• IMMUNE NETWORK
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• 제9권6호
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• pp.236-242
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• 2009

Background: Melanoma is the most fatal form of skin cancer due to its rapid metastasis. Recently, several studies reported that selenium can induce apoptosis in melanoma cells. However, the precise mechanism remains to be elucidated. In this study, we investigated the effect of selenium on cell proliferation in murine melanoma and on tumor growth and metastasis in C57BL/6 mice. Methods: Cell proliferation was measured by MTT assay in selenium-treated melanoma cells. Cell cycle distribution was analysized by staining DNA with propidum iodide (PI). mRNA and protein expression related to cell cycle arrest was measured by reverse transcription PCR and western blot. Tumor growth and metastasis was measured by in vivo model. Results: Selenium was suppressed the proliferation of melanoma cells in a dose dependent manner. The growth inhibition of melanoma by selenium was associated with an arrest of cell cycle distribution at G0/G1 stage. The mRNA and protein level of CDK2/CDK4 was suppressed by treatment with selenium in a time-dependent manner. In vivo, tumor growth was not suppressed by selenium; however tumor metastasis was suppressed by selenium in mouse model. Conclusion: These results suggest that selenium might be a potent agent to inhibit proliferative activity of melanoma cells.

• 한국환경농학회지
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• 제33권4호
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• pp.350-357
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• 2014

$CaCl_2$나 NaCl과 같은 제설제들은 겨울철에 교통사고예방과 상해방지를 위해서 빈번히 사용된다. 제설제가 사고를 줄이는데 기여를 해오고 있지만, 농업 및 임업 생산성 감소, 토양 및 수질 오염 등의 부작용도 보고되고 있다. 본 연구는 겨울철 재배가 활발한 밀, 보리, 시금치의 발아 생육에 대한 제설제의 영향을 조사하였다. 제설제의 농도가 3%이상인 경우, 노출된 밀, 보리, 시금치의 발아는 대조구에 비해 유의성이 있는 감수성을 보였고, 시금치가 가장 감수성이 높았다. 유묘에 대한 제설제 분무는 잎의 성장에 영향을 주지 않았다. 작물이 지속적으로 제설제에 처리된 토양에 노출되었을 때 작물의 발아는 제설제 2% 이상농도에서도 영향을 받았다. 제설제에 노출된 유묘성장은 $CaCl_2$, NaCl 처리구 모두 밀과 보리에 비슷한 저해효과를 보였고, 시금치가 가장 높은 감수성을 보였다. 1% 제설제가 처리된 토양에서 1달동안 성장한 유묘의 줄기와 뿌리는 대조구에 비해서 성장이 저해되었다. 이상의 결과로부터 제설제에 대한 작물의 감수성은 NaCl> $CaCl_2$ > mixture ($CaCl_2$ + NaCl) 임을 알 수 있었다. 모든 조사작물 유묘의 생체량은 1.5-4배 감소하였다. 이상의 결과는 염화물계 제설제가 토양에 들어감으로써 작물의 발아, 성장, 생체량의 감소에 영향을 준다는 것을 보여준다.

• 한국환경농학회지
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• 제33권4호
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• pp.372-380
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• 2014

본 연구는 시비처리가 상록 참나무류(가시나무, 붉가시나무 및 종가시나무) 유묘의 용기 내 생장에 미치는 영향을 구명하고자 실시되었으며, 시비수준은 무시비구와 함께 수용성 복합비료(N:P:K=19:19:19, v/v)를 1000, 2000, $3000mg{\cdot}L^{-1}$ 수준으로 조절하여 시비하였다. 시비처리는 이들 3 수종의 간장과 근원경 생장 그리고 건물생산량을 증가시켰으며, 시비 수준이 증가할수록 이들 생장도 증가하였다. H/D율과 T/R율도 3 수종 모두 시비수준이 증가할수록 커지는 경향을 보였다. 광합성률은 3 수종 모두 시비처리에 의해 또 시비 수준이 높아질수록 높아진 것으로 나타났다. 뿌리 형태 특성 분석에서, 전체 뿌리길이의 경우 시비농도가 증가할수록 긴 것으로 조사되었다. 투영단면적, 표면적 및 뿌리부피의 경우도 전체 뿌리길이의 특성과 유사한 경향으로 시비처리에 따른 증가가 관찰되었다. 이상과 같은 결과로 볼 때 뿌리발달이 뛰어나며 묘목품질지수가 높은 상록 참나무류의 1년생 용기묘생산에 적합한 시비수준은 $2000mg{\cdot}L^{-1}$ 정도인 것으로 판단된다.

