• Korean Journal of Food Science and Technology
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• 제49권6호
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• pp.632-637
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• 2017

This study was conducted to determine the minimal processing characteristics of four vegetables (brown mustard, kale, red beet and red lettuce) for wrapping (Ssam). Prior to evaluation, the vegetable leaves were cut, washed, and packaged. The texture, weight loss, browning index, pH, gas concentrations ($O_2$ and $CO_2$), microbial growth, and organoleptic qualities of the leaves were assessed at $10^{\circ}C$ for 7 days. The cutting strength of red beet was significantly different among leaves. The initial springiness of brown mustard was the lowest (0.98%). The weight loss varied between 0.24 to 4.77%, pH ranged from 6.00 to 6.95, and browning index increased to 0.95 during storage. Changes of gas composition were lower in cut leaf than whole leaf. Aerobic bacteria (4.9-8.0 log CFU/g), yeast (not detected), mold (-3.5 log CFU/g), and total coliforms (5.0-8.0 log CFU/g) were assessed in the samples. Thus, overall quality of kale and red beet leaves with minimal processing showed satisfactory results when stored for 7 days.

• Korean Journal of Ecology and Environment
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• 제47권1호
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• pp.32-40
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• 2014

This study was conducted to evaluate the growth response of economical six leafy vegetables that are crown daisy, pak-choi and four kinds of lettuce (Red leaf lettuce, Green leaf lettuce, Head lettuce, Romaine lettuce) by light treatment of LED in plant factory. The light treatments were composed of red, blue, red+farred, red+blue, red+blue+white LEDs, irradiation time ratio of the red and blue LED per minute (1 : 1, 2 : 1, 5 : 1, 10 : 1), and duty ratio of mixed light (100%, 99%, 97%). The following results were obtained in different LED light sources treatments: Shoot biomass and S/R ratio of romaine lettuce were the highest under mixed red+blue LEDs. S/R ratio of head lettuce was higher under mixed red+blue+white LEDs than red+blue LEDs. The others showed no difference in LED light treatment. Shoot biomass, total biomass and S/R ratio of green lettuce, head lettuce and pak-choi were highest in the higher red ratio (5 : 1) on irradiation time of red : blue LED ratios. By the different duty ratio (red+blue and red+blue+white LEDs), Under the mixed light of red+blue, shoot and root biomass of crown daisy and romaine lettuce were high in duty ratio of 100% and 99%, and S/R ratio was highest in all the 6 kinds in duty ratio of 97%. All the 6 kinds showed a fine growth state in low duty ratio (97%). Green lettuce, romaine lettuce and pak-choi showed relatively high shoot biomass and total biomass in low duty ratio of 97% under the mixed light of red+blue+white. S/R ratio of romaine lettuce and head lettuce were highest in the duty ratio of 97% with red+blue+white LEDs. Thus, we can cultivate stably without reference to external factors, if we use appropriate light sources and light quality in closed-type plant factory.

• Journal of Bio-Environment Control
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• 제33권3호
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• pp.156-162
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• 2024

This study was conducted to investigate the effect of rooting volume on the productivity of fresh shoots when growing rosemary in multi-layer cultivation. The 10 cm middle cuttings from which the common rosemary (Rosmarinus officinalis L.) apical bud was removed were planted in a 128-hole tray, rooted, and then transplanted into pots of 125, 200, 550, 750, 1,300, and 2,000 mL to determine the growth characteristics and quantity of young shoots of 1-year-old and 2-year-old rosemary. In the case of 1-year-old rosemary, there was no clear difference in initial growth (30 days after transplanting) between treatments in pot size larger than 550 mL, in the case of 2-year-old rosemary, growth tend to be proportional as the pot became larger. The fresh weight of the underground part of 1-year-old and 2-year-old rosemary was the lowest at 6.9 g and 24.4 g, respectively, when surveyed on July 25 in a 550 mL container, and 10.3 g and 24.9 g, respectively, when surveyed on November 24, and there was a difference between treatments in containers of 750 to 2,000 mL. On the other hand, in the case of 1-year-old rosemary, the fresh weight of the above-ground part increased as the pot became larger, but there was no statistical difference above 1,300 mL, and the fresh weight of 2-year-old rosemary was also significantly higher as the pot became larger. The quality of young shoots was the best for 1-year-old rosemary in a pot of 2,000 mL, but for 2-year-old rosemary, there was a difference in quality depending on the season. Shoot productivity per unit pot was highest at 1,300 mL, but when converted to per unit area, the best was observed at 750 mL. Therefore, the most suitable pot size for intensive production through multi-layer cultivation of rosemary young shoots is judged to be 750 mL (12.5 × 11.5 cm).

