• 제목/요약/키워드: H-ras

검색결과 173건 처리시간 0.026초

Prediction Model for the Cellular Immortalization and Transformation Potentials of Cell Substrates

  • Lee, Min-Su;Matthews Clayton A.;Chae Min-Ju;Choi, Jung-Yun;Sohn Yeo-Won;Kim, Min-Jung;Lee, Su-Jae;Park, Woong-Yang
    • Genomics & Informatics
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    • 제4권4호
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    • pp.161-166
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    • 2006

  • The establishment of DNA microarray technology has enabled high-throughput analysis and molecular profiling of various types of cancers. By using the gene expression data from microarray analysis we are able to investigate diagnostic applications at the molecular level. The most important step in the application of microarray technology to cancer diagnostics is the selection of specific markers from gene expression profiles. In order to select markers of Immortalization and transformation we used c-myc and $H-ras^{V12}$ oncogene-transfected NIH3T3 cells as our model system. We have identified 8751 differentially expressed genes in the immortalization/transformation model by multivariate permutation F-test (95% confidence, FDR<0.01). Using the support vector machine algorithm, we selected 13 discriminative genes which could be used to predict immortalization and transformation with perfect accuracy. We assayed $H-ras^{V12}$-transfected 'transformed' cells to validate our immortalization/transformation dassification system. The selected molecular markers generated valuable additional information for tumor diagnosis, prognosis and therapy development.

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교각의 세굴심도 최소화를 위한 발파공법 연구 (A Study on Blasting Method for the Smallest of the Scour Depth after Pier Construction)

  • 김가현;김종주;안명석
    • 화약ㆍ발파
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    • 제21권3호
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    • pp.23-35
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    • 2003

  • 본 연구는 측벽 흐름과 배수효과에 의한 홍수시의 이론적인 확산 모델을 확립시켰으며, 기본적인 확산등식은 평균깊이 (H+h)에 관해서 선형화 시켰고 측벽 흐름과 홍수에 의한 배수효과에 관해서는 경계이론을 사용하여 해결하였다. 교각주위의 세굴현상은 교각의 지지 함수와 안정성에 대하여는 교각설치의 기하학적 형상에 따른 하상 바닥 상태와 흐름의 특성에 의존하는 복잡한 문제가 세굴에 영향을 미친다. 그러므로, 교량의 교각에 대한 세굴 깊이를 신뢰하고 평가할 수 있는 통합된 이론이 없으므로 상류 쪽 흐름에 대한 공학적인 판단, HEC-RAS모델적용, 수중발파공법의 연구등 여러가지 방법을 사용하여 세굴관리를 해석할 필요가 있다. 또한 이들은 댐의 건설, 초지 나지, 흐름 등고선, 경작지, 초목지의 조성으로 구성되어져 있다. 높은 제방의 축조를 위해서는 지반을 절취하고, 옹벽 구조물에 의하거나 식생에 의해 하천 제방의 안정을 유지하고 있으며, 저수지 가까이에 적용할 수 있는 재조림 사업으로 초목망의 식물 배양에 의해 이루어지고 있다. 저수지의 상부의 일반적인 하천 바닥에 폭우의 유입시 실트질에 의한 유속감소로 범람이 일어난다. 이러한 작용은 저수지의 주공동에 달하기 전에 광범위한 침식이 일어난다는 것을 알수 있었다

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a-C:H Films Deposited in the Plasma of Surface Spark Discharge at Atmospheric Pressure. Part I: Experimental Investigation

  • Chun, Hui-Gon;K.V. Oskomov;N.S. Sochungov;Lee, Jing-Hyuk;You, Yong-Zoo
    • 한국표면공학회지
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    • 제36권5호
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    • pp.357-363
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    • 2003

  • The aim of this work is the synthesis of a-C:H films from methane gas using surface spark discharge at the atmospheric pressure. Properties of these films have been investigated as functions of energy W delivered per a methane molecule in the discharge. The method enables the coatings to be deposited with high growth rates (up to $100 \mu\textrm{m}$/hour) onto large-area substrates. It is shown that the films consist of spherical granules with diameter of 20∼50 nm formed in the spark channel and then deposited onto the substrate. The best film characteristics such as minimum hydrogen-to-carbon atoms ratio H/C=0.69, maximum hardness $H_{v}$ =3 ㎬, the most dense packing of the granules and highest scratch resistance has been obtained under the condition of highest energy W of 40 eV. The deposited a-C:H coatings were found to be more soft and hydrogenated compared to the diamond-like hydrogenated (a-C:H) films which obtained by traditional plasmaenhanced chemical vapor deposition methods at low pressure (＜10 Torr). Nevertheless, these coatings can be potentially used for scratch protection of soft plastic materials since they are of an order harder than plastics but still transparent (the absorption coefficient is about $10^4$∼$10^{5}$ $m^{-1}$ At the same time the proposed method for fast deposition of a-C:H films makes this process less expensive compared to the conventional techniques. This advantage can widen the application field of. these films substantially.y.

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