• Asian Pacific Journal of Cancer Prevention
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• 제15권9호
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• pp.4039-4044
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• 2014

Background: The aim of this study was to investigate the anti-cancer effects and mechanisms of immunochemotherapy of 5-fluorouracil (5-FU) and interleukin-2 (IL-2) on non-small cell lung cancer (NSCLC) A549 cells. Materials and Methods: In order to detect whether 5-FU+IL-2 could effectively inhibit tumor growth in vivo, we established an A549-bearing nude mouse model. The cytotoxicity of natural killer (NK) cells was evaluated using a standard chromium release assay. To evaluate the relevance of NK cells in 5-FU+IL-2-mediated tumor inhibitory effects, we depleted NK cells in A549-bearing mice by injecting anti-asialo-GM-1 antibodies. Effects of 5-FU+IL-2 on the expression and promoter activity of NKG2D ligands (MICA/MICB) in A549 cells in vitro were also assessed. Results: In A549-bearing nude mice, combination therapy significantly inhibited tumor growth in comparison with monotherapy with 5-FU or IL-2 and enhanced the recognition and lysis of tumor cells by NK cells. Further study of mechanisms showed that NK cells played a vital role in the anticancer immune response of 5-FU+IL-2 immunochemotherapy. In addition, the combination therapy synergistically stimulated the expression and promoter activity of MICA/MICB. Conclusions: 5-FU and IL-2 immunochemotherapy significantly inhibited tumor growth and activated NK cytotoxicity in vivo, and these effects were partly impaired after depleting NK cells in tumor-bearing mice. Combination treatment of 5-FU and IL-2 upregulated the expression and the promoter activity of MICA/MICB in A549 cells, which enhanced the recognition of A549 cells by NK cells. All of the data indicated that immunochemotherapy of 5-FU and IL-2 may provide a new treatment option for patients with lung cancer.

• 한국환경농학회지
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• 제34권2호
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• pp.111-119
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• 2015

BACKGROUND: Ethychlozate (ECZ) is a plant growth regulator of synthetic auxin for agricultural commodities (ACs). Accurate and sensitive method to determine ECZ in diverse ACs on global official purpose is required to legal residue regulation. As the current official method is confined to the limited type of crops with poor validation, this study was conducted to improve and extend the ECZ method using high-performance liquid chromatography (HPLC) in all the registered crops with method verification. METHODS AND RESULTS: ECZ and its acidic metabolite (ECZA) were both extracted from acidified samples with acetone and briefly purified by dichloromethane partition. ECZ was hydrolyzed to form ECZA and the combined ECZA was finally purified by ion-associated partition including hexane-washing. The instrumental quantitation was performed using HPLC/ FLD under ion-suppression of ECZA with no interference by sample co-extractives. The average recoveries of intra- and inter-day experiment ranged from 82.0 to 105.2% and 81.7 to 102.8%, respectively. The repeatability and reproducibility for intra- and inter-day measurements expressed as a relative standard deviation was less than 8.7% and 7.4%, respectively. CONCLUSION: Established analytical method for ECZ residue in ACs was applicable to the nation-wide pesticide residues monitoring program with the acceptable level of sensitivity, repeatability and reproducibility.

• 한국화재소방학회논문지
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• 제33권6호
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• pp.20-27
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• 2019

본 연구는 화재 시 열출력 급상승 구간에 대한 정확한 열방출율을 예측하기 위한 새로운 함수를 개발하여 제안하는 것을 목적으로 하였다. 현재 화재공학에서 사용되고 있는 'αt²' 곡선은 화재시스템 공학 관점에서 비효율적이며 실효성 저하를 초래하므로 열방출율의 예측오차를 최소화시킬 필요가 있다. 'αt²'과 비교하여 보다 논리적인 배경과 형태적으로 유사성을 가진 로지스틱 함수 이론을 기반으로 화재 급성장 구간은 물론 화재 초기 단계까지 적용 가능한 새로운 예측 함수를 개발하였다. 개발된 함수는 더 넓은 화재성장 구간에서 정확도 높은 예측결과를 갖는 것으로 본 연구에서 증명되었다. 이 연구결과는 향후 화재성장패턴 연구의 개발과 함께 화재공학의 발전을 위해 적용될 것이다.

