• Fisheries and Aquatic Sciences
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• 제2권1호
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• pp.93-97
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• 1999

The promoter region of alginate lyase gene (aly) from Pseudomonas sp. W7 was used for the high expression of gilthead seabream (Sparus aurata) growth hormone (GH) gene in Esherichia coli. PCR product encoding the premature segment of the growth hormone. was cloned to the downstream of aly promoter. GH was overexpressed With 46 ammo acid of alginate lyase as fusion protein. GH was immunoreactive and production of GH was repressed with supplementation of $0.4\%$ glucose into culture media.

• 한국가축번식학회지
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• 제16권3호
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• pp.225-230
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• 1992

Many transgenic mice expressing human growth hormone gene were infertile. To investigate the infertility of these transfenic mice, it was looked into the estrus cycle and sexual behaviour and also tested through in vitro fertilization whether the germ cells of these mice normal or not. The infertile female transgenic mice were mated to the fertile males of ICR strain, but in almost all of them the vaginal plugs were not detected and their estrus cycles by vaginal smear were almost irregular which kept up estrus or diestrus stage. Many male transgenic mice did not have the ability of sexual behaviour. Therefore the viability of germ cells in infertile male transgenic mice was investigated by in vitro fertilization, but the sperm were normally fertilized with the eggs and the transgene of parent was passed on to the progeny. These results consequently suggest that the infertility of transgenic mice experssing human growth hormone gene may be due to the physiological activity of human growth hormone, not germ cells.

• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1334-1337
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• 2004

The present study was conducted to detect polymorphism at growth hormone gene in Karan Fries bulls. A 428 bp fragment of growth hormone gene spanning over $4^{th}$exon, $4^{th}$intron and $5^{th}$ exon was amplified and digested with AluI restriction enzyme to identify polymorphism at this locus. Karan Fries bulls were found to be polymorphic at this locus. Two genotypes LL and LV were identified in Karan Fries with higher allelic frequency for L allele. In Karan Fries males, the average birth weight, 3 months body weight and daily body weight gains of LL homozygotes were significantly higher than that of LV heterozygotes. Genetic distances of KF bulls with respect to genotype along with 3 months body weight and average daily body weight gain forms a single cluster of bulls with LL genotype, while individuals with LV genotype forms three distinct clusters indicating more influence of L allele on growth traits.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1996년도 한국생물과학협회 국제학술대회
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• pp.171.2-171
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• 1996

No Abstract, See Full Text

• 한국가축번식학회지
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• 제27권1호
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• pp.1-7
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• 2003

MT-GHR(Growth hormone receptor)와 MT-IGF-IR(IGF-1 receptor)유전자를 구축하고 micromani-pulator를 이용하여 토끼 수정란에 유전자를 주입하여 형질전환토끼를 생산하였다. 본 연구에서의 형질전환토끼의 생산효율은 약 3%를 나타내었고 Growth Hormone receptor(GHR)를 가진 형질전환 토끼와 IGF-1 receptor(IGF-lR)를 가진 형질전환토끼를 10마리 이상씩 생산하였다. 또한 정상 토끼와 교배시켜 F$_1$ 새끼를 얻어 유전자가 다음세대에도 전달되는 것을 확인하였다. GHR 이나 IGF-1R 형질전환토끼의 성장률은 정상토끼보다는 약15~25% 정도 빠른 경향을 나타냈고 특히 GHR 형질전환토끼의 성장률이 더 높은 것으로 드러나 GHR 및 IGF-lR유전자가 형질전환토끼에서 성장에 영향을 주었다는 것을 확인할 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권2호
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• pp.161-164
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• 2003

The polymorphism of the growth hormone gene in 12 pig breeds (total n=475) was detected by PCR-Apa I-RFLP, and allele A (449 bp, 101 bp and 55 bp) or allele B (316 bp, 133 bp, 101 bp and 55 bp) were observed. In these pig breeds, we found that European pig breeds had high frequencies of allele B, while Chinese native pig breeds had high frequencies of allele A. In addition, the role of porcine GH was investigated in 117 Nanchang White pigs and 361 Large Yorkshire pigs. Eight traits about growth and carcass were recorded for analyzing associations between GH gene polymorphism and performance quantitative traits. In the Nanchang White pigs, no significant difference was observed between different genotypes and different growth and carcass traits. In Large Yorkshire pigs, those with BB genotype had more lean percentage than pigs with AA genotype (p<0.05). Based on these results, we conclude that the GH locus should be further investigated in commercial breeds to determine its suitability for use in marker-assisted selection programmes.

