• Korean Journal of Environmental Agriculture
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• v.24 no.2
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• pp.117-122
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• 2005

To find out reducing way of methane emission from a paddy field monitoring on the greenhouse gases emissions were carried out in the paddy soil with livestock compost and soil conditioner. The seasonal variations of methane emission were high at 36 days and 86 days after rice transplanting, on the other hand those of nitrous oxide emission were high at 64 days after that day. Methane emission by cow compost application, pig compost application and chicken compost application were 331, 282 and 294 kg $ha^{-1}$, respectively. Otherwise, nitrous oxide emissions by cow compost application, pig compost application and chicken compost application were 1.60, 1.78 and 1.78 kg $ha^{-1}$ respectively. The total emission rate of greenhouse gases equivalent to $CO_2$ emission rate (GWP) was 7,447 kg $ha^{-1}$ in cow compost application, 6,474 kg $ha^{-1}$ in pig compost application and 6,726 kg $ha^{-1}$ in chicken compost application. Methane emission by Ca, $SiO_2$ and artificial zeolite application were 373, 264, 239 kg $ha^{-1}$, respectively. The total emission rate of greenhouse gases equivalent to $CO_2$ emission rate (GWP) was 8,295 kg $ha^{-1}$ in Ca application 5,978 kg $ha^{-1}$ in $SiO_2$ application and 5,447 kg $ha^{-1}$ in artificial zeolite application.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.1
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• pp.1-6
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• 2003

This study was determined to the growth characteristic, yield and chemical content of Azuki bean(Vigna angularis W.F. $W_{IGHT}$) based on five phosphate rates (0, 40, 50, 120, 160, 200kg/ha) from May, 2002 to August, 2002 in Jeju province. Plant height was 80.7cm at 0kg/ha of phosphate level md, as phosphate rates were increased to 160kg/ha and 200kg/ha, lengthened 88.8cm and 88.9cm, respectively, but between the two phosphat levels were no significance(P＞0.05 or 0.0l). Numbs. of branches and leaves per plant, stem diameter, and weight of leaves and stems per plant were the same response with plant height. Fresh forage, dry matter, crude protein, and TDN(total digestible nutrient) yield at the control were 23.8MT/ha, 3MT/ha, 0.5MT/ha and 1.8MT/ha, respectively, at the 200kg/ha plot were 47.3MT/ha, 7.2MT/ha, 1.3MT/ha, and 4.7MT/ha, respectively, as phosphate rate was increased. As phosphate rate increased from 0kg/ha to 200kg/ha, the content of crude protein, crude fat NFE(nitrogen free extract) and TDN increased 15.2%~18.6%, 3.4%~4.5%, 41.4%~45.5% and 58.3%~65.5%, respectively, whereas the content of crude fiber and crude ash were decreased 31.5%~24.8% and 8.1%~6.6%, respectively.

• Journal of Korean Society of Forest Science
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• v.98 no.6
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• pp.772-779
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• 2009

This study was conducted to estimate the carbon (C) contents in pure and mixed stands of pine (Pinus densiflora) and oak (Quercus spp.) trees for establishing the C inventory of forest ecosystems. A total of fifteen 20 m${\times}$20 m pure and mixed stands of pine and oak trees were chosen in natural forests in Hoengseong, Kangwon based on the basal area of all trees ${\geq}$ 5 cm DBH: three of 95% of pine and 5% oak trees [pine stand], three of 100% of oak trees [oak stand], and nine of 20 to 70% of pine and 80 to 30% of oak trees [mixed stand]. To estimate C contents in the study stands, biomass in vegetation, forest floor and coarse woody debris (CWD) were calculated and C concentrations in vegetation, forest floor, CWD and soil (0-30 cm) were analyzed. There was no significant difference in vegetation C contents among the stands; 147.6 Mg C/ha for the oak stand, 141.4 Mg C/ha for the pine stand and 115.8 Mg C/ha for the mixed stand. Forest floor C contents were significantly different among the stands (p<0.05); 12.7 Mg/ha for the pine stand, 9.9 Mg/ha for the oak stand, and 8.4 Mg/ha for the mixed stand. However, CWD C contents were not significantly different among the stands (p>0.05); 2.2 Mg/ha for the mixed stand, 1.7 Mg/ha for the oak stand, and 1.1 Mg/ha for the pine stand. Soil C contents up to 30 cm depth were not significantly different among the study stands; 44.4 Mg C/ha for the pine stand, 41.6 Mg C/ha for the mixed stand, and 33.3 Mg C/ha for the oak stand. Total ecosystem C contents were lower in the mixed stand than those in the pure stands, because vegetation C contents which occupied almost total ecosystem C contents were lower in the mixed stand than those in the pure stands; 199.6 Mg C/ha for the pine stand, 192.5 Mg C/ha for the oak stand and 169.1 Mg C/ha for the mixed stand. Lower vegetation C contents in the mixed stand might be influenced by interspecific competition between pine and oak trees and intraspecific competition among the oak trees resulted from high stand density. We suggest that forest management such as thinning to enhance C storage is indispensible for minimizing the competition in forest ecosystems.

