• Journal of Mushroom
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• v.16 no.2
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• pp.70-73
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• 2018

To determine the optimum amount of spent oyster mushroom substrate (SOMS) for use in cultivation of Lentinula edodes, the chemical properties of the substrate and culture conditions of Lentinula edodes were investigated. Replacing 20-50% of a sawdust substrate with SOMS yielded a C/N ratio of 62-76. In case of substrates containing SOMS, the total nitrogen and phenolic contents of were higher, whereas fructose and organic acid contents were lower than those of the control substrate. Cultivation tests showed that the 3-cycle yield of 20% SOMS treatment was 286.7 g, similar to that of the control, while 50% SOMS treatment significantly decreased the yield. In conclusion, development of oak mushroom substrate using SOMS would recycle waste products and decrease material costs.

• Proceedings of the Microbiological Society of Korea Conference
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• 2009.05a
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• pp.52-52
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• 2009

• Journal of Mushroom
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• v.16 no.3
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• pp.225-230
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• 2018

This study was conducted to reuse spent mushroom substrates (SMS) of Pleurotus ostreatus and improve their nitrogen content by bacterial treatment. Two kinds of bacteria were used to investigate the increase in total nitrogen (T-N) content. Bacillus sp. (GM20-4) was isolated from SMS of oyster mushroom, and Rhodobacter sphaeroides (RS) was obtained from Gwangju Si Agricultural Technology Center. SMS samples were collected from three oyster mushroom cultivation farms located in Gyeonggi-do province, Korea. When dried SMS was inoculated with 30% culture broth of GM20-4 and RS and incubated at room temperature ($25{\pm}2^{\circ}C$) for 5 days, T-N content increased. To investigate the T-N content of other SMS, three dried SMS samples (A, B, and C) were treated by the same method using GM20-4 and RS. As a result, the T-N content of sample B was 20% higher than that of the control, whereas the T-N content of samples A and C increased to 17% and 12%, respectively. The change in T-N content by bacterial treatment of wet SMS was slightly higher than that of the control. The changes in amino acid content were also found to be higher than those in the control in all SMS samples by GM20-4 and RS treatment. Aspartic acid and glutamic acid contents were the highest among all amino acid compositions. Especially, the aspartic and glutamic acid contents of sample B increased by 2.9 folds higher than the control.

• Journal of Mushroom
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• v.16 no.4
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• pp.257-262
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• 2018

The aim of this study was to re-use spent mushroom substrate (SMS) with increased total nitrogen (T-N) and amino acid content and reduce the amount of cottonseed meal used as nutrient supplement in Pleurotus ostreatus cultivation. Bacteria used for improvement of the T-N content were GM20-4(Bacillus sp.) and Rhodobacter sphaeroides (RS). GM20-4 was isolated from the SMS of P. ostreatus and RS was obtained from Gwangjusi agricultural technology center. SMS in T1, T2, and T3 was reused as substrate after drying and the T-N content of dried SMS (D-SMS) was increased by 0.34% by treatment with the bacteria. T1 with 8% D-SMS and T2 with 18% D-SMS had higher rates of primordia formation compared with T3 and the control. The biological efficiency of the control and of treatment with 8%, 18%, and 26% D-SMS was 110%, 114%, 112%, and 79%, respectively. Considering the economic cost, yield, and biological efficiency, T2 with 18% D-SMS as the culture substrate for P. ostreatus was shown to be the most effective for cultivation.

• 한국균학회소식:학술대회논문집
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• 2018.05a
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• pp.29-29
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• 2018

Sparassis latifolia is called "Cauliflower Mushroom" and is known as an edible mushroom that has high content of ${\beta}$-glucan. Recently, artificial cultivation of S. latifolia has been done by bottle, plastic bag and wood cultivation in Korea. However it is not widely used because there are low incubation ratio and yield. For the high efficiency of production, we aim to find the superior strains and media for better mycelial and fruit body growth. First, we analyzed the genetic relationship among 31 strains and divided five groups with three kinds of URP primers. And then ten strains were selected from five groups based on the experiment of mycelial growth. The suitability of media for mycelial growth was different according to media type. The suitable solid and liquid media for mycelial growth of S. latifolia isolates were PDA and M2, respectively. In addition, with regard to C/N ratio, the mycelial growth increased even until C/N 160. Second, we investigated the production of fruitbody of the strains by plastic bag cultivation. The substrate was mixed with larch sawdust, corn flour, and wheat flour (8:1:1, v/v). Moisture content of substrate was controlled by about 60% with 10% molasses solution. Out of 31 strains, 19 strains formed primordia. The eight strains produced more than 140g/1kg in fresh weight. Third, molasses culture media was selected for the mycelial growth. And molasses suitable sugar content and input aeration were around 8Brix% and 0.3~0.6vvm, respectively. The longer the incubation period is, the more dried weight of mycelia increased, but medium volume decreased. Therefore, the best incubation period was 9 to 11 days depending on strains. In the future, research project entitled development of culture system and new variety for stable production of S. latifolia will be considered as a new item.