• 한국발생생물학회지:발생과생식
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• 제6권1호
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• pp.31-35
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• 2002

생쥐에서 페르몬으로 알려진 소수성의 분자와 결합하는 major urinary proteins(MUPs)는 부분적으로 성장 호르몬(GH)의 조절을 받는다. 본 연구에서는 MT-GRF로 형질전환되어 성장호르몬이 증가된 생쥐에서 MUP의 발현을 조사하였다. 이 MT-GRF로 형질전환된 생쥐의 소변에서 MUP이 대조군보다 심하게 저하되어 나타났고, 암컷보다 수컷에서 더 저하되어 나타났다. 또한 MT-GRF를 근육에 주사한 생쥐에서도 MUP는 저하되어 나타났다. 부가하여, MT-GRF로 형질전환시킨 생쥐의 소변에서 전기영동상에서 albumin과 동일하게 이동하는 고분자의 단백질이 다량으로 관찰되었는데 신장의 사구체 여과가 손상되었음을 암시하고 있다. 이 혈장 단백질의 손실도 암컷보다 수컷에서 심하게 나타났다. 결과적으로 GRF의 과다생성은 성별에 차별화된 영향을 미치면서 GH로 형질전환된 동물모델에서 관찰되는 MUP의 발현과 사구체 기능의 변화를 동일하게 유발하고 있었다.

• 한국어병학회지
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• 제7권2호
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• pp.95-103
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• 1994

미꾸라지의 성장호르몬 유전자를 분리하기 위하여 미꾸라지의 cDNA library를 준비하였다. total RNA는 미꾸라지의 뇌하수체로부터 얻었으며 oligo (dT)-coupled magnetic bead를 이용하여 total RNA로부터 mRNA를 순수분리하였다. 정제된 mRNA는 cDNA를 합성하기 위한 기질로 사용하였으며, 합성된 cDNA는 EcoRV/Smal으로 절단된 pBlueKS+ plasmid vector에 삽입하였다. 모든 ligation 반응용액을 E. coli, JM109 균주에 형질전환을 유도하였으며 형질전환 효율을 최대화시키기 위하여 전기천공법을 이용하였다. 얻어진 모든 형질전환주들을 DIG로 표지된 Tilapia의 성장호르몬 유전자를 이용하여 고밀도 colony hybridization 에 의하여 검색하였다. 양성반응을 나타내는 10개의 형질전환주를 분리하여 2차 colony hybridization 및 southern hybridization에 의하여 성장호르몬 유전자가 cloning 되었음을 확인하였다. 10 개의 형질전환주 중 하나인 pCGH1을 probe로 사용한 Tilapia 성장 호르몬 유전자의 염기서열과 비교분석하였으며 53.2%의 유사성을 나타냄을 확인하였다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.230.1-230
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• 1998

No Abstract, See Full Text

• BMB Reports
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• 제34권6호
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• pp.502-508
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• 2001

A cDNA, encoding the human growth hormone (hGH), was synthesized based on the known 191 amino acid sequence. Its codon usage was optimized for a high level expression in Escherichia coli. Unique restriction sites were incorporated throughout the gene to facilitate mutagenesis in further studies. To minimize an initiation translation problem, a 624-bp cassette that contained a ribosome binding site and a start codon were fused to the hGH-coding sequence that was flanked between the EcoRI and HindIII sites. The whole fragment was synthesized by an overlapped extension of eight long synthetic oligonucleotides. The four-short duplexes of DNA, which were first formed by annealing and filling-in with a Klenow fragment, were assembled to form a complete hGH gene. The hGH was cloned and expressed successfully using a pET17b plasmid that contained the T7 promoter. Recombinant hGH yielded as much as 20% of the total cellular proteins. However, the majority of the protein was in the form of insoluble inclusion bodies. N-terminal amino acid sequencing also showed that the hGH produced in E. coli contained formyl-methionine. This study provides a useful model for synthesis of the gene of interest and production of recombinant proteins in E. coli.