• Title/Summary/Keyword: Growth conditions

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Physiological Interactions Between the Herbicide Pretilachlor and the Safener Fenclorim on Rice (제초제(除草劑) Pretilachlor와 해독제(害毒劑) Fenclorim의 수도(水稻)에 대한 생리적(生理的) 상호작용(相互作用))

  • Han, S.S.;Hatzios, K.K.
    • Korean Journal of Weed Science
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    • v.10 no.4
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    • pp.328-337
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    • 1990
  • The incividual and combined effects of the chloroacetanilide herbicide pretilachlor and of the safener fenclorim on the growth and selected physiological processes of rice (Oryza sativa L., var 'Lemont')were evaluated under greenhouse and laboratory conditions. Fenclorim applied at rates ranging from 50 to 300 g a.i./ha antagonized the injurious effects caused by 150 to 900 g a.i./ha of pretilachlor on 15-day old wet-sown rice grown under greenhouse conditions. When used rates of 150 g/ha or higher, fenclorim reversed completely the effects of all doses of pretilachlor on rice. When the two compounds were given simultaneously, fenclorim enhanced the uptake of $^{14}C$pretilachlor into rice leaf mesophyll protoplasts measured for 1 hr, indicating that competition for uptake at the protoplast level is not involved in the protective action of this safener. The safener-induced stimulation of pretilachlor uptake was particularly evident when fenclorim was used at concentrations of 10, 20 and $40{\mu}M$. Following 4 hr of incubation, individual treatments with pretilachlor inhibited the in vitro incorporation of radiolabeled precursors into proteins, DNA, and lipids of rice leaf protoplasts only when used at the high concentration of $100{\mu}M$M. Individual treatments with high concentrations (10 or $100{\mu}M$) of the safener fenclorim inhibited the incorporation of radiolabeled precursors into proteins and lipids of rice protoplasts, but had no DNA synthesis. The combined effects of pretilachlor and fenclorim on the incorporation of radiolabeled precursors into these macromolecules of isolated rice mesophyll protoplasts appeared to be additive or slightly synergistic rather than antagonistic. Fenclorim at $1{\mu}M$ antagonized the effects of pretilachlor on total lipids of rice leaf protoplasts. In addition, individual and combined treat-menu with pretilachlor and fenclorim influenced the incoroporation of$^{14}C$acetate into polar lipids, triglycerides and steryl esters of rice leaf protoplas causing a redistribution of carbon in these lipid fractions. However, these effects were not large enough to explain the herbicidal activity of pretilachlor or to account for the protective action of the safener fenclorim. Overall, the uesults of the present study idnicate that the safener fenclorim does not seem to protect rice against pretilachlor injury by antagonizing its effects on protein, DNA, or lipid syntheses.

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Environmental and Biological Effects at Narodo, in the Southern Water of Korea, on Bloom of Ichthyotoxic Dinoflagellates Cochlodinium polykrikoides (유해성 Cochlodinium polykrikoides 최초 발생에 관한 나로도 해역의 환경학적 특성 및 식물플랑크톤 군집 구조)

