian systems of medicine, namely astringent, anthelmintic, in tumors, dyspepsia, colic, bronchitis, urinary discharges, piles, lecucoderma, skin diseases, epileptic fits, diarrhoea. The juice of the fresh bark with honey is given as a demulcent in coughs and colds, infusion of the fresh bark is used to treat snake bite by applying to the bitten part and also taken internally. The present communication deals with the detailed pharmacognostical evaluation of the bark sample using light and confocal microscopy, WHO recommended physico-chemical determinations and authentic phytochemical procedures. The physico-chemical, morphological and histological parameters presented in this paper may be proposed as parameters to establish the authenticity of C. arborea bark and may possibly help to differentiate the drug from its other allied species.

• 한국식품과학회지
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• 제34권4호
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• pp.546-551
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• 2002

국내에서 채집되는 propolis에 대한 기초자료를 얻고자 아까시나무와 밤나무를 밀원 식물로 하여 채집된 propolis와 그의 70% ethanol 추출물(EEP)에 대한 영양성분 및 페놀성 화합물을 분석하였다. 일반성분은 밀원식물에 따른 큰 차이 없이 비슷한 분포를 보였고, EEP의 경우 propolis 분말에서보다 대부분의 성분들이 상당량 감소되었다. 주요 무기질 5종은 일반성분과는 달리 채집지역과 수종에 따른 차이를 보였고, 대체로 아까시나무의 propolis가 무기물 함량이 많았다. 아까시나무의 propolis에는 칼슘함량이 가장 많았고, 밤나무 propolis는 칼륨이 가장 많이 함유되어 있었다. 반면에 EEP에서는 두 시료 모두 칼륨의 함량이 가장 많았다. 아미노산은 총 16종이 검출되었고, aparagine, cysteine, glutamine 및 tryptophan은 검출되지 않았다. 특히 밤나무 propolis에서는 methionine도 검출되지 않았다. Aspartic acid가 가장 많았고, methionine의 함유량은 극히 적었으며, 아까시나무 propolis가 아미노산 함유량이 전반적으로 더 높았다. 추출수율이 비교적 높게 나타난 EEP의 total polyphenol 함량은 $13.9{\sim}23.7%$였고, propolis의 phenolic fraction중 대부분을 차지하는 성분인 flavonoids 함량은 $8.6{\sim}10.8%$로 나타나 다른 식품재료에 비하여 매우 높은 수준이었다. 고성능 박막크로마토그래피로 페놀성 화합물을 정성분석한 결과 시료종류에 상관없이 거의 동일한 pattern의 chromatogram을 나타내었다. Polyphenol 화합물의 HPLC 분석에서는 약 16개의 peak가 검출되었고, 이중 p-hydroxy benzoic acid, caffeic acid, ferulic acid, benzoic acid, cinnamic acid 및 chrysin의 6종을 동정하였다. 시료간의 chromatographic pattern은 동일하여 propolis를 구성하는 페놀성 화합물의 종류는 큰 차이가 없는 것으로 판단되었다.

• Natural Product Sciences
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• 제11권3호
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• pp.165-169
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• 2005

Chlorophytum borivilianum Baker (Antharicaceae) commonly referred as 'Safed Musli' has been widely used in the Indian traditional systems of medicine to treat various diseases like rheumatism apart from having immunomodulating property and is used as general tonic. It is also known as 'Ayurvedic viagra' for its aphordisiac properties. C. borivilianum was screened for the first time to determine its antioxidant activity, isolation of the sapogenins and standardization of the isolated sapogenin fraction using HPTLC. Potent antioxidant activity of ethanolic extract was found by their ability to scavenge DPPH (84.51%), hydroxyl radical (48.95 %), ferryl bi-pyridyl complex (84.53%) along with the inhibition of lipid peroxidation (67.17%) at $100\;{\mu}g/ml$ concentration. The ethanolic extract also exhibited significant inhibition of superoxide anion radical generated by photochemiluminescence. Thus, the potent antioxidant activity validates the innumerable therapeutic claims of the plant in the traditional system especially its use as a Rasayana drug.