• 한국환경과학회지
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• 제11권5호
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• pp.455-461
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• 2002

Polycyclic aromatic hydrocarbons(PAHs) are ubiquitous contaminants in coastal marine environment. PAHs enter estuarine and nearshore marine environment via several routes such as combustion of fossil fuels, domestic and industrial effluents and oil spills. In August of 1997, mussels(Mytilus edulis) were collected at 6 sites near Kori nuclear power plant in order to analyze the PAH content by HPLC with uv/vis detection. Unfortunately, I could not find any living oysters in which I firstly intended to measure the PAH content in the study area. NPTHL and ANCPL were the major dominant PAH compounds in mussels living in the intertidal zone of Kori, Korea, and DahA, BbF, BaP were the next dominant PAH group in mussels in the study area. The mean concentrations of 15 PAH in mussels ranged from 3.2 to 1,680 ppb(mean 105$\pm$60.5 ppb). Compared with other studies world over, the concentrations of carcinogenic PAHs were relatively low in mussels in the study area, even though total PAH content was rather high. According to N/P(Naphthalene/Phenanthrene) ratio(147) and the ratio of 2∼3 ring to 3∼5 ring PAHs(58∼90 %) in mussels in the study area, I expect that the major source of PAHs in this study area is rather fresh petroleum-derived. This study presents preliminary data for the PAH levels in mussels from the intertidal zone of Kori, and the data will hopefully be utilized for the assessment of oil pollution in the East Sea, Korea.

• Archives of Pharmacal Research
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• 제29권1호
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• pp.96-101
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• 2006

Phytoestrogens have been used as a food supplement to prevent osteoporosis. The isoflavones in the phytoestrogens are daidzein, genistein and formononetin which are present in various herbs. This study examined the quantity of isoflavones in medicinal herbs, which can be used as a phytoestrogen supplement; soybean. These isoflavones were quantified using high performance liquid chromatography (HPLC) with a UV/VIS detector. The concentration of daidzein in Puerariae Radix was $10,436.16{\pm}2,143.83\;mg/kg$ of the dried herb, which was much higher than that extracted from soybeans, $341.47{\pm}18.96\;mg/kg$. The amount of genistein in Sophorae flavescentis Radix ($336.09{\pm}50.89mg/kg$) was approximately 11 times higher than that extracted from soybean ($30.03{\pm}7.17mg/kg$). The level of formononetin in Dalbergiae odoriferae Lignum, $2,189.14{\pm}136.46mg/kg$, was the highest among the herbs tested. The total isoflavone content of Puerariae Radix was approximately 30 times higher than that extracted from soybean. Therefore, plants from the family Leguminosae, particularly Puerariae Radix, can be a good source of phytoestrogens.

• 한국응용과학기술학회지
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• 제14권1호
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• pp.103-107
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• 1997

This study was carried out to separate and identify the antioxidative substances in persimmon leaves. The antioxidative substances in persimmon leaves were extracted by methanol. The extract was fractionnated by SEP-PAK cartridge colum. From these results five fractions(F-I${\sim}$V) were obtained. Antioxidative activity of each fractions was examined by the DPPH methord. The F-II, III and IV showed antioxidative activity and among them F-II and F-III showed the strongest. Five frations were separated by TLC using ethylacetate : chloroform : formic acid : $H_2O$(8 : 1 : 1 : 1 v /v) as the solvent. From these results were obtained spots of Rf 0.71, 0.35 and 0.25. This spots were scraped from the plate and extracted by methanol. The extracts thuse obtained were used for examination of identify by TLC, UV /VIS-spectrophotometer and HPLC. Among them spot of Rf 0.71 were demonstrated to catechin and the spots of Rf 0.35 and 0.25 was suggested to polyphenol substances.

• 한국대기환경학회지
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• 제11권4호
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• pp.339-349
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• 1995

The purpose on this study is to optimize the chromatographic determination method of trace aldehydes in ambient air. Carbonyl compounds in urban air were trapped at $C_{18}$ DNPH-coated cartridges, and generated hydrazone derivatives were separated by HPLC and detected by UV-vis spectroscopic detector at 360nm. Formaldehyde and acetaldehyde compounds could be isolated from urban(Seoul) air with more than 95% collection efficiency. The analytical detection limits for formaldehyde and acetaldehyde are 0.06pp $b_{v}$, 0.08pp $b_{v}$ for 108 L air samples, respectively. The precision of this method are 3 .sim. 4%(RSD) for mutiple injection of hydrazone standards. Separation of seven dinitrophenylhydrazones could be achieved in appoximately 20 minutes operation time using $C_{18}$ column with apprepriate eluent. The method was applied to the analysis of aldehydes and ketone in Seoul ambient air. The 24-h ambient levels of formaldehyde, acetaldehyde reached up to 6 .sim. 14 and 3 .sim. 8ppbv, respectively. The daily average concentration ratio were 0.60 for acetaldehyde/formaldehyde.yde.