  • Cho Eun Seob
    • Journal of Life Science
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    • v.15 no.2 s.69
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    • pp.225-230
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    • 2005
  • The aim of this study was to examine the fluctuation in phytoplankton assemblages with regarding to environmental conditions and nutrients, which were surveyed quarterly over the fours seasons (February, May, July, and October). In turn, an understanding of biological effects should provide insights into a wide range of initiated Cochlodinium blooms in Narodo. Sampling was carried out throughout 2001 on the coasts of Busan (St. 1), Yeosu (St. 2), Narodo (St. 3), Kohung (St. 4), and Kwangdo (St. 5). The maximum surface water temperature was recorded in July, and it ranged from 20 to $22^{\circ}C$. Salinity showed no great variation, which maintained itself in the range of 29-34 psu. The maximum surface salinity was recorded in February, which was about 34 psu. The chlorophyll $\alpha$ concentration of the surface water ranged from 0.01 to $1.3\;{\mu}g\;1^{-1}$. The concentrations of $NH_{4}-N $ were persistently high from February to October; in particular, the peak was observed at St. 1 in February and May (0.15 and $0.19\;{\mu}mol\;1^{-1}$, respectively), while it was detected at St. 2 in July and October (0.22 and $2.2\;{\mu}mol\;1^{-1}$ respectively). Similar trends to those for $NH_4-N $ were observed in the concentrations of $NO_{2}-N$ and $NO_{3}-N$. In contrast to nitrogen, a distinct peak of $NO_{4}-P$ at St. 3, 4, and 5 was observed throughout year $(0.01-0.1\;{\mu}mol\;1^{-1}$ except for October. At St. 1 encounter a peak of cell number of 30,000 and $13{\times}10^3$ cells $1^{-1}$, respectively, in July and October. During the period of this study, the majority of the taxa were diatoms. The dinoflagellates were rather abundant after February, in particular at St. 3, 4, and 5 which attained an abundance of $10\~20\%$without marked fluctuation during the period of this study. At St. 3, the highest average cell width, $178.11\;{\mu}m$, was recorded: the highest cell length, $337.72\;{\mu}m$, was measured in July. Consequently, dinoflagellates bloom in July at Narodo influenced by warm water current are not only associated with a desirable development of cell morphometric characteristics, but also with the health growth of C. polykrikoides. During the period of this study, warm water currents caused an increased water temperature in Narodo, but did not change the amount of nutrients.

Evaluation of Stress Thresholds in Crack Development and Corrected Fracture Toughness of KURT Granite under Dry and Saturated Conditions (포화유무에 따른 KURT 화강암의 균열손상 기준 및 수정 파괴인성 측정(Level II Method))

  • Kim, Jin-Seop;Hong, Chang-Ho;Kim, Geon-Young
    • Tunnel and Underground Space
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    • v.30 no.3
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    • pp.256-269
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    • 2020
  • The objective of this study is to evaluate the stress thresholds in crack development and the corrected fracture toughness of KURT granite under dry and saturated conditions. The stress thresholds were identified by calculation of inelastic volumetric strain from an uniaxial compression test. The corrected fracture toughness was estimated by using the Level II method (Chevron Bend specimen), suggested by ISRM (1988), in which non-linear behaviors of rock was taken into account. Average crack initiation stress(σci) and crack damage stress(σcd) under a dry condition were 91.1 MPa and 128.7 MPa. While, average crack initiation stress(σci) and crack damage stress(σcd) under a saturated condition were 58.2 MPa and 68.2 MPa. The crack initiation stress and crack damage stress of saturated ones decreased 36% and 47% respectively compared to those of dry specimens. A decrease in crack damage stress is relatively larger than that of crack initiation stress under a saturated condition. This indicates that the unstable crack growth can be more easily generated because of the saturation effect of water compared to the dry condition. The average corrected fracture toughness of KURT granite was 0.811 MPa·m0.5. While, the fracture toughness of saturated KURT granite(KCB) was 0.620 MPa·m0.5. The corrected fracture toughness of rock in saturated condition decreases by 23.5% compared to that in dry condition. It is found that the resistance to crack propagation decreases under the saturated geological condition.

Methods of Application and Beneficial Effects of Silicate-Coating Rice Seeds (볍씨의 규산코팅방법에 따른 이용특성과 육묘효과)

  • Kang, Yang-Soon;Kim, Wan Joong;Hwang, Duck Sang;Kim, Hee Kyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.1
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    • pp.30-39
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    • 2020
  • A new silicate coating technology was developed which reduces the impact of dust and loosening during seeding compared to existing silicate-coatings (Seed/Si/Zeolite), and therefore can lower the production costs of rice cultivation. In this method, 100 g of rice seed is coated with 18 mL of liquid silicic acid and then dressed with a mixture containing 80 g of dolomite and 5 g of iron. To determine the most effective method of application and ensure that seedlings developed healthily, a series of experiments were carried out. Infected seeds scattered in seedling boxes and pots (soil and hydroponic) were coated dry, without disinfection. In comparison to the seed which were not treated with the silicate-coating, the new seed (A) were 1.84 times heavier in weight, and were also improved in terms of coating strength and coating color. Compared to the seedlings grown from the non-coated seed, those grown from the new silicate-coated seed were of significantly higher quality (weight/length) and had erect, dark greenish leaves, which are ideal plant characteristics. This was most likely due to increased silicate uptake. The symptoms of bakanae disease in the non-coated seed peaked after 38 days to 54.2%, whereas the control value was 68.8% in the new silicate-coated seed (A). In the infected seedlings grown from the new silicate-coated rice seed, subnormal macro-conidia, namely, a sickle shape spore without a septum; a straight oblong shape spore without a septum and with a thick cell wall; and inter-septal necrosis of a normal spore were detected. It is believed that the strong alkalinity of silicic acid have acted as unfavorable conditions for pathogenicity. In seedlings grown from the new silicate coated rice seed under hydroponic conditions without nutrients, normal root activity and growth was maintained without leaf senescence. Therefore, it was possible to reduce the rate of fertilization. In the future, a new silicate-coated rice seed was required for the study of minimal nutrition for anti-aging of seedlings.

