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Effect of Timed Artificial Insemination Protocols on the Pregnancy Rate Per Insemination and Pregnancy Loss in Dairy Cows and Korean Native Cattle under Heat Stress

  • Uhm, Hyun-Boem;Jeong, Jae-Kwan;Kang, Hyun-Gu;Kim, Ill-Hwa
    • Journal of Veterinary Clinics
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    • v.37 no.5
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    • pp.235-241
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    • 2020
  • We aimed to determine the effect of timed artificial insemination (TAI) protocols on the pregnancy rate per insemination and pregnancy loss compared with AI performed at detected estrus in dairy cows and Korean Hanwoo cattle under heat stress. In dairy cattle, 1,250 sets of data that underwent AI during heat stress (temperature-humidity index ≥ 72) were categorized according to their TAI protocols or as controls: 1) PGF-36 h-estradiol benzoate (EB)-36 h-TAI (PG-EB group, n = 113); 2) GnRH-7 days-PGF-56 h-GnRH-16 h-TAI (Ovsynch group, n = 455); or 3) GnRH-6 days-Ovsynch (G6G group, n = 136). The remaining cows underwent AI at detected estrus (AIDE group, n = 546). The probability of pregnancy per AI 45 days after AI was higher (P < 0.01) in the PG-EB (odds ratio [OR]: 1.68), Ovsynch (OR: 1.48), and G6G (OR: 1.79) groups than in the AIDE group. However, the prevalence of pregnancy loss between 30 and 45 days after AI did not differ among the groups. In Hanwoo cattle, 617 sets of data inseminated artificially under heat stress were categorized into AIDE (n = 281), PG-EB (n = 194), and combined Ovsynch or G6G (n = 142) groups. The probability of pregnancy per AI 45 days after AI and the prevalence of pregnancy loss between 30 and 45 days after AI did not differ among the groups. Thus, implementation of a TAI protocol (PG-EB, Ovsynch, or G6G) in dairy cows under heat stress improves the pregnancy rate per AI versus AIDE, whereas there is no beneficial effect of TAI on the pregnancy rate of Hanwoo cattle under heat stress.

Protease activity and meat-tenderizing effect of Hypsizygus marmoreus (느티만가닥버섯의 단백질분해효소 활성과 연육증진효과)

  • Shin, Bok-Eum;Baek, Il-Sun;Kim, Jeong-Han;Lee, Yun-Hae
    • Journal of Mushroom
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    • v.17 no.4
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    • pp.235-240
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    • 2019
  • In this study, we investigated the effects of pH, temperature, and salt concentration on protease activities in Hypsizygus marmoreus, and the ability of H. marmoreus extract to tenderize beef. In fact, H. marmoreus was shown to have higher protease activity than kiwi fruits. The pH of beef was dose dependently increased with addition of H. marmoreus extract. Cooking loss, cutting strength, and color value (L, a, b) were also dose dependently decreased with addition of H. marmoreus extract. In sensory assessments, H. marmoreus extract dose dependently enhanced perception of tenderness, flavor, and taste. In contrary, use of kiwi fruits as a meat tenderizer decreased positive perception of taste. The protease activitiy of H. marmoreus decreased sharply at pH < 2.0, and temperature > 50 ℃. Protease activity was relatively stable in NaCl concentrations ranging from 0-5 M, but incrementally decreased with increasing NaCl. These results suggest that, H. marmoreus extract can improve the texture and taste of beef.

Optimization for the DXAMase production from Lipomyces starkeyi using statistically designed experiments

  • Park, Jun-Seong;Gang, Hui-Gyeong;Gang, Seong-Ju;Kim, Byeong-Hun;Park, Don-Hui;Kim, Do-Man
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.211-214
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    • 2002
  • The optimal condition for the production of DXAMase, containing the both characteristics of dextranase and amylase, was studied based on different levels of pH, temperature, and aeration rate. Response surface methodology was applied to find the optimatic condition showing the relationship between the fermentation response(dextranase and amylase activity of DXAMase) and the fermentation variables(pH, temperature, and agitation rate). In case of dextranase activity, the condition of pH 4.06, $28.08^{\circ}C$, and 235.14 rpm showed the highest activity, 2.26 U/ml, and for amylase activity, the condition of pH 4.01, $27.96^{\circ}C$, and 212.01 rpm showed the highest activity, 3.52 U/ml. For the production of DXAMase, dextranase and amylase, the optimum condition was pH 4.06, $28.08^{\circ}C$, and 234.80 rpm.

