• 한국환경보건학회지
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• 제35권5호
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• pp.393-401
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• 2009

We isolated and identified a microorganism which was excellent for ammonia oxidation in the biological control of ammonia gas in odor producing materials from organic composting. The isolated strain was tested for growth characteristics and ammonia elimination efficiency under various conditions of temperature, pH, carbon concentration and ammonia concentration. The strain was isolated from a culture broth used in a $NO_2$ producing test with Griess-Ilosvay reagent. The results of 16S rRNA sequence from the isolated strain by using BLANST (Basic Local Alignment Search Tool) and confirming RDP (Ribosomal Database Project II) and ERRD (The European Ribosomal RNA Database) indicate that the strain is related to Delftia sp. UV-Spectrophotometer (Shimadzu, UVmini-1240) was used as a microbial growth test by measuring turbidity on OD660nm and ammonia concentration was measured by Spectrophotometer (HACH, DR-4000). The optimum growth culture conditions of the ammonia oxidizer Delftia sp. were $30^{\circ}C$, pH 7, glucose concentration 1.00% and $(NH_4)_2SO_4$ 0.5 g/l. Ammonia elimination efficiency was over 94% under the same conditions.

• 한국패류학회지
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• 제19권1호
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• pp.33-40
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• 2003

To obtain the basic information on culture conditions for the larvae of Saxidomus purpuratus, experiments were conducted on the population from southern coast for (1) the success in fertilization and development from artificial fertilization among different months of a year, (2) the viability of sperms after exposure to seawater, (3) and the effects of temperature, salinity, and food organism on the survival and growth of larvae. Gametes obtained from dissection showed high rate of fertilization at all months. But the rate of development was higher only May-July. Developmental success seemed to be related with the quality of eggs at the time of fertilization. Developmental times for 2-cell, 4-cell, 8-cell, blastula, trochophore larva, and veliger larva at 20$^{\circ}C$ were 1.5, 2, 4, 18, 24, and 32 hr, respectively. Sperms could survive for more than 8 hr, however, actively swimming sperms could be found within 1 hr after exposure to seawater. It is recommended that sperms should be used for fertilization as soon as possible when they are exposed to seawater. At temperature of 35$^{\circ}C$, all the larvae died during 48 hr. Larval survival decreased when salinity was either lower than 20 psu or higher than 40 psu, and was 0% when salinity was 10 psu. Optimal range of temperature and salinity for rearing larvae of S. purpuratus were 20-25$^{\circ}C$ and 20-40 psu, respectively. Larvae grew from 111.5 to 235.3 ${\mu}$m during 21 days. Larvae fed mixed diets grew faster than unialgal diets. The fastest growth was observed when larvae were fed on the mixture of Isochrysis galbana and Nannochloris oculata.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.221-221
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• 2017

This experiment was performed to analyze variation of growth traits of soybean to determine the information of less sensitive traits under different environmental conditions. Sowing was carried out on June 2 each year and the experimental plot was laid out in three replicates by randomized complete block design with thirty soybean varieties which consisted of 19 cultivars for beancurd and soypaste, 8 cultivars for soy-sprout including 1 landrace, and 3 cultivars for cooking with rice including 1 landrace during the period 2014-2015. The weather conditions during the experimental period were quite different with extremely low precipitation and longer sunshine duration in 2015 than 2014. The variation of characteristics related to growth period such as days of growth, days of maturity, days of flowering and the 100-seed weight was less in spite of different environmental conditions. While the variation of the number of pods per plant was high. Considering growth and seed characteristics like the number of pods per plant, the number of seeds per pod and 100-seed weight which are linked directly to the yield, cultivars such as Shingi, Daewonkong, Danbaegkong, and Daepung for beancurd and soypaste, Pungwon, Haepum and Shingang for soy-sprout and Seoritae for cooking with rice were more stable and could be expected to have high yield in Suwon, the south-central part of South Korea. These results could be useful for the selection of breeding resources to develop cultivars with high stability under changeable weather condition.