• Journal of Applied Biological Chemistry
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• 제63권3호
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• pp.197-201
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• 2020

In order to enhance the extraction efficiency of flavonoid from Saururus chinensis, carbohydrate-hydrolyzing enzyme Viscozyme L aided extraction techniques have been studied. Then flavonoid composition, as well as quercetin, were also identified using UV/Vis, HPLC/MS, and ¹H-NMR. The results showed that favorable extraction conditions were Viscozyme L concentration of 0.25 mg/g, pH 4.2, reaction at 45 ℃ for 12 h. Under the favorable extraction condition, total flavonoid yield (37.9 mg/g) and quercetin yield (0.86 mg/g) increased by about 2.0 and 9.6 times, respectively, compared to control group. Interestingly, as a significant flavonoid of S. chinensis, flavonoid glycones rutin was hydrolyzed to aglycones quercetin by Viscozyme L. These findings provide scientific and theoretical support for the development quercetin-rich products, which was quickly absorbed by the human body than rutin.

• 한국재료학회지
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• 제33권11호
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• pp.458-464
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• 2023

In order to develop catechin patches for skin regeneration at wound sites, patches with varying concentrations of catechin and chitosan were manufactured. An optimal composition ratio was determined by adjusting the drug release rate and amount, to maximize efficiency. The catechin used in this study was extracted from green tea leaves using a solvent/ultrasonication method, and its characteristics were confirmed through Fourier transform-infrared spectroscopy (FT-IR) and high-performance liquid chromatography (HPLC) analyses. Patches were prepared with different concentrations of catechin and chitosan, and various properties were analyzed using techniques such as FT-IR, water contact angle analysis, and UV-Vis spectroscopy. It was observed that as the chitosan concentration increased, the release of catechin slowed down or almost ceased. A patch manufactured with 1.5 mg/cm² of catechin at a 1 % chitosan concentration exhibited a high initial release rate over 24 h and demonstrated cellular biocompatibility. Consequently, these patches, with tailored release characteristics based on the concentrations of chitosan and catechin, hold promise for use as drug delivery systems in wound healing applications.

• Korean Chemical Engineering Research
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• 제61권4호
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• pp.635-644
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• 2023

Due to the rapid development of the livestock industry, particularly due to residual pharmaceutical antibiotics, environmental populations have been negatively affected. Herein, we report a ZnO/melamine-functionalized carboxylic-rich graphene oxide (ZFG) photocatalyst for visible light-driven photocatalytic degradation of tetracycline hydrochloride in aqueous solutions. The properties of the photocatalysts were evaluated by XRD, FTIR, XPS, Fe-SEM, HR-TEM, TGA, Raman spectroscopy, UV-Vis spectroscopy, zeta potential, and electrochemical measurements. The photocatalytic activity was measured using high-performance liquid chromatography. The photocatalytic properties of the ZFG photocatalyst evaluated against the tetracycline hydrochloride (TCH) antibiotic under visible light irradiation showed superior photodegradation of 96.27% within 60 min at an initial pH of 11. The enhancement of photocatalytic degradation was due to the introduction of functionalized graphene, which increases the light-harvesting capability and molecular adsorption capability in addition to minimizing the recombination rate of photogenerated charge carriers due to its role as an electron acceptor and mediator.

• International Journal of Oral Biology
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• 제36권2호
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• pp.59-64
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• 2011

Anthocyanins are naturally occuring phytochemicals and the main components of the coloring of plants, flowers and fruits. They are known to elicit antioxidative, anti-inflammatory and cancer preventive activity. In this study, we investigated anthocyanins in black / yellow soybean seedcoats using different methods of detection - thin layer chromatography (TLC), capillary zone electrophoresis (CZE) and HPLC analysis. The anthocyanins in soybean seedcoats were extracted by five independent methods of extraction and the aglycons (anthocyanidins) of the corresponding anthocyanins were prepared by acid mediated hydrolysis. The anthocyanin / anthocyanidin in black soybean seedcoat showed characteristic TLC mobility, CZE electrophoretic retention and HPLC migration time while little of anthocyanins were detected from yellow soybean seedcoat. The extracted anthocyanins showed pH dependent retention time in CZE and spectral change in UV-Vis spectrum. HPLC analysis of the hydrolyzed extract of black soybean seedcoat identified the presence of four anthocyanidins. The major anthocyanin in black soybean seedcoat was cyanin (cyanidin-3-O-glucoside), with the relative order of anthocyanidin in cyanidin > delphinidin > petunidin > pelargonidin.