• 생물정신의학
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• 제12권2호
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• pp.143-150
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• 2005

목 적: 많은 연구에서 정신분열병에서 염증반응체계의 활성화와 사이토카인의 변화가 병태생리학적 및 원인적 역할을 하는 것으로 보고되어 왔으며, 여기에는 type 1 Thelper cell(Th1), type 2 T helper cell(Th2), type 3 T helper cell(Th3)의 조절 이상이 제시되고 있다. 본 연구에서는 정신분열병 환자에서 항정신병 약물 치료 전후로 Th1 사이토카인인 interleukin-12(IL-12), Th3 사이토카인인 transforming growth factor-${\beta}1$(TGF-${\beta}1$)의 혈장 농도를 측정하였다. 방 법: 23명의 정신분열병 환자군과 31명의 정상대조군에서 IL-12와 TGF-${\beta}1$ 농도를 측정하였고 정신분열병 환자군에서는 8주간 항정신병 약물로 치료 후 다시 IL-12와 TGF-${\beta}1$의 농도를 측정하였다. 또한 정신분열병 환자군에서 치료전과 8주간 치료 후, 2차례에 걸쳐 Brief psychiatric rating scale(BPRS)를 측정하였다. 결 과: 치료전 IL-12 농도와 TGF-${\beta}1$ 농도 모두 정상대조군보다 환자군에서 유의하게 높게 나타났다. 8주간의 치료 후 TGF-${\beta}1$ 농도는 유의하게 감소하여 정상대조군의 농도와 차이를 보이지 않게 된 반면, IL-12의 농도는 유의하지 않은 감소를 보였다. BPRS 점수의 변화 및 IL-12 및 TGF-${\beta}1$의 농도의 변화 사이에는 유의한 상관관계가 없었다. 결 론: 정신분열병의 병태생리학에 사이토카인의 이상이 관여할 수 있으며, TGF-${\beta}1$이 중요한 역할을 하는 것으로 생각된다.

• Journal of Ecology and Environment
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• 제44권1호
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• pp.8-25
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• 2020

Background: Species of the genera Eucalyptus, Cupressus, and Pinus are the most widely planted tree species in the country in general and in Chilimo dry Afromontane forest in particular. Eucalyptus covers 90% of the total planted forest area in the country. However, only limited information exists in the country regarding aboveground biomass (AGB), belowground biomass (BGB), growth, and yield. This study was conducted to assess the variables on 25 and 30 years of age for three planted species: Cupressus lusitanica, Eucalyptus saligna, and Pinus patula in Chilimo plantation forest, in the Central Highlands of Ethiopia. A two-times inventory was conducted in 2012 and 2017. A total of nine square sampled plots of 400 ㎡ each, three plots under Cupressus lusitanica, 3 Eucalyptus saligna, and 3 Pinus patula were used for data collection. Data on height, diameter, soil, and tree stumps were collected. Percent C, % N, and bulk density was performed following chemical procedure. Results: The aboveground biomass ranged from 125.76 to 228.67 t C ha^-1 and the basal area and number of stems from 3.76 to 25.50 ㎡ ha^-1 and 483 to 1175 N ha^-1, respectively. The mean annual basal area and volume increment were between 0.97 and 1.20 ㎡ ha^-1 year^-1 and 10.79 and 16.22 ㎥ ha^-1 year^-1. Both carbon and nitrogen stock of the planted forest was non-significant among the tree species. Conclusion: The aboveground biomass, growth, and yield significantly varied among the species. Cupressus lusitanica had the highest aboveground biomass, volume, and basal area, while Eucalyptus saligna had the lowest value. To a depth of 1 m, total carbon stored ranged from 130.13 to 234.26 t C ha^-1. The total annual carbon sequestration potential was 12,575.18 t CO₂ eq. Eucalyptus has the highest carbon stock density and growth rate than other species.

• Asian Pacific Journal of Cancer Prevention
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• 제15권1호
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• pp.489-494
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• 2014