• Archives of Plastic Surgery
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• 제41권6호
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• pp.647-653
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• 2014

Background Administration of growth factors has been associated with increased viability of composite grafts greater than 1-cm in diameter. Platelet-rich plasma (PRP) contains many of the growth factors studied. In this study, we evaluate the effect of PRP injection on composite graft viability and the proper time for injection. Methods A total of 24 New Zealand White rabbits were divided into four groups. Autologous PRP was injected into the recipient sites three days before grafting in group 1, on the day of grafting in group 2, and three days after grafting in group 3. Group 4 served as control without PRP administration. Auricular composite grafts of 3-cm diameter were harvested and grafted back into place after being rotated 180 degrees. Median graft viability and microvessel density were evaluated at day 21 of graft via macroscopic photographs and immunofluorescent staining, respectively. Results The median graft survival rate was 97.8% in group 1, 69.2% in group 2, 55.7% in group 3, and 40.8% in the control group. The median vessel counts were 34 (per ${\times}200$ HPF) in group 1, 24.5 in group 2, 19.5 in group 3, and 10.5 in the control group. Conclusions This study demonstrates that PRP administration is associated with increased composite graft viability. All experimental groups showed a significantly higher survival rate and microvessel density, compared with the control group. Pre-administration of PRP was followed by the highest graft survival rate and revascularization. PRP treatments are minimally invasive, fast, easily applicable, and inexpensive, and offer a potential clinical pathway to larger composite grafts.

• 한국약용작물학회지
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• 제23권6호
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• pp.460-467
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• 2015

Background : The aim of the present study was to identify an effective physicochemical control method to reduce Fusarium species infestation in adlay (Coix lacryma-jobi L.) before and after harvesting. Methods and Results : We observed that prochloraz emusifiable concentrate and hexaconazol prochloraz emusifiable concentrate strongly inhibited the mycelial growth of 10 Fusarium species. Strong growth inhibitions and cell lysis were observed following treatment with 4% NaOCl solution. The total number of fungi detected were lower follwing treatment with thiophanatemethyl triflumizole wettable powder ($1.1{\times}10^4CFU/g$), hexaconazol prochloraz emulsifiable concentrate ($1.2{\times}10^4CFU/g$), carboxin thiram dustable powder ($1.6{\times}10^4CFU/g$) and prochloraz emulsifiable concentrate ($1.7{\times}10^4CFU/g$) than in the non-treated control ($7.7{\times}10^4CFU/g$). The reduction of Fusarium fungi varies with the concentration and soaking time of NaOCl solution. Fungal detection was not observed after soaking in NaOCl solution for 24 h and harmful effects were not observed for plant growth by NaOCl after soacking for 6 - 12 h. Conclusion : Soaking seed for 6 - 12 h in 4% NaOCl could be an effective method of disinfectant treatment for the control of Fusarium fungi in adlay seeds.

• Journal of Ecology and Environment
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• 제44권1호
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• pp.45-53
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• 2020

Background: The black soldier fly (Hermetia illucens) is gaining attention as an efficient decomposer of food waste. However, recalcitrant compounds such as plastics mixed into food waste may have negative effects on its growth and survival. Moreover, its efficiency of food waste degradation may also be affected by plastics. In addition, salt (NaCl) can also be present in high concentrations, which also reduces the efficiency of H. illucens-mediated food waste treatment. In this study, we assessed the growth of black soldier fly larvae (BSFL) reared on food waste containing polyethylene (PE) and polystyrene (PS) and NaCl. The weight of BSFL was measured every 2-4 days. Survival and substrate reduction rates and pupation ratio were determined at the end of the experiment. Results: The total larval weight of Hermetia illucens reared on food waste containing PS was greater than that of the control on days 20 and 24. However, the survival rate was lower in the group treated with 5% PS, as was substrate reduction in all PS-treated groups. The weight of BSFL reared on food waste containing PE was lower than that of the control on day 6. PE in food waste did not affect the survival rate, but the pupation ratio increased and substrate consumption decreased with increasing PE concentrations. Regardless of the plastic type, the addition of NaCl resulted in decreased larval weight and pupation ratio. Conclusions: Larval growth of black soldier fly was inhibited not by plastics but by substrate salinity. Additional safety assessments of larvae reared on food waste containing impurities are needed to enable wider application of BSFL in vermicomposting.

• Nutrition Research and Practice
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• 제8권5호
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• pp.521-525
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• 2014

BACKGROUND/OBJECTIVES: Artemisinin (AT), an active compound in Arternisia annua, is well known as an anti-malaria drug. It is also known to have several effects including anti-oxidant, anti-inflammation, and anti-cancer activities. To date, the effect of AT on vascular disorders has not been studied. In this study, we investigated the effects of AT on the migration and proliferation of vascular smooth muscle cells (VSMC) stimulated by platelet-derived growth factor BB (PDGF-BB). MATERIALS/METHODS: Aortic smooth muscle cells were isolated from Sprague-Dawley rats. PDGF-BB stimulated VSMC migration was measured by the scratch wound healing assay and the Boyden chamber assay. Cell viability was determined by using an EZ-Cytox Cell Viability Assay Kit. The production of reactive oxygen species (ROS) in PDGF-BB stimulated VSMC was measured through $H_2DCF$-DA staining. We also determined the expression levels of signal proteins relevant to ROS, including measures of extracellular signal-regulated kinase (ERK) 1/2 measured by western blot analysis and matrix metalloproteinase (MMP) 9 measured by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: AT ($10{\mu}M$ and $30{\mu}M$) significantly reduced the proliferation and migration of PDGF-BB stimulated VSMC in a dose-dependent manner. The production of ROS, normally induced by PDGF-BB, is reduced by treatment with AT at both concentrations. PDGF-BB stimulated VSMC treated with AT ($10{\mu}M$ and $30{\mu}M$) have reduced phosphorylation of ERK1/2 and inhibited MMP9 expression compared to untreated PDGF-BB stimulated VSMC. CONCLUSIONS: We suggest, based on these results, that AT may exert an anti-atherosclerotic effect on PDGF-BB stimulated VSMCs by inhibiting their proliferation and migration through down-regulation of ERK1/2 and MMP9 phosphorylation.