• Korean Journal of Organic Agriculture
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• v.21 no.2
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• pp.247-263
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• 2013

This study was conducted to investigate the effect of cattle manure application on productivity, feed value, and stock carrying capacity of forage crops in upland and paddy fields at Gang-Wondo area. In the result, dry matter yield of sorghum ${\times}$ sudangrass hybrids obtained was 15.12 ton/ha at the level of 150kg N/ha of composted cattle manure. Significantly highest values of crude protein and total digestible nutrients (TDN) yields obtained were 0.59 and 5.35 ton/ha at the level of 150kg N/ha of composted cattle manure in the first cutting, and 0.44 and 3.70 ton/ha at the level of 150kg N/ha of organic raw cattle manure in the second cutting, respectively. The values of $K_{CP}+K_{TDN}/2$ and $K_{ME}$ of sorghum ${\times}$ sudangrass hybrids obtained was 7.76 and 4.46 head/ha at the level of 150kg N/ha of composted cattle manure. The dry matter yield, crude protein and TDN yields of rice straw were 4.95, 0.16 and 2.75 ton/ha at the level of 100kg N/ha of organic raw cattle manure, and the values of $K_{CP}+K_{TDN}/2$ and $K_{ME}$ of rice straw were 1.89 and 3.43 head/ha. The dry matter yield of winter crops, rye+red clover was 4.36 ton/ha in upland field, and rye+hairy vetch was 4.19 ton/ha in paddy field at the level of 100kg N/ha of composted cattle manure. Crude protein and TDN yields of rye+red clover was 0.29 and 2.38 ton/ha at the level of 100kg N/ha of composted cattle manure in upland field, and rye+hairy vetch was 0.30 and 2.48 ton/ha at the level of 80kg N/ha of composted cattle manure in paddy field. The values of $K_{CP}+K_{TDN}/2$ and $K_{ME}$ of rye+red clover was 2.34 and 2.15 head/ha in upland field, and rye+hairy vetch were 2.27 and 2.11 head/ha in paddy field, respectively. As the result, the productivity, feed value, and stock carrying capacity of sorghum ${\times}$ sudangrass hybrids showed higher values with composted cattle manure than organic raw cattle manure. rye+red clover in upland field and rye+hairy vetch in paddy field were most adaptable mixed combinations for roughage production at Gang-wondo area, it may due to their highly productivity, feed value, and stock carrying capacity.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.14 no.3
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• pp.83-89
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• 2004

In this work, HA/$TiO_2$ biocomposite to get high mechanical properties with biocompatibility were prepared. HA/$TiO_2$ biocomposite powders were prepared by mixing $TiO_2$ and HA powders which were synthesized through sol-gel, precipitation and hydrothermal methods. The mixing ratio was fixed at 1:1 ratio (HA/$TiO_2$, wt%). HA/$TiO_2$ biocomposite powders showed different microstructures depending on their particle size and shape. The smaller particles were coated on the surface of larger particles, whereas they were well mixed and dispersed when both $TiO_2$ and HA were nanocrystallites. HA/$TiO_2$ biocomposite powders with homogeneous microstructure showed high sintered density and good mechanical properties.

• YAKHAK HOEJI
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• v.55 no.1
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• pp.69-74
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• 2011

Hyaluronic acid (HA) is a natural polymer consisting of disaccharide units of D-glucuronic acid and N-acetyl-D-glucosamine. It has a great potential and success in cosmetic and biomedical applications. However, native HA is highly soluble and easily metabolized by enzymes such as hyaluronidase. Thus, various studies have been reported on modifying the physicochemical properties of HA, while maintaining its biocompatibility. For controlled drug delivery, many trials for fabricating HA microspheres were achieved under chemical reaction. The HA microspheres fabricated to improve the physical stability of HA using adipic acid dihydrazide (ADH) by cross-linking reaction has been reported earlier, however it lacks the desired physical stability and rapidly decomposes by swelling or enzymes. Therefore, we prepared double cross-linked HA microspheres (DC-HA microspheres) and alginate containing HA microspheres (AC-HA microspheres) to enhance its physicochemical properties. DC-HA microspheres were prepared using trisodium trimetaphosphate (STMP) under crosslinking reaction after ADH cross-linking reaction. AC-HA microspheres were prepared by adding alginate as a networking polymer. These microspheres were characterized by morphology, particle size, zeta potential, stability against hyaluronidase. Results showed that the DC-HA and AC-HA microspheres are more stable than that of HA microspheres.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.157-160
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• 2011

