• Title/Summary/Keyword: Growth gradient

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DNA Heteropolymorphism of Chum Salmon Detected by Denaturing Gradient Gel Electrophoresis and Real Time PCR (Denaturing gradient gel electrophoresis와 real time PCR 방법을 이용한 연어 유전자들의 DNA 이형 다양성 검색)

  • Ham Seung Hub;Lee Suk Keun;Han Hyon Sob;Jin Deuk Hee
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.5
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    • pp.490-496
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    • 2002
  • In order to detect the DNA heteropolymorphism of chum salmon, selected essential genes were examined in different regional chum salmons, i.e., Korean, Japanese and American by denaturing gradient gel electrophoresis (DGGE) and real time PCR methods. From the promoter regions and introns of growth hormone, mtDNA NDI region, D-loop region, IGF-I, histone H3 and MCH2 several representative primer pairs were obtained and employed for the DGGE with the PCR products from the genomic DNAs of the different regional chum salmons. mtDNA NDI, D-loop region and IGE-I genes showed marked heteropolymorphism between Korean and American chum salmons. Intron C of growth hormone also showed a heteropolymorphism between Korean and Japanese chum salmons. Whereas heteropolnnorphism of histone liH and MCH2 genes was detected among in Korean, Japanese and Asnerican chum salmons in the examined region. The real time PCR disclosed the characteristic incremental production of target DNAs dependent on the heteropolymorphic conditions of genomic DNAa of chum salmons, thus the different regional chum salmons could be grouped by the variable incremental curies. Although the DGGE and real time PCR did not produce the identical results in this study, we suggest that the DGGE and real time PCR could be used for the primary screening of the DNA heteropolymorphism of different animal genome.

Effects of the Furnace Temperature on the Growth Behavior of Directionally Solidified Al-Cu-Mg Alloy (Al-Cu-Mg합금의 일방향응고시 로온도에 따른 응고거동변화)

  • Moon, Cheol-Hee
    • Journal of Korea Foundry Society
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    • v.17 no.6
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    • pp.545-551
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    • 1997
  • 14 cm length Al-15Cu-1Mg alloys have been directionally solidified in 3 mm diameter alumina tubes in a furnace moved with a constant velocity V=12 cm/hr under various furnace temperatures of 660, 710 and $760^{\circ}C$. By analysing the evolution of the temperature profiles along the alloy length during the solidification, the growth characteristics such as the position of the solid/liquid interface, the local growth velocity (R) and the temperature gradient at the solid/liquid interface (G) have been determined. The effects of the furnace temperature on the growth behavior have been investigated by the comparison of R and G values for each temperature. Under the furnace temperature of $760^{\circ}C$, steady state growth region was observed for the latter half of the growth period.

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Hydrothermal Growth of $GaPO_{4}$ Single Crystals in HCI Solution

  • Pan-Chae Kim;Shin-Ichi Hirano
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.1 no.1
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    • pp.60-65
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    • 1991
  • The hydrothermal growth of $GaPO_{4}$ Single Crystals was carried out by the horizontal temperature gradient method. The most promising solvents for the crystal growth of $GaPO_{4}$ are $H_{3}PO_{4}$ and HCl solutions. Single crystals have been hydothermally grown at temperatures over the range $210-290^{\circ}C$ in these solutions with seed crystals. The glowth rates in HCl solution were higher than that for comparable conditions in $H_{3}PO_{4}$ solution. Morphologies of crystals grown at temperatures below $200^{\circ}C$ tended to be bounded by small major rhombohedral(10$\bar{1}$1) faces. In the temperature range from 200 to $430^{\circ}C$, the single crystals have morphologies bounded by prism (10$\bar{1}$0), small major rhombohedral(10$\bar{1}$1) and minor rhombohedral(01$\bar{1}$1) faces at the early stage, and grew with well developed basal(0001) faces by increasing the growth temperature.

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A Study on High Temperature Low Cycle Fatigue Crack Growth Modelling by Neural Networks (신경회로망을 이용한 고온 저사이클 피로균열성장 모델링에 관한 연구)

  • Ju, Won-Sik;Jo, Seok-Su
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.20 no.4
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    • pp.2752-2759
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    • 1996
  • This paper presents crack growth analysis approach on the basis of neural networks, a branch of cognitive science to high temperature low cycle fatigue that shows strong nonlinearity in material behavior. As the number of data patterns on crack growth increase, pattern classification occurs well and two point representation scheme with gradient of crack growth curve simulates crack growth rate better than one point representation scheme. Optimal number of learning data exists and excessive number of learning data increases estimated mean error with remarkable learning time J-da/dt relation predicted by neural networks shows that test condition with unlearned data is simulated well within estimated mean error(5%).

The influence of temperature gradient and rotation rate on Bi4Ge3O12 crystal growth by czochralski method (쵸크랄스키법에 의한 $Bi_4Ge_3O_{12}$ 단결정 육성에서 온도구배와 회전속도가 미치는 영향)

  • 배인국;황진명
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.9 no.6
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    • pp.585-589
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    • 1999
  • In order to grow $Bi_4Ge_3O_{12}$ crystals by the Czochralski method equipped with the auto-diameter control system, we used the resistance heater of our own design. We measure the temperature gradients under-arious thermal configurations. The relation between the critical rotation rate corresponding to the flat interface and the temperature gadient was investigated, and the importance of the axial temperature gradient was pointed out. The results from this work were compared with those obtained by other authors when RF heating was used. The optimal conditions for the crystal growth were determined as follows; under $O_2$ atmosphere with the pulling rate fixed at 2 mm/hr, rotation rate changed from 30 to 23 rpm as the crystal growth proceeded, radial and axial temperature gradients were 50 and $40^{\circ}C$/cm near melts respectively, and the composition was chemically stoichiometric.