• 원예과학기술지
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• 제31권4호
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• pp.400-406
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• 2013

This study was conducted to investigate the growth and flowering of African marigold (Tagetes erecta L.) and salvia (Salvia splendens F. Sello ex Ruem & Schult.) seedlings before and after storage under fluorescent lamps and green LED radiation conditions with different light intensities during storage. The both seedlings were kept under a storage room controlled at $8^{\circ}C$ air temperature and $40{\pm}10%$ relative humidity conditions. Light intensities were maintained at 15 and $30{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ during storage. In lighting treatments, dry weight of African marigold at 28 days after storage was not significantly different, and decreased approximately 29% compared to pre-storage under dark treatment. There was no significant difference in the leaf area of salvia seedlings stored under dark condition compared to before storage, but the leaf area under green light radiation with higher light intensity (treatment GH) was two times greater than before storage. The survival rate after transplanting of African marigold stored under dark condition was 10%, and days to flowering increased compared to those stored under fluorescent and green light with higher light intensity (treatment FLH, GH). Comparing to before storage, growth and flowering of the both seedlings after storage were significantly promoted by the light exposure during storage. The present experimental results show that the light intensity should be decided to maintain minimum growth during lighting storage and storage quality of the seedlings such as flowering promotion and extended blooming period after lighting treatment during storage period from the above results.

• ALGAE
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• 제31권3호
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• pp.243-256
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• 2016

Concerns about how ocean acidification will impact marine organisms have steadily increased in recent years, but there is a lack of knowledge on the responses of macroalgae. Here, we adopt an outdoor continuous-flowing mesocosm system designed for ocean acidification experiment that allows high CO₂ conditions to vary with natural fluctuations in the environment. Following the establishment of the mesocosm, five species of macroalgae that are common along the coast of Korea (namely Ulva pertusa, Codium fragile, Sargassum thunbergii, S. horneri, and Prionitis cornea) were exposed to three different CO₂ concentrations: ambient (×1) and elevated CO₂ (2× and 4× ambient), over two-week period, and their ecophysiological traits were measured. Results indicated that both photosynthesis and growth exhibited species-specific responses to the different CO₂ concentrations. Most notably, photosynthesis and growth increased in S. thunbergii when exposed to elevated CO₂ conditions but decreased in P. cornea. The preference for different inorganic carbon species (CO₂ and HCO₃⁻), which were estimated by gross photosynthesis in the presence and absence of the external carbonic anhydrase (eCA) inhibitor acetazolamide, were also found to vary among species and CO₂ treatments. Specifically, the two Sargassum species exhibited decreased eCA inhibition of photosynthesis with increased growth when exposed to high CO₂ conditions. In contrast, growth of U. pertusa and C. fragile were not notably affected by increased CO₂. Together, these results suggest that the five species of macroalgae may respond differently to changes in ocean acidity, with species-specific responses based on their differentiated photosynthetic acclimation. Understanding these physiological changes might allow us to better predict future changes in macroalgal communities in a more acidic ocean.

• 한국자원식물학회지
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• 제26권5호
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• pp.636-644
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• 2013

원료곡인 동부의 침종, 포화, 노화 조건이 동부나물 생산량과 생장반응에 미치는 영향을 검토한 결과, 흡수량은 침종 초기 2시간까지는 급속히 증가하다 이후에는 완만히 증가하였으나, 발아력과 나물 생산수율 및 잔뿌리 발생량은 침종기간(1~6시간)이 길어질수록 낮았다. 5일간 포화(수분 $96{\pm}1%$, $20^{\circ}C$)처리가 무처리나 1, 3일간 포화처리에 비해 발아력과 나물 생산수율 및 잔뿌리 발생량이 높았다. 고온 노화처리에 따른 발아력과 나물 생산수율은 무처리에 비해 낮았는데, 이상의 결과로 동부나물 재배를 위한 원료곡 전처리 방법은 5일간 포화(수분 $96{\pm}1%$, $20^{\circ}C$)처리하고 세척하여 재배하는 것이 좋은 것으로 판단된다.

• 한국약용작물학회지
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• 제23권3호
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• pp.214-222
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• 2015

This study was conducted to evaluate the growth conditions and vegetation of Peucedanum japonicum habitat in uninhabited islands of Incheon Korea. The emergence area of Peucedanum japonicum was 4.9 m distance in coastline, and altitude and slope was 7.4m and 47.5%. Soil pH, organic matter and $P_2O_5$ of habitat were 7.1, 8.3% and 29.0 mg/kg, and contents of K, Ca, Mg and Na were 0.66, 4.9, 3.4 and $1.13cmol^+/kg$, respectively. Growth plants with Peucedanum japonicum were total 55 taxa, and Gakeuldo in uninhabited islands was the most as 25 taxa. In growth plants with Peucedanum japonicum, plants of over 25% frequence per plot were Miscanthus sinensis, Dendranthema boreale, Lilium lancifolium, Hemerocallis middendorffii, Elymus dahuricus, Cirsium japonicum var. maackii, Asparagus schoberioides, Gypsophila oldhamiana, Cocculus trilobus, Silene aprica var. oldhamiana, Artemisia capillaris, Crepidiastrum denticulatum and Sedum oryzifolium. The vegetation of Peucedanum japonicum habitat was classified into Aster spathulifolius community, Miscanthus sinensis community, Dendranthema boreale community, Setaria viridis var. pachystachys community, Cirsium japonicum var. maackii community and Hemerocallis middendorffii community. According to general condition of habitat, suitable growing areas was determined to slightly acidic from mild alkaline soils.

