• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.87-87
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• 2020

In this study, we investigated the immune-enhancing activity of water extracts from Hydrangea macrophylla subsp. serrata (WE-HML). WE-HML increased cell viability and production of immunomodulators, which contributed to activating phagocytic activity in RAW264.7 cells. Inhibition of JNK and NF-κB reduced the production of immunomodulators by WE-HML. ROS inhibition suppressed the production of immunomodulators, and the activation of JNK and NF-κB signaling by WE-HML. TLR4 inhibition attenuated the production of immunomodulators, and activation of JNK and NF-κB signaling by WE-HML. In the immunosuppressed mouse model, WE-HML increased the spleen index, the levels of the cytokines, the numbers of white blood cells, lymphocytes, and neutrophils. However, WE-HML inhibited LPS-mediated overproduction of pro-inflammatory mediators in RAW264.7 cells, which indicated that WE-HML may have anti-inflammatory activity under excessive inflammatory conditions. Taken together, WE-HML may be considered to have immune-enhancing activity and expected to be used as a potential immune-enhancing agent.

• Korean Journal of Plant Resources
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• v.36 no.6
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• pp.541-548
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• 2023

In the present study, the effects of HML on lipolysis, adipocyte browning, autophagy, and proliferation were investigated. HML affected lipolysis by increasing the protein levels of ATGL and HSL, and phosphorylation levels of HSL and AMPK. Furthermore, HSL decreased the perilipin-1 levels. In addition, free glycerol content was increased by HML treatment. HML affected adipocyte browning by increasing the protein levels of UCP-1, PGC-1α, and PRDM16. In addition, HML affected autophagy by increasing the levels of LC3-I and LC3-II, and decreasing those of SQSTM1/p62. Moreover, HML affected adipocyte proliferation by suppressing the proliferation of 3T3-L1 cells due to arrest of the cell cycle via blocking the expression of β-catenin and cyclin D1. These results suggest that HML induces lipolysis, adipocyte browning, autophagy, and inhibits excessive proliferation of adipocytes.

• Journal of the Korean Society of Industry Convergence
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• v.25 no.5
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• pp.791-797
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• 2022

Using the fatigue limit (△σ_unsm) and residual stress (σ_r) of the UNSM smooth specimen, the harmless maximum crack depth (α_hml) according to the crack aspect ratio (As) was evaluated. α_hml evaluated the reliability in the relationship between the minimum crack depth (α_NDI1, α_NDI2) detectable by nondestructive inspection(NDI) and the crack depth (α₂₅, α₅₀) that reduces the fatigue limit by 25% and 50%. All α_hml was determined by the crack depth. The α_hml of 80N UNSM with high σ_r and high △σ_unsm was found to be large. σ_r in the depth direction had a much effect on α_hml. Since α_hml>α₅₀(As=0.6-0.1) and α_hml>α₂₅, α₂₅ and α₅₀(some range) can secure the safety and reliability. Since α_hml<α₅₀ (As=1. 0-0. 6), it cannot be made harmless by UNSM. So safety and reliability cannot be secured. α_NDI1 and α_NDI2 are larger than α₂₅, α₂₅ cannot be detected by NDI. α_NDI1 and α_NDI2 are smaller than α₅₀, α₂₅ can detected by NDI.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.33 no.4
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• pp.44-54
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• 2020

Objectives : In the present study, we investigated whether or not an herb mixture including Lycii fructus(HML) has attenuation effects on light stress in rats. Methods : Light stress was induced by exposure to 300 lx of constant lifht for 24 h and 1mg/kg of caffeine was injected into the rat. Rats were orally treated with distilled water of HML for 21 days at 100mg/kg/d. The changes of mortality rate, clinical signs, body, blood chemistry, melatonin and IL-2 expression were analyzed after experiment. Results : Normal weight changes were observed in all treatment groups. In addition, no changes were observed in dead animals and general symptoms. At the time of autopsy, the appearance of all groups and gross examination of major organs were observed, and no gross findings were observed. Blood biochemical tests showed that total cholesterol and HDL cholesterol levels were statistically significantly decreased in the group treated with HML compared to the stress-induced group. Melatonin was measured in the blood, but there was no significant difference in the group treated with HML compared to the group with stress-induced group, but it tended to increase. IL-2 was measured in the blood, no significant change was observed in the group treated with HML compared to the stress-induced group. Conclusions : According to the above results, HML maybe help with hematological changes expressed by caffeine and light-induced stress.

