• 한국동물위생학회지
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• 제43권1호
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• pp.23-29
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• 2020

Hepatitis E Virus (HEV) infection is a worldwide disease and the primary cause of acute viral hepatitis in the world. It can be isolated from many different species including pigs. HEV is a zoonotic pathogen and foodborne disease. The main animal reservoir is domestic pigs. It is usually asymptomatic in pig but it is a public health concern, causing acute hepatitis in humans of varying severity. This study focused on the presence of HEV in pig and pork product. One hundred feces and one hundred fifty serum samples were randomly collected from pigs in slaughterhouses in Gwangju from November in 2018 to February in 2020. In addtion, seventy-five pork products were collected from markets in Gwangju. Feces and pork product samples were examined for the presence of HEV RNA using an reverse-transcription realtime PCR (RT-qPCR) assay. Serum samples were tested for the presence of HEV-specific IgG antibodies using Enzyme-linked immunosorbent assay (ELISA). HEV antigen and antibody positive rates were 3.0% (3/100) and 19.3% (29/150), respectively, in Gwangju and nearby areas such as Jeonnam and Jeonbuk. However, HEV antigen was not detected from any of pork product in this study. In conclusion, the prevalence of HEV should be continuously monitored because HEV was sporadically detected in Gwangju and nearby areas.

• 한국산학기술학회논문지
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• 제11권12호
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• pp.4691-4696
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• 2010

본 논문에서는 HEV UV단자를 자동화 모듈인 씨마트론 다이 디자인을 활용하여 스트립 레이아웃설계를 3D로 하였다. 제품의 스탬핑을 원활하게 하기 위하여 스트립 레이아웃을 33.5도 경사지게 수정하여 광폭 1열 1개 뽑기의 내측캐리어를 단 배열로 블랭크 레이아웃을 최적화하였다. 1개의 금형에서 두개의 단자를 공용으로 양산하기 위해 29개 공정으로 3D 스트립 레이아웃설계 및 금형설계를 완성하였다.

• Journal of Veterinary Science
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• 제23권3호
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• pp.33.1-33.10
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• 2022

Background: Endemic circulation of human-specific hepatitis E virus (HEV) genotypes 1 and 2 may occult the importance of sporadic zoonotic HEV transmissions in Africa. Increasing numbers of studies reporting anti-HEV antibodies in cattle and the discovery of infectious HEV in cow milk has raised public health concern, but cattle exposure has seldom been investigated in Africa. Objectives: This study aimed at investigating the role of cows in the epidemiology of HEV in Burkina Faso and farmers habits in terms of dairy product consumption as a prerequisite to estimate the risk of transmission to humans. Methods: Sera from 475 cattle and 192 pigs were screened for the presence of anti-HEV antibodies while HEV RNA in swine stools was detected by reverse transcription polymerase chain reaction. Data on mixed farming, dairy product consumption and selling habits were gathered through questionnaires. Results: The overall seroprevalence in cattle was 5.1% and herd seroprevalence reached 32.4% (11/34). Herd seropositivity was not associated with husbandry practice or presence of rabbits on the farms. However, herd seropositivity was associated with on-site presence of pigs, 80.7% of which had anti-HEV antibodies. The majority of farmers reported to preferentially consume raw milk based dairy products. Conclusions: Concomitant presence of pigs on cattle farms constitutes a risk factor for HEV exposure of cattle. However, the risk of HEV infections associated with raw cow dairy product consumption is currently considered as low.

• 한국기계가공학회지
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• 제11권3호
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• pp.116-121
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• 2012

In recent years, world is faced with a transportation energy dilemma, and the transportation is dependent on a single fuel - petroleum. However, Hybrid Electric Vehicle(HEV) technology holds more advantages to reduce the demand for petroleum in the transportation by efficiency improvements of petroleum consumption. Therefore, there is a trend that lower gear noise levels are demanded in HEV for drivers to avoid annoyance and fatigue during operation. And meshing transmission error(T.E.) is the excitation that leads to the tonal noise known as gear whine, and radiated gear whine is also the dominant source of noise in the whole gearbox. This paper presents a method for the analysis of gear tooth profile and lead modification, and the predictions of transmission error and load distribution are shown under one loaded torque for the 1st gear pair of HEV gearbox. The test is also obtained before tooth micro-modification under the torque. At last, the appropriate tooth modification is used to minimize the transmission error and load distribution under the loaded torque. It is a good approach which the simulated result is used to improve the design in order to minimize the radiation gear whine noise.

• Agribusiness and Information Management
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• 제6권2호
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• pp.40-48
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• 2014

As basic research to develop HEV and EV agricultural field machinery, the present study analyzes the technical trend of electric agricultural field machinery through product analysis, paper analysis, and patent analysis concerning HEV and EV in the automobile, construction machinery, and agricultural machinery sectors. For product analysis, the homepages of companies in these sectors were consulted to analyze the number of products of each company. For paper analysis, key words related to HEV and EV were selected, a search formula was drawn up, and articles search sites were consulted. And for patent analysis too, key words were selected and then a search formula was drawn up to examine published patent applications or registered patent applications, and trends were analyzed by structure, country, and year. The number of HEV and EV products were 17 in the automobile area, 8 in construction machinery, and 4 in agricultural machinery. Notably, in the agricultural machinery area, all HEV and EV products were from advanced companies overseas. In terms of papers, papers published in the past 5 years were searched and 33,195 papers were from the automobile area, 3,806 were from construction machinery, and 2,687, the fewest papers, were from the agricultural machinery area. A search of patents in the electric drive technology area in Korea, USA, and Japan, and Europe showed 1,927 valid patents, with 1,120 in Japan, 497 in USA, 193 in Korea, and 117 in Europe. Analysis of the trend of research on electric agricultural field machinery by product, paper, and patent shows the development of HEV and EV technology in Korea is insufficient compared to USA, Japan, and Europe, which means rapid technological development is needed.

