• 한국식품영양과학회지
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• 제22권5호
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• pp.581-585
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• 1993

참죽나무(Cedrela sinensis A. Juss.) 잎의 ethyl acetate분획으로부터 분리한 5종의 flavonoid성분들은 HPLC에 의한 용매계 THF-dioxane-MeOH-HOAc-5% $H_3PO_4-H_2O$(145 : 125 : 50 : 20 : 2 : 658)에서 양호한 분리능을 나타내었다. 또한 주성분인 quercitrin의 함량은 MeOH엑스 및 EtOAc 분획에서 각각 9.48%(w/w) 및 37.06%(w/w) 함유되어 있음을 알 수 있었다.

• 생약학회지
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• 제23권4호
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• pp.229-233
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• 1992

From the leaves of Ulmus parviflora isoquercitrin and rutin were isolated and identified by chemical and spectral analysis. The content of rutin was determined by HPLC, and it was about 2.81% in methanol extract.

• Journal of Plant Biology
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• 제36권3호
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• pp.297-300
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• 1993

Six flavonoid compounds from the ethyl acetate fraction of Machilus thunbergii leaves were identified by HPLC. Separation by reversed phase chromatography on u-Bondapak C18 column was achieved by isocratic elution. The contents of the major flavonoids, guaijaverin and quercitrin were about 0.48% (w/w) and 0.98% (w/w) for the methanol extract, respectively.

• 생약학회지
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• 제25권3호
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• pp.214-220
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• 1994

From the Viticis Fructus n-hydrocarbons, ${\beta}-sitosterol$ $3-O-{\beta}-_D-glucoside$ and hesperidin along with the known polyoxygenated flavonoids such as vitexicarpin, artemetin and luteolin, and vanillic acid were isolated and identified by means of spectroscopic methods. HPLC analysis of the flavonoid components from the MeOH extract was established. Phytochemical analyses of the domestic plant sample and the imported ones were conducted and the flavonoid compositions of the domestic samples were greatly different from those of the imported ones.

• Natural Product Sciences
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• 제15권4호
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• pp.208-212
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• 2009

The heartwood of Rhus verniciflua Stokes (Anacardiaceae) is widely used for a medicinal plant to treat diabetes mellitus and lingering intoxication in the folkloric society of Korea, while the stem bark has been traditionally used to treat menstrual disorder and helminthiasis. We previously reported that a flavonoid, fustin, isolated from the heartwood of R. verniciflua is effective in Alzheimer‘s disease and rheumatoid arthritis. To explore the possibility to produce more flavonoid-rich fraction from this plant, the MeOH extracts from the plant parts of stem bark, outer heartwood, and inner heartwood were quantitatively analyzed by HPLC. Phenolic or flavonoid compounds (chlorogenic acid, caffeic acid, p-coumaric acid, sulfuretin, fustin, fisetin, luteolin and astragalin) were detectable in the HPLC chromatogram. The orange-colored inner heartwood was found to contain the highest levels of fustin (16.96 mg/g) and sulfuretin (2.22 mg/g). Moreover, the inner heartwood accumulated fustin and sulfuretin at least 4-fold higher level as compared to the stem bark and outer heartwood. The levels of total phenolic compounds positively correlated with the extents of antioxidant properties. Therefore, the inner heartwood of R. verniciflua could be used to increase fustin concentration of the extract which is capable of treating Alzheimer‘s disease and rheumatoid arthritis.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2009년도 심포지엄 및 춘계학술발표회
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• pp.227-228
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• 2009

• Journal of Applied Biological Chemistry
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• 제64권3호
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• pp.269-275
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• 2021

The in vitro anti-obesity activity of 12 species of tree sprouts in differentiated 3T3-L1 cells and the mechanisms underlying their activity were evaluated. (+)-Catechin and quercetin concentrations in the sprouts were analyzed by HPLC/UV at 270 and 254 nm, respectively. Euonymus alatus (EAT) and Fraxinus mandschuria (FMS) extracts at doses of 50 and 100 ㎍/mL inhibited the accumulation of lipid droplets in differentiated 3T3-L1 cells. Moreover, EAT and FMS downregulated the expression of the CCAAT/enhancer-binding protein-α, adipogenesis-related proteins peroxisome proliferator-activated receptor-γ, and adipocyte P-2α in differentiated 3T3-L1 cells. Tree sprouts with an abundant flavonoid content exerted the highest anti-obesity activity. Concentrations of total flavonoids were the highest in FMS (24.281 mg/g DW) sprouts. These findings could be used to develop health-promoting functional foods or supplements derived from tree sprouts.

• 한국식품영양과학회지
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• 제24권6호
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• pp.901-905
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• 1995

기호음료 및 약용으로 사용되는 두충나무잎의 품질 평가를 위한 HPLC로서 함유된 flavonoid 화합물을 정성, 정량 분석하였다. 즉 두충나무잎의 n-BuOH 분획으로 부터 astragalin, isoquercitrin, quercetin $3-O-{\beta}-D-xylopyranosyl(1-2)-{\beta}-D-glucopyranoside$의 3종 flavonoid 성분들을 분리하였으며, 이들 화합물은 LPLC의 THF-dioxane-MeOH-HOAc-5% $H_3PO_4-H_2O$(145 : 125 : 50 : 20 : 2 : 658) 혼합용매에서 양호한 분리능을 나타내었다. MeOH 엑스와 n-BuOH 분획 중에 astragaline의 함량은 각각 0.09%(w/w), 0.46%(w/w), isoquercitrin은 0.08%(w/w), 0.48(w/w), quercetin $3-O-{\beta}-D-xylopyranosyl(1-2)-{\beta}-D-glucopyranoside$은 0.40%(w/w), 1.22%(w/w) 함유되어있다.

• Natural Product Sciences
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• 제27권4호
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• pp.284-292
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• 2021

Recently, a phytoestrogenic functional food has been developed using the fruits of Sophora japonica. Phytochemical investigation of fruits of S. japonica led to the isolation of eight flavonoid glycosides using various chromatographic techniques. The isolated compounds were identified as genistin (1), sophoricoside (2), genistein 7,4'-di-O-β-D-glucopyransoide (3), sophorabioside (4), genistein-7-O-β-D-glucopyranoside-4'-O-[(α-L-rhamnopyranosyl)-(1→2)-β-D-glucopyranoside] (5), sophoraflavonoloside (6), nicotiflorin (7) and kaempferol-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranosyl-(1→3)-β-D-glucopyranoside (8), respectively, by comparison of their spectroscopic data with those reported in the literature. In addition, a new HPLC-DAD method for simultaneous determination of the isolated compounds was developed to quantitate the contents of flavonoids in S. japonica and S. flavescens. The method was validated in terms of limit of detection, limit of quantitation, specificity, linearity, precision and accuracy. The validated method was successfully applied to determine eight flavonoids in two Sophora species. The contents of eight flavonoids varied according to the parts and species. Particularly, it was found that only the fruits of S. japonica contained sophoricoside, a phytoestrogenic isoflavone.

• Natural Product Sciences
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• 제22권1호
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• pp.1-12
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• 2016

Compositional differences in flavonoids are varied in the big family of Compositae. By summarizing our previous analytical studies and other scientific evidences, new strategy will be possible to further analyze flavonoids and utilize them for human health. The HPLC analytical method has been established in terms of linearity, sensitivity, accuracy, and precision. Herbs of the family of Compositae have considerable amounts of peroxynitrite ($ONOO^-$)-scavenging effects and their phenolic substances. These effects may contribute to the prevention of disease associated with excess production of $ONOO^-$, depending on the high content of flavonoid substances.