• Journal of the Korean Society of Industry Convergence
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• v.26 no.4_2
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• pp.571-576
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• 2023

In this study, the harmless crack size(a_hml) according to the residual stress depth was evaluated using the fatigue limit of SCM440 steel by quenching-tempering(QT) and induction hardening(IH), and threshold stress intensity factor of QT steel. Because the residual stress increased rapidly as the crack depth increased, a_hml was determined at the depth of all the crack aspect ratio(As) regardless of Type I-III, and a_hml also increased according to the residual stress depth. a_hml was minimal at As=1.0 and maximal at As=0.1, but was almost similar on each Type. a_hml was small the dependence on As.

• Proceedings of the Korea Contents Association Conference
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• 2019.05a
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• pp.343-344
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• 2019

본 연구는 기능성 및 일반적 캐쥬얼화간에 빠른보행과 편안한 보행 시 족저 압력에 미치는 영향을 확인하여 보고자 한다. 남성 물리치료사 8명을 대상으로 하루 간격을 두고 동일한 대상에게 일반적 캐쥬얼화와 기능성 캐쥬얼화를 착용하고 10m 편안한 보행과 빠른 보행 동안 족저 악력을 각각 측정하였다. 전체, 1번째 발가락, 2-3번째 발가락, 4-5번째 발가락, 안쪽 중족골, 바깥쪽 중족골, 안쪽 뒷꿈치, 바깥쪽 뒷꿈치로 나누어 분석하여 4 보행 조건(일반적 캐쥬얼화-편안한 보행, 기능성 캐쥬얼화-편안한 보행, 일반적 캐쥬얼화-빠른 보행, 기능성 캐쥬얼화-빠른 보행) 간에 비교하였다. 비교 분석 결과 유의하지 않지만 빠른 보행이 편안한 보행 보다 안쪽 발가락과 뒷꿈치 압력이 증가하는 것으로 볼 때, 기능성 캐주얼화가 일반적 캐쥬얼화보다 보행의 움직임성을 증가시키는 것을 확인할 수 있었다.

• Proceedings of the Korea Contents Association Conference
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• 2019.05a
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• pp.341-342
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• 2019

본 연구는 기능성 신사화 착용이 즉각적인 선자세 족저압력 분포 변화에 미치는 영향을 확인하고자 하였다. 남성 물리치료사 8명을 대상으로 하루 간격을 두고 동일한 대상에게 일반적 신사화와 기능성 신사화를 착용하고 선 자세 동안 높은 족저압력의 발과 낮은 족저압력의 발간에 족저압력 분포를 비교하였다. 비교 분석 결과, 일반화를 착용한 선 자세에서 높은 족저압력의 발과 낮은 족저압력의 발간에 족저압력 비율이 유의한 차이를 보였지만 기능성 신사화에서 유의한 차이를 보이지 않았다. 그리고 높은 족저압력 분포 발에서 기능성 및 일반적 신사화간에 전족부와 후족부의 압력이 차이를 보였다. 선 자세에서 기능성 신사화가 일반적 신사화 보다 즉각적으로 정상 생리적 자세를 유도하는 것으로 보였다.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.3
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• pp.490-500
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• 2020

