• Title/Summary/Keyword: Growth phase

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Growth and Cadmium Removal in Recombinant Saccharomyces cerevisiae Harboring A Metallothionein Gene (Metallothionein 유전자가 도입된 재조합 Saccharomyces cerevisiae의 생육과 카드뮴 제거)

  • 김대옥;박성식서진호
    • KSBB Journal
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    • v.11 no.5
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    • pp.543-549
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    • 1996
  • Recombinant Saccharomyces cerevisiae BZ-pJ containing the gene coding for metallothionein, a metalbinding protein was grown in the medium with high cadmium concentrations to study the characteristics of growth and cadmium uptake. High concentrations of cadmium reduced cell growth and final cell density and increased the lag phase periods of the recombinant yeast. Addition of 10 mg $Cd^{2+}$/L to the growth medium remarkably decreased a lag period and enhanced the specific cadmium uptake to 52.6 mg $Cd^{2+}$/g dry cell. The effect of copper addition was further investigated in the medium of 680 mg Cd2+/L. An increase in copper concentration from 11.0 to 33.3 mg/L enhanced the specific cadmium uptake from 17.0 to 42.0 mg Cd2+/g dry cell.

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Understanding of Growth Habits of $VO_2$ Film on Graphene and Their Effects on Metal to Insulator $Transition_2$

  • Yang, Jae-Hoon;Kim, Keun-Soo;Jang, A-Rang;Yang, Hyoung-Woo;Kang, Dae-Joon
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.02a
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    • pp.572-572
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    • 2012
  • Growth of metal oxides on graphene may lead to a better understanding of delicate effects of their growth habits on their underlying physics. The vanadium dioxide ($VO_2$) is well known for its metal-to-insulator transition accompanied by a reversible first order structural phase transition at 340 K. This transition makes $VO_2$ a potentially useful material for applications in electrical and optical devices. We report a successful growth of $VO_2$ nanostructures on a graphene substrate via a vapor-solid transport route. As-grown $VO_2$ nanostructures on graphene were systematically characterized by field emission scanning electron microscopy, x-ray diffraction, Raman spectroscopy, FT-IR spectroscopy and high resolution transmission electron microscopy. These results indicate that the strain between $VO_2$ and graphene layers may be easily controlled by the number of underlying graphene layer. We also found that the strain in-between $VO_2$ and graphene layer affected its metal-to-insulator transition characteristics. This study demonstrates a new way for synthesizing $VO_2$ in a desired phase on the transparent conducting graphene substrate and an easy pathway for controlling metal-to-insulator phase transition via strain.

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Effect of different phase levels of medium chain triglycerides on the growth performance, excreta microflora and blood profiles of broilers

  • Hu, Jing;Park, Jae Hong;Zhang, Jian Ying;Yoo, Jong Sang;Cheong, Jin Young;Kim, In Ho
    • Korean Journal of Agricultural Science
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    • v.45 no.2
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    • pp.204-210
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    • 2018
  • Medium chain triglycerides (MCTs) provide polka dot grouper Chromileptes altevelis with a more readily utilizable source of energy than long-chain fatty acids (LCFAs) and significantly elevate the plasma cholesterol level of chickens. As a possible alternative to feed antibiotics, this study investigated the effect of different phase levels of medium chain triglycerides on the growth performance, excreta microflora and blood profiles in broilers. A total of 450 ROSS 308 mixed-sex broilers with an average initial body weight of $49{\pm}0.79g$ (1 day of age) were used in this trial. They were randomly assigned to the following 3 treatments (15 birds / 10 replications): CON (Basal diet); MCT1 (Starter, Grower, Finisher: Basal diet + 0.1%, 0.075%, 0.05% of MCT) and MCT2 (Starter, Grower, Finisher: Basal diet + 0.1%, 0.1%, 0.1% of MCT). The results show that supplementing the diets with different phase levels of MCT did not have a significant impact on the body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR) during the overall experimental period. Additionally, there were no differences in the blood profiles and excreta microflora among the treatments. However, this study found that the BWG was increased by 2.3%, and the FCR was decreased by 0.6% in the broiler fed MCT1 treatment group during overall experiment phase. Therefore, the results suggest that MCTs could be used as an alternative to growth promoting feed additives. Moreover, further research should be done to evaluate the effect of the different levels of MCTs.