• Journal of Life Science
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• 제12권3호
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• pp.242-249
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• 2002

The optimal culture conditions in 500$m\ell$ flask suture, 5$\ell$ jar fermenter and 2,000 $\ell$ large stale culture were investigated to maximize the production of antibiotic on Rhizoctonia solani AC4, the causal agent of vegetables damping-off, by the strain Bacillus ehimensis YJ-37. Starch (1.5%) as a carbon source, peptone (0.6%) as a nitrogen source and MgC1$_2$(0.15%) as a metal ion in the medium containing N $a_2$HP $O_4$(0.3%) showed the highest production of the antibiotic(s) in a rotary shake (200 rpm). Optimal initial pH of the culture medium, culture temperature and culture time for the antibiotic(s) production were pH 8.0, 32$^{\circ}C$ and 54hrs, respertively. Under the optimal renditions in flask culture, cell growth and antifungal activity (clear zone size) were 1.42 $\times$ 10$^{8}$ cfu/$m\ell$ (21g-DCW/ $\ell$) and 13.9 mm, respectively. In 5 $\ell$ jar fermenter (medium 3 $\ell$), optimal air flow, agitation speed and culture time for the antibiotic(s) production were 2 vvm, 200 rpm and 48hrs, respectively. Under the optimal conditions in 5 $\ell$ jar fermenter, tell growth and antifungal activity were 2.06 $\times$ 10$^{8}$ cfu/$m\ell$ (30g-DCW/ $\ell$) and 13.4 mm, respectively. Under the culture conditions of air flow (30 vvm) and agitation speed (200 rpm) at 32$^{\circ}C$ for 10 days in 2,000 $\ell$ large scale culture (medium 1,800 $\ell$, pH 8.0), cell growth and antifungal activity were 0.81$\times$10$^{8}$ cfu/$m\ell$ (12g-DCW/ $\ell$) and 8.6 mm, respectively.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.34-34
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• 2014