Objective: To investigate effect of mealworm (Tenebrio molitor) larvae hydrolysate on nutrient ileal digestibility compared to those of dried mealworm larvae meal, fermented poultry by-product, and hydrolyzed fish soluble in growing pigs. Methods: A total of 12 crossbred ([Landrace×Yorkshire]×Duroc) growing pigs with average body weight of 28.70±0.32 kg were surgically equipped with simple T-cannulas. A total of 12 pigs were assigned to individual metabolic crates and allotted to one of four treatments with 3 replicates in a fully randomized design. Results: Apparent ileal digestibility (AID) of dry matter (DM) was the highest in pigs fed HML diet. AIDs of crude protein (CP) were higher in pigs fed HML and DMLM diets than those in pigs fed the other two diets. AID of total amino acid was higher (p = 0.06) in pigs fed HML diet. AIDs of lysine (Lys), methionine (Met), and threonine (Thr) were similar in pigs fed DMLM and HML diets, but were higher (p = 0.05, p<0.05, and p = 0.05, respectively) than those in pigs fed FPBM or HFS diet. Pigs fed HML diet had higher standardized ileal digestibilities (SIDs) of DM and CP (p<0.05 and p<0.05, respectively) compared to pigs fed the other FPBM and HFS diets. SIDs of total amino acid were not different (p = 0.06) between treatments. For SIDs of Lys, Met, and Thr, pigs fed HML and DMLM diets showed higher SIDs (p = 0.05, p<0.05, and p<0.05, respectively) than pigs fed FPBM and HFS diets. SIDs of non-essential amino acids (aspartic acid, glycine, and alanine) were higher (p<0.05, p<0.05, and p<0.05, respectively) in pigs fed HML, FPBM, and DMLM diets than those in pigs fed the HFS diet. AID and SID of glutamic acid were higher in pigs fed HML and FPBM diets. Conclusion: In conclusion, dietary supplementation of mealworm larvae hydrolysate had higher digestibility in DM, CP, Lys, Met, and Thr compared to dietary supplementation with fermented poultry by-product and hydrolyzed fish soluble.

• Journal of the Korean Society of Industry Convergence
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• v.26 no.6_2
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• pp.1011-1017
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• 2023

In this study, the harmless crack size (a_hml) by double shot peening (DSP) using shot balls with different diameters was evaluated on carburized, quenched-tempered SCM822H steel. The minimum crack size (a_NDI) detectable by non-destructive inspection was also evaluated. The relationship between the crack size (a_25,50) that reduces the fatigue limit by 25% and 50% and a_hml was evaluated. The residual stress of DSP was greater in SP_(0.6+0.08) than SP_(0.8+0.08) and appeared deeper in the depth direction. In addition, the hardness below the surface appeared larger. The fatigue limit of DSP increased 2.07 times and 1.95 times compared to non-SP. All a_hml of the DSP specimen was determined at the depth (a). The compressive residual stress distribution affects a_hml, and the a_hml of SP_(0.6+0.08), which has a large compressive residual stress and a high fatigue limit, appeared large. a_hml of SP_(0.6+0.08) introduced deeper than the residual stress of SP_(0.8+0.08) is larger in the range of As=1.0-0.3. Since the residual stress in the thickness direction has a greater effect on a_hml than the residual stress at the surface, it is necessary to introduce it more deeply. The relation of a_hml, a_25,50, and a_NDI were evaluated in the point for safety and reliability.

• Biomolecules & Therapeutics
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• v.7 no.4
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• pp.307-314
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• 1999

The human muscarinic acetylcholine receptor (mAChR) subtypes Hml, Hm2 and Hm3 have been expressed in insect cells (Spodoptera frugiperda, Sf9) using the baculovirus expression system. Expression of relevant DNA, transcript and receptor proteins was identified by PCR, Northern blotting and [$^{3}H$]QNB binding, respectively. As assessed by [$^{3}H$]QNB binding sites, yields of muscarinic receptors in membrane preparations in this study were as about 5-20 times high as those in mammalian cells reported in previous studies. The [$^{3}H$]QNB competition binding studies with well-known subtype-selective mAChR antagonists showed that the receptors expressed in Sf9 cells retain the pharmacological characteristics expected for the ml , m2 and m3 muscarinic receptors. The ml-selective antagonist, pirenzepine, displayed a considerably higher affinity for Hml by 110-fold and 35-fold than for Hm2 and Hm3, respectively, The m2-selective methoctramine displayed a significantly higher affinity for Hm2 than for Hml and Hm3 (10- and 26-fold, respectively). p-F-HHSiD exhibited high affinity for Hm3 that is not significantly different from those for Hml, but 66-fold higher than its affinity for Hm2. The functional coupling of the recombinant receptors to second messenger systems was also examined. While both Hml and Hm3 stimulated phosphoinositide hydrolysis upon activation by carba-chol, Hm2 produced no response. On the other hand, activation of mAChRs induced the inhibition of forsko-lin-stimulated cyclic AMP formation in Hm2-expressing cells, whereas the significant dose-dependent increase in or poor response on cyclic AMP formation were produced in Hml or Hm3-expressing cells, respectively. These results indicate the differential coupling of recombinant Hml, Hm2 and Hm3 receptors expressed in SF9 cells to intracellular signalling system.