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Induction of Cytotoxicity by HexamethyleneBisacetamide is Associated with the Activity of Telomerase in RBL-2H3 Cells (RBL-2H3 세포에서 hexamethylene bisacetamide의 세포독성과 telomerase 활성과의 관계)

  • Kim Young Mi
    • Environmental Analysis Health and Toxicology
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    • v.19 no.3
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    • pp.235-240
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    • 2004
  • 본 연구는 비만세포의 일종인 RBL-2H3 세포주에 대한 hexamethylene bisacetamide (HMBA)의 세포독성 유도 기전이 telomerase의 활성과 관계가 있다는 것에 대한 보고이다. RBL-2H3 세포주에 5mM HMBA를 처리시 그 세포주의 세포성장이 억제 되었다. 기전연구로서 HMBA는 telomerase활성을 억제 하였으며 RT-PCR에 의한 결과 telomerase mRNA발현의 억제에 의한 것이었다. Madl은 telomerase의 발현을 억제하는 억제인자로 잘 알려져 왔으며 이를 확인해본 결과 Madl은 발현은 유의성있게 증가 되었다. 이를 종합해보면, HMBA에 의한 RBL-2H3 세포주에 대한 세포독성은 Madl 발현의 증가에 따른 telomerase발현의 억제에 기인하는 것으로 밝혀졌다.

Design of H$\infty$ tracking control for linear systems with saturating actuators (포화구동기를 갖는 선형 시스템의 H$\infty$ 추종 제어기 설계)

  • Yi Yearn-Gui;Kim Myoung-Soon;Kim Jin-Hoon
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.54 no.4
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    • pp.235-242
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    • 2005
  • In this paper, we consider the H$\infty$ tracking control of linear system with a limited actuator capacity. The considered reference is a general time-varying one with bounded magnitude and rate. By adopting a similarity transform and a new sto variable, we convert the original system equation to new one which has a tracking error as a part of the new state variable. First, we obtain a result on the low-gained H$\infty$ tracking control which never permits the actuator saturation. Next, we give a result on scheduled H$\infty$ tracking control which uses the actuator capacity more effectively. All results are in the form of linear matrix inequalities(LMI) which can be easily checked their feasibility. Finally, we give a numerical example to show the validity and usefulness of our results.

Frequency Control of Demand Users with Power Plants by Under-frequency Relay (저주파수 계전기에 의한 수용가 발전설비 계통 주파수 제어)

  • Kim, H.M.;Kim, D.H.;Chun, Y.H.;Kim, J.W.;Kook, K.S.;Jeon, J.H.
    • Proceedings of the KIEE Conference
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    • 2001.05a
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    • pp.233-235
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    • 2001
  • This paper deals with frequency control of demand users that have power plants by under-frequency relay. The demand users supply electrical power to a part of their loads by their power plants and to other rest of their loads by utility. While electrical power supply is stopped by faults of utility network, the system of demand users network is separated from total power system and their system frequency goes below normal limits. In this paper, this situation and the effect of under-frequency relay application are simulated by EMTDC.

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Immunomodulating Effects on Macrophage of Rhamnan Sulfate Extracted from Monostroma nitidum (홀파래에서 추출한 Rhamnan sulfate의 Macrophage에 의한 면역조절효과에 관한 연구)

  • 류병호;빈재훈;김희숙
    • Journal of Food Hygiene and Safety
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    • v.11 no.4
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    • pp.235-241
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    • 1996
  • The rhamnan sulfate extracted from green algae seaweed, Monostroma nitidum was characterized as activity in vitro culture assay with macrophages from mice. Rhamnan sulfate indicated that F-4-3 fraction enhanced glucose consumption, as well as the production of nitrogen dioxide and tumor necrosis factor(TNF). F-4-3 fraction was also augmented IL-1 secretion from those macrophages. Effects of the pretreatment of peritoneal macrophages with rhamnan sulfate F-4-3 fraction and several polysaccharides as relative standard on the production of H2O2 induced with unopsonized zymosan A were examined. Pretreatment with polysaccharides inhibited the zymosan A mediated H2O2 production by macrophages. The phorbol myristate acetate (PMA) mediated H2O2 production was not affected by the pretreament. These result suggested that pretreatment of rhamnan sulfate interfered with the interaction of macrophages zymosan A. Rhamnan sulfate inhibited zymosan A mediated production of H**O** by macrophages and F-4-3 Fraction was also activator of macrophages.