• 한국작물학회지
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• 제53권4호
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• pp.359-368
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• 2008

검정콩 품질평가의 한 기준이 되는 안토시아닌의 함량평가를 위한 분석기술 개발의 일환으로 검정콩 종피 함유 안토시아닌의 최적 HPLC 분석조건을 확립하여 검정콩 함유 안토시아닌의 작물학적 연구와 검정콩 함유 안토시아닌의 이용도 증진 및 상업적 활용에 기초 자료를 제공하고자, 검정콩에 함유된 3종의 안토시아닌 성분을 동시에 분석할 수 있고, 재현성 및 검출 감도가 증진된 최적의 HPLC 분석 조건을 검토한 결과를 요약하면 다음과 같다. 1. 검정콩에 함유된 3종의 안토시아닌 D3G, C3G 및 Pt3G를 동시에 분석할 수 있는 최적의 HPLC 분석 조건을 검토한 결과 컬럼은 YMC-pak ODS-AM 303($4.6{\times}250\;mm$, $5\;{\mu}m$) 컬럼을 이용하고, 검출파장은 520 nm, 컬럼온도를 $30^{\circ}C$, 분당유속은 0.7 mL, 이동상 용매로는 A용매로 5% 포믹산 - 증류수, B용매로 5% 포믹산 - 아세토니트릴을 사용하여 농도구배 조건으로 분석하는 것이 가장 분리능이 향상된 효율적 분석법으로 조사되었다. 2. 검정콩에 함유된 3종의 안토시아닌 D3G, C3G 및 Pt3G를 동시에 분석할 수 있는 최적 HPLC 분석 조건의 재현성 검정을 실시한 결과 머무름시간(Rt.)의 변이계수는 최대 1.24%, peak height의 변이계수는 최대 2.04%, peak area의 변이계수는 최대 0.21% 미만을 나타내므로 확립된 검정콩 함유 안토시아닌 동시분석을 위한 최적 HPLC 분석 조건은 극히 높은 분석 재현성을 나타내었다. 3. 확립된 최적 HPLC 분석조건의 최소 검출한계(LOD, Limit of Detection)를 평가하기 위해 검출기의 signal/noise ratio = 3인 조건에서 최소 검출한계를 조사한 결과 3종의 검정콩 종피 함유 안토시아닌 색소의 최소 검출한계는 최소 10 ppb(0.01 ppm, 10 ng/mL) 이하에서도 완벽하게 검출되는 양상을 나타내므로 확립된 최적의 HPLC 분석조건은 검정콩 함유 안토시아닌의 작물학적 특성 평가용으로 활용하기에 전혀 무리가 없는 분석법으로 조사되었다.

• 생약학회지
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• 제39권3호
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• pp.199-202
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• 2008

A high performance liquid chromatographic (HPLC) method for the simultaneous determination of hesperidin and glycyrrhizin was established for the quality control of traditional herbal medicinal preparation, Pyungwi-san (PWS). Separation and quantification were successfully achieved with a Waters XTerra RP18 column ($5{\mu}m$, 4.6 mm I.D. ${\times}$ 150 mm) by gradient elution of a mixture of acetonitrile and water containing 0.03% phosphoric acid (pH 2.03) at a flow rate of 1.0 ml/min. The diode-array UV/vis detector (DAD) was used for the detection and the wavelength for quantification was set at 230 nm. The presence of hesperidin and glycyrrhizin in this extract was ascertained by retention time, spiking with each authentic standard and UV spectrum. All four compounds showed good linearity $(r^2>0.995)$ in a relatively wide concentration ranges. The R.S.D. for intra-day and inter-day precision was less than 7.0% and the limits of detection (LOD) were less than 60 ng. The mean recovery of each compound was 99.0-105.6% with R.S.D. values less than 4.0%. This method was successfully applied to the determination of contents of hesperidin and glycyrrhizin in three commercial products of PWS. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial PWS products.