Preparation and Properties of the X-ray Storage Phosphors BaFBr1-xIx:Eu2+, Na+ (X-선 저장 BaFBr1-xIx:Eu2+, Na+ 형광체의 제조 및 특성)

  • Cheon, Jong-Kyu;Kim, Sung-Hwan;Kim, Chan-Jung;Kim, Wan;Kang, Hee-Dong;Kim, Do-Sung;Seo, Hyo-Jin;Doh, Sih-Hong
    • Journal of Sensor Science and Technology
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    • v.11 no.1
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    • pp.9-17
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    • 2002
  • The forming conditions of X-ray storage phosrhors $BaFBr_{1-x}I_x:Eu^{2+}$, $Na^+$ have been investigated, and measured the PSL emission spectra and its intensity, fading characteristics and does dependence of the prepared phosphors. These characteristics were compared with those of commercial image plate (ST-III) obtained from Fuji Photo Film Co. The optimal preparing conditions of $BaFBr_{1-x}I_x:Eu^{2+}$, $Na^+$ Phosphor were 0.5 mol% of $EuF_3$, 4.0 mol% of NaF and composition ratio x=0.3, and the sintering temperature were $950^{\circ}C$ in $H_2$ atmosphere. When the composition ratio x was equal to 0, the spectral range of the luminescence of $BaFBr_{1-x}I_x:Eu^{2+}$, $Na^+$ phosphor was $365{\sim}420\;nm$, and its maximum luminescence intensity appeared at 390 nm. When composition ratio x was not equal to 0, the wavelength ranges and peak of the spectra were shifted to the longer wavelength with the growth of composition ratio x. A good linearity was shown between the PSL intensity and X-ray irradiation dose. The phosphor sample with x=0.3 exhibited better fading characteristics than that of other $BaFBr_{1-x}I_x:Eu^{2+}$ phosphor samples, and the fading characteristics of the PSL intensity at room temperature were shown poorer with increasing $I^-$ ion concentration. The lattice constant of the phosphor becomes larger with increasing the $I^-$ ion concentration.

Biological characteristics of Scuticociliate with a polymorphic life cycle of ciliate and cyst isolated from cultured olive flounder, Paralichthys olivaceus (양식넙치, Paralichthys olivaceus에서 분리된 Ciliate와 Cyst의 생활사를 갖는 스쿠티카섬모충의 생물학적 특성)