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Crystal growth from melt in combined heater-magnet modules

  • Rudolph, P.;Czupalla, M.;Dropka, N.;Frank-Rotsch, Ch.;KieBling, F.M.;Klein, O.;Lux, B.;Miller, W.;Rehse, U.;Root, O.
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.19 no.5
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    • pp.215-222
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    • 2009
  • Many concepts of external magnetic field applications in crystal growth processes have been developed to control melt convection, impurity content and growing interface shape. Especially, travelling magnetic fields (TMF) are of certain advantages. However, strong shielding effects appear when the TMF coils are placed outside the growth vessel. To achieve a solution of industrial relevance within the framework of the $KRISTMAG^{(R)}$ project inner heater-magnet modules(HMM) for simultaneous generation of temperature and magnetic field have been developed. At the same time, as the temperature is controlled as usual, e.g. by DC, the characteristics of the magnetic field can be adjusted via frequency, phase shift of the alternating current (AC) and by changing the amplitude via the AC/DC ratio. Global modelling and dummy measurements were used to optimize and validate the HMM configuration and process parameters. GaAs and Ge single crystals with improved parameters were grown in HMM-equipped industrial liquid encapsulated Czochralski (LEC) puller and commercial vertical gradient freeze (VGF) furnace, respectively. The vapour pressure controlled Czochralski (VCz) variant without boric oxide encapsulation was used to study the movement of floating particles by the TMF-driven vortices.

Crystal Growth of Sapphire for GaN Substrates

  • Yu, Y.M.;Jeoung, S.J.;Koh, J.C.;Ryu, B.H.
    • Proceedings of the Korea Association of Crystal Growth Conference
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    • 1998.06a
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    • pp.157-159
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    • 1998
  • Sapphire crystals were grown by Horizontal Bridgman method. The effects of sliding rate (growth rate) of Molybdenum container, growth atmosphere, temperature gradient and orientation of see on crystal qualities were investigated. The size of the crystals grown was up to 150-200 mm in length, 90 mm in width and 25-35 mm in thickness. Crystals grown under the optimum conditions were colorless, transparent and could not be observed and macroscopic defects, such as bubbles, cracks, twins and mosaic structure. With the grown crystals, prototypes of sapphire substrate for blue wafers were characterized. As a result, we can get hight quality of sapphire wafers with c-axis, 1.5 inches in diameters and 0.33 mm in thickess.

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The Theory of Boundary Distribution of the Plant and Wave Character of the Timber Line on Mt. Paektu (식물의 경계분포 이론과 백두산 삼림한계량의 파동성)

  • 장남기;심규철;이현욱;강경미;소금현
    • The Korean Journal of Ecology
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    • v.21 no.5_2
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    • pp.491-499
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    • 1998
  • An investigation was performed to establish the mathematical theories of a vibration for the plant growth and a wave distribution of a plant population on the boundary condition of a limiting factor in the environment. The mathematical theories of the plant growth vibration and wave distribution had been elucidated by the plant growth and the timber line on the middle slope of the west side of Mt. Paektu. The Betula ermaruii composes the timber line on about 2,060 m elevation of sea label, has a growth vibration on the ground surface and takes a wave distribution due to a boundary condition of alpine temperature gradient.

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The Effects of Increased Temperature on Soybean [Glycine max (L.) Merrill] Growth and Seed Yield Responses in Temperature Gradient Chamber (온도구배챔버에서 온도 상승에 따른 콩의 생육과 수량 반응에 미치는 영향)

  • Lee, Yun-Ho;Cho, Hyeoun-Suk;Kim, Jun-Hwan;Sang, Wan-Gyu;Shin, Pyong;Baek, Jae-Kyeong;Seo, Myung-Chul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.2
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    • pp.159-165
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    • 2018
  • The seed yield of summer plants is affected by climate change due to high temperature. High temperature during the reproductive growth period decrease pod, seed weight in soybean. This study was conducted at National Institute of Crop Science (NICS) during the growing season. The objective of this study was to determine the effect of high temperature on growth and seed yield responses of soybean varieties using a temperature gradient chamber (TGC). In 2017, the Daewonkong (DWK), Pungsannamulkong (PSNK), and Deapungkong (DPK) were grown in three TGCs. Four temperature treatments, Ta (near ambient temperature), Ta+1 (ambient temperature+$1^{\circ}C$), $Ta+2^{\circ}C$ (ambient temperature+$2^{\circ}C$), $Ta+3^{\circ}C$ (ambient temperature+$3^{\circ}C$), $Ta+4^{\circ}C$ (ambient temperature+$4^{\circ}C$), were established by dividing the rows along which the temperature gradient was created. In all three cultivars, beginning bloom (R1) delayed at elevated temperature in $Ta+4^{\circ}C$. In addition, the days to beginning of seed fill and maturity were longer under higher temperature. The numbers of pod, 100 seed weight, and seed yield increased at elevated temperature in DWK. In contrast, seed yield components of PSNK and DPK were reduced in $Ta+4^{\circ}C$. The results suggest that 100 seed weight and seed size of soybean was low by increased temperature in $Ta+4^{\circ}C$ of PSNK and DPK.