Background: Psychiatric patients appear to be at lower risk of cancer. Some antipsychotic drugs might have inhibitory effects on tumor growth, including penfluridol, a strong agent. To test this, we conducted a study to determine whether penfluridol exerts cytotoxic effects on tumor cells and, if so, to explore its anti-tumor mechanisms. Methods: Growth inhibition of mouse cancer cell lines by penfluridol was determined using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Cytotoxic activity was determined by clonogenic cell survival and trypan blue assays. Animal tumor models of these cancer cells were established and to evaluate penfluridol for its anti-tumor efficacy in vivo. Unesterified cholesterol in cancer cells was examined by filipin staining. Serum total cholesterol and tumor total cholesterol were detected using the cholesterol oxidase/p-aminophenazone (CHOD-PAP) method. Results: Penfluridol inhibited the proliferation of B16 melanoma (B16/F10), LL/2 lung carcinoma (LL/2), CT26 colon carcinoma (CT26) and 4T1 breast cancer (4T1) cells in vitro. In vivo penfluridol was particularly effective at inhibiting LL/2 lung tumor growth, and obviously prolonged the survival time of mice bearing LL/2 lung tumors implanted subcutaneously. Accumulated unesterified cholesterol was found in all of the cancer cells treated with penfluridol, and this effect was most evident in LL/2, 4T1 and CT26 cells. No significant difference in serum cholesterol levels was found between the normal saline-treated mice and the penfluridol-treated mice. However, a dose-dependent decrease of total cholesterol in tumor tissues was observed in penfluridol-treated mice, which was most evident in B16/F10-, LL/2-, and 4T1-tumor-bearing mice. Conclusion: Our results suggested that penfluridol is not only cytotoxic to cancer cells in vitro but can also inhibit tumor growth in vivo. Dysregulation of cholesterol homeostasis by penfluridol may be involved in its anti-tumor mechanisms.

• 한국약용작물학회지
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• 제28권1호
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• pp.1-8
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• 2020

Background: This study was conducted to investigate the effects of NaCl concentration on the photosynthetic parameters, chlorophyll fluorescence and growth characteristics of Crepidiastrum sonchifolium. Methods and Results: As treatments, we subjected C. sonchifolium plants to four different concentrations of NaCl (0, 50, 100 and 200 mM). We found that the photosynthetic parameters maximum photosynthesis rate (P_{N max}), net apparent quantum yield (Φ), maximum carboxylation rate (V_cmax), and maximum electron transport rate (J_max) were significantly reduced at an NaCl concentration greater than 100 mM. In contrast, there was an increase in water-use efficiency with increasing NaCl concentration, although in terms of growth performances, leaf dry weight, root dry weight, stem length, and total dry weight all decreased with increasing NaCl concentration. Furthermore, leakage of electrolytes, as a consequence of cell membrane damage, clearly increased in response to an increase in NaCl concentration. Analysis of the polyphasic elevation of chlorophyll a fluorescence transients (OKJIP) revealed marked decrease in flux ratios (Φ_PO, Ψ_O and Φ_EO) and the PI_abs, performance index in response to treatment with 200 mM NaCl, thereby reflectings the relatively reduced state of photosystem II. This increase in fluorescence could be due to a reduction in electron transport beyond Q^-_A. We thus found that the photosynthetic parameters, chlorophyll fluorescence and growth characteristics of C. sonchifolium significantly increased in response to treatment with 200 mM NaCl. Conclusions: Collectively, the findings of this study indicate that C. sonchifolium shows relatively low sensitivity to NaCl stress, although photosynthetic activity was markedly reduced in plants exposed to 200 mM NaCl.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.77-77
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• 2017

The sensitivities to photoperiod and temperature give guidance to choose an adaptable genotype for specific area in soybean production. However, there is insufficient information about the variation of sensitivities to photoperiod and temperature with wide genetic background. We investigated the sensitivities to photoperiod and temperature using 82 soybean mini core collection lines provided by NIAS gene bank of Japan. The seeds were sown on 28 May and 4 August in 2015, 24 May and 5 August in 2016 at field in Saga, Japan ($33^{\circ}$ 14' 32'' N, $130^{\circ}$ 17' 28'' E) for the early (average photoperiod and temperature: 15.2 h and $25.1^{\circ}C$) and late (13.6 h and $27.2^{\circ}C$) sowing respectively. The plants were also grown in the growth chamber under 12 h photoperiod with three temperature regimes (day/night temperature: $25/18^{\circ}C$, $28/22^{\circ}C$ and $33/28^{\circ}C$). Emergence date, days to first flower were recorded with 10 plants in the field and 2 plants in the growth chamber for each line. The data for daily average temperatures and photoperiodic hours were collected from weather station. The days from emergence to first flower open (DEF) were varied from 23-92 (2015 and 2016) in early sowing whereas 18-68 (2015) and 18-59 (2016) in late sowing. The shortened DEF in late sowing could be caused by both short photoperiod and high temperature in late sowing. However, the accumulated temperatures during emergence to first flower open (ATEF) were less variable in comparison with DEF, suggesting the ATEF is dependent mostly on the photoperiod. The ATEF were found same between early and late sowing in some early flowering lines (e.g. $686.7^{\circ}C$ and $687.6^{\circ}C$ in HEUKDAELIPS, $728.8^{\circ}C$ and $706.3^{\circ}C$ in WILLIAMS'82) which indicated that these would be insensitive to day length. In the growth chamber experiment, the variation in both DEF and ATEF was a little greater at low temperature ($25/18^{\circ}C$) but almost same at middle ($28/22^{\circ}C$) and high ($33/28^{\circ}C$) temperatures. Since the less differences in ATEF were found between the three temperatures, it is suggested that the temperature plays only a quantitative effect on the flower initiation, and the large ATEF in some lines may indicate the stronger photosensitivity even at 12 h or longer juvenile phase. Some lines with the lowest ATEF regardless of growth conditions, such as FISKEBY V, KE 32 (ATEF: 559.6-666.5, 587.7-709.5) might lack the sensitivities to both photoperiod and temperature. The results suggested that soybean genotypes has wider variation in sensitivity to photoperiod, whereas less variation to temperature.

