• Title/Summary/Keyword: H&E 염색

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Deep Learning Based Digital Staining Method in Fourier Ptychographic Microscopy Image (Fourier Ptychographic Microscopy 영상에서의 딥러닝 기반 디지털 염색 방법 연구)

  • Seok-Min Hwang;Dong-Bum Kim;Yu-Jeong Kim;Yeo-Rin Kim;Jong-Ha Lee
    • Journal of the Institute of Convergence Signal Processing
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    • v.23 no.2
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    • pp.97-106
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    • 2022
  • In this study, H&E staining is necessary to distinguish cells. However, dyeing directly requires a lot of money and time. The purpose is to convert the phase image of unstained cells to the amplitude image of stained cells. Image data taken with FPM was created with Phase image and Amplitude image using Matlab's parameters. Through normalization, a visually identifiable image was obtained. Through normalization, a visually distinguishable image was obtained. Using the GAN algorithm, a Fake Amplitude image similar to the Real Amplitude image was created based on the Phase image, and cells were distinguished by objectification using MASK R-CNN with the Fake Amplitude image As a result of the study, D loss max is 3.3e-1, min is 6.8e-2, G loss max is 6.9e-2, min is 2.9e-2, A loss max is 5.8e-1, min is 1.2e-1, Mask R-CNN max is 1.9e0, and min is 3.2e-1.

The Novel Label Free Staining Algorithm in Digital Pathology (차세대 디지털 병리를 위한 Label Free 디지털염색 알고리즘 비교 연구)

  • Seok-Min Hwang;Yeun-Woo Jung;Dong-Bum Kim;Seung Ah Lee;Nam Hoon Cho;Jong-Ha Lee
    • Journal of the Institute of Convergence Signal Processing
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    • v.24 no.1
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    • pp.76-81
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    • 2023
  • To distinguish cancer cells from normal cells, H&E (Hematoxylin & Eosin) staining is required. Pathological staining requires a lot of money and time. Recently, a digital dyeing method has been introduced to reduce such cost and time. In this paper, we propose a novel digital pathology algorithms. The first algorithm is the Pair method. This method learns the dyed phase image and unstained amplitude image taken by FPM (Fourier Ptychographic Microscopy) and converts it into a dyed amplitude image. The second algorithm is the unpair method. This method use the stained and unstained fluorescence microscopic images for modeling. In this study, digital staining was performed using a generative adversarial network (GAN). From the experimental results, we noticed that both the pair and unpair algorithms shows the excellent performance.

The Dyeing Properties of Woody Fiber Regenerated from Waste MDF by Reactive Dyes (반응성염료에 의한 폐MDF 재생 목질섬유의 염색특성)

  • Ju, Seon-Gyeong;Roh, JeongKwan
    • Journal of the Korean Wood Science and Technology
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    • v.47 no.2
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    • pp.163-177
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    • 2019
  • This study aims to review the relations between the dyeing conditions (i.e., dye concentration, addition amounts of salt and alkali, and dyeing temperature) and dyeing properties and color fastness to light for identifying the optimal dyeing conditions when dyed regenerated woody fibers were obtained through the defibration of waste medium density fiberboard (MDF) using reactive Red H-E3B (Bis-monochlorotriazine (MCT)/MCT type) and reactive Red RB133% (Bis-MCT/Vinyl sulphone type). The dyeing yield (K/S) obtained using two types of reactive dyes increased as the dye concentration increased by 1-10% (on the weight of fiber (OWF)). In addition, the K/S of H-E3B was higher than that of RB133% irrespective of the dye concentration. The color difference of H-E3B after ultraviolet (UV) radiation was lower than that of RB133%, denoting good resistance to discoloration by UV. As the amount of sodium sulfate increased, the color difference and K/S also increased, and the adequate salt content was determined to be 50-70 g/L. Further, the color difference and K/S significantly increased only the addition of 2 g/L of sodium carbonate; however, almost no difference was observed when more than 2 g/L of sodium carbonate was added. The addition amount of sodium carbonate was adequate 5-10 g/L to dyeing the fiber and the pH at this addition level was 10. The dyeing yield of H-E3B increased when the dyeing temperature increased; however, it subsequently decreased after the dyeing temperature became $80^{\circ}C$. The dyeing yield of RB133% was almost the same up to $60-70^{\circ}C$ but declined subsequently. Thus, the adequate temperatures were $80^{\circ}C$ and $60^{\circ}C$ for H-E3B and RB133%, respectively. If the waste MDF woody fiber was dyed under the aforementioned optimal conditions, dyed regenerated woody fiber can be obtained having the following colors: 1.5 to 2.0R with the H-E3B dye and 9.6 to 10.0 PR with RB133%.