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Synthesis and Characterization of Holmium Complexes Containing $\beta$-Diketonate Ligands

  • 이정해;정영숙;손윤수;강성주
    • Bulletin of the Korean Chemical Society
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    • v.19 no.2
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    • pp.231-235
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    • 1998
  • Two holmium β-diketonate complexes, Ho(hfa)3(H2O)2 (1) and [Ho(hfa)3(H2O)2](triglyme) (2), have been prepared and characterized by IR, TGA, and single-crystal X-ray analyses. These complexes show polymeric chains by the intermolecular hydrogen bondings. The donor atoms of the intermolecular hydrogen bonding in both complexes are hydrogen atoms of the coordinated water molecules. The acceptor atoms in 1 are the carbonyl oxygen atoms of β-diketonate ligands whereas those in 2 are oxygen atoms of the triglyme ligand. While compound 1 decomposes cleanly to Ho2O3, compound 2 sublimes intact. Crystal data for 1 and 2 are as follows: Ho(hfa)3(H2O)2 triclinic P1, a=10.158(4), b=11.628(2), c=12.579(6) Å, α=67.02(3)°, β=73.95(4)°, γ=76.12(2)°, V=1299.8(8) Å3. [Ho(hfa)3(H2O)2](triglyme), monoclinic P21/c, a=12.559(3), b=19.111(2), c=16.789(6) Å, β=110.59(4)°, V=3772(2) Å3.

CHANDRA OBSERVATIONS OF THE AKARI NEP DEEP FIELD

  • Miyaji, T.;Krumpe, M.;Brunner, H.;Ishigaki, T.;Hanami, H.;Markowitz, A.;Takagi, T.;Goto, T.;Malkan, M.A.;Matsuhara, H.;Pearson, C.;Ueda, Y.;Wada, T.
    • Publications of The Korean Astronomical Society
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    • v.32 no.1
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    • pp.235-237
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    • 2017
  • The AKARI NEP Deep Field Survey is an international multiwavelength survey over $0.4deg^2$ of the sky. This is the deepest survey made by the InfraRed Camera (IRC) of the infrared astronomical satellite AKARI with 9 filters continuously covering the $2-25{\mu}m$ range, including three filters in the Spitzer gap between the IRAC and MIPS coverages. This enabled us to make sensitive MIR detection of AGN candidates at z~ 1, based on hot dust emission in the AGN torus. It is also efficient in detecting highly obscured Compton-thick AGN population. In this article, we report the first results of X-ray observations on this field. The field was covered by 15 overlapping Chandra ACIS-I observations with a total exposure of ~300 ks, detecting ${\approx}450$ X-ray sources. We utilize rest-frame stacking analysis of the MIR AGN candidates that are not detected individually. Our preliminary analysis shows a marginal detection of the rest-frame stacked Fe $k{\alpha}$ line from our strong Compton-thick candidates.

Morphological study of the genus Eucampia (Bacillariophyceae) in Korean coastal waters

  • Lee, Jun Mo;Lee, Jin Hwan
    • ALGAE
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    • v.27 no.4
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    • pp.235-247
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    • 2012
  • Regardless of continuous researches, recent researches on the genus Eucampia (Bacillariophyceae) have focused mainly on E. zodiacus f. zodiacus In the present study, species of the genus Eucampia have been studied based on their morphological characteristics. Eucampia species were collected at 24 sites from July 2008 to June 2011 in Korean coastal waters. Species were mainly identified based on the shape of valve, ocellus, and aperture, along with the length and shape of the bipolar elevations. As a result, five Eucampia species were identified: Eucampia cornuta, E. groenlandica, E. zodiacus f. zodiacus, E. zodiacus f. cylindrocornis, and E. zodiacus var. cornigera. E. cornuta and E. groenlandica have long pervalvar axis length, but the others display short or moderate length. Ocellus shape of E. cornuta, E. groenlandica and E. zodiacus f. cylindrocornis are linear ribs, whereas E. zodiacus f. zodiacus and E. zodiacus var. cornigera have radial ribs with central area. E. cornuta and E. zodiacus f. cylindrocornis have long and narrow cylindrical elevations. E. groenlandica and E. zodiacus f. zodiacus have short and broad elevations with blunt tips. E. zodiacus var. cornigera has long and broad conical elevations. In terms of aperture shape, E. cornuta has large elliptical form, E. groenlandica has almost circular to rounded rectangular form, E. zodiacus f. zodiacus has narrow and elliptical rounded rectangular to a narrow lanceolate form, E. zodiacus f. cylindrocornis has almost rectangular form, and E. zodiacus var. cornigera has rounded rhombic form. On the basis of elevations in broad girdle view, 5 Eucampia taxa could be divided into 3 types: 'narrow H type', E. cornuta and E. groenlandica; 'regular H type', E. zodiacus f. cylindrocornis and E. zodiacus var. cornigera (partial); 'wide H type', E. zodiacus f. zodiacus (almost).