• 한국환경농학회지
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• 제37권1호
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• pp.28-33
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• 2018

수박 휴작기에 풋거름 작물로 헤어리베치와 호밀을 재배한 후 토양으로의 환원이 후작물 수박의 생육과 수량 그리고 토양의 질산태질소 함량에 미치는 영향을 구명하고자 하였다. 파종 후 151일간 재배한 후에 헤어리베치와 호밀의 토양 질소 환원량은 각각 79 kg/ha와 88 kg/ha였다. 헤어리베치와 호밀은 시설하우스 한 동씩 두 동의 면적에 풋거름 작물구, 풋거름작물에 요소 25%, 50%, 75% 처리구, 요소 100% 등 5처리를 각각 두었다. 수박의 생육은 각각의 헤어리베치 및 호밀 처리에서 동일한 생육을 나타냈고 수박의 수량에서도 처리간의 통계적인 유의성은 없었다. 토양의 질산태 질소함량은 수박 정식 후 재배일수가 증가할수록 지속적으로 감소하여 정식 후 75일째에 가장 낮은 함량을 나타내었는데 호밀처리구는 호밀만 투입만 처리가 52 mg/kg으로 가장 낮았고, 헤어리베치 처리간 비교에서도 마찬가지로 헤어리베치만 공급한 처리가 가장 낮은 44 mg/kg이었다. 헤어리베치와 호밀 등 녹비작물만의 투입으로도 수박의 재배기간 동안에 토양의 질산태 질소가 40 mg/kg 이상으로 존재하여 화학비료의 추가적인 시비 없이도 동일한 생육을 보인 것으로 판단되었다. 하지만 수박의 수량과 재배기간에의 토양 중 질산태질소와의 관계에 대한 추가적인 연구가 필요할 것으로 사료된다.

• Fisheries and Aquatic Sciences
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• 제22권11호
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• pp.25.1-25.9
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• 2019

Background: Due to the continuous demand for fish coupled with decline in capture fisheries, there is the need to increase aquaculture production to meet the demand. Aquaculture is faced with high cost of feeding since fish oil and fish meal are expensive. In view of this, there are calls to explore alternatives that are cheap and reliable. Objectives: This study on Oreochromis niloticus was conducted to evaluate the effects of replacing fish oil (FO) with palm oil (PO) at 0%, 25%, 50%, 75%, and 100% on muscle fatty acid and proximate composition as well as growthrelated enzyme activities and mRNA expression. Methods: Oreochromis niloticus were fed five experimental diets (33% crude protein and 10% crude lipid) for 8 weeks. Feed had variation in fish oil and palm oil contents. After the 8 weeks feeding trial, five fish were sampled from each tank (15 from each treatment) and euthanized using an excess dose of tricaine methane sulfonate (MS-222 at 200 mg/L). Fatty acid and enzyme activities were analyzed using standard protocols. Also, RT-qPCR was used to quantify the expression levels of selected growth-related genes. Results: Fish fed 25% PO recorded the least muscle protein content and was significantly lower than the group fed 100% PO. Paired box protein 7 (Pax-7) enzyme activity was significantly higher in the group fed 50% PO compared to the groups fed 25% PO and 100% PO, while caplain-3 (Capn-3) was significantly lower in the group fed 0% PO compared to all other groups. There was a significant difference among treatments with respect to mRNA expression of Pax-7 and Capn-3. Group fed 25% PO had significantly lower mRNA expression of Pax-7, while the group fed 75% PO recorded significantly higher mRNA expression of Capn-3 compared to groups fed 0% PO, 25% PO, and 100% PO. Pearson's correlation analysis revealed that Igf-I and Igf-II mRNA expression have significant correlation with n-3 polyunsaturated fatty acids content in muscle. Conclusion: The results suggest muscle protein content could be modified if FO is replaced with PO. Also, mRNA expression of Pax-7 and Capn-3 is affected by replacing FO with PO.