  • Jin, Chang-Nam;Kang, Hyun-Sil;Park, Geun-Tae;Lee, Je-Hee;Lee, Young-Don;Choi, Woo-Bong;Heo, Moon-Soo
    • Journal of fish pathology
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    • v.18 no.1
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    • pp.19-28
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    • 2005
  • Scuticociliatosis has badly settled one of most damaging diseases during the seedling production process of olive flounder. Paralichthys olivaceus in Korea. We isolated a new type of Scuticociliate from flounder. The parasite metamorphoses to ciliate and cyst phases with each other by environmental changes and survive for a relatively long span. The ciliate was measured average 41.8 ${\mu}m$ in length and 21.0 ${\mu}m$ in width, and cyst was 17.0 ${\mu}m$ and 13.5 ${\mu}m$, respectively. Nutritional condition was determined as a major parameter of metamorphosing between ciliate and cyst stages. The ciliate transforms to a cyst stage because of food shortage, and the cyst returns to a ciliate stage with a favorite environmental condition and shows active growth and reproduction. The ciliate multiplied at the maximal density of $2.9 {\times} 10^5 {m\ell}^{-1}$cells in vitro cultivation at $15 ^{\circ}C$temperature using MS BHI medium and bacterial food sources. The ciliate could be proliferated at a 2.5 to $30 ^ \circ}C$ temperature range, pH 6 to 9, and 1 to 55 ppt salinity. Particularly, it survived over one week at $0 ^{\circ}C$temperature showing a high resistance against unfavorable environmental conditions. And the cyst survived for 320 days in the condition of $5 ^{\circ}C$with no feeding, but its survival period was markedly shortened in higher temperature conditions. The chemotherapeutants (formalin and hydrogen peroxide) were clarified as effective chemicals against the ciliate during in vitro trials, but the effect of therapeutants differed in proportion, depending upon the density and the bathing time of chemical compounds.

Development of Efficient Screening Methods for Resistance of Tomato to Fusarium oxysporum f. sp. lycopersici (토마토 시들음병에 대한 효율적인 저항성 검정법 확립)

  • Park, Myung-Soo;Jeong, Bo-Ram;Jang, Kyoung-Soo;Choi, Yong-Ho;Kim, Jin-Cheol;Choi, Gyung-Ja
    • Horticultural Science & Technology
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    • v.30 no.4
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    • pp.426-431
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    • 2012
  • This study was conducted to establish an efficient screening method for resistant tomato to Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (FOL). The resistance degrees of the six commercial cultivars of tomato to the pathogen were evaluated by dipping roots of the seedlings in spore suspension of five FOL isolates. On the basis of the results, two cultivars (Dotaerangmaster, resistant cultivar to FOL race 1; Supersunload, resistant cultivar to FOL race 2) and two isolates (KACC40043, FOL race 2; TF104, FOL race 3) were selected for system establishment. The disease development of the FOL isolates on the cultivars according to several conditions including root wounding, incubation temperature, inoculum concentration and dipping period of roots in spore suspension was investigated. The resistance of each cultivar to the disease was a race-specific response and hardly affected by the tested conditions except for incubation temperature of $20^{\circ}C$. The optimum temperature for disease development caused by FOL was 25 to $30^{\circ}C$. On the basis of the results, we suggest that an efficient screening method for resistant tomato cultivars to Fusarium wilt is to dip the non-cut roots of tomato seedlings at two-leaf stage in spore suspension of $1{\times}10^7\;conidia{\cdot}mL^{-1}$ for 0.5 hours and transplant the seedling to plastic pot with horticulture nursery media, and then to cultivate the plants in a growth room at $25^{\circ}C$ for 3 weeks with 12 hours light a day.

Temporal and Spatial Variation Analysis of Suspended Solids, Ionic Contents, and Habitat Quality in the Woopo Wetland Watershed (우포늪 수계에서 부유물, 이온농도 및 서식지 특성에 대한 시 ${\cdot}$ 공간적 변이 분석)

  • Bae, Dae-Yeul;Choi, Ji-Woong;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.39 no.4 s.118
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    • pp.498-507
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    • 2006
  • The main objective of present study was to evaluate how seasonal rainfall influenced natural habitat conditions of 10 metric habitat variables along with ionic conditions and suspended solids in the Woopo Wetland during August 2002-July 2003. Largest spatial variabilities in total suspended solids (TSS) occurred during the summer monsoon and the inorganic suspended solids (ISS), expressed as a inorganic proportion of total solids, showed linearly increasing trend from the upstream to downstream. This phenomenon was mainly attributed to counter flow of turbid water from the main Nakdong-River. During the flooding, ISS : TSS ratio showed large increases (92%) in the downstream than the upstream (43%). For this reason, transparency declined (mean=0.13 m, range=0.08-0.21 m) largely in the downstream reach and thus, chlorophyll-a concentration showed low values (range: $4.2-8.6\;{\mu}g\;L^{-1}$), indicating a direct influence on primary productivity or algal growth by inorganic turbidity. In the 2nd survey, ISS averaged 4.0 mg $L^{-1}$ (3.3-4.8 mg $L^{-1}$), thus the ISS decreased by 14 fold, compared to the ISS in the 1st survey during the flooding, while organic suspended solids (OSS) values were greater than those of ISS, indicating a dominance of organic solids. This condition was similar to solid contents in the 3rd survey, but showed a large difference compared to the 4th survey during the growing season. Habitat health assessments, based on 10 metric habitat variables, showed that QHEI values were greatest in the growing season (May) than any other seasons and largest spatial variations occurred in the 2nd survey. Overall, dataset suggest that seasonal episodic flooding during the monsoon may largely contribute nutrient cycling and sediment contents in the Woopo Wetland and Topyung Stream.