Filamentous fungi of the genus Colletotrichum (teleomorph, Glomerella) are considered major plant pathogens worldwide. Cereals, legumes, vegetables, and fruit trees may be seriously affected by this pathogen (1). Colletotrichum species cause typical disease symptoms known as anthracnoses, characterized by sunken necrotic tissue, where orange conidial masses are produced. Anthracnose appears in both developing and mature plant tissues (2). We investigated disease occurrence in apple orchards from 2013 to 2014 in northern Gyeongbuk province, Korea. Typical anthracnose with advanced symptoms was observed in all apple orchards studied. Of late, static fruit spot symptoms are being observed in apple orchards. A small lesion, which does not expand further and remains static until the harvesting season, is observed at the beginning of fruit growth period. In our study, static symptoms, together with the typical symptoms, were observed on apples. The isolated fungus was tested for pathogenicity on cv. 'Fuji apple' (fully ripe fruits, unripe fruits, and cross-section of fruits) by inoculating the fruits with a conidial suspension ($10^5$ conidia/ml). In apple inoculated with typical anthracnose fungus, the anthracnose symptoms progressed, and dark lesions with salmon-colored masses of conidia were observed on fruit, which were also soft and sunken. However, in apple inoculated with fungi causing static symptoms, the size of the spots did not increase. Interestingly, the shape and size of the conidia and the shape of the appressoria of both types of fungi were found to be similar. The conidia of the two types of fungi were straight and cylindrical, with an obtuse apex. The culture and morphological characteristics of the conidia were similar to those of C. gloeosporioides (5). The conidia of C. gloeosporioides germinate and form appressoria in response to chemical signals such as host surface wax and the fruitripening hormone ethylene (3). In this study, the spores started to germinate 4 h after incubation with an ethephon suspension. Then, the germ tubes began to swell, and subsequently, differentiation into appressoria with dark thick walls was completed by 8 h. In advanced symptoms, fungal spores of virtually all the appressoria formed primary hyphae within 16 h. However, in the static-symptom fungus spores, no primary hyphae formed by 16 h. The two types of isolates exhibited different growth rates on medium containing apple pectin, Na polypectate, or glucose as the sole carbon. Static-symptom fungi had a >10% reduction in growth (apple pectin, 14.9%; Na polypectate, 27.7%; glucose, 10.4%). The fungal isolates were also genetically characterized by sequencing. ITS regions of rDNA, chitin synthase 1 (CHS1), actin (ACT), and ${\beta}$-tubulin (${\beta}t$) were amplified from isolates using primer pairs ITS 1 and ITS 4 (4), CHS-79F and CHS-354R, ACT-512F and ACT-783R, and T1 and ${\beta}t2$ (5), respectively. The resulting sequences showed 100% identity with sequences of C. gloeosporioides at KC493156, and the sequence of the ${\beta}$t gene showed 100% identity with C. gloeosporioides at JX009557.1. Therefore, sequence data from the four loci studied proves that the isolated pathogen is C. gloeosporioides. We also performed random amplified polymorphic DNA-PCR, which showed clearly differentiated subgroups of C. gloeosporioides genotypes. The clustering of these groups was highly related to the symptom types of the individual strains.

• Journal of the Korea Organic Resources Recycling Association
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• 제29권1호
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• pp.47-57
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• 2021

This study was conducted to evaluate the properties of mixtures of mixed organic fertilizer (MOF) and various organic sources such as livestock manure compost (LMC), dried compost of poultry manure (CPM), dried food waste powder (FWP) and amino acid by-product fertilizer (ABF) and their influences on growth of lettuce and Chinese cabbage. The content of N, P2O5 and K2O of mixture of MOF, LMC and FWP (MLF) was 3.6~3.9%, 2.1~2.2% and 1.3~1.4%, respectively. Lettuce dry weight of MLF3 treatment blending with MOF (60%), LMC (10%) and FWP (30%) was increased by about 29% than that of MOF. The content of N, P2O5 and K2O of mixture of MOF, CPM, FWP and ABF (MCFA) was 4.5~4.7%, 1.7~1.9% and 1.3~1.4%, respectively. Compared to MOF, growth factors of lettuce and Chinese cabbage in the MCFA treatments were not significantly deferent. These results indicated that MLFs and MCFAs, the mixtures of respectively organic sources and MOF, could be applied as orgnic fertilizer in the cultivation of lettuce and Chinese cabbage, and were expected that LMC and ABF might be used as another sources of organic fertilizer.

• Korean Journal of Environmental Agriculture
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• 제16권2호
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• pp.161-165
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• 1997

This experiment were carried out to clarify the effects of several factory wastes on the emergence and seedling growth of five crop species, rice, Chinese cabbage, melon, and tomato. Wastes of three factories treated by several concentrations on the soil in which crop were seeded. In rice seedling experiments, the rice seedlings were treated with factory wastes hydroponically. Factory wastes used in the experiment were obtained from leather, phenol resin, and dye factory. The growth of rice seedlings was inhibited by each factory wastes, but the dry weight of rice seedling was increased by the low concentration below 1/16 dilution of leather factory waste. During 15 days, dry matter accumulation of rice seedlings treated with undiluted factory wastes decreased to 46.0, 51.4, -5.4% of control by treating wastes of phenol resin, leather, and dye factory respectively. The injury of crops by leather factory waste was severe in tomato but slight in barley. Waste of phenol resin factory affects highly both on Chinese cabbage and on melon. When dye factory waste was treated on each crop, all plants died in the treatments of waste solution which diluted to 1/8 of original waste. Tomato and melon were most sensitive crop species to the waste of dye factory. Although the responses of crops to each factory waste were various, the degree of injuries were more higher in vegetables than cereal crops.

