• Journal of Life Science
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• v.23 no.8
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• pp.978-983
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• 2013

Kinesin-II, a molecular motor, consists of two different motor subunits, KIF3A and KIF3B, and one large kinesin superfamily-associated protein 3 (KAP3), forming a heterotrimeric complex. KAP3 is associated with the tail domains of motor subunits. However, its exact role remains unclear. Here, we demonstrated KAP3 binding to the carboxyl (C)-terminal tail region of HS-associated protein X-1 (HAX-1). HAX-1 bound to the C-terminal region of KAP3, but not to KIFs (KIF3A, KIF3B, and KIF5B) and the kinesin light chain (KLC) in the yeast two-hybrid assays. The interaction was further confirmed in the glutathione S-transferase (GST) pull-down assay and by co-immunoprecipitation. Anti- HAX-1 antibody as well as anti-KIF3A antibody co-immunoprecipitated KIF3B and KAP3 from mouse brain extracts. These results suggest that KAP3 could mediate the interaction between Kinesin-II and HAX-1.

• Molecules and Cells
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• v.25 no.1
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• pp.86-90
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• 2008

Caspase-3 (CASP3) plays a key role in apoptosis. In this study, HAX-1 was identified as a new substrate of CASP3 during apoptosis. HAX-1 was cleaved by CASP3 during etoposide-(ETO) induced apoptosis, and this event was inhibited by a CASP3-specific inhibitor. The cleavage site of HAX-1, at $Asp^{127}$, was located using N-terminal amino acid sequencing of in vitro cleavage products of recombinant HAX-1. Overexpression of HAX-1 inhibited ETO-induced apoptotic cell death. It also inhibited CASP3 activity. Together, these results suggest that HAX-1, a substrate of CASP3, inhibits the apoptotic process by inhibiting CASP3 activity.

• Journal of the Korean Institute of Gas
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• v.3 no.1
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• pp.48-57
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• 1999

Safe plants are maintained by the systematic identification of potential hazards. Various hazard evaluation methods have been developed in pursuit of safe plants. Recently, much efforts has been made for the automation of hazard evaluation system by introducing expert system to get rid of weak points of the conventional hazard evaluation methods. HAZOP study is recognized as one of the most systematic and logical hazard evaluation methods. However, it has several disadvantages; experts should participate simultaneously, the detailed study is time-consuming, and the quality of the results largely depends on the quality of the experts. Therefore, the automation of HAZOP Study is highly beneficial for reducing the required time and obtaining the consistent evaluation results. In this study, a framework for the automation of HAZOP study is suggested. Based on the suggested framework, HAxSYM, an expert system to automate HAZOP study, has been developed. The case study validates the performance of the developed system, and the results are compared with the results from the conventional HAZOP study.