Color Characteristics of Silk Fabrics Dyed with Natural Red Dyes Without a Mordant (적색계 천연염료를 이용한 견직물 무매염 염색의 염색성)

  • Kim, Hyo-Jin;Lee, Joo-Hyeon
    • Journal of Digital Convergence
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    • v.16 no.12
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    • pp.593-602
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    • 2018
  • The purpose of this study was to investigate the coloring properties of non-mordant dyes by examining local literature on silk fabric dyeing using red-colored natural dyes. Natural dyes can be prepared from the following 8 materials: purple-fleshed sweet potato, Impatiens balsamina, mulberry, fruits of Ligustrum japonicum Thunb, guava leaves, dansam, hibiscus flowers, and pruned branches of Prunus persica. To examine the surface color calculated $L^*$, $a^*$, $b^*$, ${\Delta}E^*$, and K/S value and H V/C. The variables considered in the dyeing experiment were dye concentration, dyeing temperature, dyeing time, and number of dyeing iterations, which were varied to evaluate the dyeing properties and color characteristics. As a result, the abovementioned variables, dye affinity, and red color expression were directly proportional to one another. In this study, it was found that red coloring can be obtained with natural dyes; moreover, excellent dyeing was achieved without the need for repeated dyeing or mordanting processes, which cause environmental pollution.

땅콩껍질을 이용한 나일론직물의 천연염색

  • Kim, In-Ok;Heo, Man-U
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2008.10a
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    • pp.51-52
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    • 2008
  • 폐기되고 있는 땅콩껍질로부터 재활용이라는 로하스 개념을 적용한 천연염색 자원개발 측면에서 땅콩껍질의 색소 추출을이용하여 나일론직물의 염색성, 염색 견뢰도와 더불어 기능성을 토하고자 하였다. 볶은 땅콩(학명 Arachis hypogaea)의 외피를 분리하여 땅콩껍질 5kg에 증류수 10L을 가하여 $100^{\circ}C$에서 120분간 환류시켜 색소를 추출 여과하였다. 매염제는 시약은 1급을 그대로 사용하였다. 시료는 KS K 0905에 규정된 염색 견뢰도 시험용 정련된 표준 나일론직물 사용 하였으며 염액의 욕비 1:50, 시간에 따른 염색조건, 염색온도, pH, 반복 염색성은 $100^{\circ}C$에서 90분씩 염색하여 건조하는 순서로 6회 반복 염색하였다. 염색온도가 높아질수록 시간이 길어질 수록황색이 점차 진해지며 온도 증가함에 따라 K/S값과 ${\Delta}E$값이 점차 증가함을 보여주고 있다.

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Dyeability and Antibacterial Activity of the Fabrics Using Balsamine Extracts (봉숭아 추출액의 염색성과 염색물의 항균성에 관한 연구)

  • 김재훈;유혜자
    • Textile Coloration and Finishing
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    • v.15 no.1
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    • pp.15-22
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    • 2003
  • Dyeing liquor was extracted from flowers, leaves and stems of balsamine. Dyeing properties, colorfastness and antibacterial activities of the fabrics dyed with the balsamine extracts were investigated at a variety of dyeing conditions of differing pH. time and temperature. Wool, silk and nylon fabrics showed better dyeability than cellulosic fabrics, i.e. cotton, linen and rayon. Especially, nylon fabrics showed the highest K/S values. The dye-uptake was achieved to the highest degree during the first ten minutes of dyeing procedure. All fabrics were dyed well at $40^\circ{C}$ with no pH adjustment. The pH of unadjusted balsamine extracts was 4.6. Their colorfastnesses to washing, drycleaning and perspiration were good as 4 or 4-5 grade. but the light fastnesses were poor as 1-2 grade. While, the antibacterial activities to Staphylococcus aureus and Klebsiella pneumoniae were excellent with 99.9% of colonies reduction ratio. Their excellent antibacterial activity was kept well after ten times washing.

A Study on the Dyeing Properties of Silk Fabrics Dyed with Impatiens balsamina extract (봉선화 추출액을 이용한 견직물 염색)

  • 장재철;김애순
    • Textile Coloration and Finishing
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    • v.15 no.1
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    • pp.1-7
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    • 2003
  • To study the dyeability(ΔE value) and color depth of silk fabrics dyed with Impatiens balsamina extracts. its flower, leaf and stem was extracted by water. This paper investigated some experiments under the various dyeing conditions such as dyeing temperature, dyeing time, repetition of dyeing, dyeing pH with silk fabrics dyed with Impatiens balsamina extracts. The results obtained were as follows 1. It was found that $\lambda_{max}$ of silk fabrics dyed with Impatiens balsamina extract was 260nm and its flower, leaf , stem and different colored flower(white, pint violet) had same $\lambda_{max}$ at 260nm. 2. $\Delta{E}$ values were increased when dyeing temperature, repetition of dyeing and time was higher, value of munsell of silk fabrics dyed with Impatiens balsamina extract was Yellow-Red. But dyeing temperature, repetition of dyeing and time was higher, shifted to longer wavelenth side. 3. Silk fabric dyed with Impatiens balsamina extracts at acidic pH was colored reddish Yellow-Red, alkali bath colored yellowish Yellow-Red in silk fabrics dyed with Impatiens balsamina extract.

