• The Plant Pathology Journal
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• v.37 no.1
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• pp.24-35
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• 2021

Blue mold caused by Penicillium expansum is one of the most significant postharvest diseases of apples. Some microorganisms associated with the surface of ripening apples possess the ability to inhibit the growth of P. expansum. However, the existing literature about their colonization in the stages before ripening is not explored in depth. This study aims to characterize the antagonistic capacity of bacterial populations from five fruit development stages of 'Royal Gala' apples. The results have shown that the density of the bacterial populations decreases throughout the ripening stages of fruit (from 1.0 × 105 to 1.1 × 101 cfu/㎠). A total of 25 bacterial morphotypes (corresponding to five genera identified by 16S RNA) were differentiated in which Bacillus stood out as a predominant genus. In the in vitro antagonism tests, 10 Bacillus strains (40%) inhibited the mycelial growth of P. expansum from 30.1% to 60.1%, while in fruit bioassays, the same strains reduced the fruit rot ranging from 12% to 66%. Moreover, the bacterial strains with antagonistic activity increased in the ripening fruit stage. B. subtilis subsp. spiziennii M24 obtained the highest antagonistic activity (66.9% of rot reduction). The matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis revealed that bacteria with antagonistic activity produce anti-fungal lipopeptides from iturin and fengycin families.

• Journal of the korean academy of Pediatric Dentistry
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• v.35 no.2
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• pp.243-258
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• 2008

The purpose of this study was to investigate the relationship between the stages of calcification of various teeth and skeletal maturity stages among Korean individuals. The study subjects consisted 154 female and 179 male ranging from 7 years to 16 years of age. A total of 333 hand-wrist, cephalo-lateral and panoramic radiographs were obtained and analyzed. The tooth development of the mandibular canines, first, second premolars, and second molars were assessed according to the Dermijian's system. Skeletal maturity stages were determined from hand-wrist radiographs by using the SMI system and cephalo-lateral radiographs by using the CVMS, respectively. The results were as follows. 1. The mean ages of each stage of skeletal maturity were consistently younger in female subjects. 2. There was a high correlation between skeletal maturity of hand-wrist and cervical vertebrae in the both sexes. 3. There was a high correlation between skeletal maturity and dental calcification stage of mandibular canines, first premolar, second premolars, and second molar. 4. The mandibular second molar was tooth showing the highest correlation. 5. Percent distributions of the relationship between calcification stages of individual teeth and stages of skeletal maturity were obtained in both sexes. In summary, this suggests that tooth calcification stages from panoramic radiographs might be clinically useful as a maturity indicator of the pubertal growth period.

• Korean Journal of Environmental Agriculture
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• v.38 no.2
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• pp.96-103
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• 2019

BACKGROUND: This study was conducted to develop selenium (Se)- and germanium (Ge)-enriched rice by foliar spray application of organic or inorganic Se and Ge. METHODS AND RESULTS: The time and frequency of organic or inorganic Se and Ge treatment were performed at the five main growth stages as followings: effective tillering stage (E), maximum tillering stage (M), booting stage (B), heading stage (H), grain filling stage (G). The main treatment plots were consisted of (1) 'once' treatment (at each E, M, B, H, G stage, Se/Ge single apply), (2) 'twice I' (at H + G stages, organic or inorganic Se/Ge apply), (3) 'twice II' (at H + G stages, mixture apply of Se + Ge + pesticide). The organic or inorganic Se treatment concentration was 20 and 40 ppm, and the Ge was 50 and 100 ppm. The Se and Ge contents in rice grain (brown rice and polished rice) were analyzed by inductively coupled plasma (ICP). The highest Se content was noted in brown rice 'twice I' with Se 40 ppm (1394.06) at H + G stages, but the lowest was in 'once' with Se 40 ppm ($367.79{\mu}g{\cdot}kg^{-1}$) at B stage. The highest of Se content in polished rice was found in 'twice I' of Se 40 ppm (1090.25) at H + G stages, but the lowest was in 'once' with Se 40 ppm ($403.53{\mu}g{\cdot}kg^{-1}$) at E stage. On the other hand, The highest of Ge content in brown rice was found in 'twice I' with Ge 100 ppm (398.66) at H + G stages, but the lowest was in 'once' with Ge 100 ppm ($139.64{\mu}g{\cdot}kg^{-1}$) at B stage. The highest of Ge content in polished rice was found in 'twice I' of Ge 100 ppm (300.29) at H + G stages, but the lowest was in 'once' with Ge 100 ppm ($142.24{\mu}g{\cdot}kg^{-1}$) at B stage. CONCLUSION: Se and Ge contents both in brown rice and polished rice treated with organic Se and Ge forms were higher than those of inorganic Se and Ge. Overall results concluded that the supplementation of organic Se and Ge contents in brown and polished rice contents were comparatively higher than the inorganic Se and Ge. This is results also proved that the foliar spray application of organic Se and Ge has positive nutritive effect on the rice for regular consumption.