Growth and characterization of a Bi-Sr-Ca-Cu-O phase by crystal pulling method (Crystal pulling법에 의한 Bi-Sr-Ca-Cu-O계의 결정 성장과 특성 평가)

  • Yoon, D.H.;Sato, N.;Yoshimoto, N.;Yoshizawa, M.
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.7 no.1
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    • pp.1-5
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    • 1997
  • The $Bi_2Sr_2Ca_{n-1}Cu_nOy$(BSCCO) phase is well known to be a superconductor having a strong anisotropic behavior. It can be seen that it is difficult to control the growth direction. In this study, we try to grow a Bi-Sr-Ca-Cu-O phase crystal by the crystal pulling method with a seed crystal and crucibel rotation. Relatively large crystals of the order of $5{\times}5{\times}5{\textrm}{mm}^3$ dimensions can be obtained. We also discuss the possible crystallization field of the $BiO_{1.5}$-(Sr, Ca)O-CuO ternary phase diagram, and present some results of the characterization and magnetic measurements on the grown crystal.

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Effects of Lactobacillus reuteri-based Direct-fed Microbial Supplementation for Growing-Finishing Pigs

  • Shon, K.S.;Hong, J.W.;Kwon, O.S.;Min, B.J.;Lee, W.B.;Kim, I.H.;Park, Y.H.;Lee, I.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.3
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    • pp.370-374
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    • 2005
  • Two experiments were conducted to evaluate the effects of direct-fed microbial supplementation on the growth performance and nutrient digestibility in pigs. In experiment 1, forty eight pigs were used in a 42-d growth assay. There were four pigs per pen and three pens per treatment. Dietary treatments included 1) NC (without antibiotic basal diet), 2) PC (NC diet+0.1% antibiotic, 100 g/kg chlortetracycline), 3) DFM-1 (NC diet+0.2% Lactobacillus reuteri and Lactobacillus salivarius complex) and 4) DFM-2 (NC diet+0.2% Lactobacillus reuteri, Lactobacillus salivarius, Lactobacillus plantarum and Yeast complex). For the overall period, no treatment had significant effects in growth performance. In the nutrition digestibility, the pigs fed DFM diets were improved in DM and N digestibility compared with the pigs fed NC and PC diets but it was not significantly different. In experiment 2, sixty four crossbred pigs were used in a 98-d growth assay. There were four pigs per pen and four pens per treatment. Dietary treatments included 1) HND (high nutrient diet), 2) LND (low nutrient diet), 3) HND+DFM (HND diet+0.2% Lactobacillus reuteri and Lactobacillus plantarum complex) and 4) LND+DFM (LND diet+0.2% Lactobacillus reuteri and Lactobacillus plantarum complex). For overall period of growing phase, the pigs fed LND diets had improved gain/feed (p=0.01) and for overall period in the finishing phase, the pigs fed LND with DFM diets had higher ADG, ADFI and gain/feed than the others but there were no significant differences (p>0.13). In total period of growing-finishing phase, the pigs fed LND diet had higher gain/feed than the pigs fed HND diets (p<0.05). In growing phase, there were not significant differences among the treatments means for DM and N digestibility. However, the pigs fed diets with DFM had improved N digestibility (p<0.02) compared to the pigs fed diets without DFM in finishing phase. In conclusion, DFM slightly improved the growth performance in growing-finishing pigs.

Monte Carlo Simulation of Densification during Liquid-Phase Sintering

  • Lee, Jae Wook
    • Journal of the Korean Ceramic Society
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    • v.53 no.3
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    • pp.288-294
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    • 2016
  • The densification process during liquid-phase sintering was simulated by Monte Carlo simulation. The Potts model, which had been applied to coarsening during liquid-phase sintering, was modified to include vapor particles. The results of two- and threedimensional simulations showed a temporal decrease in porosity, in other words, densification, and an increase in the average size of pores. The results also showed growth of solid grains and the effect of wetting angle on microstructure.

