• Journal of Preventive Medicine and Public Health
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• v.32 no.4
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• pp.526-537
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• 1999

Objectives : It is known that the prevalence of diabetes mellitus(DM) appears to be rapidly increasing in recent times in Korea, presumably due to a westernized diet and change of life style followed by rapid economic growth. Based on the Seoul male cohort which was constructed in 1993, this study was conducted to estimate the annual incidence rates of DM through 4 years' follow up and to determine which factors are associated with DM risk in Korean middle-aged men. Methods : Among 14,533 men recruited at baseline, 559 were excluded because they reported a history of diabetes or were found to be diabetes at 1992 routine health examination. During 4 years' follow-up, 237 incident DM cases were ascertained through chart reviews and telephone contacts for those who have ever visited hospitals or clinics under suspicion of DM during 1993-1996 and the biennial routine health examinations in 1994 and 1996. Results : In this study the annual incidence of DM among the study population was estimated to be 0.5 per 100. This study showed that fasting glucose level at initial baseline examination was a powerful predictor of risk for diabetes several years later(fasting blood glucose of $\geq$ 110 mg/dl compared with $\leq$ 80 mg/dl, Hazard Ratio[HR]:15.6, 95% Confidence interval[CI]=9.1-26.6) after considering potential covariates such as age, family history, smoking and alcohol history, body mass index, physical activity, total energy intake, and total fiber intake. Adjusted hazard ratios of family history of diabetes was 1.95(95% CI=1.38-2.75); of obesity as measured by BMI(BMI $\geq$ 25.3 compared with $\leq$ 21.3) was 7.19(95% CI=3,75-13.8); of weight change during middle life(>10kg compared with $\leq$ 5) was 1.77(95% CI=1.16-2.69); of smoking(current vs none) was 1.93(95% CI=1.06-3.51); and fat intake(upper fertile compared with lower fertile) was 1.88(95% CI=1.01-3.49), while fiber intake was associated with the reduced risk(HR=0.36, 95% CI=0.19-0.67). Conclusion : The factors identified in this study indicate that the greatest reduction in risk of diabetes might be achieved through population-based efforts that promote fiber intake and reduce obesity, smoking, and fat intake.

• Korean Journal of Soil Science and Fertilizer
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• v.26 no.4
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• pp.304-307
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• 1993

An experiment was conducted to evaluate the effects of silicate and potassium application on the yield of rice irrigated with polluted water and ground water. 1. The most important component affecting plant growth in polluted water was $NH_4-N$. 2. Rice grain yield was increased by silicate and potassium application, and yield increase of silicate and potassium was higher than that of single application of silicate and potassium. 3. The ratios of $SiO_2$/T-N and $K_2O$/T-N in shoot of rirc plant increased with silicate and potassium application, but the absorption of nitrogen by rice plant was decreased. 4. Lodging index was increased by the application of polluted water, and the application of silicate and potassium reduced the lodging.

• Journal of Veterinary Clinics
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• v.23 no.3
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• pp.251-257
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• 2006

This study was performed to investigate the growth rate, hematological and serological changes of the rats when they were fed with the high(at diets supplemented with or without chitosan for five weeks. Twenty-four Sprague-Dawley male rats($235.7{\pm}10.7g$ of body weight) were randomly divided into three groups control group(C) and two treatment groups. Rats in the control group were fed with the high-fat diet containing 10% lard, 1% cholesterol and 0.5% sodium cholate(w/w) which was modified from the formula of the American Institute of Nutrition-76(AIN-76) diet. Rats in treatment groups were red with above diet supplemented with 2.5% of chitosan(CS-2.5) or 5.0% chitosan(CS-5) on the weight to weight basis, respectively. The supplementation of chitosan did not induce any significant difference on the final body weight, gain of body weight and amount of feed intake of rats in between control and treatment groups but the feed efficiency of rats in CS-5 was lower than that of rats in C(p<0.05). The hemoglobin concentrations and hematocrit values showed no significant differences among groups. In addition the values of glucose concentration, total protein, albumin, globulin and albumin/globulin(A/G) ratio showed no significant differences among groups. The values of total cholesterol and low density lipoprotein-cholesterol(LDL-C) in sera of rats in CS-5 were lower than those in both C and CS-2.5(p<0.01). The values of high density lipoprotein-cholesterol (HDL-C) in sera of rats in CS-5 were higher than in both in C and CS-2.5(p<0.05). The values of atherogenic index(AI) of rats in CS-5 were the lowest among groups(p<0.01). AI of CS-2.5 were lower than that or C(p<0.05). The values of triglyceride in sera of rats showed no significant differences among groups. The values of AST in sera of rats in CS-2.5 were lower than those in both C and CS-5(p<0.05). However ALT values showed no significant differences among groups. Therefore the supplementation of chitosan to high fat diet reduced effectively the serum lipid levels such as total cholesterol, LDL-C and triglycerides which were regarded as to cause the cardiovascular diseases moreover it elevated effectively HDL-C value which was regarded protect cardiovascular diseases.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.359-371
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• 2019