• Korean Journal of Soil Science and Fertilizer
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• 제20권2호
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• pp.169-177
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• 1987

Effect of microbiological product manure (Bio-com) on the growth and yield of vegetables and on physico-chemical properties of soil. Leaf lettuce and Garland Chrysanthemum were taken for test plants. A Jungdong (Series) silty loam soil was used for pot experiments. The results are summarized as following: 1. Treatment with Bio-com stimulated plant growth that were increased in the number of leaf, leaf area, plant height and root length both for Leaf lettuce and Garland Cyrysanthemum. 2. Yield increase of 123-1170% was obtained from Bio-com treatments compared to N.P.K.+manure treatment. Application of 2250 kg/10a of Bio-com gave the highest yield. 3. Fertilizer effect of Bio-com appeared much earlier than that of manure. 4. Plant content of $K_2O$ was highest when treated with Bio-com. Plants absorbed considerably more N, $P_2O_5$ and MgO. 5. Content of N, $P_2O_5$ in Garland Chrysanthemum were higher when treated with Bio-com. 6. Content of $P_2O_5$ in soil was at the highest level. Soil pH, content of exchangeable soil Ca and Mg were also high with Bio-com fertilization.

• Korean Journal of Soil Science and Fertilizer
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• 제41권3호
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• pp.177-183
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• 2008

Sodium is known to reduce a plant growth and yields. However, the relationships between physiological response of seedling and salinity stress caused by growing media are not well understood yet. We conducted experiments to investigate change of some parameters including Na, EC, moisture content in media under different air temperature ($15^{\circ}C$, $25^{\circ}C$), and the response of fruit-vegetables such as cucumber, oriental melon on saline conditions originated from horticultural substrate. Volumetric moisture content of media at $15^{\circ}C$ was 70%, but at $25^{\circ}C$ was decreased by 45% within 22 hrs, showing below optimal matric potential, approximately. During reaction time, the increase of Na concentration was significantly greater in saline substrate than in control. The decrease rate of Na concentration according to supplying irrigation water was higher in saline substrate than in control. $CO_2$ assimilation rate and transpiration rate of Korea melon grown in low temperature were decreased with a Na/cation ratio in hydroponic solution. Water saturation deficit was also increased significantly at $15^{\circ}C$ as compare to $25^{\circ}C$. Saline stress during nursery stage induced a reduction of seedling quality, growth and cucumber yield. The results suggest that the relationship between uncontrolled Na uptake of seedling from saline substrate and meteological condition is responsible for saline stress.

• Journal of Bio-Environment Control
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• 제10권3호
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• pp.181-186
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• 2001

During the growth of fruit vegetables such as pepper, cucumber and tomato, there are light deficiency under the plant canopy. This study was conducted to clarify the effect of light diffusion film on the stem growth, canopy, flowering and fruiting of green pepper in greenhouse. The transmittance of total solar radiation into greenhouse under woven and double films were 90% and 75% of polyethylene film. And the transmittance of photosynthetically active radiation into greenhouse under woven and double films were 96% and 81% of polyethylene film. However, the light diffusions under woven, double and polyethylene films were 46%, 31% and 9%, respectively. The plant height under polyethylene film covered greenhouse was 96.9% cm, taller than those under woven and double films by 6.5, 13.9 cm. And the third node length under woven film covered greenhouse was 8.6 cm, shorter than those under double and polyethylene films by 2.5, 5.7 cm. Also the first branch angle under woven film covered greenhouse was 61.0$^{\circ}$, larger than those under double and polyethylene films by 2.3, 10.3$^{\circ}$. But there was no clear difference in the node numbers among the covering materials. The rate of curved and sterile fruit under woven film covered greenhouse was smaller than those under double and polyethylene films by 4.6, 5.5% and 1.2, 3.6%. But the contents of vitamin C showed no difference among the covering materials and plant densities.