• Title/Summary/Keyword: Gryllotalpa

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Molecular Cloning of the Sec61p ${\gamma}$ Subunit Homologue Gene from the Mole Cricket, Gryllotalpa orientalis

  • Kim, Iksoo;Lee, Kwang-Sik;Jin, Byung-Rae;Kim, Eun-Sun;Lee, Heui-Sam;Ahn, Mi-Young;Sohn, Hung-Dae;Ryu, Kang-Sun
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.1
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    • pp.73-77
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    • 2002

  • The Sec61 trimeric complex ($\alpha$,$\beta$, and ${\gamma}$ subunits) is one of the Sec-complex responsible for post-translational protein translocation across the endoplasmic reticulum membrane in diverse organisms. In this study, a cDNA encoding the Sec61p ${\gamma}$ subunit homologue was isolated from the cDNA library of the mole cricket, Gryllotalpa orientalis. Sequence analysis of a 442-bp cDNA clone showed it to contain an open reading frame of 68 amino acid residues consisted of 204-bp. The homologues of the gene were found in the GenBank database in a diverse organism including insect, mammals, fungi, and plants. The deduced amino acid sequence of Sec61p ${\gamma}$ subunit homologue of the mole cricket showed the highest homology to the gene of the singly known insect, Drosophila melanogester (93% identity), and the least homology to that of the baker's yeast, Saccharomyces cerevisiae (37.2%). Phylogenetic analysis also confirmed a close relationship between the insect Sec61p ${\gamma}$ subunit homologues of G. orientalis and D. melanogester. Hydropathy analysis of the cricket mole and published other data suggested that the hydrophobic segment close to C-terminus is predicted to be the putative membrane anchor, Multiple alignment of the Sec61p ${\gamma}$ subunit homologue among several organisms showed the presence of several conserved domains including the conserved proline at position 28.

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Mitochondrial DNA Sequence Variation of the Oriental Mole Cricket, Gryllotalpa orientalis (Orthoptera: Gryllotalpidae) in Korea

  • Kim, Ik-Soo;Cha, So-Young;Lee, Sun-Young;Kim, Seong-Ryul;Hwang, Jae-Sam;Li, Jianhong;Han, Yeon-Soo;Jin, Byung-Rae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.14 no.2
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    • pp.107-112
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    • 2007

  • The mole crickets, Gryllotalpa, are insect pest distributed in the world. In Korea, G. orientalis was reported to occur, but previous ecological studies suggested the presence of two ecological types. To test this hypothesis, we sequenced a portion of mitochondrial (mt) genome from 48 G. orientali individuals collected over five Korean localities: Busan, Suwon, Okchon, Wonju, and Gangneung. From the sequence analysis, only two haplotypes were obtained, but the sequence divergence between the two haplotypes was 11 %, suggesting the presence of two distinct genetic groups in Korea. Although the population of Busan, Okchon, Wonju, and Gangneung was identified as a single haplotype, but that of Suwon was occupied by both hapotypes. Considering sequence divergence of other insect species occurring in Korea, the divergence estimate found between the two haplotypes seems to be too large to be considered as identical species. This result may suggest that the two differentiated haplotypes found in this study may reflect the previously reported two ecological types found in Suwon, Korea. To further understand the genetic divergence of the two phylogenetic groups, analysis of more variable regions of G. orientalis genome is required.

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Development of continuous indoor rearing technique of mole cricket, Gryllotalpa orientalis Burmeister (Orthoptera: Gryllotalpidae) (땅강아지(Gryllotalpa orientalis Burmeister)의 실내 누대사육 기술개발)

  • Park, Young-Kyu;Lee, Young-Bo
    • Journal of Sericultural and Entomological Science
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    • v.52 no.2
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    • pp.96-101
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    • 2014

  • The present study was conducted for two years to develop the indoor breeding system of mole cricket, Gryllotalpa orientalis Burmeister. In 2013, the total mean number of progenies was $430.{\pm}20.0$ ($mean{\pm}SE$); $15.3{\pm}25.8$ of 2nd-3rd instars, $28.8{\pm}24.0$ 3rd-5th and $2.4{\pm}2.7$ 6th-8th. In 2014, the rearing yielded similar result to that of 2013; $43.5{\pm}31.0$ in total with $25.8{\pm}25.6$ of 2nd-3rd instars, $23.6{\pm}21.3$ 3rd-5th and $2.9{\pm}2.3$ 6th-8th. The successful breeding rate using indoor cages was 46.9% in 2013 and 50.0% in 2014. In total 147 individuals were successfully reared in an outdoor rearing cage with 25 females released and 78 individuals in an indoor rearing cage with 100 females and 50 males released. 86.4% and 89.7% of the nymphs reared were collected within 25cm depth of the soil in the outdoor and indoor cage, respectively.

  • https://doi.org/10.7852/jses.2014.52.2.96 인용 PDF KSCI

Seasonal Changes in Age Structure and Fecundity of the African Mole Cricket (Gryllotalpa africana) Population in Suwon, Korea (수원지방에서의 땅강아지 개체군 연령 분포의 계절적 변화와 산란수)

  • 김기황
    • Korean journal of applied entomology
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    • v.34 no.1
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    • pp.70-74
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    • 1995

  • A study on seasonal changes in age structure and fecundity of Gryllotalpa africana Palisot de Beauvois was conducted in Suwon from 1991 through 194. It seemed that most of newly hatched nymphs emerge to adults in September and October, so have a life cycle a year, and some overwinter as nymphs and emerge to adults from August, so have a life cycle tow years. The mean number of eggs per cell was $36.00\pm$2.84 for egg cells laid in the laboratory, and $47.68\pm$4.35 for egg cells collected in the field. Mean and range of the numbers of egg cells produced per female were $1.17\pm$0.19 and 0~3.

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