• Ocean and Polar Research
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• v.42 no.2
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• pp.133-139
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• 2020

The aim of this study is to examine the physiological characteristics of an agarophyte Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N. Norris et Fredericq in the early life stage of tetrasporophytes (2n) and gametophytes (n) to select appropriate seedlings for mariculture. Growth experiments were carried out at the combinations of four temperatures (20, 25, 30, and 35℃) and three light intensity levels (20, 60, and 100 µmol photons m^-2 s^-1) in the two ontogenetic stages: discoid holdfasts and erect sporelings. Holdfast areas and sporeling lengths of tetrasporophytes and gametophytes were estimated after 14 days in culture. Relative growth rates (RGRs) for holdfast areas were 7.08-28.38% day^-1 for tetrasporophytes and 11.58-23.67% day^-1 for gametophytes. At 35℃, holdfasts of tetrasporophytes survived with RGRs of 7.08-23.28% day^-1 but those of gametophytes died. Maximal holdfast growth of tetrasporophytes occurred at 30℃ and 100 µmol photons m^-2 s^-1, which were different from gametophytes (25℃ and 100 µmol photons m^-2 s^-1). RGRs of tetrasporophytic sporelings were 2.93-11.11% day-1 and were between 0.78-10.82% day-1 for gametophytes. Maximal growth of A. vermiculophyllum sporelings occurred at 25℃ and 60 µmol photons m^-2 s^-1 for tetrasporophytes, and at 20℃ and 100 µmol photons m^-2 s^-1 for gametophytes. In conclusion, the present results indicate that carpospores could be used as resources of spore-seedling methods having genetic diversity for mass field cultivation because tetrasporophytes showed higher-temperature tolerance and faster-growing ability than gametophytes of A. vermiculophyllum in the discoid holdfast and sporeling stages.

• Korean Journal of Medicinal Crop Science
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• v.12 no.3
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• pp.231-236
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• 2004

Crop productivity would be determined by relative capacity of source and sink. The study was carried out to measure the effects of shading and flower bud pinching on growth and yield of Atractylodes japonica Koidz. Shading treatment after transplanting was done at 100%, 65, 45 or 25% level of natural light intensity but the pinching treatment was to remove all the inflorescences formed after June 10, July 10, August 10 or none. Growth and yield at early and late stages were periodically measured. At the early stage, shading mainly influenced fresh weight rather than morphological characters. At the late stage, however, severer shading decreased numbers of stems, leaves, roots, fraction and total fresh weight. 65% shading from July 1 to September 30 after skipped the emerging and early growth stages showed nearly same growth and morphological characters but more rhizome yield per unit area than non-shading. In flower bud pinching treatment, earlier pinching increased number of roots, fraction fresh weights per plant and rhizome yield per unit area.

• Current Research on Agriculture and Life Sciences
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• v.21
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• pp.17-21
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• 2003

This study aimed to identify leaf sheath function through the investigation of effect of removal times and removed number of leaf sheath on growth and flowering in gladiolus. The leaf sheath influenced on the growth and flowering regardless of light condition at early growth stages. When total leaf sheath were removed in early growth stages, flower stalk length and spike length remarkably decreased. Whereas days of flowering were little different by leaf sheath removal. Also when removed numbers of leaf sheath were 1, 2 and 3, the growth and flowering were little different compared to control. But when removed numbers of leaf sheath were 4, flower stalk length decreased above 25cm compared to others treatment, spike length and number of flower decreased and stem diameter became thick abnormally.

• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.281-286
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• 2007

Gibberellins are growth hormones that play a pivotal role in the growth and development of plants. Present investigations were carried to check the effect of nitrogen(N) and silicon(Si) on bioactive $GA_1$ and its immediate precursor $GA_{20}$ at different growth stages of two rice cultivars with different maturity traits. It was observed that the endogenous bioactive $GA_1$ level gradually increased during vegetative stage and anthesis stage of both Junghwabyeo(early flowering cultivar) and Daesanbyeo(late flowering cultivar). However, the $GA_1$ and $GA_{20}$ content start decreasing during the seed filling stage in both rice cultivars, which indicated a possible relationship of bioactive $GA_1$ and floral development. Our results also confirmed that early 13-hydroxylation pathway was operated at all developmental stages of rice plant. Variation in the levels of the endogenous gibberellins in rice shoots were measured by GCMS-SIM using $^2H_2$-labeled gibberellins as internal standards. Combined application of N and Si enhanced growth parameters and reduced lodging index of both rice cultivars. It was thus concluded that the level of physiologically active $GA_1$ increased during vegetative and early reproductive stage, but starts declining at seed filling stage.

• Journal of The Korean Society of Grassland and Forage Science
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• v.8 no.2
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• pp.110-116
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• 1988

The response of forage yield was studied with various growth indices to develop yield model and to determine optimum cutting time in three cultivars of orchardgrass. 1. Number of tiller per plant was the highest at 3rd cutting stage. But, it was decreased rapidly at 4th cutting stage. Leaf Area Index (LAI) was the highest at 3rd cutting stage. LAI was increased slowly during 15 days to 20 days after cutting and thereafter increased rapidly. 2. In dry matter yield over cutting stages, 1st cutting and 3rd cutting stages were higher yield than others. Change of dry matter yield was similar to that of LAI in all cutting stages. 3. Leaf Elongation Rate (LER) and Specific Leaf Weight (SLW) were reached to maximum at 20 to 25 days and 25 to 30 days after cutting, respectively. 4. Dry matte yield was highly correlated with LAI (r-0.905)and with CGR (r-0.962) over three cultivars. Also, LAI was significantly with LER. The best-fit yield model was obtained in multiple regression equation which included both dependent variables of LAI and CGR. 5. Optimum cutting times which were determined by the relationships between D.M. yield and LAI, and between D.M. yield and CGR, were ranged from 32 days to 36 days depend on each cutting stages.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.5
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• pp.425-431
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• 1993

Temperature effects on the grain filling of winter barley(CV. Gangbori, Olbori and Suwon 18) were studied in controlled environments under three day /night temperature regimes (viz. 25/20, 20/15 and 15/10$^{\circ}C ) and at three stages of development, viz. Vegetative, spike development and grain filling stages. Physiological maturity times to the temperature regimes were observed at 53 to 57 days after heading at 15/10$^{\circ}C , 43 to 45 days and 32 to 33 days at 20/15$^{\circ}C and 25/5$0^{\circ}C$, respectively. When plants were grown in 25/20$^{\circ}C during the spike development stages, the number of spikes per plant and grains per spike decreased by 39% and 41% respectively compared with 20/15$^{\circ}C of day / night temperature. The effect of high temperature on grain weight was not significant in all growth phases except post-anthesis where grain weight decreased by 30%. Therefore, the most important temperature effects were round during the spike development stages.

• Journal of The Korean Society of Grassland and Forage Science
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• v.8 no.3
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• pp.110-116
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• 1988

The response of forage yield was studied with various growth indices to develop yield model and to determine optimum cutting time in three cultivars of orchardgrass 1. Number of tiller per plant was the highest at 3rd cutting stage. But, it was decreased rapidly at 4th cutting stage. Leaf Area Index (LAI) was the highest at 3rd cutting stage. LA1 was increased slowly during 15 days to 20 days after cutting and thereafter increased rapidly. 2. In dry matter yield over cutting stages, 1st cutting and 3rd cutting stages were higher yield than others. Change of dry matter yield was similar to that of LA1 in all cutting stages. 3. Leaf Elongation Rate (LER) and Specific Leaf Weight (SLW) were reached to maximum at 20 to 25 days and 25 to 30 days after cutting, respectively, 4. Dry matter yield was highly correlated with LA1 (r=0.905)and with CGR (r=0.962) over three cultivars. Also, LA1 was significantly with LER. The best-fit yield model was obtained in multiple regression equation which included both dependent variables of LA1 and CGR. 5. Optimum cutting times which were determined by the relationships between D.M. yield and LAI, and between D.M. yield and CGR, were ranged from 32 days t o 36 days depend on each cutting stages.