• Journal of Mushroom
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• v.17 no.3
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• pp.107-112
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• 2019

This study was conducted to develop a healthy functional food material using mycelial extract of Phellinus sp. Jangsoo and GMPL48007 were selected as suitable strains because of good mycelial growth and high functionality. The highest total polyphenols and reducing power were observed in the Jangsoo strain and the radical scavenging was shown in GMPL48007. Among grains media, whole-grain wheat had the highest extraction yield from mycelia of selected strains; especially, the GMPL48007 strain showed the highest content of beta-glucan.

• Journal of Mushroom
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• v.18 no.1
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• pp.91-94
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• 2020

This study was carried out to develop a method for cultivation of Lentinula edodes that would reduce both labor and waste. Cultural characteristics were studied, and mushroom yields were estimated to identify the most suitable method to culture L. edodes, using bottles and boxes. Among these, box cultivation with 7 kg of substrate had 40 days of browning period and the maximum yield of 945 g fruiting body; its biological efficiency was 32.7%. In contrast, box cultivation after pre-culture, and the bottle cultivation were found to be unsuitable for the production of L. edodes, due to long periods of browning, and low yields with poor quality, respectively. Further studies on box cultivation of L. edodes are necessary for commercial application of this method.

• Journal of Mushroom
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• v.16 no.2
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• pp.86-89
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• 2018

To develop Grifola frondosa as a new commercial product, we investigated the optimal primordium formation method for bottle cultivation. Primordium formation was initiated on the sixth day in all treatments, but in the T7 treatment, which consisted of removing old spawn and turning the bottle upside-down, was initiated on the ninth day. The ratio of primordium formation was at its highest value, 98.9%, in T3 and T7. Among the treatments, T7 produced the highest quality fruiting bodies and a mushroom yield of 112.8 g in each 850 mL bottle, but its crop cycle time was somewhat longer than those of the other treatments. These results indicate that appropriate methods for primordium formation in bottle culture could enhance mushroom quality and productivity.

• Journal of Mushroom
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• v.18 no.3
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• pp.254-259
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• 2020

Grifola frondosa is a promising new kind of cultivated mushroom owing to its excellent taste and functionality. However, more research is required to determine its value. In this study, the effects of pretreating Grifola frondosa tea by drying, steaming, and warming, on quality characteristics and antioxidant activities were analyzed. There was no difference in sugar, soluble solid, and nitrogen content between dried and steamed tea. The color of the warm pretreated tea was the darkest, and the amino acid content was 462.9 mg/L, which was 1.8 times higher than that of the other pretreatments. Moreover, the warm pretreated tea had the highest total polyphenol and flavonoid content, 14.6 mg/g and 2.2 mg/g, respectively. DPPH and ABTS radical scavenging activities significantly increased with the increase of phenolic compounds. Warming pre-treatment slightly increased the taste preference to 7.0. In conclusion, warming Grifola frondosa before drying was confirmed to improve the extraction of nutrients, antioxidants, and taste preference.

• Journal of Mushroom
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• v.17 no.3
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• pp.132-135
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• 2019

This study aimed to develop a new method of Lentinula edodes cultivation to reduce labor and waste disposal. To identify the optimum container for L. edodes cultivation, box, bottle, and bag containers were assessed for their effects on the cultural characteristics and mushroom yield. Among the three approaches, box-cultivation with 7 kg of substrate had the shortest browning period (42 days) and resulted in the highest mushroom yield (1,253 g). Contrastingly, bottle-cultivation was unsuitable for cultivation due to low production of the fruiting bodies and poor quality. Further studies on the box-cultivation approach will be required for commercial applications of L. edodes.