A Study on the Dyeing Properties of Silk and Cotton Fabrics with Chestnut Bur Extract(2) (밤송이 추출액을 이용한 견과 면직물 염색 (2))

  • Kim, Ae Sun;Jang, Jae Cheol
    • Textile Coloration and Finishing
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    • v.13 no.2
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    • pp.7-7
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    • 2001
  • This paper investigated the dyeability and surface color of silk and cotton fabrics dyed with chustnut bur extract. Some experiments were performed under the various dyeing conditions such as dyeing temperature, dyeing time, repetition of dyeing, dyeing pH, mordanting condition, washing fastness and light fastness with silk and cotton fabrics. The results obtained were as follows 1. △E value was increased when dyeing temperature and time was higher, value of munsell was shifted from Yellow to Yellow-Red at hither dyeing temperature and time in silk and cotton fabrics. 2. Optimal dyeing pH was increased with acidity of dyeing solution in silk and cotton fabrics, and repetition of dyeing did not change the dye-uptake. 3. When mordanting time and temperature was higher, △E value was larger in silk and cotton fabrics. Silk and cotton fabrics with Fe-premordanting treatment had best dyeability, but treatment with Sn-mordanting was worst. 4. Washing fastness and light fastness was excellent in various dyeing condition at silk fabric but cotton was without practical use.

A Study on the Dyeing Properties of Silk and Cotton Fabrics with Chestnut Bur Extract(2) (밤송이 추출액을 이용한 견과 면직물 염색 (2))

  • 김애순;장재철
    • Textile Coloration and Finishing
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    • v.13 no.2
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    • pp.93-103
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    • 2001
  • This paper investigated the dyeability and surface color of silk and cotton fabrics deed with chustnut bur extract. Some experiments were performed under the various dyeing conditions such as dyeing temperature, dyeing time, repetition of dyeing, dyeing pH, mordanting condition, washing fastness and light fastness with silk and cotton fabrics. The results obtained were as follows 1. $\Delta{E}$ value was increased when dyeing temperature and time was higher, value of munsell was shifted from Yellow to Yellow-Red at hither dyeing temperature and time In silk and cotton fabrics. 2. Optimal dyeing pH was increased with acidity of dyeing solution in silk and cotton fabrics, and repetition of dyeing did not change the dye-uptake. 3. When mordanting time and temperature was higher, $\Delta{E}$ value was larger in silk and cotton fabrics. Silk and cotton fabrics with Fe-premordanting treatment had best dyeability, but treatment with Sn-mordanting was worst. 4. Washing fastness and light fastness was excellent in various dyeing condition at silk fabric but cotton was without practical use.

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The Changes in Properties of Silk Fabrics Dyed with Safflower under Ultraviolet-Light (홍화 염색 견직물의 자외선에 의한 성능 변화 연구)

  • Shin, Youn-Sook;Choi, Seung-Youn
    • Journal of the Korean Home Economics Association
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    • v.46 no.7
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    • pp.1-6
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    • 2008
  • The purpose of this study was to investigate the changes in properties of silk fabrics dyed with safflower red and yellow colorants under Ultraviolet(UV)-light. Silk fabrics dyed with safflower red and yellow colorants were compared with each other after uv-light exposure in terms of K/S value, color changes(${\Delta}E$), morphology, and strength retention. The K/S value rapidly decreased with increasing exposure time for samples of both colorants, but the extent of decrease was less for the samples dyed with safflower yellow colorant, than that of the samples dyed with safflower red colorant. Regarding the color changes with increasing exposure time, $L^*$ and $b^*$ increased, $a^*$ decreased, and thereby ${\Delta}E$ increased in the red-dyed samples, whereas $L^*$ increased, $a^*$ and $b^*$ decreased, and so ${\Delta}E$ increased in the yellow-dyed samples, indicating that the UV-light induced fading and changes of hue, value and chroma value. However, the color change of the yellow-dyed samples was less than that of the red-dyed samples. SEM images showed a severe degradation by UV-exposure for both colorant samples. Tensile strength slowly decreased until 14 days, but rapidly decreased thereafter. Strength retention of the yellow-dyed samples was higher than that of red-dyed samples.