Performance Characteristics of Agitated Bed Manure Composting and Ammonia Removal from Composting Using Sawdust Biofiltration System (교반식 축분 퇴비화 및 톱밥 탈취처리 시스템의 퇴비화 암모니아 제거 성능)

  • Hong, J.H.;Park, K.J.
    • Journal of Animal Environmental Science
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    • v.13 no.1
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    • pp.13-20
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    • 2007
  • Sawdust biofiltration is an emerging bio-technology for control of ammonia emissions including compost odors from composting of biological wastes. Although sawdust is widely used as a medium for bulking agent in composting system and for microbial attachment in biofiltration systems, the performance of agitated bed composting and sawdust biofiltration are not well established. A pilot-scale composting of hog manure amended with sawdust and sawdust biofiltration systems for practical operation were investigated using aerated and agitated rectangular reactor with compost turner and sawdust biofilter operated under controlled conditions, each with a working capacity of approximately $40m^3\;and\;4.5m^3$ respectively. These were used to investigate the effect of compost temperature, seed germination rate and the C/N ratio of the compost on ammonia emissions, compost maturity and sawdust biofiltration performance. Temperature profiles showed that the material in three runs had been reached to temperature of 55 to $65^{\circ}C$ and above. The ammonia concentration in the exhaust gas of the sawdust biofilter media was below the maximum average value as 45 ppm. Seed germination rate levels of final compost was maintained from 70 to 93% and EC values of the finished compost varied between 2.8 and 4.8 ds/m, providing adequate conditions for plant growth.

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The Geochemical Characteristics and Environmental Factors on the Marine Shellfish Farm in Namhae-po Tidal Flat of Taean (태안 남해포 갯벌 패류양식해역의 환경특성)

  • Choi, Yoon Seok;Park, Kwang Jae;Yoon, Sang Pil;Chung, Sang Ok;An, Kyoung Ho;Song, Jae Hee
    • The Korean Journal of Malacology
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    • v.29 no.1
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    • pp.51-63
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    • 2013
  • To assess the effect of environmental factors on the sustainability of cultured production shellfish, we investigated the habitat characteristics of tidal flat (Namhae-po in Taean). We measured the physiochemical parameters (temperature, salanity, pH, dissolved oxygen and nutrients) and the geochemical characteristics (chemical oxygen demand, ignition loss, C/N ratio and C/S ratio). Surface sediments were collected from several site of tidal flat to examine the geochemical characteristics of both the benthic environment and heavy metal pollution. The grain size for research area of tidal flat were similar at the ratio of silt and clay in comparison with the other site of it. The C/N ratio was more than 5.0, reflecting the range arising from the mix of marine organism and organic matter. The C/S ratio (about 2.8) showed that survey area had anoxic or sub-anoxic bottom conditions. The enrichment factor (Ef) and index of accumulation rate (Igeo) of the metals showed that those research areas can be classified as heavily polluted, heavily to moderately polluted, or more or less unpolluted, respectively. Adult surf clam (Mactra veneriformis) density was highest at St. 2 (middle part of the Namhae-po), on the other hand, surf clam spat density was highest at St. 3 (lower part of the Namhae-po). Heavy rain, terrigenous suspended clay with fresh water from neighboring agricultural land, and severe high air temperature during summer could be thought as detrimental causes of spat and adult mortality in Namhae-po tidal flat. We suggested that the growth of shellfish in the tidal flat was effected by the various environmental conditions, so an improvement in the cultured method was needed.