Browse > Article

Preparation of Hyaluronic Acid Microspheres with Enhanced Physical Stability by Double Cross-link or Alginate  

Kim, Dong-Hwan (College of Pharmacy, Seoul National University)
Song, Chung-Kil (College of Pharmacy, Seoul National University)
Balakrishnan, Prabagar (College of Pharmacy, Seoul National University)
Park, Chun-Geon (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
Choi, Ae-Jin (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
Chung, Suk-Jae (College of Pharmacy, Seoul National University)
Shim, Chang-Koo (College of Pharmacy, Seoul National University)
Kim, Dae-Duk (College of Pharmacy, Seoul National University)
Publication Information
YAKHAK HOEJI / v.55, no.1, 2011 , pp. 69-74 More about this Journal
Abstract
Hyaluronic acid (HA) is a natural polymer consisting of disaccharide units of D-glucuronic acid and N-acetyl-D-glucosamine. It has a great potential and success in cosmetic and biomedical applications. However, native HA is highly soluble and easily metabolized by enzymes such as hyaluronidase. Thus, various studies have been reported on modifying the physicochemical properties of HA, while maintaining its biocompatibility. For controlled drug delivery, many trials for fabricating HA microspheres were achieved under chemical reaction. The HA microspheres fabricated to improve the physical stability of HA using adipic acid dihydrazide (ADH) by cross-linking reaction has been reported earlier, however it lacks the desired physical stability and rapidly decomposes by swelling or enzymes. Therefore, we prepared double cross-linked HA microspheres (DC-HA microspheres) and alginate containing HA microspheres (AC-HA microspheres) to enhance its physicochemical properties. DC-HA microspheres were prepared using trisodium trimetaphosphate (STMP) under crosslinking reaction after ADH cross-linking reaction. AC-HA microspheres were prepared by adding alginate as a networking polymer. These microspheres were characterized by morphology, particle size, zeta potential, stability against hyaluronidase. Results showed that the DC-HA and AC-HA microspheres are more stable than that of HA microspheres.
Keywords
hyaluronic acid; microsphere; cross-linker; adipic acid dihydrazide; trisodium trimetaphosphate; alginic acid;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Freed, L. E., Vunjak-Novakovic, G., Biron, R. J., Eagles, D. B., Lenov, D. C., Barlow, S. K. and Langer, R. : Biodegradable polymer scaffolds for tissue engineering. Biotechnology 12, 689 (1994).   DOI   ScienceOn
2 Kang, J. Y., Chung, C. W., Sung, J. H., Park, B. S., Choi, J. Y., Lee, S. J., Choi, B. C., Shim, C. K., Chung, S. J. and Kim, D. D. : Novel porous matrix of hyaluronic acid for the threedimensional culture of chondrocytes. Int. J. Pharm. 369, 114 (2009).   DOI
3 Yunand, Y. : Hyaluronan microspheres for sustained gene delivery and site-specific targeting. Biomaterials 25, 147 (2004).   DOI   ScienceOn
4 Zhao, X. B., Fraser, J. E., Alexander, C., Lockett, C. and White, B. J. : Synthesis and characterization of a novel double crosslinked hyaluronan hydrogel. J. Mater. Sci. 13, 11 (2002).
5 Zawko, S., Suri, S., Truong, Q. and Schmidt, C. : Photopatterned anisotropic swelling of dual-crosslinked hyaluonic acid hydrogels. Acta Biomater. 5, 14 (2009).   DOI   ScienceOn
6 Dulong, V. : Hyaluronan-based hydrogels particles prepared by crosslinking with trisodium trimetaphosphate. Synthesis and characterization. Carbohydr. Polym. 57, 1 (2004).   DOI   ScienceOn
7 Scott, A., Zawko, Shalu S., Quan, T. and Chrestine, E. Schmidt : Photopatterned anisotropic swelling of dual cross-linked hyaluronic acid hydrogels. Acta Biomater. 5, 14 (2009).   DOI   ScienceOn
8 Zhao, X. B., Fraser, J. E., Alexander, C., Lockett, C. and White, B. J. : Synthesis and characterization of a novel double crosslinked hyaluronan hydrogel. J. Mater. Sci. 13, 11 (2002).
9 Cheon, J. W., Shim, C. K., Chung, S. J. and Kim, D. D. : Effect of tripolyphosphate (TPP) on the controlled release of cyclosporin a from chitosan-coated lipid microparticles. J. Kor. Pharm. Sci. 39, 59 (2009).
10 Laurent, T., Laurent, U. and Fraser, J. R. : Functions of hyaluronan. Ann. Rheum. Dis. 54, 429 (1995).   DOI   ScienceOn
11 Laurent, T., Laurent, U. and Fraser, J. R. : The structure and function of hyaluronan: An overview. Immunol. Cell Biol. 74, A1 (1996).   DOI
12 Chen, W. and Abatangelo, G. : Functions of hyaluronan in wound repair. Wound Repair Regen. 7, 79 (1999).   DOI   ScienceOn
13 Scott, J. : Extracellular matrix, supramolecular organisation and shape. J. Anat. 187, 259 (1995).
14 Collis, L., Hall, C., Lange, L., Ziebell, M., Prestwich, R. and Turley, E. : Rapid hyaluronan uptake is associated with enhanced motility: implications for an intracellular mode of action. FEBS Letters 440, 444 (1998).   DOI   ScienceOn
15 Toole, B. : Hyaluronan in morphogenesis, Semin. Cell Dev. Biol. 12, 79 (2001).   DOI   ScienceOn
16 Gerdinand, B. and Hallgren, R. : Dynamic role of hyaluronan (HYA) in connective tissue activation and inflammation. J. Intern. Med. 242, 49 (1997).   DOI   ScienceOn
17 Kim, A., Checla, D. M. and Chen, W. : Characterization of DNAhyaluronan matrix for sustained gene transfer. J. Control. Release 90, 81 (2003).   DOI   ScienceOn