• Journal of Electrical Engineering and Technology
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• 제9권2호
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• pp.523-531
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• 2014

Brushless Dual-Mechanical-Port Electrical Machine (BLDMPEM) is a new type of motor designed for Hybrid Electric Vehicle (HEV), which contains two mechanical ports and two electric ports. Compared with Dual-Mechanical-Port Electrical Machine (DMPEM), the brushless structure brings higher reliability and easier maintenance. In this paper, the model of BLDMPEM is discussed. In Chapter 2, the energy flow and mathematical model of BLDMPEM are analyzed. Then a novel three-phase half-bridge controlled rectifier topology and its control strategy for cruising mode of HEV based on BLDMPEM are proposed in Chapter 3. Compared with the Field Oriented Control (FOC) strategy of BLDMPEM, the proposed method does not require accurate motor parameters, and it is much simpler and easier to be implemented. At last, simulation and experiment results show the feasibility and validity of the proposed strategy.

• International Journal of Automotive Technology
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• 제5권3호
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• pp.201-208
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• 2004

Response characteristics of the CVT system for a parallel hybrid electric vehicle (HEV) are investigated. From the experiment, CVT ratio control algorithm for the optimal engine operation is obtained. To investigate the effect of the CVT system dynamic characteristics on the HEV performance, a hardware in the loop simulation (HILS) is performed. In the HILS, hardwares of the CVT belt-pulley and hydraulic control valves are used. It is found that the engine performance by the open loop CVT ratio control shows some deviation from the OOL in spite of the RCVs open loop control ability. To improve the engine performance, a closed loop control of the CVT ratio is proposed with variable control gains depending on the shift direction and the CVT speed ratio range by considering the nonlinear characteristics of the RCV and CVT belt-pulley dynamics. The HILS results show that the engine performance is improved by the closed loop control showing the operation trajectory close to the OOL.

• Journal of Power Electronics
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• 제6권3호
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• pp.271-278
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• 2006

A new high efficiency zero-voltage and zero-current switching (ZVZCS) bidirectional DC/DC converter is proposed in this paper. The proposed converter consists of two symmetric half-bridge cells as the input and output stages. MOSFETs of input stage are turned-on in ZVS condition, and those of output stage are turned-off in ZCS condition. In addition, MOSFETs of input and output stages have low voltage stresses clamped to input and output voltage, respectively. Therefore, the proposed converter has high efficiency and high power density. The operational principles are analyzed and the advantages of the proposed converter are described. The 300W prototype of the proposed converter is implemented for 42V hybrid electric vehicle (HEV) application in order to verify the operational principles and advantages.

• 한국환경보건학회지
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• 제32권2호
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• pp.164-170
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• 2006

Ethylene oxide is a genotoxic carcinogen with widespread uses as industrial chemical intermediate and gaseous sterilant. 2-hydroxyethylated N-terminal valine in Hb is a good biomarker for biological monitoring of ethylene oxide exposure, because of its stability. For measuring the hemoglobin adduct formed by exposure of ethylene oxide, we studied the determination of (N-2-hydroxy-ethyl)valine(HEV) in hemoglobin adduct by using GC/MS. Firstly we synthesized HEV with 2-amino-ethanol and bromoisovaleric acid(BIVA) and confirmed it with GC/MS-FID. Its fragmentations were m/z 116(base ion, M+-45) and m/z 130(M+-31). For measuring HEV with higher sensitivity, we use derivatives which were PFPITH(pentafluorophenylisothiocianate) and TBDMS (tributyldimethylsilylation) by using Edman procedure. Its fragmentation were m/z 425(M+-57), m/z 383(M+-99) and m/z 172(M+-310) by using GC/MS. We did biological monitoring for mice inhalation exposure with 400 ppm ethylene oxide. The concentrations of hemoglobin adduct were $168{\pm}3.8\;and\;512{\pm}04$(nmol g-1 globin) at 0.5 hr/day 400 ppm ethylene oxide inhalation exposure group, and $631{\pm}17\;and\;2265{\pm}9.4$(nmol g-1 globin) at 1.0 hr/day 400 ppm ethylene oxide inhalation exposure for 1 and 4 weeks, respectively. We confirmed that (N-2-hydroxy-ethyl)valine(HEV) of hemoglobin was a good biomarker for biomonitoring of ethylene oxide exposure, and can measured with derivatives such as PFPITH(pentafluorophenylisothiocianate) and TBDMS(tributyldimethylsilylation) by using GC/MS.

• BMB Reports
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• 제39권3호
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• pp.247-252
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• 2006

A rapid method for the detection of Hepatitis E Virus (HEV) was developed by utilizing nano-gold labeled oligonucleotide probes, silver stain enhancement and the microarray technique. The 5'-end -$NH_2$ modified oligonucleotide probes were immobilized on the surface of the chip base as the capture probe. The detection probe was made of the 3'-end -SH modified oligonucleotide probe and nano-gold colloid. The optimal concentrations of these two probes were determined. To test the detection sensitivity and specificity of this technique, a conservative fragment of the virus RNA was amplified by the RT-PCR/PCR one step amplification. The cDNA was hybridized with the capture probes and the detection probes on microarray. The detection signal was amplified by silver stain enhancement and could be identified by naked eyes. 100 fM of amplicon could be detected out on the microarray. As the results, preparation of nano-gold was improved and faster. Development time also was shortened to 2 min. Thus, considering high efficiency, low cost, good specificity and high sensitivity, this technique is alternative for the detection of HEV.