• Journal of KIISE:Computer Systems and Theory
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• v.35 no.2
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• pp.60-65
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• 2008

The matching preclusion set of a graph is a set of edges whose deletion results in a graph that has neither perfect matchings nor almost perfect matchings. The matching preclusion number is the minimum cardinality over all matching preclusion sets. We show in this paper that, for any $m{\geq}4$, the matching preclusion numbers of both m-dimensional restricted HL-graph and recursive circulant $G(2^m,4)$ are equal to degree m of the networks, and that every minimum matching preclusion set is the set of edges incident to a single vertex.

• Natural Product Sciences
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• v.15 no.3
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• pp.173-179
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• 2009

To determine the cytotoxic activity of natural compounds, chromatographic separation of the hexanesoluble fraction from the fruiting body of Ganoderma lucidum led to the isolation of four steroids and one triterpenoid. They were identified as ergosterol peroxide (1), stella sterol (2), ergosterol (3), 9(11)-dehydroergostrol peroxide (4), and ganodermanontriol (5) based on spectroscopic evidence and physicochemical properties. These compounds were examined for their cytotoxic activity against HL-60, MCF-7, and LLC cancer cell lines. Ganodermanontriol (5) showed cytotoxic activity with IC$_{50}$ values of 24.8 and 22.9 $\mu$g/mL against HL-60 and MCF-7 cancer cell lines, respectively, whereas compounds 1 - 4 were inactive.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.336.3-337
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• 2002

We previously reported that a synthetic naphthoquinone analog. 2.3-dichloro-5.8-dihydroxy-1, 4-naphthoquinone (NA). effectively induces apoptosis in human leukemic HL-60 cells. However. the cellular mechanism by which NA induces cell death remain unclear. In this study. we show that NA induces activation of capases. release of cytochrome c and upregulation of proapoptotic Bax protein. Futhermore. NA suppressed phosphorylation of Akt and Bad. suggesting that Akt regulates NA-induced apoptosis. Expresson of a dominant negative Akt enhancde NA-induced apoptosis. suggesting that naphthoquinone analog induces apoptosis through activating proapoptotic pathway and by the inactivation of antiapoptotic pathway.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.288.3-289
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• 2003

Sphingolipid species are important second messengers due to their role in the mitogenesis, differentiation and apoptosis. We developed a new column liquid chromatography-triple quadrupole tandem mass spectrometry (LC-MS/MS) in combination with multiple reaction monitoring (MRM) method for the rapid, simultaneous and quantitative determination of unambiguous detecting sphingolipids in cell culture of human cancer cells (HL-60). (omitted)

• Archives of Pharmacal Research
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• v.28 no.1
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• pp.87-92
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• 2005

The cyclin-dependent kinase inhibitor$p27^{kip1}$(p27) has been implicated in the regulation of cell cycle and apoptosis. Recently, we have demonstrated that ceramide induces apoptotic cell death associated with increase in the level of p27 in HL-60 cells. In the present study, we showed that overexpression of p27 increases ceramide-induced apoptotic cell death in HL-60 cells. Furthermore, overexpression of p27 accelerated DNA fragmentation, PARP cleavage and cytochrome c release induced by ceramide. In addition, ceramide induced Sax expression independent of p27. These findings indicate that enhanced effect on apoptosis by p27 is associated with mitochondrial signaling which involves cytochrome c release.

• Natural Product Sciences
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• v.17 no.2
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• pp.80-84
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• 2011

Five known compounds, cimigenol (1), 25-O-acetylcimigenol (2), cimigenol 3-O-${\beta}$-D-xylopyranoside (3), ferulic acid methyl ester (4), and visnagin (5), were isolated from Cimicifugae Rhizoma (Ranunculaceae). The chemical structures of these compounds were determined on the basis of spectroscopic analyses including 2D NMR. Compounds 1 - 5 were evaluated for their cytotoxic activity against the HL-60, MCF-7, and A549 cancer cell lines in in vitro. Among them, compounds 4 and 5 showed moderate inhibitory activity against HL-60 cancer cell lines with $IC_{50}$ values of 24.8 and 18.1 ${\mu}M$, respectively.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.148-148
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• 1993

본 실험에서는 유전자 재조합으로 제조한 [$^{125}$ I]-labeled human GM-CSF을 사용하여 GM-CSF의 HL-60 (promyelocytic leukemic cell)의 표면에 존재하는 GM-CSF의 수용체의 특성을 알고 GM-CSF의 수용체에 어떤 parameter로 결합하는지를 밝히고 나아가 현재 사용되고 있는 유전자 재조합으로 제조된 Prokine(Sargramostim)과 Lucky에서 제조된 GM-CSF (LDB-005)의 수용체에 대한 결합율을 측정, 비교하고자 하였다. 본 실험의 결과를 보면 유전자 재조립으로 제조된 Human[$^{125}$ I] GM-CSF가 HL-60 cell에 대하여 선택적으로 결합하고 표면수용체에 saturable하게 결합함을 알 수 있었으며 scatchard analysis 결과한 종류의 GM-CSF의 K3값은 2.03$\times$$10^{9}$/M로($IC_{50}$/=~493pM) 세포당 결합부위의 수는 75개 정도로 J. DiPersio et al과 Linda S. Park et al.의 보고와 비슷한 결과를 얻었다.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2003.10a
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• pp.115-115
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• 2003

• Natural Product Sciences
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• v.13 no.2
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• pp.110-113
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• 2007

Five flavonoid derivatives, pinostrobin (1), pinocembrin (2), alpinetin (3), cardamonin (4) and boesenbergin A (5) were isolated from the rhizomes of Boesenbergia pandurata. All compounds were elucidated based on its spectroscopic data and by the comparison with the previous works. 2D NMR technique was used for the structure elucidation of boesenbergin A to complement the data reported previously. The extracts and pure compounds were screened for cytotoxic activity against HL-60 cancer cell lines (human promyelocytic leukemia). Cytotoxic screening showed most of the extracts and pure compounds isolated from the rhizomes of Boesenbergia pandurata were active against HL-60 cancer cell line. The chloroform extract and boesenbergin A showed the most potent cytotoxic activity.

• Proceedings of the Korean Society of Toxicology Conference
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• 2003.10b
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• pp.115-115
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• 2003

The chloromethyl-2-dihydroxyphosphinyl-6, 7-dimethoxy-1,2,3,4-tetrahydro isoquino- line (CDDT) is a newly synthesized agent which is derivated from 1,2,3,4- Tetrahydroiso- quinoline (TIQ). The TIQs include potent cytotoxic agents that display a range of antitumor activities, antimicrobial activity, and other biological properties. (omitted)