• 한국균학회지
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• 제48권3호
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• pp.273-284
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• 2020

본 연구에서는C. indica 자실체의 안정적인 대량생산에 기반이 되는 필수적인 균사체 생산을 위한 최적 배양조건을 구명 하고자하였다. C. indica 균사체의 효율적 배양을 위한 조건은 최적 배양 온도 30-32℃, pH 6.0, 탄소원 maltose 2%, 유기질소원 yeast extract 1%, 무기질소원 NaNO₃ 0.1%, 아미노산 asparagine 0.7%, 유기산 acetic acid 0.1%, 무기염류 MnSO₄ 0.7 mM가 최적 배양조건 이었다. 새로이 MYNA로 명명한 C. indica의 최적 배지는 기존의 PDA 배지보다 균사의 생장이 균일하고, PDA 배지의 배양기간과 비교하였을 때 10.5% 단축하는 효과가 있었다. MYNA 배지는 추후 C. indica의 재배에 널리 활용될 수 있을 것으로 판단된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 춘계 학술발표대회
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• pp.138-139
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• 2003

To investigated the effect of liquid culture conditions and nutrient sources on the formation with bioactive substance of Paeilomyces japonica and Cordyceps militaris cultivated in the country, the result are as follow; The growth temperature of two mycelia is 25$^{\circ}C$ and the proper temperature for cordycepin growth is around 20$^{\circ}C$. The formation amount of bioactive substance by nutrient sources reached its peak with using 2% glucose and 1% galactose in case of carbon sources and 0.4% inorganic compound in case of nitrogen sources. Also, the ratio of C/N was optimal with 3% glucose 1% peptone. For a natural medium, most grains were sufficient but the soybean oil was superb. The formation amount of protein-binding polysaccharide that are used for anticancer substance was in proportion to the growth rate of mycelium, had lots of aeration and showed a trend of increasing when the acidity lower. and the content of structural protein showed a trend of increasing when the acidity lower. However, the content of the structural hexosamin did not get a great the effect of culture conditions and nutrient sources. The constitution of monosaccharide that organizes a protein-binding polysaccharide greatly changed in proportion to carbon sources. When Paecilomyces japonica cultured in a silkworm larvae for 30 days, the content of cordycepin was 204.5 mg/100mL as a dry weight in the fruiting body, 41.8 mg/100mL in mycelium and larva, and the content for each bottle was average 29.5 mg/100mL. In case of Cordyceps militaris for 45days, the fruiting body was 563.5 mg/100mL, the larva and the mycelium was 86.1 mg/100mL, and the content for each bottle was average 65.0 mg/100mL.

• 한국미생물·생명공학회지
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• 제10권4호
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• pp.297-305
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• 1982

한국산 미곡 밀양 25호의 저장중에 출현한 균류의 생육환경조건을 조사하고, 이들 균류에 의한 미곡의 부패를 억제하기 위하여 선정된 훈연제의 최적처리조건을 검토하여 다음과 같은 결과를 얻었다. 1. 대부분의 균류는 수분함량 19%, 상대온도 80% 이상의 미곡에서는 10~30일 이내에 잘 생육하였다. 그러나 수분함량 12.5~l3.0 %의 미곡에서는 Aspergillus candidus, Asperillus versicolor, Penicillium chrysogenum 및 Trichothecium roseum만 2개월 이내에 육안으로 생육이 확인되었고, 기타의 균류는 현미경하에서 균계의 생육만 관찰 할 수 있었다. 2. 10종류의 공시훈엽제중에서 저장균류의 생육을 억제하는데 가장 효과가 있었던 것은 ethylene oxide였으며 그 최저처리농도 및 시간은 16mg/$\ell$와 48시간이었다. 동일처리시간에서 methylbromid는 32mg/$\ell$, acrilonitrile과 methyl iodide는 64mg/$\ell$가 최저처리농도였으며, ammonia, methyl formate, ethyl formate, carbon dioxide 및 propionic acid는 주어진 처리조건에서 거의 효과가 없었다.