To optimize the expression and secretion of ferritin protein associated with ion storage in the mushroom, Pleurotus eryngii, a recombinant secretion vector, harboring the ferritin gene, was constructed using a pPEVPR1b vector under the control of the CaMV 35S promoter and signal sequence of pathogen related protein (PR1b). The ferritin gene was isolated from the T-Fer vector following digestion with EcoRI and HindIII. The gene was then introduced into the pPEVPR1b secretion vector, and it was then named pPEVPR1b-Fer. The recombinant vector was transferred into P. eryngii via Agrobacterium tumefaciens-mediated transformation. The transformants were selected on MCM medium supplemented with kanamycin and its expression was confirmed by SDS-PAGE and western blotting. Expression of ferritin protein was optimized by modifying the culture conditions such as incubation time and temperature in batch and 20 L airlift type fermenter. The optimal conditions for ferritin production were achieved at 25℃ and after incubating for 8 days on MCM medium. The amount of ferritin protein was 2.4 mg/g mycelia, as measured by a quantitative protein assay. However, the signal sequence of PR1b (32 amino acids) seems to be correctly processed by peptidase and ferritin protein may be targeted in the apoplast region of mycelia, and it might not be secreted in the culture medium. The iron binding activity was confirmed by Perls' staining in a 7.5% non-denaturing gel, indicating that the multimeric ferritin (composed of 24 subunits) was formed in P. eryngii mycelia. Mycelium powder containing ferritin was tested as a feed additive in broilers. The addition of ferritin powder stimulated the growth of young broilers and improved their feed efficiency and production index.

• Korean Journal of Ecology and Environment
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• v.39 no.3 s.117
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• pp.296-309
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• 2006

This study aimed to analyze water quality (temperature, secchi depth, DO, $NH_4$, $NO_3$, $PO_4$, TN, TP, chlorophyll a) and trophic state index during the period of year 2000 ${\sim}$ 2004 in Lake Juam, Lake Dongbok and Lake Youngsan. Lakes Juam and Dongbok except Lake Youngsan were stratified during warm seasons. Water turbidity estimated by secchi disk depth was the highest in Lake Youngsan compared with other lakes. DO concentrations were low in the bottom water when chlorophyll a was high in Lake Juam and Dongbok. Nutrient concentrations were higher in Lake Youngsan than other lakes whereas chlorophyll a was highest in Lake Dongbok. Lake Youngsan was the most eutrophic compared to other two lakes based on the Trophic State Idex (TP) and TSI (SD), The TSI (CHL) was high but the TSI (TP) were low in Lake Juam and Dongbok. These results suggest phytoplankton may be limited by phosphates (P) in Lake Juam and Dongbok whereas light availability in the water column may affect growth of phytoplankton in Lake Youngsan.

• Journal of Oriental Neuropsychiatry
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• v.16 no.2
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• pp.25-34
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• 2005

Background and Objectives There was no clinical data except literary study on mutual correlation with autistic disorder in Western medical diagnosis and five kinds of flaccidity in infants(五軟), five kinds of retardation(五遲) in Oriental medical diagnosis. This study was performed to investigate the correlation of five kinds of flaccidity in infants(五軟), five kinds of retardation(五遲) to the children with autistic disorder and to set the time table for clinical diagnosis of developmental retardation by making a comparative study of normal developmental children so we can treat the children with autistic disorder in good time. Method We made the comparative study of interview sheets recorded by parents of total 163 children who were diagnosed as autistic disorder who visited HaeMa Oriental Medical Clinic with interview sheets recorded by parents of generally accepted normal developmental children(263) and then we took statistics. Results : 1. There was significant correlation with speech and walking among five kinds of flaccidity in infants(五軟), five kinds of retardation(五遲) in autistic disorder statistically and clinically in comparison with normal children. 2. There wasn't significant correlation with growth time of tooth among five kinds of flaccidity in infants(五軟), five kinds of retardation(五遲) on diagnosis of autistic disorder in comparison with normal children. 3. There was significant correlation with retardation of times going to toilet by oneself (it does not consist in five kinds of flaccidity in infants(五軟), five kinds of retardation(五遲).) in both of autistic disorder. Conclusion Autistic disorder was significantly correlated with the faculty of speech(語遲), retardation in walking out(行遲) of five kinds of flaccidity in infants(五軟), five kinds of retardation(五遲). We need to concrete the index of diagnosis, because it is so difficult to measure times and register retardation in tooth eruption(齒遲), retardation in hair-growing(髮遲), debility of neck and nape(頭項軟), flaccidity of extremities(手軟) and flaccidity of muscle(肌肉軟). And we can also use times going to toilet by oneself as one of diagnostic criteria because of its significant correlation. It is required to make early diagnosis of five kinds of flaccidity in infants(五軟), five kinds of retardation(五遲) using these criteria, and to treat them early by oriental medicine.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.2
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• pp.254-260
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• 1997