This study was conducted to find a method using the application of rice bran and mixed expeller cake at machine transplanting rice paddy field. Different ratios of rice bran and mixed expeller cake were sprayed as substitute of chemical fertilizer (nitrogen 90kg $ha^{-1}$) before transplanting. Nitrogen content was highest in 30th day after transplantation, and in relation to treatments the order was the following: Rice bran 1,000kg $ha^{-1}$ + Mixed expeller cake 1,374kg $ha^{-1}$ > rice bran 2,000kg $ha^{-1}$ + Mixed expeller cake 948kg $ha^{-1}$ > rice bran 3,000kg $ha^{-1}$ + Mixed expeller cake 522kg $ha^{-1}$. Number of panicle and spikelets per $m^{-2}$ was higher in rice bran 1,000kg $ha^{-1}$ + Mixed expeller cake 1,374kg $ha^{-1}$ and rice bran 2,000kg $ha^{-1}$ + Mixed expeller cake 948kg $ha^{-1}$ than in rice bran 3,000kg $ha^{-1}$ + Mixed expeller cake 522kg $ha^{-1}$ and the yields was the highest in rice bran 1,000kg $ha^{-1}$ + Mixed expeller cake 1,374kg $ha^{-1}$.

• Polymer(Korea)
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• v.32 no.5
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• pp.415-420
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• 2008

The porcine small intestinal submucosa (SIS) without immunorejection responses and hyalunonic acid (HA) can be used as biomaterials. In this study, we tried to design and characterize novel sponge. SIS- HA sponge was prepared by freeze-drying after addition 1wt% HA solution into fabricated SIS sponge. Sponge was crosslinked with 1-ethyl-(3-3-dimethyl aminopropyl) carbodiimide hydrochloride (EDC) solution with 100mM concentration for 24 hrs and lyophilized. SIS-HA sponge was characterized by scanning electron microscopy and fourier transform infrared spectrometer. And water absorption ability of sponge was evaluated. We seeded NIH/3T3 cells in SIS-HA sponge and cellular attachment was assayed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltertazolium-bromide (MTT) test. We demonstrated presence of HA in SIS-HA sponge from C-O functional group observed by the FT-IR analysis. Moreover, we confirmed low cytotoxicity and high cell viability of the SIS-HA sponges. Therefore, we could expect that SIS- HA scaffolds are applicable for the tissue regeneration.

• The Journal of Korean Academy of Prosthodontics
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• v.57 no.1
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• pp.88-94
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• 2019

Increasing demands for esthetic dental treatment, zirconia, which has high mechanical and esthetic properties, had been applied more and more in clinics. Therefore, assessment of biocompatibility of zirconia is necessary. In this article, a review of in vivo studies of zirconia compatibility was performed. In vivo studies showed zirconia had great biocompatibility both on soft and hard tissue. Studies with various animals and patients reported high biocompatibility of zirconia. In terms of bone synthesis and bone adhesion, zirconia showed similar biocompatible properties to titanium. On the other hand, zirconia could be used as implant. For using as an implant, various methods of Hydroxyapatite (HA) coating had been suggested. Since HA coating on titanium implant showed some problems such as low bonding strength and degeneration of HA, HA-zirconia composite, HA-coated zirconia, and HA-zirconia functionally graded material (FGM) or intermediate layer of alumina had been proposed. These methods showed higher bonding strength and biocompatibility.

• Journal of Periodontal and Implant Science
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• v.53 no.4
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• pp.259-268
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• 2023

Purpose: Hyaluronic acid (HA) affects angiogenesis and promotes the migration and differentiation of mesenchymal cells, thereby activating the osteogenic ability of osteoblasts. Although studies on the action of HA during bone regeneration are being actively conducted, the optimal dose of HA required for bone regeneration remains unclear. Therefore, the purpose of this study was to elucidate the most effective HA dose for bone formation using a rat critical-size defect model. Methods: Thirty rats were randomly divided into 5 groups, with 6 rats in each group. An absorbable collagen sponge soaked with HA or saline was used to fill an 8-mm defect, which was then covered with a collagen membrane. Different treatments were performed for each group as follows: (1) saline control, (2) 1 mg/mL HA, (3) 25 mg/mL HA, (4) 50 mg/mL HA, or (5) 75 mg/mL HA. After a healing period of 4 weeks, micro-computed tomography and histological analysis were performed. The obtained values were analyzed using analysis of variance and the Tukey test (P<0.05). Results: At week 4, the 75 mg/mL HA group had the highest bone volume/total volume ratio, new bone, and bone fill among the 5 groups, and these values were significantly different from those observed in the control group (P<0.01) and 1 mg/mL HA group (P<0.001). More active bone formation was observed in the higher-dose HA groups (25 mg/mL, 50 mg/mL, and 75 mg/mL HA), which included a large amount of woven bone. Conclusions: The 75 mg/mL HA group showed better bone formation than the other groups (1, 25, and 50 mg/mL HA and control).