• 벤처창업연구
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• 제3권2호
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• pp.1-29
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• 2008

본 연구의 목적은 새로운 창업교육프로그램의 모델을 도출하여 창업대학원의 사례에 적용시켜 개선방안을 도출하는데 있다. 먼저 국내학자들이 창업교육에 대한 선행연구를 검토하고 이를 바탕으로 국외학자들의 창업교육에 대한 관점을 이론적 배경으로 하였으며, 미국의 창업교육대학원과 일본의 창업교육대학원의 교육프로그램을 분석하여 새로운 창업교육프로그램의 모델을 도출하였다. 창업교육프로그램의 모델은 창업교육의 이론적 범위, 교육체계 및 실천적 모델을 도출하였으며, 이를 창업대학원의 사례에 적용하여 개선방안을 제시하였다. 본 연구의 결과는 교육기관의 교육목적 및 교육대상에 따라 세부교과목은 변경될 수 있으나, 일반적으로 창업교육프로그램을 개발하는 과정에 대한 지침을 제공해 줄 수 있으며, 특정 목적에 합당하게 체계화된 교육의 교과과정을 개발하는데 적용시킬 수 있는 통찰력을 제공해 줄 수 있다.

• 한국환경농학회지
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• 제35권2호
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• pp.97-103
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• 2016

BACKGROUND: Clay mineral is well known to improve physico-chemical of soil. The objective of this study was to investigate the growth characteristics and inorganic nutrient contents of crops with application levels of phyllite.METHODS AND RESULTS: Both young radish and lettuce were selected as target crops for this study. The experiment was conducted in a wagner pot(1/5000a) in glass house at Sunchon National University. Treatment conditions were divided P0NPK(No phyllite + NPK), P5NPK(phyllite 5 Mg/ha + NPK), P10NPK(phyllite 10 Mg/ha + NPK) and P15NPK(phyllite 15 Mg/ha + NPK) by crops, respectively. Bulk density and porosity of soil in control without treatment conditions were ranged from 1.02 ∼1.04 g/cm³ and 56.5∼57.0%, respectively, and those for treatments with phyllite were in the ranged from 0.94∼1.00 g/cm³ and 58.4∼63.5%, respectively. Dry weights of young radish and lettuce were higher in P15NPK treatment than those in other treatments. The amounts of T-N, T-P and K uptake in young radish with phyllite application treatments were increased 36∼115, 18∼67 and 20∼76% than without phyllite application treatment, respectively. In lettuce treatments, amounts of T-N, T-P and K uptake were intended to all tested treatments similar with result of young radish treatment.CONCLUSION: Therefore, these results confirm that phyllite application to the soil improves physico-chemical of soil in addition to improving growth of young radish and lettuce.

• 한국콘텐츠학회논문지
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• 제13권6호
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• pp.236-245
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• 2013

본 연구는 노인자살증가의 원인을 파악하기 위해 거시적인 측면의 관점에서 노인자살률과 사회 경제적요인의 관련성을 분석하였다. 노인자살의 이론적 배경은 사회학적 관점과 경제학적 관점에 기초하여 설명하였다. 노인자살률의 경제적 요인에는 경제성장률과 실업률 그리고 노인의 경제활동참가율과 상대적 빈곤율(소득불평등율)을 포함하며, 사회적 요인의 범주에 속하는 변수로는 사회복지지출비, 이혼율, 그리고 고령화비 증가율과 노인부양비를 포함한다. 주요 연구결과로는 첫째, 노인의 경제활동참가율이 낮을수록 노인자살률이 높아지는 경향이 있었다. 둘째, 사회복지지출비가 낮을수록 노인자살률이 증가하는 것으로 나타났다. 셋째, 상대적 빈곤율, 고령화비 증가율, 그리고 노인부양비는 공히 이들이 증가할수록 노인자살률도 동시에 증가하는 것으로 나타났다. 끝으로 한국노인자살률의 지속적 증가를 예방하기 위해서 거시적 차원의 사회 경제적 정책방안을 제언하였다.