Effects of kimchi extracts on the immune response related to its antitumor activity in vitro and in vivo were investigated. The extracts of kimchi fermented for 0(fresh) and 3 weeks at $5^{\circ}C$ showed a direct cytotoxic effect on sarcoma-180 cells, tumor cells in vitro. Methanol extract(4mg/ml), MSF(methanol soluble fraction : 3mg/ml) and hexane extract(fresh : 2.0mg/ml, 3 weeks : 0.3mg/ml) of the kimchi(3 weeks, $5^{\circ}C$) markedly decreased the total numbers of sarcoma-180 cells, but not their viability. The phagocytic activity of peritoneal macrophage of mice was significantly augmented by these extracts of the kimchi compared with that of control in vitro and in vivo. These extracts also raised the phagocytic index, indicating that the number of phagocytized microbes per macrophage increased. Thus, kimchi might show a anti-tumor activity by enhancing the phagocytic cell activities.

• Clinical and Experimental Pediatrics
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• v.51 no.10
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• pp.1052-1057
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• 2008

Purpose : This study was to examine the relations of perception of physique with self-esteem, sociality and problematic behaviors in children. Methods: Four hundred sixty five (231 boys and 234 girls) children were divided into three groups according to their height or body mass index according to the physical growth standard table had been presented in 2007 by the Korean Pediatric Society. The group 1, 2, and 3 was short, borderline and normal or obese, overweight and normal, respectively. Their perception and satisfaction of height and weight were investigated, and their self esteem and behavior styles were examined with Self-Esteem Inventory (SEI) and Korean Child Behavior Checklist (K-CBCL). We compared the numbers of appropriate perception, satisfaction, self-esteem and problematic behaviors in each group, and analyzed the relationship among perception of physique, self-esteem, sociality, and behavioral characteristics. The collected data were analyzed with chi-square test, ANOVA and Pearson correlation coefficient used with SPSS (ver. 12.0). Results : There were significant mismatches among their real physique perception and satisfaction (P<0.05). There were no significant differences of their self-esteem, sociality, and problematic behaviors according to their actual height and weight. There were significant differences of their self-esteem according to their perception or satisfaction of their height and weight (P<0.01). Conclusion : The children who considered themselves have short stature or obesity had problematic behaviors or low self-esteem. Therefore, children should be educated to have the appropriate perception of their own body image.

• Korean Journal of Soil Science and Fertilizer
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• v.5 no.1
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• pp.33-39
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• 1972

For the increase of productivity, canopy structure and nutritional status in various productivity grades (high>700, medium>600 and low>500kg/l0a as head rice) were investigated in a study using Jinheung(Oryza sativa L.) for the increase of producticity with following results: 1. High yield plot showed greater leaf area and slow decrease of it after heading stage. While low yield plot had rapidly decrease of leaf area. 2. High yield plot showed higher light transmission ratio (LTR) durnig early grow stage, low LTR. during late grow stage, and higher leaf area per LTR. 3. Net assimilation ratio was decrease with the increase of leaf area index (LAI) and it was always higher in high yield plot. 4. High yield plant had greater amount of total nitrogen uptake, slow uptake rate, and considerable uptake after heading while low yield plant showed higher uptake of nitrogen in early stage of growth and no uptake after heading. 5. The percent ratio starch at heading to total at harvesting was 40% for low yield plant and 10% for highyield plant inderting that high yield depends much on past-heading starch synthesis. 6. Main factor determining secondary yield was ripened grain ratio.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.1100-1107
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• 2012

Bacterial endophytes may be important factors in plant growth and ecologically relevant functions in rice. Using pyrosequencing technology, we analyzed the composition of endophytic bacterial communities that colonized the roots of rice cultivated in long-term fertilized (APK) and non-fertilized (NF) paddy soils. A total of 1,900 reads were obtained from 2 samples. All sequences were classified into 177 OTUs (APK sample) or 72 OTUs (NF sample) at a 97% similarity cut-off. Twenty-two OTUs were shared between the 2 samples, and these were also the most dominant OTUs in both samples. Proteobacteria was the most dominant phylum with 90.2%, followed by Actinobacteria (7.1%) and Bacteroidetes (1.1%). Furthermore, Pseudomonas was the most abundant genus in both samples. We observed clear differences in the structure of the endophytic bacterial community structure between the 2 samples. Notably, the distributions of Alphaproteobacteria and Gammaproteobacteria were markedly different. The diversity index of the APK sample was higher than that of the NF sample. These findings showed that the endophytic bacterial community of rice roots was affected by the presence of fertilizers in the rice field soil.