The Influence of Polytypism and Impurities on the Phase Transformation of Kaolins: II. Halloysite (다중구조형 차이와 불순물이 카올린광물의 상전이에 미치는 영향: II. 할로이사이트)

  • 이수정
    • Economic and Environmental Geology
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    • v.32 no.4
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    • pp.411-418
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    • 1999
  • Mullitization of halloysite was examined with special attention to the influence of polytypism and impurities on the phase transformation. The phase transformation sequence in halloysite is identical with those in kaolinite and in dickite. Difference in the phase transformation above $1000^{\circ}C$ in kaolin minerals is attributed to the different crystal structures of metakaolins or the effect of impurities. Growth of mullite crytals in the discordered kaolinte including halloysite or illite occurs at about $100^{\circ}C$ lower temperature. Cristobalite is also hardly produced.

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Inhibitory Effects of Transforming Growth Factor and Drynariae Rhizoma on Leukocytosis Associated with the Chronic Phase of Arthritis in Mice

  • Bae Sung-Min;Jeong Ji-Cheon
    • The Journal of Korean Medicine
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    • v.25 no.4
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    • pp.26-35
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    • 2004
  • Drynariae Rhizoma (DR), an herbal medicine known to clean blood and improve its circulation, frequently appears as the main ingredient in the prescriptions for bone injuries. Currently, it is unclear how contributes pharmacologically to the reformation of bone. Therefore, we have done this study. Systematic administrations of TGFβ1 and water extract of DR diminished the polyarthritis development in rats. TGFβ administration (0.1-2 ㎍/animal) and DR (10-100 ㎍/animal), initiated 1 day before an arthritogenic dose of streptococcal cell wall fragments, virtually eliminated the joint swelling and distortion observed during the acute phase and the chronic phase of the disease. The TGFβ and DR synergistically suppressed the arthritis when the administration was begun after the acute phase of arthritis. Also, the synergistic activity between TGFβ and DR was confirmed in their suppression of arthritis in rats. Consistent with the inhibition of inflammatory cell recruitment into the synovium, TGFβ1 and DR reversed the leukocytosis associated with the chronic phase of the arthritis.

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A study on the $YBa_{2}Cu_{3}O_{x}$ phase deposition by liquid aerosol PECVD (미립액상 분말에 의한 $YBa_{2}Cu_{3}O_{x}$ 초전도체의 PECVD 증착법)

  • 정용선;오근호
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.6 no.2
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    • pp.229-237
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    • 1996
  • The superconducting phase, $YBa_{2}Cu_{3}O_{x}$ (YBCO), was in-situ deposited on the single crystal MgO substrates, using an aerosol decomposition process in a cold plasma reactor. The solubility and decomposition temperature of the chemical precursors, and the vapor pressures of the solvents, were determined to be the factors crucial to achieving a stoichiometric, crystalline YBCO phase. The deposition parameters for the YBCO phase were 0.3 to 2.7 kPa for the oxygen partial pressure and $800^{\circ}C$ to $940^{\circ}C$ for the substrate temperature. The optimum deposition conditions for the YBCO phase were observed along the CuO decomposition line.

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Development Behavior of Vaporizing Sprays from a High-Pressure Swirl Injector Using Exciplex Fluorescence Method

  • Choi, Dong-Seok;Kim, Duck-Jool;Hwang, Soon-Chul
    • Journal of Mechanical Science and Technology
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    • v.14 no.10
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    • pp.1143-1150
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    • 2000
  • The effects of ambient conditions on vaporizing sprays from a high-pressure swirl injector were investigated by an exciplex fluorescence method. Dopants used were 2% fluorobenzene and 9% DEMA (diethyl-methyl-amine) in 89% solution of hexane by volume. In order to examine the behavior of liquid and vapor phases inside of vaporizing sprays, ambient temperatures and pressures similar to engine atmospheres were set. It was found that the ambient pressure had a significant effect on the axial growth of spray, while ambient temperature had a great influence on the radial growth. The spatial distribution of vapor phase at temperatures above 473K became wider than that of liquid phase after half of injection duration. From the analysis of the area ratio for each phase, the middle part (region II) in the divided region was the region which liquid and vapor phases intersect. For liquid phase, fluorescence-intensity ratio was greatly changed at lms after the start of injection. However, the ratio of vapor phase was nearly uniform in each divided region throughout the injection.

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